Consulta: creatorFacets:"Tap, Julien"
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Comparative assessment of human and farm animal faecal microbiota using real-time quantitative PCR
Provedor de dados: 5
Autores: Furet, Jean-pierre; Firmesse, Olivier; Gourmelon, Michele; Bridonneau, Chantal; Tap, Julien; Mondot, Stanislas; Dore, Joel; Corthier, Gerard.
Pollution of the environment by human and animal faecal pollution affects the safety of shellfish, drinking water and recreational beaches. To pinpoint the origin of contaminations, it is essential to define the differences between human microbiota and that of farm animals. A strategy based on real-time quantitative PCR (qPCR) assays was therefore developed and applied to compare the composition of intestinal microbiota of these two groups. Primers were designed to quantify the 16S rRNA gene from dominant and subdominant bacterial groups. TaqMan((R)) probes were defined for the qPCR technique used for dominant microbiota. Human faecal microbiota was compared with that of farm animals using faecal samples collected from rabbits, goats, horses, pigs, sheep...
Tipo: Text
Palavras-chave: Quantitative PCR; Faecal microbiota; Human; Farm animals.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00000/11071/11297.pdf