Consulta: creatorFacets:"Rouault, Karen"
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Analysis of the first genome of a hyperthermophilic marine virus-like particle, PAV1, isolated from Pyrococcus abyssi
Provedor de dados: 5
Autores: Geslin, Claire; Gaillard, Melusine; Flament, Didier; Rouault, Karen; Le Romancer, Marc; Prieur, Daniel; Erauso, Gael.
Only one virus-like particle (VLP) has been reported from hyperthermophilic Euryarchaeotes. This VLP, named PAV1 is shaped like a lemon and was isolated from a strain of "Pyrococcus abyssi," a deep-sea isolate. Its genome consists of a double-stranded circular DNA of 18 kb which is also present at a high copy number (60 per chromosome) free within the host cytoplasm but is not integrated into the host chromosome. Here, we report the results of complete analysis of the PAV1 genome. All the 25 predicted genes, except 3, are located on one DNA strand. A transcription map has been made by using a reverse transcription-PCR assay. All the identified open reading frames (ORFs) are transcribed. The most significant similarities relate to four ORFs. ORF 180a shows...
Tipo: Text
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3536.pdf