Consulta: creatorFacets:"Silva,Rodrigo Otávio Silveira"
Registros recuperados: 31
Data/hora: 09/06/2024 13:25:56

Antimicrobial susceptibility of Clostridium perfringens isolated from piglets with or without diarrhea in Brazil
Provedor de dados: 58
Autores: Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Pires,Prhiscylla Sadanã; Cruz Júnior,Eduardo Coulaud da Costa; Albefaro,Isabella Silva; Guedes,Roberto Maurício de Carvalho; Lobato,Francisco Carlos Faria.
The minimum inhibitory concentration (MIC) was determined for 13 antibiotics against Clostridium perfringens isolated from Brazilian piglets. The collection of isolates was performed in June to October 2010. All isolates were susceptible to amoxicillin and ceftiofur, whereas most were resistant to tetracycline and lincomycin. Avilamycin and narasin were more effective against isolates from non-diarrheic than from diarrheic piglets. The other antimicrobials were less active in need of high concentrations to inhibit the growth of the C. perfringens type A. These results suggest the need for further studies evaluating molecular factors related to the antimicrobial resistance of C. perfringens.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Piglet; Diarrhea; Antibiotic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300027

Detection of toxins A/B and isolation of Clostridium difficile and Clostridium perfringens from dogs in Minas Gerais, Brazil
Provedor de dados: 58
Autores: Silva,Rodrigo Otávio Silveira; Santos,Renata Lara Resende; Pires,Prhiscylla Sadanã; Pereira,Luiz Carlos; Pereira,Silvia Trindade; Duarte,Marina Carvalho; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria.
The objective of this study was to detect C. difficileA/B toxins and to isolate strains of C. perfringensand C. difficile from diarrheic and non-diarrheic dogs in Brazil. Stool samples were collected from 57 dogs, 35 of which were apparently healthy, and 22 of which were diarrheic. C. difficileA/B toxins were detected by ELISA, and C. perfringensand C. difficilewere identified by multiplex PCR. C. difficileA/B toxins were detected in 21 samples (36.8%). Of these, 16 (76.2%) were from diarrheic dogs, and five (23.8%) were from non-diarrheic dogs. Twelve C. difficile strains (21.1%) were isolated, of which ten were A+B+and two were A-B-. All non-toxigenic strains were isolated from non-diarrheic animals. The binary toxin gene cdtBwas found in one strain,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nosocomial diarrhea; Small animals; Colitis; Enteritis; Canine.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100019

Mapping of the continuous epitopes displayed on the Clostridium perfringens type D epsilon-toxin
Provedor de dados: 58
Autores: Alves,Guilherme Guerra; Machado-de-Ávila,Ricardo Andrez; Chávez-Olórtegui,Carlos Delfin; Silva,Rodrigo Otávio Silveira; Lobato,Francisco Carlos Faria.
Abstract The epsilon toxin, produced by Clostridium perfringens, is responsible for enterotoxemia in ruminants and is a potential bioterrorism agent. In the present study, 15 regions of the toxin were recognized by antibodies present in the serum, with different immunodominance scales, and may be antigen determinants that can be used to formulate subunit vaccines.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epsilon; Toxin; Enterotoxemia; Epitope; Mapping.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300570

Histopathological, immunohistochemical and biomolecular diagnosis of myocarditis due to Clostridium chauvoei in a bovine
Provedor de dados: 65
Autores: Casagrande,Renata Assis; Pires,Prhiscylla Sadanã; Silva,Rodrigo Otávio Silveira; Sonne,Luciana; Borges,João Batista Souza; Neves,Monique Silva; Rolim,Veronica Machado; Souza,Suyene Oltramari de; Driemeier,David; Lobato,Francisco Carlos Faria.
The aim of this study was to report a case of clostridial myocarditis in a bovine in Brazil with emphasis on the pathological findings, isolation and molecular identification associated with the in situ localization of C. chauvoei. The animal, a male Brangus bull with nine months of age, was found dead without prior clinical signs. Multifocal and coalescent areas of necrosis were observed in the myocardium. Rod cells in the cardiac muscle fibers were positive immunostaining for C. chauvoei, while this bacterium was also isolated and identified by polymerase chain reaction (PCR).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myonecrosis; Sudden death; Clostridium chauvoei; Myocarditis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801472

