Consulta: creatorFacets:"Terkawi, Mohamad Alaa"
Registros recuperados: 8
Data/hora: 11/06/2024 08:08:35

Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay
Provedor de dados: 19
Autores: Sandagdorj, Narantsatsral; Goo, Youn-Kyoung; Terkawi, Mohamad Alaa; Soma, Takehisa; Luo, Yuzi; Li, Yan; Cao, Shinuo; Aboge, Gabriel Oluga; Nishikawa, Yoshifumi; Badgar, Battsetseg; Xuan, Xuenan.
Among the previously established enzyme-linked immunosorbent assays (ELISAs), an ELISA using the full length of recombinant thrombospondin-related adhesive protein of Babesia gibsoni (rBgTRAPf) is considered as the most sensitive diagnostic method for the detection of antibody to B. gibsoni in dogs. However, the expression of rBgTRAPf in high concentration is poor and thus limits its usefulness as diagnostic antigen. To improve its expression level, we have truncated BgTRAPf into two fragments having either N- or C-terminus (BgTRAPn or BgTRAPc). The expression of BgTRAPc protein in Escherichia coli yielded adequate recombinant protein. The specificity and sensitivity of ELISAs with the truncated proteins were determined using B. gibsoni-experimentally...
Palavras-chave: Babesia gibsoni; Diagnosis; Enzyme-linked immunosorbent assay (ELISA); Thrombospondin-related adhesive protein (TRAP).
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3115

Depletion of Phagocytic Cells during Nonlethal Plasmodium yoelii Infection Causes Severe Malaria Characterized by Acute Renal Failure in Mice
Provedor de dados: 19
Autores: Terkawi, Mohamad Alaa; Nishimura, Maki; Furuoka, Hidefumi.
In the current study, we examined the effects of depletion of phagocytes on the progression of Plasmodium yoelii 17XNL infection in mice. Strikingly, the depletion of phagocytic cells, including macrophages, with clodronate in the acute phase of infection significantly reduced peripheral parasitemia but increased mortality. Moribund mice displayed severe pathological damage, including coagulative necrosis in liver and thrombi in the glomeruli, fibrin deposition, and tubular necrosis in kidney. The severity of infection was coincident with the increased sequestration of parasitized erythrocytes, the systematic upregulation of inflammation and coagulation, and the disruption of endothelial integrity in the liver and kidney. Aspirin was administered to the...
Palavras-chave: Phagocytes; Macrophages; Phagocytosis; Plasmodium yoelii.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4479

Molecular Prevalence of Different Genotypes of Theileria orientalis Detected from Cattle and Water Buffaloes In Thailand
Provedor de dados: 19
Autores: Altangerel, Khukhuu; Sivakumar, Thillaiampalam; Inpankaew, Tawin; Jittapalapong, Sathaporn; Terkawi, Mohamad Alaa; Ueno, Akio; Xuan, Xuenan; Igarashi, Ikuo; Yokoyama, Naoaki; 玄, 学南; 五十嵐, 郁男; 横山, 直明.
Here we report on an epidemiological study regarding the molecular prevalence of different genotypes of Theileria orientalis present among domestic cattle and water buffalo populations bred in Thailand. A phylogenetic analysis based on the parasitic gene encoding a major piroplasm surface protein revealed the presence of 5 genotypes (Types 1, 3, 5, 7, and N-3) in cattle and 7 genotypes (Types 1, 3, 4, 5, 7, N-2, and N-3) in water buffaloes. Types 4, 7, and N-3 of T. orientalis were reported for the first time in water buffaloes. The previously reported C and Thai types from Thailand clustered as types 7 and 6, respectively, in the present analysis. Great similarities were observed among nucleotide sequences of isolates of the same genotype from cattle and...
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3592

Dextran sulfate inhibits acute Toxoplama gondii infection in pigs
Provedor de dados: 19
Autores: Kato, Kentaro; Murata, Yuho; Horiuchi, Noriyuki; Inomata, Atsuko; Terkawi, Mohamad Alaa; Ishiwa, Akiko; Ogawa, Yohsuke; Fukumoto, Shinya; Matsuhisa, Fumikazu; Koyama, Kenji.
Background: Toxoplasma gondii is a highly prevalent protozoan that can infect all warm-blooded animals, including humans. Its definitive hosts are Felidae and its intermediate hosts include various other mammals and birds, including pigs. It is found in the meat of livestock which is a major source of human infection. Hence the control of toxoplasmosis in pigs is important for public health. We previously showed that dextran sulfate (DS), especially DS10 (dextran sulfate MW 10 kDa), is effective against T. gondii infection both in vitro and in mice. In this study, we asked whether DS affects T. gondii infection of pigs, one of the main animal sources of toxoplasmosis transmission to humans. Methods: Fourteen-day-old male pigs (n = 10) were infected with T....
Palavras-chave: Dextran sulfate; Intermediate host; Pig; Public health; Toxoplasma gondii.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4446

