Consulta: creatorFacets:"Schmitt, Paulina"
Registros recuperados: 13
Data/hora: 01/06/2024 03:33:24

Expression, tissue localization and synergy of antimicrobial peptides and proteins in the immune response of the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Schmitt, Paulina; De Lorgeril, Julien; Gueguen, Yannick; Destoumieux-garzon, Delphine; Bachere, Evelyne.
Diverse families of antimicrobial peptides and proteins have been described in oysters. We investigated here how antimicrobials are involved in the immune response against a pathogenic strain of Vibrio splendidus. Oyster antimicrobials were shown to display a wide variety of expression profiles in hemocyte populations and tissues. Oyster defensins are constitutively expressed in specific tissues such as mantle (Cg-Defm) or hemocytes (Cg-Defhs), while Cg-BPI is inducible and Cg-Prp appears down-regulated in hemocytes upon infection. The migratory behavior of hemocytes that express the different antimicrobials was found to be involved in the oyster response to a pathogenic Vibrio infection. Indeed, it contributes to colocalize several antimicrobials that...
Tipo: Text
Palavras-chave: Pacific oyster; Bivalve mollusk; Innate immunity; Bactericidal/permeability-increasing protein; Proline-rich peptide; Defensin; Vibrio.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00093/20382/18267.pdf

The new insights into the oyster antimicrobial defense: Cellular, molecular and genetic view
Provedor de dados: 5
Autores: Bachere, Evelyne; Rosa, Rafael Diego; Schmitt, Paulina; Poirier, Aurore; Merou, Nicolas; Charriere, Guillaume; Destoumieux-garzon, Delphine.
Oysters are sessile filter feeders that live in close association with abundant and diverse communities of microorganisms that form the oyster microbiota. In such an association, cellular and molecular mechanisms have evolved to maintain oyster homeostasis upon stressful conditions including infection and changing environments. We give here cellular and molecular insights into the Crassostrea gigas antimicrobial defense system with focus on antimicrobial peptides and proteins (AMPs). This review highlights the central role of the hemocytes in the modulation and control of oyster antimicrobial response. As vehicles for AMPs and other antimicrobial effectors, including reactive oxygen species (ROS), and together with epithelia, hemocytes provide the oyster...
Tipo: Text
Palavras-chave: Mollusk; Immunity; Antimicrobial peptide; Hemocyte; Defensin.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00254/36552/35097.pdf

Insight into Invertebrate Defensin Mechanism of Action OYSTER DEFENSINS INHIBIT PEPTIDOGLYCAN BIOSYNTHESIS BY BINDING TO LIPID II
Provedor de dados: 5
Autores: Schmitt, Paulina; Wilmes, Miriam; Pugniere, Martine; Aumelas, Andre; Bachere, Evelyne; Sahl, Hans-georg; Schneider, Tanja; Destoumieux-garzon, Delphine.
Three oyster defensin variants (Cg-Defh1, Cg-Defh2, and Cg-Defm) were produced as recombinant peptides and characterized in terms of activities and mechanism of action. In agreement with their spectrum of activity almost specifically directed against Gram-positive bacteria, oyster defensins were shown here to be specific inhibitors of a bacterial biosynthesis pathway rather than mere membrane-active agents. Indeed, at lethal concentrations, the three defensins did not compromise Staphylococcus aureus membrane integrity but inhibited the cell wall biosynthesis as indicated by the accumulation of the UDP-N-acetylmuramyl-pentapeptide cell wall precursor. In addition, a combination of antagonization assays, thin layer chromatography, and surface plasmon...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12503/9369.pdf

Sequence polymorphism and expression variability of Crassostrea gigas immune related genes discriminate two oyster lines contrasted in term of resistance to summer mortalities
Provedor de dados: 5
Autores: Schmitt, Paulina; Santini, Adrien; Vergnes, Agnes; Degremont, Lionel; De Lorgeril, Julien.
Summer mortalities of Crassostrea gigas are a major concern in oyster aquaculture. They are the result of a complex interaction between the host, pathogens and environmental factors. Oyster genetics have been identified as an essential determinant of oyster susceptibility to summer mortalities. As the capability of oysters to circumvent diseases depends in part on their immune defenses, we aimed to analyze the gene expression and sequence polymorphism of 42 immune related genes in two oyster lines selected for their “High” (H) and “Low” (L) survival to summer mortalities. Results showed that the variability of gene expression and the sequence polymorphism acting on particular genes could enable the discrimination between H and L oyster lines. Besides, a...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26861/24979.pdf