Jejunal hemorrhage syndrome in a Zebu cow in Brazil
Provedor de dados: 65
Autores: Pires,Prhiscylla Sadanã; Uribe,Jose Azael Zambrano; Carvalho,Antônio Último de; Silva,Rodrigo Otávio Silveira; Salvarani,Felipe Masiero; Meneses,Rodrigo Melo; Leite,Luiza Bossi; Alves,Guilherme Guerra; Gonçalves,Luciana Aramuni; Lobato,Francisco Carlos Faria; Facury Filho,Elias Jorge.
Clostridium perfringenstype A has been incriminated as the etiologic agent in jejunal hemorrhage syndrome (JHS), which is a disease that affects dairy cattle. Although this microorganism is considered an important enteropathogen the pathogenesis of JHS is still not clear, and there have been no reports of its occurrence in Brazil so far. The aim of this study was to describe the occurrence of JHS by infection with a C. perfringenstype A strain carrying the beta-2 toxin gene in a zebu cow in Brazil, for the first time.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Beta-2 toxin gene; Enteropathogen; Enteric blood clot.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801476

First confirmed case of Clostridium difficile-associated diarrhea in foals in Brazil
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Moreira,Felipe Moraghi; Rezende,Jackeline Viana; Pires,Prhiscylla Sadanã; Maranhão,Renata de Pino Albuquerque; Palhares,Maristela Silveira; Lobato,Francisco Carlos Faria.
Despite of the substantial role of Clostridium difficile in causing diarrhea and colitis in foals, there have been no confirmed diagnoses of disease caused by this bacteria in Brazil. In this paper, we describe confirmed cases of colitis caused by C. difficile in two foals in Brazil. Two five-month-old foals with a five-day history of diarrhea after antibiotic treatment for a respiratory disease were treated at the Veterinary Hospital of the Universidade Federal de Minas Gerais. C. difficile A/B toxins were detected, and toxigenic strains of C. difficile were isolated from the foals' feces. The treatment was based on fluid therapy and antibiotics (metronidazole and ceftiofur), and the animals experienced a gradual recovery. The association between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colitis; Equine; Nosocomial diarrhea.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000300018

Antimicrobial susceptibility of Clostridium difficile isolated from animals and humans in Brazil
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Diniz,Amanda Nádia; Alves,Guilherme Guerra; Guedes,Roberto Maurício Carvalho; Vilela,Eduardo Garcia; Lobato,Francisco Carlos Faria.
The objective of this study was to evaluate antimicrobial susceptibility in Clostridium difficile strains isolated from animals and humans in Brazil. The 54 C. difficile strains used were isolated from stool samples from piglets (n=16), dogs (n=13), humans (n=13), foals (n=8) calves (n=2), an ocelot (n=1) and a maned wolf (n=1). Antimicrobial susceptibility was determined using the serial plate agar dilution method for penicillin, florfenicol, oxytetracycline, erythromycin, vancomycin, metronidazole and tylosin. The C. difficile strains assessed were susceptible to metronidazole and vancomycin. Florfenicol resistance was rarely observed; 52 (96.4%) strains were sensitive to this antimicrobial. Five (9.3%), five (9.3%), 14 (25.9%) and 20 (37.0%) strains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nosocomial diarrhea; Pseudomembranous colitis; Resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000500013

Outbreak of multidrug resistant Salmonella Typhimurium in calves at a veterinary hospital in Brazil
Provedor de dados: 65
Autores: Ramos,Carolina Pantuzza; Vespasiano,Letícia Camêlo; Melo,Isabela Oliveira; Xavier,Rafael Gariglio Clark; Leal,Carlos Augusto Gomes; Facury Filho,Elias Jorge; Carvalho,Antônio Ultimo de; Lobato,Francisco Carlos Faria; Silva,Rodrigo Otávio Silveira.
ABSTRACT: The present study aimed to describe and characterize a nosocomial outbreak caused by a multidrug resistant Salmonella Typhimurium in hospitalized calves at a veterinary medical teaching hospital from Brazil. Sixty-three (96.9%) calves showed lethargy, hyperthermia and profuse diarrhea and despite treatment, 26 (41.2%) animals died. Five animals were necropsied and stool samples of six calves were collected. The isolated strains were subjected to antimicrobial susceptibility test by disc-difusion method and were fingerprinted by ERIC-PCR. Macroscopic lesions suggestive of salmonellosis, such as fibrinonecrotic enteritis and hepatosplenomegaly were observed. Salmonellosis was confirmed by isolation of S. Typhimurium from stool samples and organs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonellosis; Bovine; Nosocomial; Zoonosis; Multiresistant.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200452

