Consulta: creatorFacets:"Tourbiez, Delphine"
Registros recuperados: 30
Data/hora: 07/06/2024 11:12:27

Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1
Provedor de dados: 5
Autores: Segarra, Amelie; Mauduit, Florian; Faury, Nicole; Trancart, Suzanne; Degremont, Lionel; Tourbiez, Delphine; Haffner, Philippe; Barbosa-solomieu, Valerie; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan.
Background:  Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches.  Results:  The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant...
Tipo: Text
Palavras-chave: Interactions; Ostreid herpesvirus 1; Gene expression; Susceptibility; Crassostrea gigas.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00199/31029/29431.pdf

Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat
Provedor de dados: 5
Autores: Segarra, Amelie; Baillon, Laury; Faury, Nicole; Tourbiez, Delphine; Renault, Tristan.
High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerning the viral entry and its distribution in organs and tissues. This study compares OsHV-1 DNA and RNA detection and localization in experimentally infected oysters using two virus doses: a low dose that did not induce any mortality and a high dose inducing high mortality. Real time PCR demonstrated significant differences in terms of viral DNA amounts between the two virus doses. RNA transcripts were detected in oysters receiving the highest dose of viral...
Tipo: Text
Palavras-chave: Crassostrea gigas; In situ hybridization; Ostreid herpesvirus 1; Viral DNA; Viral RNA.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00300/41099/40272.pdf

Autophagy in Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Moreau, Pierrick; Moreau, Kevin; Morga, Benjamin; Tourbiez, Delphine; Travers, Marie-agnes; Rubinsztein, David; Renault, Tristan.
Mass mortality outbreaks of Pacific oysters, Crassostrea gigas, are reported in different areas around the world affecting seriously the shellfish aquaculture sector. Two pathogens are associated with these mortality outbreaks, ostreid herpesvirus 1 (OsHV-1) and Vibrio aestuarianus. In this contexte, a better knowledge is needed in terms of oyster immunity. For this purpose, as an important degradation pathway autophagy was investigated in the Pacific oysters. An in silico research of genes involved in autophagy using the C. gigas genome allowed identification of homologs of ATG1 and ATG8/LC3(Microtubule-associated protein 1A/1B-light chain 3). Gene expression and protein detection were analysed using real-time PCR and western blot, respectively. ATG1 and...
Tipo: Text
Palavras-chave: Autophagy; Pacific oyster; Crassostrea gigas; OsHV-1; Vibrio aestuarianus.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00218/32876/31345.pdf

Bilan 2015 du dispositif national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Lupo, Coralie; Osta Amigo, Axel; Fleury, Elodie; Robert, Stephane; Garcia, Celine; Arzul, Isabelle; Baillon, Laury; Bechemin, Christian; Canier, Lydie; Chollet, Bruno; Dechamps, Lucie; Dubreuil, Christine; Faury, Nicole; Francois, Cyrille; Godfrin, Yoann; Lapegue, Sylvie; Morga, Benjamin; Travers, Marie-agnes; Tourbiez, Delphine; Masson, Jean-claude; Verin, Francoise; Cordier, Remy; Gangnery, Aline; Louis, Wilfried; Mary, Charlotte; Normand, Julien; Penot, Julia; Cheve, Julien; Dagault, Francoise; Le Jolivet, Aurore; Le Gal, Dominique; Lebrun, Luc; Bellec, Gwenael; Bouget, Jean-francois; Cochennec-laureau, Nathalie; Palvadeau, Hubert; Grizon, James; Chabirand, Jean-michel; Pepin, Jean-francois; Seugnet, Jean-luc; D'Amico, Florence; Maurer, Daniele; Le Gall, Patrik; Mortreux, Serge; Baldi, Yoann; Orsoni, Valerie; Bouchoucha, Marc; Le Roy, Valerian; Pouvreau, Stephane; Queau, Isabelle; Lamoureux, Alice.
Depuis 1992, la surveillance de la santé des mollusques marins du littoral français est assurée par le réseau de Pathologie des Mollusques (Repamo). Ses activités s’inscrivent dans le cadre de la Directive Européenne 2006/88/CE. Depuis son évaluation par la plateforme nationale d’épidémiosurveillance en santé animale en 2012, l’objectif de surveillance est la détection précoce des infections dues à des organismes pathogènes exotiques et émergents affectant les mollusques marins sauvages et d’élevage. L’année 2015 est la première année de transition pour laquelle un début d’évolution des modalités de surveillance de la santé des mollusques marins animées par l’Ifremer a été amorcé. Un dispositif hybride de surveillance a été mis en place, s’appuyant sur...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Mortalité; Maladie.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00324/43486/42945.pdf

