Consulta: creatorFacets:"Takita,Marco Aur�lio"
Registros recuperados: 4
Data/hora: 14/06/2024 02:44:45

CitEST libraries
Provedor de dados: 74
Autores: Targon,Maria Luísa P. Natividade; Takita,Marco Aurélio; Amaral,Alexandre M. do; Souza,Alessandra A. de; Locali-Fabris,Eliane Cristina; Dorta,Sílvia de Oliveira; Borges,Kleber Martins; Souza,Juliana Mendonça de; Rodrigues,Carolina Munari; Lucheta,Adriano Reis; Freitas-Astúa,Juliana; Machado,Marcos Antonio.
In order to obtain a better understanding of what is citrus, 33 cDNA libraries were constructed from different citrus species and genera. Total RNA was extracted from fruits, leaves, flowers, bark, seeds and roots, and subjected or not to different biotic and abiotic stresses (pathogens and drought) and at several developmental stages. To identify putative promoter sequences, as well as molecular markers that could be useful for breeding programs, one shotgun library was prepared from sweet orange (Citrus sinensis var. Olimpia). In addition, EST libraries were also constructed for a citrus pathogen, the oomycete Phythophthora parasitica in either virulent or avirulent form. A total of 286,559 cDNA clones from citrus were sequenced from their 5’ end,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ESTs; Shotgun; Citrus; Phytopathogen.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500030

A genetic framework for flowering-time pathways in Citrus spp.
Provedor de dados: 74
Autores: Dornelas,Marcelo Carnier; Camargo,Raquel Luciana Boscariol; Figueiredo,Luciana Harumi Morimoto; Takita,Marco Aurélio.
Floral transition is one the most drastic changes occurring during the life cycle of a plant. The shoot apical meristem switches from the production of leaves with associated secondary shoot meristems to the production of flower meristems. This transition is abrupt and generally irreversible, suggesting it is regulated by a robust gene regulatory network capable of driving sharp transitions. The moment at which this transition occurs is precisely determined by environmental and endogenous signals. A large number of genes acting within these pathways have been cloned in model herbaceous plants such as Arabidopsis thaliana. In this paper, we report the results of our search in the Citrus expressed sequence tag (CitEST) database for expressed sequence tags...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproductive development; Flowering-time genes; Reproduction; Photoperiod; CONSTANS.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500006

Towards the identification of flower-specific genes in Citrus spp
Provedor de dados: 74
Autores: Dornelas,Marcelo Carnier; Camargo,Raquel Luciana Boscariol; Berger,Irving Joseph; Takita,Marco Aurélio.
Citrus sinensis is a perennial woody species, for which genetic approaches to the study of reproductive development are not readily amenable. Here, the usefulness of the CitEST Expressed Sequence Tag (EST) database is demonstrated as a reliable new resource for identifying novel genes exclusively related to Citrus reproductive biology. We performed the analysis of an EST dataset of the CitEST Project containing 4,330 flower-derived cDNA sequences. Relying on bioinformatics tools, sequences exclusively present in this flower-derived sequence collection were selected and used for the identification of Citrus putative flower-specific genes. Our analysis revealed several Citrus sequences showing significant similarity to conserved genes known to have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Development; Flower-specific genes; Reproduction; Transcription factors.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500005

Signaling pathways in a Citrus EST database
Provedor de dados: 74
Autores: Mehta,Angela; Silva,Marilia Santos; Guidetti-Gonzalez,Simone; Carrer,Helaine; Takita,Marco Aurélio; Martins,Natália F.
Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis) was launched in order to sequence Citrus ESTs (expressed sequence tags) from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under different development stages (adult vs. juvenile). Several cDNA libraries were constructed and the sequences obtained formed the Citrus ESTs database with almost 200,000 sequences. Searches were performed in the Citrus database to investigate the presence of different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Cell signaling; Genomics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500003