Consulta: creatorFacets:"Thorne, Michael A. S."
Registros recuperados: 4
Data/hora: 11/06/2024 12:29:34

Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophias
Provedor de dados: 5
Autores: Toullec, Jean-yves; Corre, Erwan; Bernay, Benoit; Thorne, Michael A. S.; Cascella, Kevin; Ollivaux, Celine; Henry, Joel; Clark, Melody S.
Background:  The Ice krill, Euphausia crystallorophias is one of the species at the base of the Southern Ocean food chain. Given their significant contribution to the biomass of the Southern Ocean, it is vitally important to gain a better understanding of their physiology and, in particular, anticipate their responses to climate change effects in the warming seas around Antarctica.  Methodology/Principal Findings:  Illumina sequencing was used to produce a transcriptome of the ice krill. Analysis of the assembled contigs via two different methods, produced 36 new pre-pro-peptides, coding for 61 neuropeptides or peptide hormones belonging to the following families: Allatostatins (A, B et C), Bursicon (a and b), Crustacean Hyperglycemic Hormones (CHH and...
Tipo: Text
Ano: 2013
URL: https://archimer.ifremer.fr/doc/00293/40444/39080.pdf

Deep sequencing of the mantle transcriptome of the great scallop Pecten maximus
Provedor de dados: 5
Autores: Artigaud, Sebastien; Thorne, Michael A. S.; Richard, Joelle; Lavaud, Romain; Jean, Fred; Flye-sainte-marie, Jonathan; Peck, Lloyd S.; Pichereau, Vianney; Clark, Melody S.
RNA-Seq transcriptome data were generated from mantle tissue of the great scallop, Pecten maximus. The consensus data were produced from a time course series of animals subjected to a 56-day thermal challenge at 3 different temperatures. A total of 26,064 contigs were assembled de novo, providing a useful resource for both the aquaculture community and researchers with an interest in mollusc shell production.
Tipo: Text
Palavras-chave: Pecten maximus; RNAseq; Temperature.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00202/31362/82662.pdf

Adult acclimation to combined temperature and pH stressors significantly enhances reproductive outcomes compared to short-term exposures
Provedor de dados: 5
Autores: Suckling, Coleen C.; Clark, Melody S.; Richard, Joelle; Morley, Simon A.; Thorne, Michael A. S.; Harper, Elizabeth M.; Peck, Lloyd S.
This study examined the effects of long-term culture under altered conditions on the Antarctic sea urchin, Sterechinus neumayeri.Sterechinus neumayeri was cultured under the combined environmental stressors of lowered pH (-03 and -05 pH units) and increased temperature (+2 degrees C) for 2years. This time-scale covered two full reproductive cycles in this species and analyses included studies on both adult metabolism and larval development. Adults took at least 6-8months to acclimate to the altered conditions, but beyond this, there was no detectable effect of temperature or pH. Animals were spawned after 6 and 17months exposure to altered conditions, with markedly different outcomes. At 6months, the percentage hatching and larval survival rates were...
Tipo: Text
Palavras-chave: CO2; Echinoderm; Gonad maturation; Larval development; Oxygen consumption; Vitellogenesis.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00617/72874/71930.pdf

Deciphering the molecular adaptation of the king scallop (Pecten maximus) to heat stress using transcriptomics and proteomics
Provedor de dados: 5
Autores: Artigaud, Sebastien; Richard, Joelle; Thorne, Michael A. S.; Lavaud, Romain; Flye-sainte-marie, Jonathan; Jean, Fred; Peck, Lloyd S.; Clark, Melody S.; Pichereau, Vianney.
Background: The capacity of marine species to survive chronic heat stress underpins their ability to survive warming oceans as a result of climate change. In this study RNA-Seq and 2-DE proteomics were employed to decipher the molecular response of the sub-tidal bivalve Pecten maximus, to elevated temperatures. Results: Individuals were maintained at three different temperatures (15, 21 and 25 degrees C) for 56 days, representing control conditions, maximum environmental temperature and extreme warming, with individuals sampled at seven time points. The scallops thrived at 21 degrees C, but suffered a reduction in condition at 25 degrees C. RNA-Seq analyses produced 26,064 assembled contigs, of which 531 were differentially expressed, with putative...
Tipo: Text
Palavras-chave: Marine biology; Metabolism; DNA repair; Transcription regulation; Apoptosis; Energy reserves.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00301/41173/40360.pdf