Consulta: creatorFacets:"Tamayo, David"
Registros recuperados: 6
Data/hora: 06/06/2024 08:25:49

Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection
Provedor de dados: 5
Autores: Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie.
Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters...
Tipo: Text
Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf

Effet de traitements thermiques sur la résistance du naissain d’huître creuse au phénomène de mortalité massive - THERMARES
Provedor de dados: 5
Autores: Pernet, Fabrice; Petton, Bruno; Tamayo, David.
L’objectif de cette étude vise à déterminer l’effet de basses températures (10°C et 13°C) pendant des durées variables (0 et 80 jours) sur la survie du naissain d’huîtres creuses préalablement infecté suite à une exposition en rade de Brest pendant 5 ou 8 jours.  Le naissain d’huître creuse préalablement infecté par OsHV-1 µvar par exposition en milieu naturel pendant 5 d ou 8 d, puis transféré à basse température (10°C ou 13°C) pendant 5 à 80 d, présente des mortalités plus faibles et plus tardives que les animaux témoins en laboratoire. La réduction des mortalités observée dans le naissain exposé à basse température coïncide avec une diminution progressive des concentrations d’ADN appartenant à OsHV-1 dans les tissus, au point de devenir non-détecté ou...
Tipo: Text
Palavras-chave: Huître creuse; Naissain; Mortalité massive; OsHV-1 μvar; Température; Pacific oyster; Mass mortality; OsHV-1 μvar; Spat; Temperature.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00210/32135/30592.pdf

Influence of low temperatures on the survival of the Pacific oyster (Crassostrea gigas) infected with Ostreid herpesvirus type 1
Provedor de dados: 5
Autores: Pernet, Fabrice; Tamayo, David; Petton, Bruno.
Mortalities of oyster seed of Crassostrea gigas associated with ostreid herpes virus OsHV-1 μVar have been observed in many oyster producing countries since 2008. The objective of this study was to further investigate whether low temperature treatments can offer a viable option to mitigate oyster mortalities. An experiment was set-up to further evaluate the effect of low temperature treatments (10 and 13°C vs. 21°C) and their duration (6 d to 83 d) on the survival of oysters previously infected with OsHV-1 μVar by means of exposure to field conditions in areas where mortalities were occurring. Average survival of oysters infected with OsHV-1μVar was 71% after 83 d at low temperatures compared to only 23% in controls maintained at 21°C. During...
Tipo: Text
Palavras-chave: Bivalve; Disease; OsHV-1; Oyster; Temperature; Virus.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00259/37047/35555.pdf

Physiological basis of extreme growth rate differences in the spat of oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Tamayo, David; Ibarrola, Irrintzi; Urrutxurtu, Inaki; Navarro, Enrique.
Juvenile oysters (Crassostrea gigas) (produced in November 2009) reared under uniform hatchery conditions for 4 months were selected for extreme growth rate differences by repeatedly taking larger and smaller individuals to achieve weight differences &gt; 30x between fast (F) and slow (S) growers. The physiological basis of differential growth was analyzed in experiments in June 2010, where components of energy gain (clearance and ingestion rates and absorption efficiency), energy loss (metabolic rates) and resulting scope for growth (J h(-1)) were compared for groups of F and S oysters fed three different ration levels (a parts per thousand 0.5, 1.5 and 3.0 mg of total particulate matter L-1). In both F and S oysters, a higher food ration promoted...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00201/31244/29909.pdf

Physiological changes in Pacific oyster Crassostrea gigas exposed to the herpesvirus OsHV-1 μvar
Provedor de dados: 5
Autores: Tamayo, David; Corporeau, Charlotte; Petton, Bruno; Quere, Claudie; Pernet, Fabrice.
Since 2008, mass mortality events of Crassostrea gigas have been occurring along the French coast when seawater temperature exceeds 16ºC. These mortality events are related to a particular genotype of the ostreid herpesvirus named OsHV-1 μvar. The present study aimed to detail various physiological aspects underlying the onset of the disease. In the laboratory, both exposed (infected) and naïve (healthy) oysters were maintained at 13.0°C and 20.6ºC. These temperatures were respectively lower and higher than the threshold values of 16°C at which the disease generally occur. At 20.6ºC, exposed oysters were characterized by a reduction in energetic reserves (carbohydrates and triglycerides) together with a decrease in protein content. Sterols levels were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Energetic reserves; Energetic metabolism; Enzyme activity; OsHV-1; Temperature.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00192/30330/28803.pdf

Deciphering the effect of food availability, growth and host condition on disease susceptibility in a marine invertebrate
Provedor de dados: 5
Autores: Pernet, Fabrice; Tamayo, David; Fuhrmann, Marine; Petton, Bruno.
Food provisioning influences disease risk and outcome in animal populations in two ways. On the one hand, unrestricted food supply improves the physiological condition of the host and lowers its susceptibility to infectious disease, reflecting a trade-off between immunity and other fitness-related functions. On the other hand, food scarcity limits the resources available to the pathogen and slows the growth and metabolism of the host on which the pathogen depends to proliferate. Here, we investigated how food availability, growth rate and energetic reserves drive the outcome of a viral disease affecting an ecologically relevant model host, the Pacific oyster, Crassostrea gigas. We selected fast- and slow-growing animals, and we exposed them to high and low...
Tipo: Text
Palavras-chave: Energetics; Health; Herpesvirus; Nutrition; Physiological trade-off.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00513/62431/66716.pdf