Autophagy plays an important role in protecting Pacific oysters from OsHV-1 and Vibrio aestuarianus infections
Provedor de dados: 5
Autores: Moreau, Pierrick; Moreau, Kevin; Segarra, Amelie; Tourbiez, Delphine; Travers, Marie-agnes; Rubinsztein, David C.; Renault, Tristan.
Recent mass mortality outbreaks around the world in Pacific oysters, Crassostrea gigas, have seriously affected the aquaculture economy. Although the causes for these mortality outbreaks appear complex, infectious agents are involved. Two pathogens are associated with mass mortality outbreaks, the virus ostreid herpesvirus 1 (OsHV-1) and the bacterium Vibrio aestuarianus. Here we describe the interactions between these 2 pathogens and autophagy, a conserved intracellular pathway playing a key role in innate immunity. We show for the first time that autophagy pathway is present and functional in Pacific oysters and plays an important role to protect animals from infections. This study contributes to better understand the innate immune system of Pacific...
Tipo: Text
Palavras-chave: Autophagy; Crassostrea gigas; OsHV-1; Pacific oyster; Vibrio aestuarianus; ATG; Autophagy-related; Atg8-PE; Atg8-phosphatidylethenolamine; DNA; Deoxyribonucleic acid; Hpi; Hours postinfection; LC3-II; Cleaved; Lipidated and autophagosome-associated form of LC3; MAP1LC3A; B (LC3A; B); Microtubule-associated proteins 1 light chain 3 alpha.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00269/37989/36072.pdf

Ecologically realistic model of infection for exploring the host damage caused by Vibrio aestuarianus
Provedor de dados: 5
Autores: Parizadeh, Leila; Tourbiez, Delphine; Garcia, Celine; Haffner, Philippe; Degremont, Lionel; Le Roux, Frederique; Travers, Marie-agnes.
Although vibrios are frequently associated with marine organisms mortality outbreaks, knowledge on their ecology and pathogenicity is sparse, thus limiting disease management and prophylactic strategies. Here, we investigated V. aestuarianus infection onset and progression in the wild, taking advantage of a “claire” pond: a semi‐closed system with limited seawater renewal, theoretically more adapted to disease transmission. We showed a positive association of the bacteria with oysters, which can constitute a reservoir for the bacteria in the winter. Moreover, passage through oysters was found to be necessary for experimental disease reproduction as vibrios shedding from diseased oysters has higher infectivity than from in vitro grown. We next developed an...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00446/55761/57423.pdf

A single regulatory gene is sufficient to alter Vibrio aestuarianus pathogenicity in oysters
Provedor de dados: 5
Autores: Goudenege, David; Travers, Marie-agnes; Lemire, Astrid; Petton, Bruno; Haffner, Philippe; Labreuche, Yannick; Tourbiez, Delphine; Mangenot, Sophie; Calteau, Alexandra; Mazel, Didier; Nicolas, Jean-louis; Jacq, Annick; Le Roux, Frederique.
Oyster diseases caused by pathogenic vibrios pose a major challenge to the sustainability of oyster farming. In France since 2012 a disease affecting specifically adult oysters has been associated with the presence of Vibrio aestuarianus. Here, by combining genome comparison, phylogenetic analyses and high throughput infections of strains isolated before or during the recent outbreaks, we show that virulent strains cluster into two V. aestuarianus lineages independently of the sampling dates. The bacterial lethal dose was not different between strains isolated before or after 2012. Hence the emergence of a new highly virulent clonal strain is unlikely. Each lineage comprises nearly identical strains, the majority of them being virulent, suggesting that...
Tipo: Text
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00226/33685/32530.pdf