Detection of enterotoxin A and cytotoxin B, and isolation of Clostridium difficile in piglets in Minas Gerais, Brazil
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Salvarani,Felipe Masiero; Cruz Júnior,Eduardo Coulaud da Costa; Pires,Prhiscylla Sadanã; Santos,Renata Lara Resende; Assis,Ronnie Antunes de; Guedes,Roberto Maurício de Carvalho; Lobato,Francisco Carlos Faria.
Clostridium difficile has emerged as a major cause of neonatal colitis in piglets, displacing classic bacterial pathogens. However, there is no information regarding the distribution of this microorganism in pig farms in Brazil. In the present study, the presence of toxins A/B and of C. difficile strains in stool samples from 60 diarrheic or non-diarrheic newborn piglets (one to seven days old), from 15 different farms, was studied. The presence of toxins A/B was detected by ELISA and PCR was used to identify toxin A, toxin B and binary toxin gene in each isolated strain. C. difficile A/B toxins were detected in ten samples (16.7%). Of these, seven were from diarrheic and three were from non-diarrheic piglets. C. difficile was recovered from 12 out of 60...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonatal diarrhea; Clostridium difficile; Enteritis; Colitis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800021

Botulism in ruminants in Brazil
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Gonçalves,Luciana Aramuni; Lobato,Francisco Carlos Faria.
ABSTRACT: Botulism is an intoxication caused by the ingestion of neurotoxins secreted by  Clostridium botulinum. It affects all warm-blooded animals and some fishes and is characterized by progressive flaccid symmetrical paralysis. Despite the known importance of this disease in ruminants, the Brazilian literature only includes case studies and outbreak descriptions. In addition, the epidemiology of botulism in cattle has markedly changed over the past two decades. The aim of this study is to review these characteristics and to provide recent data on the occurrence of botulism in ruminants in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flaccid paralysis; Clostridium botulinum; Cattle; Buffaloes; Goats; Sheep.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801411

Botulismo tipo C em perus em Minas Gerais, Brasil
Provedor de dados: 65
Autores: Lobato,Francisco Carlos Faria; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Assis,Ronnie Antunes de; Lago,Luis Alberto do; Carvalho Filho,Maurício Baltazar de; Martins,Nelson Rodrigo da Silva.
O botulismo é uma intoxicação causada pela ingestão das toxinas produzidas pelo Clostridium botulinum, que acomete mamíferos e aves e é caracterizado por um quadro de paralisia flácida. Neste trabalho, é descrito um surto de botulismo em perus, ocorrido no município de Santa Luzia, região metropolitana de Belo Horizonte, Minas Gerais. Os animais apresentavam incoordenação motora, paralisia flácida das patas, asas e pescoço. Em um intervalo de 24 horas, todos os 29 animais do plantel vieram a óbito. Na necropsia, observou-se a presença de larvas de mosca no inglúvio. Nos soros coletados, foi identificada a toxina botulínica tipo C pelo teste de soroneutralização em camundongos.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxina botulínica tipo C; Clostridium botulinum; Perus; Aves.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100047

Detection of Lawsonia intracellularis fecal shedding in dogs in Minas Gerais, Brazil
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Gabardo,Michelle de Paula; Oliveira,Juliana Saes Vilaça de; Lobato,Francisco Carlos Faria; Guedes,Roberto Maurício Carvalho.
Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lawsonia intracellularis; Canine; Proliferative enteropathy; Diarrhea.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000901619

