Consulta: creatorFacets:"Takabatake, Noriyuki"
Registros recuperados: 5
Data/hora: 02/06/2024 12:38:04

TRANSIENT EXPRESSION OF A GREEN FLUORESCENT PROTEIN GENE IN BABESIA BOVIS
Provedor de dados: 19
Autores: Fujii, Kei; Yokoyama, Naoaki; Takabatake, Noriyuki; Suzuki, Hiroshi; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 鈴木, 宏志; 玄, 学南; 五十嵐, 郁男.
In hemoprotozoa，gene transfer technology provides an important tool to aid in the functional study of parasite genes. In this study，a transfer vector containing the enhanced green fluorescent protein (EGFP) gene laid between the Toxoplasma gondii GRAI promoter region and the GRA2 polyA signal region was constructed and transfected into the in vitro culture of Babesia boνis by the electroporation method.On the first and second days post-transfection，clear positive fluorescences were detected in some parasite bodies， indicating the successful expression of the EGFP gene controlled by the GRA-1 promoter in B. bovis. However， the positive parasites were not ubiquitous and finally disappeared until the forth day post-transfection. This is the first time that...
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/631

IMMUNOSCREENING OF BABESIA RODHAINI GENES ENCODING IMMUNOGENIC PROTEINS FROM THE CDNA EXPRESSION PHAGE LIBRARY
Provedor de dados: 19
Autores: Fujii, Kei; Takabatake, Noriyuki; Boonchit, Suthisak; Hirata, Haruyuki; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
Palavras-chave: Babesia rodhaini; Babesiosis; Immunoscreening; CDNA expression library.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/626

FUCOIDAN INHIBITS THE IN VITRO GROWTH OF BABESIA BOVIS
Provedor de dados: 19
Autores: Takabatake, Noriyuki; Hashiba, Shintaro; Bork, Sabine; Okamura, Masashi; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
Palavras-chave: Babesia bovis; Fucoidan.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/646

Protective immunity of blood plasma fraction containing Babesia rodhaini exoantigens in mice
Provedor de dados: 19
Autores: Kamiya, Shiho; Takabatake, Noriyuki; Fujii, Kei; Okamura, Masashi; Iseki, Hiroshi; Yokoyama, Naoaki; Igarashi, Ikuo.
Babesia spp. are major hemoprotozoan parasites in animals. Exoantigens, which are soluble antigens released by the parasites during their asexual development, have been successfully applied for the development of a vaccine against babesiosis. In this study, we examined the ability of exoantigens to induce protective immunity in mice against a rodent Babesia parasite, Babesia rodhaini. The BALB/c mice immunized with the blood plasma fraction of B. rodhaini-infected mice (B. rodhaini exoantigens) showed a significant decrease in the lethal rate when challenged with B. rodhaini. Western blot analysis with anti-B. rodhaini exoantigen immune serum specifically detected at least eleven proteins in the lysate of B. rodhaini-infected erythrocytes. Next, a cDNA...
Palavras-chave: Babesiosis; Babesia rodhaini; Exoantigen; Protective effect; Mice.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/149

Babesia bovis: Subcellular localization of host erythrocyte membrane components during their asexual growth
Provedor de dados: 19
Autores: Okamura, Masashi; Yokoyama, Naoaki; Takabatake, Noriyuki; Okubo, Kazuhiro; Ikehara, Yuzuru; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
In the present study, the subcellular localization of the host red blood cell (RBC) membrane components, the alpha 2-3-linked sialic acid (SA) residues and the lipid bilayer, was observed during the asexual growth of Babesia bovis using two erythrocyte probes, the SA-specific lectin (MALII) and the lipophilic fluorescent (PKH2) probes, respectively. In confocal laser scanning microscopy with MALII, the SA residues on the surface of parasitized RBCs appeared to accumulate into the intracellular parasites as the parasites matured as well as to remain on the surface of extracellular parasites. Furthermore, when PKH2-labeled RBCs were infected with B. bovis, PKH2 signals were also observed around both the intracellular and the extracellular parasites,...
Palavras-chave: Babesia bovis (B. bouis); SA; MALII; RBC; RT; PBS; Babesia equi (B. equi); PV.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1040