Consulta: creatorFacets:"Stockdale, Ruth"
Registros recuperados: 4
Data/hora: 11/06/2024 11:12:54

Characterization of ten microsatellite loci in the blue mussel Mytilus edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy R.
Mussels of the genus Mytilus are ecologically and commercially important worldwide, and they form hybrid complexes where their distributions overlap. Allozyme and nuclear markers have bee It Used to investigate their genetics over many years, but successful development of reliable highly valuable microsatellite markers has lagged be hind other shellfish species. We have developed and characterized tell novel microsatellite loci that amplify reliably for the blue mussel Mytilus edulis. The number of alleles among 30 individuals from a wild population (Menai Strait, North Wales UK) ranged between 9 and 29 and the observed heterozygosity between 0.300 and 0.954. Significant heterozygote deficiencies against the Hardy-Weinberg model were observed at 6 out of...
Tipo: Text
Palavras-chave: Mytilus edulis; Microsatellites; Universal tailed-primer labeling; Null alleles; Mussels.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00002/11275/7841.pdf

Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Beaumont, Andy R.; Lapegue, Sylvie.
The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
Tipo: Text
Palavras-chave: Universal tailed primer labelling; Oysters; Ostrea edulis; Microsatellites.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00341/45195/44592.pdf

Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Beaumont, Andy R.; Lapegue, Sylvie.
The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
Tipo: Text
Palavras-chave: Universal tailed primer labelling; Oysters; Ostrea edulis; Microsatellites.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6421.pdf

Microsatellites development in Ostrea edulis and Mytilus edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Lapegue, Sylvie; Boudry, Pierre; Beaumont, Andy.
The European flat oyster Ostrea edulis and the blue mussel Mytilus edulis are both very valuable commercial species across Europe. Despite their economical importance, only a few microsatellite markers have so far been developed in those species. The aim of this study was to develop new microsatellites for O. edulis and M. edulis that could be used for population genetics studies, genetic variability assessment of stocks, parentage analysis or genetic and QTL mapping studies. For Ostrea edulis, an enriched library was made by ecogenics GmbH (Zurich, Switzerland) from size selected genomic DNA ligated into SAULA/SAULB-linker and enriched by magnetic bead selection with biotin-labelled (GT)13 and (CT)13 oligonucleotide repeats (Gautschi et al. 2000a,b). Of...
Tipo: Text
Palavras-chave: M13 tail; Blue mussel; European flat oyster; Microsatellite.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4536.pdf