A retrospective study on the diagnosis of clostridial myonecrosis in ruminants in Brazil
Provedor de dados: 65
Autores: Pires,Prhiscylla Sadanã; Ecco,Roselene; Silva,Rodrigo Otávio Silveira; Araújo,Marina Rios de; Salvarani,Felipe Masiero; Heneine,Luíz Guilherme Dias; Oliveira Júnior,Carlos Augusto de; Lobato,Francisco Carlos Faria.
ABSTRACT: A standardized immunochemistry method for the diagnosis of clostridial myonecrosis was applied to 38 formalized tissue samples from ruminants with clinical and post mortem history suggestive of blackleg or gas gangrene. The diagnosis of clostridial myonecrosis was confirmed in 37 out of 38 (97.4%) samples tested. Clostridium chauvoei and Clostridium perfringens type A were the most common agents found alone, being detected in ten (26.3%) and six (15.8%) samples, respectively. The other cases showed an association of two or three clostridia, with C. perfringens type A detected in 11 (29%) cases. Based on the findings of the present study, polyvalent vaccines against clostridial infections of animals incorporating C. perfringens would be more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gas gangrene; Blackleg; Malignant edema; Cattle; Sheep; Immunohistochemistry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100452

Produção e caracterização de anticorpos monoclonais contra toxina épsilon de Clostridium perfringens Tipo D
Provedor de dados: 65
Autores: Freitas,Theonys Diógenes; Heneine,Luis Guilherme Dias; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Lima,Catarina Guimarães Rocha Dourado; Pires,Prhiscylla Sadanã; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria.
Clostridium perfringens tipo D é o agente etiológico da enterotoxemia em ruminantes, causada pela toxina épsilon e caracterizada por edema cardíaco, pulmonar, renal e cerebral. Anticorpos monoclonais contra toxina épsilon de C. perfringens tipo D foram produzidos a partir da fusão da linhagen de mieloma P3-X63-Ag8 653 com células do baço de camundongos Balb/c imunizados com o toxóide épsilon. Seis linhagens de híbridos secretores de anticorpos monoclonais das classes e IgM e IgG foram estabelecidas.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Toxina épsilon; Anticorpos monoclonais.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100046

Microbiological quality of honey from stingless bee, jandaíra (Melipona subnitida), from the semiarid region of Brazil
Provedor de dados: 65
Autores: Pinheiro,Carolina de Gouveia Mendes da Escóssia; Abrantes,Maria Rociene; Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Lobato,Francisco Carlos Faria; Silva,Jean Berg Alves da.
ABSTRACT:  The aim of this study was to evaluate the presence of microorganisms in honey produced by the stingless bee (SB) jandaíra (Melipona subnitida) from the semiarid region of Brazil. Thirty-five samples were analyzed and all of them were positive for mesophilic bacteria, coliforms at 45°C, fungi, and yeast. Staphylococcus spp. were identified in 85.7%, while Bacillus spp. were detected in 34.3% of honey samples. DNAs of Clostridium perfringens and C. botulinum were detected in 40% and 2.8% of the samples, respectively. Salmonella spp. and C. difficile were not detected. The present research revealed a great diversity of microorganisms in honey produced by jandaíra.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium botulinum; Clostridium perfringens; Stingless bee.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900450

Antimicrobial susceptibility and molecular characterization of Salmonella serovar Ndolo isolated from outbreaks in cattle and horses
Provedor de dados: 65
Autores: Ramos,Carolina Pantuzza; Xavier,Rafael Gariglio Clark; Leal,Carlos Augusto Gomes; Facury Filho,Elias Jorge; Carvalho,Antonio Ultimo de; Viegas,Flávia Mello; Pires,Isadora Honorato; Lopes,Emily Oliveira; Lobato,Francisco Carlos Faria; Silva,Rodrigo Otávio Silveira.
ABSTRACT:  The present study aimed to describe and characterize, for the first time, two outbreaks of salmonellosis caused by Salmonella Ndolo in foals and calves in Brazil and compare the isolated strains with S. Ndolo previously identified in asymptomatic reptiles. The affected calves and foals presented fever, lethargy, and profuse diarrhea. Isolated strains were subjected to antimicrobial susceptibility testing, characterized according to virulence genes, and fingerprinted by ERIC-PCR. Salmonella Ndolo was identified in fecal samples from two foals and four calves. One isolate from a calf was resistant to amoxicillin/clavulanic acid, trimethoprim/sulfamethoxazole, and florfenicol. Strains from two other calves were resistant to oxytetracycline. All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonellosis; Zoonoses; Antimicrobial resistance; ERIC-PCR; Salmonella Ndolo.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200452

