Consulta: creatorFacets:"Silva,A.P.C."
Registros recuperados: 3
Data/hora: 14/06/2024 08:22:49

Indirect ELISA for diagnosis of Brucella ovis infection in rams
Provedor de dados: 45
Autores: França,S.A.; Mol,J.P.S.; Costa,E.A.; Silva,A.P.C.; Xavier,M.N.; Tsolis,R.M.; Reis,J.K.P.; Paixão,T.A.; Santos,R.L.
Brucella ovis is a major cause of epididymitis in sexually mature rams, resulting in subfertility, infertility, and economic losses for the sheep industry worldwide. The aim of this study was to develop an indirect ELISA (iELISA) using recombinant proteins, namely rBoP59 and rBP26, as antigens for serological diagnosis of B. ovisinfection. The BoP59 and BP26 recombinant proteins were expressed in E. coli and purified by affinity chromatography. Antigenicity was tested by Western blot and iELISA. Standardization of iELISA was performed with 500ng and 1µg BoP59 and rBP26 per well, testing serum from uninfected and experimentally infected rams. rBP26 was effective in distinguishing positive from negative rams. The rBP26 iELISA developed in this study is the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucella ovis; Brucellosis; ELISAi; BP26; Western blot.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601695

Dermatosparaxis in two White Doper lambs in Brazil: case report
Provedor de dados: 45
Autores: Silva,A.P.C.; Mol,J.P.S.; Carvalho Junior,C.A.; Paixão,T.A.; Santos,R.L.
Dermatosparaxis is a genetic disease that affects collagen maturation. This disease is characterized by marked impairment of the resistance of collagen fibers that leads to skin fragility, and it may affect several species. This is the first report of dermatosparaxis in sheep in Brazil. Clinically, two White Dorper lambs had multiple skin lacerations in the neck, dorsum and limbs. Transmission microscopy demonstrated irregular collagen fibers arranged in hieroglyphic shape and scanning electron microscopy demonstrated thin collagen fibrils that were not arranged in bundles as observed in the normal dermis. These findings are consistent with the diagnosis of dermatosparaxis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sheep; Dermatosparaxis; Collagen; Skin.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300741

Chronic intestinal pseudo-obstruction in a dog: case report
Provedor de dados: 45
Autores: Bicalho,A.L.; Silva,A.P.C.; Paixão,T.A.; Cardoso Jr.,R.B.; Santos,R.L.
Intestinal pseudo-obstruction is a rare disorder that affects gastrointestinal propulsion. It may be secondary to several pathological conditions or it may develop without a known cause. A 1.2 year-old intact Pug bitch had a history of vomiting and constipation, which were followed by diarrhea and distended abdomen. Hypomotility and dilation of the small intestine, which was filled with gas, were observed during laparotomy. Histologically, full thickness biopsy specimens demonstrated a severe loss and degeneration of leiomyocytes in the inner and outer muscular layers of the intestinal wall, whereas there was a marked hypertrophy and hyperplasia of smooth muscle cells in the lamina propria, and extremely thickened muscularis mucosae arranged in bundles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Pseudo-obstruction; Intestine.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600007