Ostreid herpesvirus type 1 replication and host response in adult Pacific oysters, Crassostrea gigas
Provedor de dados: 5
Autores: Segarra, Amelie; Baillon, Laury; Tourbiez, Delphine; Benabdelmouna, Abdellah; Faury, Nicole; Bourgougnon, Nathalie; Renault, Tristan.
Since 2008, massive mortality outbreaks associated with OsHV-1 detection have been reported in Crassostrea gigas spat and juveniles in several countries. Nevertheless, adult oysters do not demonstrate mortality in the field related to OsHV-1 detection and were thus assumed to be more resistant to viral infection. Determining how virus and adult oyster interact is a major goal in understanding why mortality events are not reported among adult Pacific oysters. Dual transcriptomics of virus-host interactions were explored by real-time PCR in adult oysters after a virus injection. Thirty-nine viral genes and five host genes including MyD88, IFI44, IkB2, IAP and Gly were measured at 0.5, 10, 26, 72 and 144 hours post infection (hpi). No viral RNA among the 39...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00213/32414/30887.pdf

Experimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: Kinetics of virus DNA detection by q-PCR in seawater and in oyster samples
Provedor de dados: 5
Autores: Schikorski, David; Faury, Nicole; Pepin, Jean-francois; Saulnier, Denis; Tourbiez, Delphine; Renault, Tristan.
Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crassostrea gigas.  In the present work, we have developed an experimental protocol of horizontal transmission based on the cohabitation between healthy and experimentally infected oysters. Through a cohabitation trial, the kinetics of OsHV-1 detection in different oyster organs and seawater samples were investigated and characterized for the first time using real time quantitative PCR.
Tipo: Text
Palavras-chave: Ostreid herpesvirus 1; Cupped Pacific oyster; Crassostrea gigas; Experimental infection; Kinetic viral DNA quantification.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00025/13607/10688.pdf

Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus -related and V. aestuarianus strains from bacterial cultures
Provedor de dados: 5
Autores: Saulnier, Denis; De Decker, Sophie; Tourbiez, Delphine; Travers, Marie-agnes.
To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies.
Tipo: Text
Palavras-chave: Multiplex real-time PCR; Gene sequencing; Molluscs; Splendidus Glade; Vibrio aestuarianus.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00392/50308/51077.pdf

Bilan 2009 du réseau REPAMO - Réseau national de surveillace de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Miossec, Laurence; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Cobret, Laetitia; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Breerette, Stephane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Banamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00086/19711/17352.pdf

Can selection for resistance to OsHV-1 infection modify susceptibility to Vibrio aestuarianus infection in Crassostrea gigas? First insights from experimental challenges using primary and successive exposures
Provedor de dados: 5
Autores: Azema, Patrick; Travers, Marie-agnes; De Lorgeril, Julien; Tourbiez, Delphine; Degremont, Lionel.
Since 2008, the emergent virus OsHV 1μvar has provoked massive mortality events in Crassostrea gigas spat and juveniles in France. Since 2012, mortality driven by the pathogenic bacteria Vibrio aestuarianus has stricken market sized adults. A hypothesis to explain the sudden increase in mortality observed in France since 2012 is that selective pressure due to recurrent viral infections could have led to a higher susceptibility of adults to Vibrio infection. In our study, two OsHV-1-resistant lines (AS and BS) and their respective controls (AC and BC) were experimentally challenged in the laboratory to determine their level of susceptibility to V. aestuarianus infection. At the juvenile stage, the selected lines exhibited lower mortality (14 and 33%) than...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00299/41054/40215.pdf