Botulism in non-ruminants in Brazil
Provedor de dados: 65
Autores: Oliveira Júnior,Carlos Augusto de; Silva,Rodrigo Otávio Silveira; Olinda,Roberio Gomes; Lobato,Francisco Carlos Faria.
ABSTRACT: Botulism is an intoxication caused by the ingestion of neurotoxins secreted by Clostridium botulinum and characterized by progressive flaccid symmetrical paralysis. Among non-ruminant animals, avian species and dogs are the most commonly affected by botulism, while horses and pigs are less-commonly diagnosed with the disease. Despite the importance of this disease in animals, the Brazilian literature only includes case studies and outbreak descriptions. The aim of this study was to review the main features and to provide recent data on the occurrence of botulism in non-ruminants in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flaccid paralysis; Clostridium botulinum; Avian; Dogs; Horses; Pigs.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202158

Isolation and genotyping of Clostridium perfringens and Clostridium difficile in Capuchin Monkeys (Sapajus spp.)
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Ferreira,Débora Rochelly Alves; Laroquec,Plautino de Oliveira; Xavier,Rafael Gariglio Clark; Lobato,Francisco Carlos Faria; Mota,Rinaldo Aparecido.
ABSTRACT: The importance of Clostridium perfringens and C. difficile for most wild animal species remains unclear. This study aimed to isolate and genotype C. perfringens and C. difficile in stool samples from free-living and captive capuchin monkeys (Sapajus flavius and Sapajus libidinosus) in Brazil. Ten free-living S. flavius and 14 captive S. libidinosus were sampled for this study. To isolate C. difficile, stool samples were inoculated on plates containing cycloserine-cefoxitin fructose agar supplemented with horse blood and sodium taurocholate. Two different protocols for C. perfringens isolation were tested: direct plating onto selective agar and enrichment in brain heart infusion (BHI) broth followed by plating onto selective agar. C. difficile was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: New World Primates (NWP); Enterocolitis; Sapajus libidinosus; Sapajus flavius.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200452

Clostridium perfringens: a review of the disease in pigs, horses and broiler chickens
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Guedes,Roberto Maurício Carvalho; Lobato,Francisco Carlos Faria.
Clostridium perfringens is a gram-positive, anaerobic, fermentative, spore-forming bacillus that may be found in the environment but is more commonly found as part of the microbiota of humans and animals. The bacterium is considered a common enteric pathogen; however, the pathogenesis and predisposing factors of the disease can differ among species. Therefore, specific studies are necessary for understanding the role of this pathogen, how to diagnose it and which control measures are applicable. The aim of this paper is to review the current knowledge regarding C. perfringens infections in pigs, horses and broiler chickens
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Enteritis; Equine; Swine; Poultry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000601027

Botulismo em ruminantes causado pela ingestão de cama-de-frango
Provedor de dados: 65
Autores: Lobato,Francisco Carlos Faria; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Souza,Andréa Márcia de; Lima,Catarina Guimarães Rocha Dourado; Pires,Prhiscylla Sadanã; Assis,Ronnie Antunes de; Azevedo,Edisio Oliveira de.
Botulismo é uma intoxicação causada pela ingestão das toxinas produzidas pelo Clostridium botulinum, que acomete mamíferos e aves. Neste trabalho é descrito um surto de botulismo em ruminantes, ocorrido em duas propriedades localizadas no município de Patos, no Estado da Paraíba, Brasil. Em uma das propriedades, de um total de 88 bovinos, 85 (96,6%) vieram a óbito. Na segunda, morreram 145 ovinos (96,7%), 233 caprinos (57,8%) e 30 bovinos (96,8%). Os animais acometidos apresentavam paralisia progressiva, dificuldade de locomoção, sialorréia e dispnéia. A morte ocorreu entre 24 e 48 horas após o início dos sinais, por parada cardio-respiratória. Nenhuma alteração significativa foi observada no exame post-mortem. O diagnóstico de botulismo foi confirmado...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Botulismo; Clostridium botulinum; Cama-de-frango; Ruminantes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000400046

