Consulta: creatorFacets:"Shirafuji, Hiroaki"
Registros recuperados: 2
Data/hora: 11/06/2024 13:58:34

DNA vaccine coding a p23 protein of Cryptosporidium parvum fused with Fc portion of immunogloblin G induces higher level of interferon-γ expression in mice
Provedor de dados: 19
Autores: Takashima, Yasuhiro; Xuan, Xuenan; Shirafuji, Hiroaki; Zhang, Guohong; Otsuka, Haruki.
To develop DNA vaccines against Cryptosporidiosis, a plasmid coding an immunodominant protein of Cryptosporidium parvum sporozoite, p23 (pCX-p23) and another plasmid coding a fusion protein containing whole the p23 and the Fc portion of mouse immunogloblin G1 (pCX-p23Fc). Vaccination of BALB/c mice with the plasmid, pCX-p23 and pCX-p23Fc induced the production of antibodies against p23. Although both of splenocytes of mice immunized with the plasmids pCX-p23 and pCX-p23Fc expressed interleukin-4 and interferon-8, after the in vitro stimulation by p23 antigen, the interferon-8 expression level of pCX-p23Fc immunized mice was much higher than that of pCX-p23 immunized mice. These results suggest a possibility  of the plasmid pCX-p23Fc as a DNA vaccine...
Palavras-chave: C. parvum; DNA vaccine; Fc; Interferon-8.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/147

Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii
Provedor de dados: 19
Autores: Liao, Min; Xuan, Xuenan; Huang, Xiaohong; Shirafuji, Hiroaki; Fukumoto, Shinya; Hirata, Haruyuki; Suzuki, Hiroshi; Fujisaki, Kozo.
Murine monoclonal antibodies (mAbs) against Neospora caninum tachyzoites were produced to identify the cross-reactive antigens between N. caninum and Toxoplasma gondii. Ten mAbs recognizing cross-reactive antigens of both parasites were obtained and tentatively classified into 6 different groups based on their reactivity patterns in an indirect fluorescent antibody test and Western blot analysis. Three mAbs in group I recognized antigens located on the surface of parasites with molecular masses ranging from 28 to 76 kDa; one mAb in group 2 recognized antigens located on interior organelles of parasites with a molecular mass of 50 kDa; one mAb in group 3 recognized antigens located on interior organelles of parasites with molecular masses of 35 kDa and 14...
Palavras-chave: Neospora caninum; Toxoplasma gondii; Cross-reactive antigen; Protein disulfide isomerase; Heat-shock protein; Ribosomal protein 1.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/816