Consulta: creatorFacets:"Silva,Francielle Gibson da"
Registros recuperados: 3
Data/hora: 01/06/2024 02:39:49

Leptospires detection in kidney, liver and uterus of cows slaughtered in Paraná State, Brazil
Provedor de dados: 58
Autores: Silva,Francielle Gibson da; Freitas,Julio Cesar de; Anzai,Eleine Kuroki; Hashimoto,Vanessa Yumi; Giraldi,Nilson; Delbem,Ádina Cléia B.; Bracarense,Ana Paula F.R.L.; Reis,Antônio Carlos F. dos; Vasconcellos,Silvio Arruda.
The objective of this trial was to detect leptospires in fragments of kidney, liver and uterus of 96 cows with unknown sanitary status, randomly chosen at slaughter in Paraná, Brazil. All 96 urine samples were submitted to direct examination using dark field microscopy. Positive samples in the direct examination and all kidney, liver and uterus fragments were cultured in EMJH modified medium and Tween 80/40/LH. Sections cut of kidney, liver and uterus were stained by Hematoxylin-Eosin (HE) and indirect immunoperoxidase with hyperimmune serum against serovar Hardjo (Hardjoprajitno) and strain Londrina 14 (LO14), from serovar Canicola, which was isolated in the northern region of Paraná state, Brazil, used as primary antibodies. Direct examination detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospire; Indirect immunoperoxidase; Immunohistochemistry; Cows; Culture.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100008

Isolation of Leptospira spp from dogs, bovine and swine naturally infected
Provedor de dados: 65
Autores: Freitas,Julio Cesar de; Silva,Francielle Gibson da; Oliveira,Rosângela Claret de; Delbem,Ádina Cléia Botazzo; Müller,Ernst Eckehardt; Alves,Lucimara Aparecida; Teles,Paulo Sergio.
Leptospira isolation allows definitive diagnosis of the infection. Contamination by microorganisms is one of the inconveniences of the culture. The objective of this study was to describe the isolation of Leptospira from dogs, bovine and swine naturally infected. Urine samples from 14 dogs and three bovines, and kidney, liver, ovary, and uterus body samples from 36 slaughtered sows with unknown health history, were used. The urine and organ samples were cultured in culture medium. Modified Ellinghausen-McCullough-Johnson-Harris medium (EMJH) culture medium was used with addition of 5-fluorouracil, chloramphenicol, vancomycin, nalidixic acid and neomycin. Incubation was performed at 28oC for 24 hours, followed by subculture in modified EMJH without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Diagnostic; Culture; Cattle; Dog; Swine.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300030

Chlamydophila abortus em animais de produção
Provedor de dados: 65
Autores: Silva,Francielle Gibson da; Freitas,Julio Cesar de; Müller,Ernst Eckehardt.
A Chlamydophila abortus (anteriormente classificada como Chlamydia psittaci sorotipo 1) tem sido descrita em muitos países, associada principalmente com distúrbios reprodutivos em ovinos, bovinos e caprinos. O aborto enzoótico dos ovinos e caprinos e o aborto epizoótico dos bovinos são as doenças mais importantes causadas por esta bactéria. No Brasil, as pesquisas com C. abortus são praticamente inexistentes. O objetivo desta revisão é apresentar informações sobre modificações taxonômicas, ciclo de vida, epidemiologia, patogenia, sinais clínicos e diagnóstico da infecção por C. abortus principalmente em ovinos, bovinos e caprinos.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlamydophila abortus; Ovino; Bovino; Caprino; Aborto; Problema reprodutivo.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100057