Consulta: creatorFacets:"Takiuchi,Elisabete"
Registros recuperados: 3
Data/hora: 18/06/2024 16:55:30

An outbreak of winter dysentery caused by bovine coronavirus in a high-production dairy cattle herd from a tropical country
Provedor de dados: 52
Autores: Takiuchi,Elisabete; Barry,Aline Fernandes; Alfieri,Alice Fernandes; Filippsen,Patrícia; Alfieri,Amauri Alcindo.
Bovine coronavirus (BCoV) is a known cause of winter dysentery (WD) in adult cattle. The morbidity of the disease is high, that results in a significant decrease in milk production and consequently, economic losses. In the present study, we report on a classical outbreak of WD that affected a high-production Holstein dairy herd raised in a tropical country. The lactating batch included 154 cows, and 138 (90%) presented diarrhea in a short (nine days) period of time. Three (2%) cows died. The other batches of animals did not become ill. The evolution of the disease in the herd, including the clinical signs and epidemiological features, strongly suggested a WD case. Semi-nested PCR and RFLP confirmed that BCoV was the cause of the infection. Samples tested...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Dairy herd; Winter dysentery; Diarrhea; Bovine coronavirus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700008

Avaliação da capacidade adjuvante do cloreto de dimetildioctadecilamônio associado ao hidróxido de alumínio na indução da resposta imune humoral de bovinos vacinados com o vírus da diarréia viral bovina
Provedor de dados: 62
Autores: Silva,Luis César da; Takiuchi,Elisabete; Médici,Kerlei Cristina; Alfieri,Alice Fernandes; Alfieri,Amauri Alcindo.
A resposta imunológica humoral de bovinos vacinados com o vírus da diarréia viral bovina (BVDV) inativado, tendo como adjuvante o cloreto de dimetildioctadecilamônio (DDA cloreto) associado ao hidróxido de alumínio (vacina B), foi comparada com uma vacina contendo o mesmo antígeno adsorvido apenas com hidróxido de alumínio (vacina A). Duas semanas após a segunda dose foi avaliado o título de anticorpos neutralizantes dos animais que receberam as duas preparações de antígenos. Os animais que receberam a vacina B apresentaram melhor resposta imune humoral quando comparados com os animais vacinados com a vacina A. O título médio de anticorpos neutralizantes, expresso em Log2, dos animais que receberam a vacina B foi superior (P&lt;0,05) ao observado no grupo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Vírus da diarréia viral bovina; Cloreto de dimetildioctadecilamônio; Vacina; Adjuvante.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000300009

Identification of a mutation in the spike protein cleavage site in Brazilian strains of wild-type bovine coronavirus
Provedor de dados: 58
Autores: Takiuchi,Elisabete; Barreiros,Marco Antônio Bacellar; Alfieri,Alice Fernandes; Alfieri,Amauri Alcindo.
The spike (S) protein of coronaviruses, a type I membrane glycoprotein, is primarily responsible for entry into susceptible cells by binding with specific receptors on cells and mediating subsequent virus-cell fusion. The bovine coronavirus (BCoV) S protein is cleaved into two subunits, the N-terminal S1 and the C-terminal S2. The proteolytic cleavage site of S protein is highly conserved among BCoV strains and is located between amino acids 763 and 768 (KRRSRR). This study describes a single mutation in the S protein cleavage site of three Brazilian strains of BCoV detected in diarrheic fecal samples from calves naturally infected. The sequenced PCR products revealed that amino acid sequence of the cleavage site of our strains was KRRSSR, indicating a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BCoV; Sequencing; Spike protein; Cleavage site; Mutation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400021