Whole-genome amplification: a useful approach to characterize new genes in unculturable protozoan parasites such as Bonamia exitiosa
Provedor de dados: 5
Autores: Prado-alvarez, Maria; Couraleau, Yann; Chollet, Bruno; Tourbiez, Delphine; Arzul, Isabelle.
Bonamia exitiosa is an intracellular parasite (Haplosporidia) that has been associated with mass mortalities in oyster populations in the Southern hemisphere. This parasite was recently detected in the Northern hemisphere including Europe. Some representatives of the Bonamia genus have not been well categorized yet due to the lack of genomic information. In the present work, we have applied Whole-Genome Amplification (WGA) technique in order to characterize the actin gene in the unculturable protozoan B. exitiosa. This is the first protein coding gene described in this species. Molecular analysis revealed that B. exitiosa actin is more similar to Bonamia ostreae actin gene-1. Actin phylogeny placed the Bonamia sp. infected oysters in the same clade where...
Tipo: Text
Palavras-chave: Bonamia exitiosa; Bonamia ostreae; Actin; Haplosporidia; Whole-Genome Amplification.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00277/38871/37410.pdf

Bilan 2013 du réseau Repamo - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Tourbiez, Delphine; Chollet, Bruno; Faury, Nicole; Haffner, Philippe; Dubreuil, Christine; Serpin, Delphine; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Cheve, Julien; Penot, Julia; Le Gal, Dominique; Lebrun, Luc; Le Gac-abernot, Chantal; Langlade, Aime; Bedier, Edouard; Palvadeau, Hubert; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrick; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude.
Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Repamo a pour objectif de détecter précocement les infections dues à des organismes pathogènes exotiques et émergents affectant les mollusques marins sauvages et d’élevage.  L’étude des hausses de mortalités a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la collecte d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités d’huîtres creuses (Crassostrea gigas) (44 interventions) ont été recensées dans la majorité des bassins de production de mai à...
Tipo: Text
Palavras-chave: Réseau Surveillance; Pathologie; Mollusques; Coquillages; Santé.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00197/30798/29167.pdf

Expression de gènes viraux de l'herpès virus OsHV-1 sous sa forme µVar chez l'huître creuse, Crassostrea gigas
Provedor de dados: 5
Autores: Segarra, Amelie; Faury, Nicole; Haffner, Philippe; Pepin, Jean-francois; Tourbiez, Delphine; Trancart, Suzanne; Travers, Marie-agnes; Renault, Tristan.
Le premier cas d'infection à virus de type herpès a été rapporté en 1972, aux Etats Unis, chez l'huître américaine, Crassostrea virginica (Farley et al, 1972). A partir de 1991, des mortalités massives associées à la détection de virus de type herpes ont été observées chez plusieurs espèces de bivalves marins, à différents stades de développement (essentiellement chez les larves et le naissain) et dans différentes régions du monde. Sur la base d’observation en microscopie électronique à transmission, ces virus présentaient des caractéristiques morphologiques identiques à celles des Herpesvirus (Roizman, 1982).  Les connaissances acquises concernant la taille, la structure et le séquençage du génome d’un virus infectant les larves d’huîtres creuses en...
Tipo: Text
Palavras-chave: Herpès virus OsHV-1; Expression gènes; Huître creuse; Crassostrea gigas.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00195/30627/29076.pdf

Bilan 2012 du réseau REPAMO - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Pepin, Jean-francois; Hatt, Philippe-jacques; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Huchet, Eve; Dubreuil, Christine; Chollet, Bruno; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Annezo, Jean-pierre; Terre-terrillon, Aouregan; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Hittier, Benoist; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrik; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude.
Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à réglementés et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font partie des missions institutionnelles de l’IFREMER.   L’étude des hausses de mortalités (protocole II) a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la réalisation d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités...
Tipo: Text
Palavras-chave: Réseau; Pathologie; Mollusques; Coquillages; Santé; Surveillance.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00143/25470/23625.pdf