Salmonella Typhimurium - associated meningoencephalomyelitis in a foal
Provedor de dados: 65
Autores: Oliveira,Jéssica Guerra de; Ramos,Carolina Pantuzza; Rocha,Izabela de Assis; Marcelino,Sóstenes Apolo Correia; Pierezan,Felipe; Palhares,Maristela Silveira; Maranhão,Renata de Pino Albuquerque; Silva,Rodrigo Otávio Silveira; Teixeira,Raffaella Bertoni Cavalcanti.
ABSTRACT: A 10-day old foal presented with a history of acute recumbency and generalized weakness, that progressed to seizure episodes and death. Post mortem examination revealed necrotizing and purulent omphalophlebitis and fibrinopurulent meningoencephalomyelitis. Salmonella Typhimurium was isolated from the central nervous system and determined to be the cause of the meningoencephalomyelitis. Due to the lack of evidence of gastrointestinal disease, the umbilical cord was considered the most likely portal of entry of the bacteria. The isolated S. Typhimurium was resistant to ampicillin and cephalotin, and partially resistant to enrofloxacin. These drugs are commonly used in the treatment of salmonellosis. This is the first report of S. Typhimurium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; Salmonellosis; Omphalophlebitis; Meningoencephalitis; Diarrhea.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800451

Intra-abdominal granulomas caused by Clostridium tertium in an American Fuzzy Lop rabbit
Provedor de dados: 65
Autores: D’Elia,Mirella Lauria; Santos,Alice Barroso; Ribeiro,Beatriz Novaes Telles; Torres,Renato Cesar Sacchetto; Santos,Renato de Lima; Silva,Rodrigo Otávio Silveira; Nepomuceno,Anelise Carvalho.
ABSTRACT: A 6-year-old Fuzzy Lop rabbit was referred to a veterinary hospital with a complaint of lameness. In addition to a vertebral subluxation, two radiopaque and well-defined structures were revealed by radiographic evaluation. Ultrasonographically, the masses were characterized as parenchymal structures with diffuse mineralization and formation of reverberation artifacts, suggesting presence of gas. These two structures were excised during a laparotomy and Clostridium tertium was isolated. To the best of our knowledge, this is the first report of C. tertium infection in a pet animal.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridia; Granuloma; Diagnostic imaging.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100551

Padronização da titulação da toxina épsilon de Clostridium perfringens tipo D em linhagem contínua de células como alternativa ao bioensaio animal
Provedor de dados: 65
Autores: Souza Júnior,Milton Formiga; Lobato,Zélia Inês Portela; Pires,Prhiscylla Sadanã; Silva,Rodrigo Otávio Silveira; Salvarani,Felipe Masiero; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria.
Enterotoxemia, também chamada de doença do rim pulposo, doença que acomete os ruminantes domésticos, é causada pela ação da toxina épsilon produzida pelo Clostridium perfringens tipo D, um anaeróbio comumente isolado do solo e das fezes de animais sadios. O método tradicional de diagnóstico baseia-se na detecção e classificação dessa exotoxina no conteúdo intestinal por meio da soroneutralização em camundongos. Com isso, o objetivo deste estudo foi padronizar um teste para detecção e titulação dessa toxina in vitro e compará-lo ao fenômeno in vivo. Para isso, uma partida de toxina épsilon de Clostridium perfringens tipo D foi titulada em camundongos e em várias linhagens contínuas de células. Após a determinação da linhagem celular mais sensível,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterotoxemia; Bioética; Soroneutralização; MDCK.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000300016

Synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasis
Provedor de dados: 65
Autores: Rennó,Mário César; Vasconcellos,Amanda Otoni; Santos,Rogerio Rodrigues; Silva,Rodrigo Otávio Silveira; Carneiro,Rubens Antônio; Paes,Paulo Ricardo de Oliveira.
ABSTRACT:  Leishmaniasis represents a complex of chronic diseases with a broad geographic distribution and a high significance in public health worldwide. The varied clinical signs in conjunction with the low sensitivity and specificity of canine visceral leishmaniasis (CVL) detection methods make diagnosis of the disease complex. Among the several available laboratory tests, studies have suggested that the detection of parasites in synovial fluid (SF) is a good auxiliary tool in the diagnosis of CVL. However, no study has evaluated the influence of the clinical stage of CVL in the detection of Leishmania sp. in SF. This study aimed to evaluate the detection of Leishmania sp. amastigotes in the SF of dogs at different stages of the disease. The negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania infantum; Zoonosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500901

Padronização de um modelo de infecção por Clostridium difficile em hamsters sírios Mesocricetus auratus
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Guedes,Roberto Maurício Carvalho; Gabardo,Michelle de Paula; Oliveira Junior,Carlos Augusto; Salvarani,Felipe Masiero; Pires,Prhiscylla Sadanã; Alves,Guilherme Guerra; Lobato,Francisco Carlos Faria.
O objetivo do presente trabalho foi padronizar um modelo de infecção por Clostridium difficile (ICD) em hamsters sírios (Mesocricetus auratus). Para seleção dos isolados capazes de causar letalidade, cinco animais por grupo receberam uma dose de clindamicina (30mg kg-1) por gavagem. Após 48 horas, administraram-se 107 unidades formadoras de colônia (UFC), por animal, de quatro diferentes isolados toxigênicos de C. difficile. Selecinou-se um dos isolados capazes de causar diarreia e letalidade e administrou-se 4x102; 4x104; 4x106; 4x108UFC por animal, novamente com cinco hamsters por grupo. Em todas as diluições testadas, foi possível observar a ocorrência de diarreia e morte. A maior concentração testada (4x108UFC por animal) causou óbito de 100% dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium difficile; Diarreia nosocomial; Modelo animal; Infecção hospitalar.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000801415

Clostridium difficile infection: main features and occurrence in domestic species in Brazil
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Guedes,Roberto Maurício de Carvalho; Lobato,Francisco Carlos Faria.
Clostridium difficile is an emerging enteropathogen of humans and domestic animals. The bacterium was recently confirmed to be present in foals and dogs in Brazil, with some recent studies suggesting that C. difficile is one of the most important causes of piglet diarrhea in the country. Moreover, some reports also suggest the transmission of this microorganism between animals and humans, raising the possibility that C. difficile is a zoonotic disease. Therefore, the aim of the present review is to describe the main features of C. difficile infection in domestic animals and outline the occurrence of the disease in horses, dogs and pigs in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteritis; Zoonosis; Clostridiosis; Nosocomial; Piglets; Foals; Dogs.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100013

Botulismo tipo C em ganso ocorrido em Minas Gerais, Brasil
Provedor de dados: 65
Autores: Lobato,Francisco Carlos Faria; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Martins,Nelson Rodrigo Souza; Souza,Andréa Márcia de; Carvalho Filho,Maurício Baltazar de; Nascimento,Ricardo Aurélio Pinto; Assis,Ronnie Antunes de.
Botulismo é uma intoxicação causada pela ingestão das toxinas produzidas pelo Clostridium botulinum, que acomete mamíferos e aves, caracterizando-se por um quadro de paralisia flácida. Neste trabalho, é descrito um caso de botulismo em ganso, ocorrido no município de Santa Luzia, região metropolitana de Belo Horizonte, no Estado de Minas Gerais. Ao exame clínico, o animal apresentava-se com um quadro de paralisia flácida dos músculos do pescoço, das pernas e asas, além de apresentar ainda desprendimento de penas. A necropsia não revelou lesões significativas. Foi colhido o soro do animal e submetido ao teste de soroneutralização em camundongo, que identificou a toxina de C. botulinum tipo C.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Botulismo tipo C; Clostridium botulinum; Ganso; Aves.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000400047