Involvement of beta-defensin 130 (DEFB130) in the macrophage microbicidal mechanisms for killing Plasmodium falciparum
Provedor de dados: 19
Autores: Terkawi, Mohamad Alaa; Takano, Ryo; Furukawa, Atsushi; Murakoshi, Fumi; Kato, Kentaro.
Understanding the molecular defense mechanism of macrophages and identifying their effector molecules against malarial parasites may provide important clues for the discovery of new therapies. To analyze the immunological responses of malarial parasite-induced macrophages, we used DNA microarray technology to examine the gene profile of differentiated macrophages phagocytizing Plasmodium falciparum-parasitized erythrocytes (iRBC). The transcriptional gene profile of macrophages in response to iRBCs represented 168 down-regulated genes, which were mainly involved in the cellular immune response, and 216 upregulated genes, which were involved in cellular proteolysis, growth, and adhesion. Importantly, the specific upregulation of beta-defensin 130 (DEFB130)...
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4448

Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1)
Provedor de dados: 19
Autores: Zhou, Mo; Cao, Shinuo; Luo, Yuzi; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Iguchi, Aiko; Vudriko, Patrick; Terkawi, Mohamad Alaa; Lowenstein, Mario; Kern, Angela; Nishikawa, Yoshifumi; Suzuki, Hiroshi; Igarashi, Ikuo; Xuan, Xuenan.
Background: Babesia canis is an apicomplexan tick-transmitted hemoprotozoan responsible for causing canine babesiosis in Europe and west Asia. Despite its importance, there is no known rapid diagnostic kit detection of B. canis infection in dogs. The present study identified two novel antigens of B. canis and used the recombinant antigens to establish a rapid, specific and sensitive serodiagnostic technique for detection of B. canis infection. Methods: A complementary DNA (cDNA) expression library was constructed from the mRNA of B. canis and immunoscreened using B. canis-infected dog sera. The cDNAs encoding a merozoite surface antigen and a secreted antigen protein were identified and designated as BcMSA1 and BcSA1, respectively. The recombinant BcMSA1...
Palavras-chave: Babesia canis; Canine babesiosis; BcMSA1; BcSA1; ELISA; Immunochromatographic tests.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4420

Serodiagnosis of ovine toxoplasmosis in Mongolia by an enzyme-linked immunosorbent assay with recombinant Toxoplasma gondii matrix antigen 1
Provedor de dados: 19
Autores: Tumurjav, Buyannemekh; Terkawi, Mohamad Alaa; Zhang, Houshuang; Zhang, Guohong; Jia, Honglin; Goo, Youn-Kyoung; Yamagishi, Junya; Nishikawa, Yoshifumi; Igarashi, Ikuo; Sugimoto, Chihiro; Xuan, Xuenan.
Toxoplasma gondii matrix antigen 1 (TgMAG1), known as the 65-kDa protein, which is abundantly expressed in both bradyzoites and tachyzoites, was evaluated as a candidate for the development of a diagnostic reagent for ovine toxoplasmosis. The TgMAG1 gene was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST),and the recombinant TgMAG1 (rTgMAG1) was tested in an enzyme-linked immunosorbent assay (ELISA). The ELISA with rTgMAG1 showed a highly specific reaction with sera from mice experimentally infected with T. gondii but not with the closely related Neospora caninum. The antibodies to TgMAG1 were detectable from the acute to the chronic infectious stages in a mouse model. A total of 175 serum samples collected from sheep...
Palavras-chave: ELISA; Mongolia; Sheep; TgMAG1; Toxoplasma gondii.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3018

Immunization with Toxoplasma gondii peroxiredoxin 1 induces protective immunity against toxoplasmosis in mice
Provedor de dados: 19
Autores: Fereig, Ragab M.; Kuroda, Yasuhiro; Terkawi, Mohamad Alaa; Mahmoud, Motamed Elsayed; Nishikawa, Yoshifumi.
To develop a vaccine against Toxoplasma gondii, a vaccine antigen with immune-stimulating activity is required. In the present study, we investigated the immunogenicity and prophylactic potential of T. gondii peroxiredoxin 1 (TgPrx1). The TgPrx1 was detected in the ascitic fluid of mice 6 days postinfection, while specific antibody levels were low in the sera of chronically infected mice. Treatment of murine peritoneal macrophages with recombinant TgPrx1 triggered IL-12p40 and IL-6 production, but not IL-10 production. In response to TgPrx1, activation of NF-kappa B and IL-6 production were confirmed in mouse macrophage cell line (RAW 264.7). These results suggest the immune-stimulating potentials of TgPrx1. Immunization of mice with recombinant TgPrx1...
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4462