A European epidemiological survey of Vibrio splendidus clade shows unexplored diversity and massive exchange of virulence factors
Provedor de dados: 5
Autores: Nasfi, H.; Travers, Marie-agnes; De Lorgeril, Julien; Habib, C.; Sannie, T.; Sorieul, L.; Gerard, J.; Avarre, J. C.; Haffner, Philippe; Tourbiez, Delphine; Renault, Tristan; Furones, D.; Roque, A.; Pruzzo, C.; Cheslett, D.; Gdoura, R.; Vallaeys, T.
The Vibrio splendidus clade has previously been associated with epidemic outbreaks of various aquatic animals, as in the case of the cupped oyster, Crassostrea gigas. To investigate whether involved strains could present a clonal origin and to identify possible alternative background carriage animals or zooplankton, a large epidemiological survey was conducted on isolates of the splendidus clade. For this purpose, Vibrio strains were isolated from various samples including oysters, mussels, sediments, zooplankton, and sea water on the basis of a North/South gradient of the European sea water zone (Ireland, The Netherlands, France, Italy, and Spain). A total of 435 isolates were successfully associated to the V. splendidus clade using real time polymerase...
Tipo: Text
Palavras-chave: VNTR; Vibrio splendidus; Epidemiology; Vsm; OmpU; Crassotrea giga; Mortality.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00255/36666/35350.pdf

Is horizontal transmission of the Ostreid herpesvirus OsHV-1 in Crassostrea gigas affected by unselected or selected survival status in adults to juveniles?
Provedor de dados: 5
Autores: Degremont, Lionel; Guyader, Tanguy; Tourbiez, Delphine; Pepin, Jean-francois.
Massive mortality outbreaks, mostly affecting spat Crassostrea gigas, have been reported in France since 2008. Disease investigations revealed that most of the mortality events are related to the detection of the Ostreid herpesvirus 1 (OsHV-1 μVar). Meanwhile, selection to improve the survival in juvenile C. gigas has been successfully implemented in this context and selected oysters were found disease-resistant to OsHV-1 infection. This paper reports the first investigation of the horizontal transmission of the disease throughout cohabitation trials within batch and between batches using unselected and selected oysters for their higher survival. Two batches, unselected (AC) and selected (AR), were produced in February 2009 and deployed in the field in...
Tipo: Text
Palavras-chave: Crassostrea gigas; Disease resistance; Ostreid herpesvirus OsHV-1; Horizontal transmission; Pacific oyster.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00142/25310/23383.pdf

Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov.
Provedor de dados: 5
Autores: Garcia, Celine; Mesnil, Aurelie; Tourbiez, Delphine; Moussa Pouly, Mirna; Dubreuil, Christine; Gonçalves De Sa, Amélie; Chollet, Bruno; Godfrin, Yoann; Dégremont, Lionel; Serpin, Delphine; Travers, Marie-agnes.
Cockle mortality events have been reported in northern France since 2012. In the present study, we describe and investigate the implication of a potential bacterial causative agent in cockle mortality. Bacteria isolated from five different cockle mortality events were characterized and studied. Using phenotypic analysis combined with DNA–DNA hybridization (DDH) and whole genome sequencing, the isolates were shown to belong to Vibrio aestuarianus , a species regularly detected in France during oyster mortality events. Comparison of the strains from cockles with strains from French oysters and the type strain showed that the strains from cockles were genetically different to those from oysters and also different to the V. aestuarianus type strain. Moreover,...
Tipo: Text
Palavras-chave: Bacterial pathogen; Bivalvia; Cockle; Mortality; Shellfish; Vibrio aestuarianus.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00677/78893/81195.pdf

Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Travers, Marie-agnes; Tourbiez, Delphine; Parizadeh, Leila; Haffner, Philippe; Kozic-djellouli, Angelique; Aboubaker, Mohamed; Koken, Marcel; Degremont, Lionel; Lupo, Coralie.
This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00386/49768/50308.pdf

Mortalités massives de l'huître creuse - Rapport final du programme de recherche sur l'étude de la cinétique de détection d'agents infectieux associés à des épisodes de mortalités de naissains d'huîtres creuses Crassostrea gigas sur un site ostréicole de Marennes-Oléron (CIDAGINF 2009-2010)
Provedor de dados: 5
Autores: Saulnier, Denis; Pepin, Jean-francois; Guesdon, Stephane; Degremont, Lionel; Faury, Nicole; Haffner, Philippe; Renault, Tristan; Travers, Marie-agnes; Tourbiez, Delphine; Geairon, Philippe; Le Moine, Olivier; Seugnet, Jean-luc; Soletchnik, Patrick.
This study aims to improve understanding of causes of mortalities in oyster Crassostrea gigas in pertuis charentais related to environmental parameters and the kinetics of targeted infectious agents, such as bacteria Vibrio splendidus (group ) and V. aestuarianus and Ostreid herpesvirus OsHV-1. Two sampling campaigns were carried out on the site of Agnas in 2009 and 2010, before, during or following mortality events of spat from March to September. The approach is to detect and quantify in short time step infectious agents from tissues of farmed oysters placed on rack and in various samples taken nearby: native oysters and other species (wild mussels and barnacles), seawater and sediment. With physical and chemical automatic probes placed on the site,...
Tipo: Text
Palavras-chave: Huître creuse; Crassostrea gigas; Mortalité; Virus herpès; Vibrions; Environnement; Pertuis Charentais; Pacific oyster; Crassostrea gigas; Mortality; Herpes virus; Vibrios; Environment; Pertuis Charentais.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00058/16920/14400.pdf

Does global warming favour the occurrence of recent blue mussel mortality events in France?
Provedor de dados: 5
Autores: Pepin, Jean-francois; Soletchnik, Patrick; Le Moine, Olivier; Polsenaere, Pierre; Genauzeau, Sylvie; Robert, Stephane; Grizon, James; Seugnet, Jean-luc; Schmitt, Anne; Chabirand, Jean-michel; Tourbiez, Delphine; Travers, Marie-agnes; Guesdon, Stephane.
We report in this work data of two independent studies carried out in the Pertuis Charentais for which a link might exist? A 30-years local time series study of hydro-climatic variations in water temperature and salinity, which show a significant warming of the water masses in the Marennes Oleron bay (+ 1.5 °C) associated with a significant drop ( &gt; -15%) of freshwater intakes by nearby rivers. In this changing local climate context several major epizootics have appeared, severely affecting farmers and the production of Pacific oysters and mussels, from the first French shellfish-producing department (catching and growing, Agreste, 2012). Following massive mortality event of blue mussels in 2014, a specific study (MORBLEU) is conducted, trying to...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00415/52697/53567.pdf

Development of TaqMan real-time PCR assays for monitoring Vibrio harveyi infection and a plasmid harbored by virulent strains in European abalone Haliotis tuberculata aquaculture
Provedor de dados: 5
Autores: Schikorski, David; Renault, Tristan; Paillard, Christine; Bidault-toffin, A.; Tourbiez, Delphine; Saulnier, Denis.
The Gram-negative bacterium Vibrio harveyi is known to be highly pathogenic for the European abalone Haliotis tuberculata, which is a gastronomically important marine gastropod with a high commercial value. Since 1998, some particular bacterial strains are described as implicated in recurrent mortality outbreaks in French farm and field stocks of abalone. Recently, a 9.6kb plasmid named pVCR1, was shown to be harbored by one highly V. harveyi virulent ORM4 strain suggesting its involvement in virulence phenotype. Thus, we have developed in the present study two TaqMan real-time PCR assays allowing to (i) rapidly and specifically detect, by a duplex procedure and in less than 2 h, both V. harveyi and the presence of plasmid pVCR1 from unidentified bacterial...
Tipo: Text
Palavras-chave: Vibrio harveyi; Haliotis tuberculata; Real-time PCR; Plasmid; Marine pathogen; Molecular diagnostic.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00124/23540/21383.pdf