Prevention of  Clostridium difficile infection in hamsters using a non-toxigenic strain
Provedor de dados: 65
Autores: Oliveira Júnior,Carlos Augusto de; Silva,Rodrigo Otávio Silveira; Diniz,Amanda Nádia; Pires,Prhiscylla Sadanã; Lobato,Francisco Carlos Faria; Assis,Ronie Antunes de.
ABSTRACT: The present study aimed to evaluate five non-toxigenic strains of Clostridium difficile (NTCD) in vitro and to select one strain to prevent C. difficile (CDI) infection in hamsters ( Mesocricetus auratus ). The NTCD strains were evaluated for spore production in vitro, antimicrobial susceptibility and presence of antimicrobial resistance genes. Approximately 107 spores of the selected strain (Z31) were administered by esophageal gavage in hamsters pretreated with 30mg kg-1 of clindamycin. The challenge with a toxigenic strain of C. difficile was conducted at 36 and 72h, and the animals were observed for 28 days. The NTCD strain of C. difficile (Z31) was able to prevent CDI in all animals that received it.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Clostridium difficile- associated diseases; Colitis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500853

Isolation and genotyping of Clostridium perfringens from goats in Minas Gerais, Brazil
Provedor de dados: 65
Autores: Santana,Jordana Almeida; Ferreira,Ana Carolina de Andrade; Souza,Marina de Castro Campos de; Moreira,Maria Aparecida Scatamburlo; Lima,Magna Coroa; Cruz,Diogo Soares Gonçalves; Lobato,Francisco Carlos Faria; Silva,Rodrigo Otávio Silveira.
ABSTRACT: Despite the known importance of Clostridium perfringens as an enteropathogen in small ruminants, little is known about the role of its additional virulence factors or the frequency of the various C. perfringens genotypes in healthy goats; this complicates the laboratory diagnosis of the infections caused by this microorganism. In light of this, the aim of the present study was to isolate and genotype C. perfringens from stool samples from healthy goats in Brazil. Stool samples from 250 apparently healthy adult goats from 17 different herds in Minas Gerais, Brazil were collected, and isolation and genotyping of C. perfringens was performed. C. perfringens type A was isolated from 189 (75.6%) goats, whereas C. perfringens types C and D were each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens type D; Microbiota; Epsilon; Beta.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700452

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil
Provedor de dados: 65
Autores: Coura,Fernanda Morcatti; Diniz,Amanda Nadia; Oliveira Junior,Carlos Augusto; Lage,Andrey Pereira; Lobato,Francisco Carlos Faria; Heinemann,Marcos Bryan; Silva,Rodrigo Otávio Silveira.
ABSTRACT: This study identified the virulence genes, pathovars, and phylogenetic groups of Escherichia coli strains obtained from the feces of dogs with and without diarrhea. Virulence genes and phylogenetic group identification were studied using polymerase chain reaction. Thirty-seven E. coli isolates were positive for at least one virulence factor gene. Twenty-one (57.8%) of the positive isolates were isolated from diarrheal feces and sixteen (43.2%) were from the feces of non-diarrheic dogs. Enteropathogenic E. coli (EPEC) were the most frequently (62.2%) detected pathovar in dog feces and were mainly from phylogroup B1 and E. Necrotoxigenic E. coli were detected in 16.2% of the virulence-positive isolates and these contained the cytotoxic necrotizing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dogs; Escherichia coli; Pathovars; Phylogroups.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200452

Genotyping and antimicrobial susceptibility of Clostridium perfringens isolated from Tinamidae, Cracidae and Ramphastidae species in Brazil
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Ferreira Junior,Francisco Carlos; Marques,Marcus Vinícius Romero; Oliveira Junior,Carlos Augusto; Martins,Nelson Rodrigo da Silva; Lobato,Francisco Carlos Faria.
The aim of this study was to isolate, genotype and evaluate the antimicrobial susceptibility of Clostridium perfringens found in species Tinamidae, Cracidae and Ramphastidae in Brazil. C. perfringens was isolated in 13 (5%) out of 260 swab samples and five (8.3%) out of 60 stool samples. All strains were classified as C. perfringens type A, and nine (50%) were positive for the beta-2 toxin-encoding gene. No strains were positive for the necrotic enteritis toxin B-like (NetB)-encoding gene. All isolates were susceptible to penicillin, metronidazole and vancomycin, whereas four (22.2%), five (27.8%) and 13 (72.2%) strains were considered resistant to erythromycin, oxytetracycline and lincomycin, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Necrotic enteritis; Avian; Toucan.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000300016