Diversité moléculaire des effecteurs antimicrobiens chez l'huître creuse Crassostrea gigas : mise en évidence et rôle dans la réponse antimicrobienne
Provedor de dados: 5
Autores: Schmitt, Paulina.
This work contributed to the knowledge of the molecular bases of oyster immunity by the characterization of the diversity of three antimicrobials of C. gigas and the understanding of the role played by their diversity in the oyster antimicrobial response. Phylogenetic analyses of two antimicrobial peptides (AMPs), Cg-Defensins (Cg-Defs) and Cg-Proline rich peptide (Cg-Prp), and one Bactericidal Permeability Increasing protein, Cg-BPI, led us to the identification of a high diversity for both AMPs. Further analyses showed that this diversity is generated by gene duplication, allelic recombination and directional selection pressures, suggesting their functional diversification. The biological meaning of AMP diversity was investigated for the three major...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00028/13906/11072.pdf

Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin
Provedor de dados: 5
Autores: Gueguen, Yannick; Romestand, Bernard; Fievet, Julie; Schmitt, Paulina; Destoumieux Garzon, Delphine; Franck, Vandenbulcke; Philippe, Bulet; Bachere, Evelyne.
A cDNA sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (AMPs) was identified in the oyster Crassostrea gigas. After release of a hydrophobic signal peptide. the resulting 37-amino acid peptide, Cg-Prp, is composed of an acidic region and a cationic proline-rich region. To evaluate the biological properties of Cg-Prp, multiple proline-rich peptides corresponding to putative processing of the full-length Cg-Prp were synthesized. A limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with Cg-Def, a defensin from C gigas. To our knowledge, this is the first evidence of synergy between a defensin and another AMP in an...
Tipo: Text
Palavras-chave: Antimicrobial peptide; Mollusk; Synergy; Invertebrate; Innate immunity; Pacific oyster.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6232.pdf

Resistance to Antimicrobial Peptides in Vibrios
Provedor de dados: 5
Autores: Destoumieux-garzón, Delphine; Duperthuy, Marylise; Vanhove, Audrey; Schmitt, Paulina; Wai, Sun.
Vibrios are associated with a broad diversity of hosts that produce antimicrobial peptides (AMPs) as part of their defense against microbial infections. In particular, vibrios colonize epithelia, which function as protective barriers and express AMPs as a first line of chemical defense against pathogens. Recent studies have shown they can also colonize phagocytes, key components of the animal immune system. Phagocytes infiltrate infected tissues and use AMPs to kill the phagocytosed microorganisms intracellularly, or deliver their antimicrobial content extracellularly to circumvent tissue infection. We review here the mechanisms by which vibrios have evolved the capacity to evade or resist the potent antimicrobial defenses of the immune cells or tissues...
Tipo: Text
Palavras-chave: Vibrio; Lipopolysaccharide; Outer membrane vesicle; Membrane transporter; Innate immunity; Defensin; Cathelicidin; Bactericidal/permeability-increasing protein.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00276/38767/37308.pdf

An intimate link between antimicrobial peptide sequence diversity and binding to essential components of bacterial membranes
Provedor de dados: 5
Autores: Schmitt, Paulina; Rosa, Rafael D.; Destoumieux-garzon, Delphine.
Antimicrobial peptides and proteins (AMPs) are widespread in the living kingdom. They are key effectors of defense reactions and mediators of competitions between organisms. They are often cationic and amphiphilic, which favors their interactions with the anionic membranes of microorganisms. Several AMP families do not directly alter membrane integrity but rather target conserved components of the bacterial membranes in a process that provides them with potent and specific antimicrobial activities. Thus, lipopolysaccharides (LPS), lipoteichoic acids (LTA) or the peptidoglycan precursor Lipid II are targeted by a broad series of AMPs. Studying the functional diversity of immune effectors tells us about the essential residues involved in AMP mechanism of...
Tipo: Text
Palavras-chave: Functional diversity; Defensin; Anti-lipopolysaccharide factor; Mechanism of action; Resistance.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00286/39710/38165.pdf

Massive Gene Expansion and Sequence Diversification Is Associated with Diverse Tissue Distribution, Regulation and Antimicrobial Properties of Anti-Lipopolysaccharide Factors in Shrimp
Provedor de dados: 5
Autores: Matos, Gabriel Machado; Schmitt, Paulina; Barreto, Caire; Farias, Natanael Dantas; Toledo-silva, Guilherme; Guzman, Fanny; Destoumieux Garzon, Delphine; Perazzolo, Luciane Maria; Rosa, Rafael Diego.
Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides with a central β-hairpin structure able to bind to microbial components. Mining sequence databases for ALFs allowed us to show the remarkable diversity of ALF sequences in shrimp. We found at least seven members of the ALF family (Groups A to G), including two novel Groups (F and G), all of which are encoded by different loci with conserved gene organization. Phylogenetic analyses revealed that gene expansion and subsequent diversification of the ALF family occurred in crustaceans before shrimp speciation occurred. The transcriptional profile of ALFs was compared in terms of tissue distribution, response to two pathogens and during shrimp development in Litopenaeus vannamei, the most...
Tipo: Text
Palavras-chave: Host defense peptide; Antimicrobial peptide; Anti-LPS factor; Hostmicrobe relationship; Functional diversity; Invertebrate immunity; Crustacean; Antimicrobial activity.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00464/57523/59711.pdf

