Consulta: creatorFacets:"Tanguy, Aurelie"
Registros recuperados: 2
Data/hora: 17/06/2024 02:30:22

Characterisation and expression of four mRNA sequences encoding glutathione S-transferases pi, mu, omega and sigma classes in the Pacific oyster Crassostrea gigas exposed to hydrocarbons and pesticides
Provedor de dados: 5
Autores: Boutet, Isabelle; Tanguy, Aurelie; Moraga, Dario.
Hydrocarbon and pesticide pollution in coastal ecosystems can disturb marine bivalve metabolism. In this study, we characterised four full-length cDNA sequences encoding glutathione S-transferases (GSTs) in the Pacific oyster Crassostrea gigas. A BLAST X search showed that these four sequences encode GSTs from four different classes: GST pi, sigma, mu and omega. A phylogenetic analysis of GST was made to determine the position of oyster GST compared to invertebrate and vertebrate sequences. We developed a semi-quantitative, multiplex RT-PCR to follow the expression of these four GSTs in tissues of oysters exposed to hydrocarbons and two pesticide treatments ( glyphosate and a mixture composed of atrazine, diuron and isoproturon) under experimental...
Tipo: Text
Palavras-chave: Crassostrea gigas; Gene expression; Multiplex RTPCR; Pesticides; Hydrocarbons; CDNA sequences; Glutathione S transferases.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-431.pdf

Molecular identification and expression of two non-P450 enzymes, monoamine oxidase A and flavin-containing monooxygenase 2, involved in phase I of xenobiotic biotransformation in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Boutet, Isabelle; Tanguy, Aurelie; Moraga, Dario.
Marine bivalve metabolism can be perturbed by hydrocarbon and pesticide pollution in coastal ecosystems. In this study, in the Pacific oyster, Crassostrea gigas, full-length cDNAs encoding two non-P450 phase I enzymes, flavin-containing monooxygenase 2 (FMO-2) and monamine oxidase A (MAO A), were characterized. Both sequences contained the co-factor fixation motifs characteristic of their respective enzyme families. Using reverse transcription polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) transcription levels of these two enzymes in tissues of oysters exposed, under experimental conditions, to hydrocarbons and two pesticide treatments were investigated. The pesticide treatments were exposure to either glyphosate or to a mixture composed of...
Tipo: Text
Palavras-chave: Crassostrea gigas; Flavin containing monooxygenase 2; Monoamine oxidase A.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-676.pdf