Consulta: creatorFacets:"Thomas Guyon, Hélène"
Registros recuperados: 2
Data/hora: 11/06/2024 11:12:20

A flow cytometric approach to study intracellular-free Ca2+ in Crassostrea gigas haemocytes
Provedor de dados: 5
Autores: Aton, Emilie; Renault, Tristan; Gagnaire, Beatrice; Thomas Guyon, Hélène; Cognard, C; Imbert, N.
Bivalve haemocytes are essential in defence mechanisms including phagocytosis. They also produce molecules including hydrolytic enzymes and antimicrobial peptides that contribute to pathogen destruction. Although haemocyte activities have been extensively studied, relatively little is known about the intracellular signalling pathways that are evoked during haemocyte activation and especially the role of calcium.   Flow cytometry has been used for the first time to define the effect of cell incubation in haemolymph and artificial sea water (ASW) on Pacific oyster, Crassostrea gigas, haemocytes. Cell viability, enzymatic activities (esterases and amino peptidases), phagocytosis and granulocyte percentage were analysed. Viability and some activities were...
Tipo: Text
Palavras-chave: Cellular activities; Fluo 3/AM; Calcium; Flow cytometry; Haemocytes; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-935.pdf

Effect of in vivo pesticide exposure and injection of bacteria on immune gene expression in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Renault, Tristan; Thomas Guyon, Hélène; Burgeot, Thierry.
Bay of Marennes-Oleron is an important French area of reproduction for sea bass, Dicentrarchus labrax. This area is often subjected to many pollutions due to anthropic activities, including pesticides and polycyclic aromatic hydrocarbons (PAH) which cause adverse effects in aquatic organisms. The harmful effects of such   contaminants on fish immune system leading to a decreased resistance against pathogens begin to be described, but remain poorly  known. In this context, influence of micropolluants, like PAH, on the defense mechanisms developed by sea bass was tested. Fish were exposed five days to a soluble fraction of PAH with environmental concentration (760 ng/L). At the end of the contamination, fish were transferred to non contaminated sea water and...
Tipo: Text
Palavras-chave: Phagocytosis; Vibrio; Pesticides; Pollutant; Crasssotrea gigas; Oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3317.pdf