Use of OmpU porins for attachment and invasion of Crassostrea gigas immune cells by the oyster pathogen Vibrio splendidus
Provedor de dados: 5
Autores: Duperthuy, Marylise; Schmitt, Paulina; Garzon, Edwin; Caro, Audrey; Rosa, Rafael D.; Le Roux, Frederique; Lautredou-audouy, Nicole; Got, Patrice; Romestand, Bernard; De Lorgeril, Julien; Kieffer-jaquinod, Sylvie; Bachere, Evelyne; Destoumieux-garzon, Delphine.
OmpU porins are increasingly recognized as key determinants of pathogenic host Vibrio interactions. Although mechanisms remain incompletely understood, various species, including the human pathogen Vibrio cholera, require OmpU for host colonization and virulence. We have shown previously that OmpU is essential for virulence in the oyster pathogen Vibrio splendidus LGP32. Here, we showed that V. splendidus LGP32 invades the oyster immune cells, the hemocytes, through subversion of host-cell actin cytoskeleton. In this process, OmpU serves as an adhesin/invasin required for beta-integrin recognition and host cell invasion. Furthermore, the major protein of oyster plasma, the extracellular superoxide dismutase Cg-EcSOD, is used as an opsonin mediating the...
Tipo: Text
Palavras-chave: Host-pathogen interaction; Innate immunity; Invertebrate; Mollusk; Oxidative burst.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00031/14177/12166.pdf

Litopenaeus vannamei stylicins are constitutively produced by hemocytes and intestinal cells and are differentially modulated upon infections
Provedor de dados: 5
Autores: Farias, Natanael Dantas; Falchetti, Marcelo; Matos, Gabriel Machado; Schmitt, Paulina; Barreto, Cairé; Argenta, Nicolas; Rolland, Jean-luc; Bachère, Evelyne; Perazzolo, Luciane Maria; Rosa, Rafael Diego.
Stylicins are anionic antimicrobial host defense peptides (AAMPs) composed of a proline-rich N-terminal region and a C-terminal portion containing 13 conserved cysteine residues. Here, we have increased our knowledge about these unexplored crustacean AAMPs by the characterization of novel stylicin members in the most cultivated penaeid shrimp, Litopenaeus vannamei. We showed that the L. vannamei stylicin family is composed of two members (Lvan-Stylicin1 and Lvan-Stylicin2) encoded by different loci which vary in gene copy number. Unlike the other three gene-encoded antimicrobial peptide families from penaeid shrimp, the expression of Lvan-Stylicins is not restricted to hemocytes. Indeed, they are also produced by the columnar epithelial cells lining the...
Tipo: Text
Palavras-chave: Crustacean; Penaeid shrimp; Invertebrate immunity; Antimicrobial peptide; Host defense peptide; Molecular diversity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00466/57816/60102.pdf

Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas.
Provedor de dados: 5
Autores: Poirier, Aurore C.; Schmitt, Paulina; Rosa, Rafael D.; Vanhove, Audrey S.; Kieffer-jaquinod, Sylvie; Rubio, Tristan P.; Charriere, Guillaume M.; Destoumieux-garzon, Delphine.
Although antimicrobial histones have been isolated from multiple metazoan species, their role in host defense has long remained unanswered. We found here that the hemocytes of the oyster Crassostrea gigas release antimicrobial H1-like and H5-like histones in response to tissue damage and infection. These antimicrobial histones were shown to be associated with extracellular DNA networks released by hemocytes, the circulating immune cells of invertebrates, in response to immune challenge. The hemocyte-released DNA was found to surround and entangle vibrios. This defense mechanism is reminiscent of the neutrophil extracellular traps (ETs) recently described in vertebrates. Importantly, oyster ETs were evidenced in vivo in hemocyte-infiltrated interstitial...
Tipo: Text
Palavras-chave: Antimicrobial Peptide (AMP); DNA; Innate Immunity; Invertebrate; Reactive Oxygen Species (ROS); NET; Mollusk.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00217/32866/31784.pdf

Molecular diversity of antimicrobial effectors in the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Schmitt, Paulina; Gueguen, Yannick; Desmarais, Erick; Bachere, Evelyne; De Lorgeril, Julien.
Background: To gain insight into the molecular diversity of antimicrobial peptides and proteins in the oyster Crassostrea gigas, we characterized and compared the sequence polymorphism of the antimicrobial peptides (AMPs), Cg-Defensins (Cg-Defs) and Cg-Proline Rich peptide (Cg-Prp), and of the bactericidal permeability increasing protein, Cg-BPI. For that, we analyzed genomic and transcript sequences obtained by specific PCR amplification and in silico searches. Results: High diversification among the three antimicrobial effectors was evidenced by this polymorphism survey. On the basis of sequence phylogenies, each AMP aggregates into clearly defined groups of variants and is the product of a multigenic family displaying a variety of gene structures. In...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00001/11182/6691.pdf