Consulta: creatorFacets:"Tagawa, Michihito"
Registros recuperados: 7
Data/hora: 11/06/2024 14:31:08

Gene and protein expression of a soluble form of CTLA-4 in a healthy dog
Provedor de dados: 19
Autores: Tagawa, Michihito; Yamamoto, Yuhei; Shimbo, Genya; Iguchi, Aiko; Xuan, Xuenan; Tomihari, Mizuki; Miyahara, Kazuro.
Cytotoxic T lymphocyte associated gene-4 (CTLA-4) is a costimulatory molecule, expressed on the surface of activated T cells that negatively regulates T cell activation. In humans, alternative splicing of the CTLA-4 gene generates two major isoforms of mRNA, and a soluble form of CTLA-4 (sCTLA-4) was detected in normal human serum. We describe alternatively spliced mRNA expressed in peripheral blood mononuclear cells obtained from a healthy dog lacking the transmembrane domain coded by exon 3 of the CTLA-4 gene. Immunoprecipitation and western blotting of dog serum revealed a band of approximately 23-kDa, which is consistent with the predicted size, based on the amino acid sequence of the canine sCTLA-4 obtained in this study.
Palavras-chave: CD152; CTLA-4; Dog; Soluble form; Splicing variant.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4517

Enhancement of abdominal wall defect repair using allogenic platelet-rich plasma with commercial polyester/cotton fabric (Damour) in a canine model
Provedor de dados: 19
Autores: Abouelnasr, Khaled; Hamed, Mohamed; Lashen, Samah; El-Adl, Mohamed; Rasha, Eltaysh; Tagawa, Michihito.
Platelet-rich plasma (PRP) has an important role in musculoskeletal surgery; however, it has been underutilized for accelerating the healing of abdominal wall defects in veterinary practice. Therefore, the aim of this study was to evaluate the use of commercial polyester/cotton fabric (Damour) as a new composite mesh for the repair of experimentally induced abdominal wall defects in canine models, and to investigate the possible role of PRP for improving such repair and reducing allied complications. For this purpose, abdominal wall defects were created in 24 healthy mongrel dogs and then repaired with mesh alone (control group) or mesh and allogenic PRP (PRP group). Dogs were euthanized after 2 or 4 months for gross examination of implantation site,...
Palavras-chave: Abdominal wall defect; Damour; Dog; Platelet-rich plasma.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4542

Specific Molecular Detection of Anaplasma sp. Closely Related to Anaplasma phagocytophilum in Ixodid Ticks and Cattle in a Pastureland in Hokkaido, Japan
Provedor de dados: 19
Autores: Ybañez, Adrian P.; Tagawa, Michihito; Matsumoto, Kotaro; Kishimoto, Toshio; Yokoyama, Naoaki; Inokuma, Hisashi; 横山, 直明; 猪熊, 壽.
http://online.liebertpub.com/doi/abs/10.1089/vbz.2012.0963
Palavras-chave: Anaplasma sp.; Anaplasma phagocytophilum; Heat-shock operon; Ticks; Cattle.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3834

Fibrinous pericarditis secondary to bacterial infection in a cat
Provedor de dados: 19
Autores: Tagawa, Michihito; Kurashima, Chihiro; Shimbo, Genya; Omura, Hiroshi; Koyama, Kenji; Horiuchi, Noriyuki; Kobayashi, Yoshiyasu; Kawamoto, Keiko; Miyahara, Kazuro.
A three-year-old spayed domestic short-haired cat presented for evaluation of weight loss, cardiomegaly and pleural effusion. Echocardiographic examination demonstrated a thickened pericardium with mild pericardial effusion and a large volume of pleural effusion characterized by exudate. Although the cat was treated with antibiotics, the clinical symptoms did not improve. The cat developed dyspnea and died on day 7. Necropsy revealed a large amount of modified transudates ascites, pleural effusion and markedly dilated pericardium. Histopathological examination revealed severe exudation of fibrin and granulation tissue in a thick layer of the epicardium. The cat was diagnosed with fibrinous pericarditis secondary to bacterial infection.
Palavras-chave: Cardiomegaly; Cat; Fibrinous pericarditis; Moraxella osloensis.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4518

Ultrasonographic evaluation of depth width ratio (D/W) of benign and malignant mammary tumors in dogs
Provedor de dados: 19
Autores: Tagawa, Michihito; Kanai, Eiichi; Shimbo, Genya; Kano, Mikiya; Kayanuma, Hideki.
Depth-width ratio (D/W) is the only quantitative item in the criteria recommended by the Japanese Ultrasound Society for the evaluation of breast tumors in humans. However, the usefulness of the D/W has not been evaluated in dogs. Eighty-six mammary masses in 34 female dogs underwent ultrasonographic examination to determine the D/W and other characteristics. Results of ultrasonographic and histopathologic examinations were compared. The D/W of malignant tumors was significantly greater than that of benign tumors, and it had a sensitivity of 56.3% and a specificity of 92.9% for the diagnosis of malignancy when the threshold of D/W was 0.7. In addition, irregular margin, polymorphous shape and heterogeneous internal echographic characteristics were...
Palavras-chave: Canine; Depth width ratio; Mammary tumor; Ultrasound.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4456

Evaluation of Costimulatory Molecules in Peripheral Blood Lymphocytes of Canine Patients with Histiocytic Sarcoma
Provedor de dados: 19
Autores: Tagawa, Michihito; Maekawa, Naoya; Konnai, Satoru; Takagi, Satoshi.
Histiocytic sarcoma is a rapidly progressive and fatal neoplastic disease in dogs. It is unclear whether costimulatory molecules, including CD28, cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4), and programmed death-1 (PD-1), are expressed on peripheral blood lymphocytes (PBLs) of canine patients with histiocytic sarcoma. The objective of this study was to evaluate the expression of CD28, CTLA-4, and PD-1 molecules on PBLs of patients with histiocytic sarcoma, patients with other tumors, and healthy controls. Twenty-six dogs were included in the study, with eight, ten, and eight dogs in the histiocytic sarcoma, other tumor, and healthy control groups, respectively. PBLs and serum were prospectively obtained from patients diagnosed histopathologically...
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4455

Lissencephaly in a Pekingese
Provedor de dados: 19
Autores: Shimbo, Genya; Tagawa, Michihito; Oohashi, Eiji; Yanagawa, Masashi; Miyahara, Kazuro.
A 1-year-old neutered male Pekingese was presented for evaluation and further treatment of cluster seizures. The dog had behavioral abnormalities, and a prosencephalic lesion was suspected following neurological examination. The dog showed signs of learning difficulty. Magnetic resonance imaging of the brain revealed a remarkably smooth cerebral cortex with a reduced number of gyri, as well as a cystic lesion associated with the quadrigeminal cistern. A diagnosis of lissencephaly, concurrent with a quadrigeminal cisternal cyst, was made. High-dose and multiple anticonvulsants were necessary to control the seizures. This is the first report of lissencephaly in a Pekingese. © 2017 The Japanese Society of Veterinary Science.
Palavras-chave: Lissencephaly; Magnetic resonance imaging; Pekingese.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4564