Consulta: creatorFacets:"Singh,Satparkash"
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Data/hora: 13/06/2024 04:18:43

Immune response to dna vaccine expressing transferrin binding protein a gene of Pasteurella multocida
Provedor de dados: 58
Autores: Singh,Satparkash; Singh,Vijendra Pal; Cheema,Pawanjit Singh; Sandey,Maninder; Ranjan,Rajeev; Gupta,Santosh Kumar; Sharma,Bhaskar.
Haemorrhagic Septicaemia (HS), an acute and fatal disease of cattle and buffalo is primarily caused by serotype B:2 or E:2 of Pasteurella multocida. The transferrin binding protein A (TbpA) has been found to act as immunogen and potent vaccine candidate in various Gram negative bacteria including P. multocida. The present study was carried out to evaluate the potential of this antigen as a DNA vaccine against HS in mice model. The tbpA gene of P. multocida serotype B:2 was cloned in a mammalian expression vector alone and along with murine IL2 gene as immunological adjuvant to produce monocistronic and bicistronic DNA vaccine constructs, respectively. The immune response to DNA vaccines was evaluated based on serum antibody titres and lymphocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA vaccine; Haemorrhagic septicaemia; IL2; Pasteurella multocida; Transferrin binding protein.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200043

Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples
Provedor de dados: 58
Autores: Singh,Harkanwaldeep; Rathore,R.S; Singh,Satparkash; Cheema,Pawanjit Singh.
In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected 143 faecal samples comprising human diarrheic stools (43), cattle diarrheic faeces (48) and poultry faecal swabs (52) only 4, 3 and 8,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter jejuni; Isolation; PCR; Spiking.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100022