Consulta: creatorFacets:"Salles,L.P."
Registros recuperados: 2
Data/hora: 02/06/2024 03:55:49

Aspects of energetic substrate metabolism of in vitro and in vivo bovine embryos
Provedor de dados: 56
Autores: de Souza,D.K.; Salles,L.P.; Rosa e Silva,A.A.M.
Although the metabolism of early bovine embryos has not been fully elucidated, several publications have addressed this important issue to improve culture conditions for cattle reproductive biotechnologies, with the ultimate goal of producing in vitro embryos similar in quality to those developing in vivo. Here, we review general aspects of bovine embryo metabolism in vitro and in vivo, and discuss the use of metabolic analysis of embryos produced in vitro to assess viability and predict a viable pregnancy after transference to the female tract.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine pregnancy rates; Embryo metabolism; Metabolism profile; In vitro fertilization; Embryo culture conditions; In vivo embryo.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300191

Culture of bovine ovarian follicle wall sections maintained the highly estrogenic profile under basal and chemically defined conditions
Provedor de dados: 56
Autores: Vasconcelos,R.B.; Salles,L.P.; Oliveira e Silva,I.; Gulart,L.V.M.; Souza,D.K.; Torres,F.A.G.; Bocca,A.L.; Rosa e Silva,A.A.M.
Follicle cultures reproduce in vitro the functional features observed in vivo. In a search for an ideal model, we cultured bovine antral follicle wall sections (FWS) in a serum-free defined medium (DM) known to induce 17β-estradiol (E2) production, and in a nondefined medium (NDM) containing serum. Follicles were sectioned and cultured in NDM or DM for 24 or 48 h. Morphological features were determined by light microscopy. Gene expression of steroidogenic enzymes and follicle-stimulating hormone (FSH) receptor were determined by RT-PCR; progesterone (P4) and E2 concentrations in the media were measured by radioimmunoassay. DM, but not NDM, maintained an FWS morphology in vitro that was similar to fresh tissue. DM also induced an increase in the expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovary follicle wall section; FSH receptor; Gene expression; Steroidogenic enzymes; Hormone steroids; Morphological aspect.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800700