Consulta: creatorFacets:"Scagliusi,Sandra Maria Mansur"
Registros recuperados: 3
Data/hora: 11/06/2024 11:25:36

Glutenin analysis for the safe screening of self-pollinated wheat seeds when developing doubled-haploid populations
Provedor de dados: 86
Autores: Torres,Gisele Abigail Montan; Scagliusi,Sandra Maria Mansur; Chaves,Márcia Soares; Consoli,Luciano.
Abstract: The objective of this work was to apply glutenin analysis, as a simple and reliable method, to identify self-pollinated wheat (Triticum aestivum) seeds in F1 crosses, when developing mapping populations using doubled-haploid (DH) technology. F1 seeds were subjected to glutenin analyses using gel electrophoresis, to verify and confirm their heterozygotic profiles. Glutenin analyses were carried out using one half of the endosperm of each of the 87 F1 seeds. The other half of the endosperm was used to develop the mapping population through DHs. Plants with band patterns different from the expected ones were discarded. Although the incidence of self-pollinated seeds was relatively low (only 8%), the screening of F1 heterozygotic profiles is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Puccinia recondita f. sp. tritici; Triticum aestivum; Adult plant resistance; Leaf rust; Microspore culture; Toropi wheat cultivar.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500646

Androgenic response of wheat genotypes resistant to fusariosis
Provedor de dados: 86
Autores: Coelho,Mônica Bossardi; Scagliusi,Sandra Maria Mansur; Lima,Maria Imaculada Pontes Moreira; Consoli,Luciano; Grando,Magali Ferrari.
Abstract: The objective of this work was to assess the androgenic response, via microspore culture, of wheat genotypes with different levels of resistance to Gibberella zeae. The number of androgenic embryos per spike, and of green and albino plants was counted for the BRS 179 (moderately resistant), Frontana and Sumai 3 (resistant), and BRS 194, Embrapa 27, and Fielder (susceptible) genotypes. The degree of interference by the Fielder, Pavon 76, and Sumai 3 ovary-donor genotypes, used for co-culture with the microspore cells, was also assessed regarding androgenic response. Induction efficiency ranged from 0.33 embryo per spike for Embrapa 27 to 109.8 embryos for Frontana. Sumai 3 presented the second best response, and Embrapa 27 behaved as highly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gibberella zeae; Triticum aestivum; Doubled haploids; Microspore.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500575

Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent
Provedor de dados: 86
Autores: Poersch-Bortolon,Liane Balvedi; Scagliusi,Sandra Maria Mansur; Yamazaki-Lau,Elene; Bodanese-Zanettini,Maria Helena.
Abstract The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plant regeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments: cold, at 4ºC for 21 days; and 2-hydroxynicotinic acid, at 32ºC for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Albinism; Androgenesis; Doubled haploid; Isolated microspore culture; Recalcitrance.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016001101839