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Characterization of biotechnologically relevant extracellular lipase produced by Aspergillus terreus NCFT 4269.10
Provedor de dados: 58
Autores: Sethi,Bijay Kumar; Nanda,Prativa Kumari; Sahoo,Santilata.
Abstract Enzyme production by Aspergillus terreus NCFT 4269.10 was studied under liquid static surface and solid-state fermentation using mustard oil cake as a substrate. The maximum lipase biosynthesis was observed after incubation at 30 °C for 96 h. Among the domestic oils tested, the maximum lipase biosynthesis was achieved using palm oil. The crude lipase was purified 2.56-fold to electrophoretic homogeneity, with a yield of 8.44%, and the protein had a molecular weight of 46.3 kDa as determined by SDS-PAGE. Enzyme characterization confirmed that the purified lipase was most active at pH 6.0, temperature of 50 °C, and substrate concentration of 1.5%. The enzyme was thermostable at 60 °C for 1 h, and the optimum enzyme–substrate reaction time was 30...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus terreus; Lipase; Liquid static surface fermentation; Solid-state fermentation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100143