Consulta: creatorFacets:"Santiago-Vázquez,Lory Z"
Registros recuperados: 2
Data/hora: 09/06/2024 02:58:01

Suppression subtractive hybridization PCR isolation of cDNAs from a Caribbean soft coral
Provedor de dados: 69
Autores: Lopez,Jose V; Ledger,Angela; Santiago-Vázquez,Lory Z; Pop,Mihai; Sommer,Dan D; Ranzer,Llanie K; Feldman,Robert A; Russell,G. Kerr.
Transcriptomic studies of marine organisms are still in their infancy. A partial, subtracted expressed sequence tag (EST) library of the Caribbean octocoral Erythropodium caribaeorum and the sea fan Gorgonia ventalina has been analyzed in order to find novel genes or differences in gene expression related to potential secondary metabolite production or symbioses. This approach entails enrichment for potential non-&amp;#8220;housekeeping&amp;#8221; genes using the suppression subtractive hybridization (SSH) polymerase chain reaction (PCR) method. More than 500 expressed sequence tags (ESTs) were generated after cloning SSH products, which yielded at least 53 orthologous groups of proteins (COGs) and Pfam clusters, including transcription factors (Drosophila...
Tipo: Journal article
Palavras-chave: CDNA; Erythropodium caribaeorum; EST; Gorgonia ventalina; Gorgonian; Sea fan.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100008

A microbial community analysis of the octocoral Eunicea fusca
Provedor de dados: 69
Autores: Duque-Alarcón,Angela; Santiago-Vázquez,Lory Z; Kerr,Russell G.
While there is a significant and growing body of knowledge describing the microbial communities of marine invertebrates such as sponges, there are very few such studies focused on octocorals. The octocoral Eunicea fusca is common on reefs in various regions of the Caribbean and has been the subject of natural product investigations. As part of an effort to describe the microbial community associated with octocorals, a culture-independent analysis of the bacterial community of E. fusca was conducted. Specifically, a 16S rDNA clone library analysis was performed to provide baseline data. A total of 40 bacteria members from 11 groups were found. In general, Proteobacteria were the dominant group with a total of 24 species and &amp;#945;-Proteobacteria...
Tipo: Journal article
Palavras-chave: 16S rDNA; Bacteria; Culture-independent analysis; Eunicea fusca; Microbial community; Octocoral.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500015