Bilan 2008 du réseau REPAMO - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Tourbiez, Delphine; Omnes, Emmanuelle; Cobret, Laetitia; Faury, Nicole; Haffner, Philippe; Saulnier, Denis; Pepin, Jean-francois; Renault, Tristan; Antajan, Elvire; Rauflet, Fabienne; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00086/19709/17351.pdf

Bilan 2010 du réseau Repamo - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Guichard, Benjamin; Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Brerette, Stéphane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam; Cantin, Christian.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l’IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l’huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Surveillance.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00059/17050/14599.pdf

Modeling the Transmission of Vibrio aestuarianus in Pacific Oysters Using Experimental Infection Data
Provedor de dados: 5
Autores: Lupo, Coralie; Travers, Marie-agnes; Tourbiez, Delphine; Barthélémy, Clement; Beaunée, Gaël; Ezanno, Pauline.
Vibrio aestuarianus is a bacterium related to mortality outbreaks in Pacific oysters, Crassostrea gigas, in France, Ireland, and Scotland since 2011. Knowledge about its transmission dynamics is still lacking, impairing guidance to prevent and control the related disease spread. Mathematical modeling is a relevant approach to better understand the determinants of a disease and predict its dynamics in imperfectly observed pathosystems. We developed here the first marine epidemiological model to estimate the key parameters of V. aestuarianus infection at a local scale in a small and closed oyster population under controlled laboratory conditions. Using a compartmental model accounting for free-living bacteria in seawater, we predicted the infection dynamics...
Tipo: Text
Palavras-chave: Marine epidemiology; Parameter estimation; Compartmental model; ABC method; Global sensitivity analysis; Basic reproduction number R-0; Crassostrea gigas; Oyster mortality.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00495/60652/64148.pdf

Spatial epidemiological modelling of infection by Vibrio aestuarianus shows that connectivity and temperature control oyster mortality
Provedor de dados: 5
Autores: Lupo, Coralie; Dutta, Bhagat Lal; Petton, Sebastien; Ezanno, P; Tourbiez, Delphine; Travers, Marie-agnes; Pernet, Fabrice; Bacher, Cedric.
Vibrio aestuarianus infection in oyster populations causes massive mortality, resulting in losses for oyster farmers. Such dynamics result from host-pathogen interactions and contagion through water-borne transmission. To assess the spatiotemporal spread of V. aestuarianus infection and associated oyster mortality at a bay scale, we built a mathematical model informed by experimental infection data at 2 temperatures and spatially dependent marine connectivity of oyster farms. We applied the model to a real system and tested the importance of each factor using a number of modelling scenarios. Results suggest that introducing V. aestuarianus in a fully susceptible adult oyster population in the bay would lead to the mortality of all farmed oysters over 6 to...
Tipo: Text
Palavras-chave: Basic reproduction number; Crassostrea gigas; Hydrodynamics; Oyster disease; Sensitivity analysis; Vibrio aestuarianus.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00662/77459/79114.pdf

First description of French V. tubiashii strains pathogenic to mollusk: I. Characterization of isolates and detection during mortality events
Provedor de dados: 5
Autores: Travers, Marie-agnes; Mersni Achour, Rachida; Haffner, Philippe; Tourbiez, Delphine; Cassone, Anne-laure; Morga, Benjamin; Doghri, Ibtissem; Garcia, Celine; Renault, Tristan; Fruitier-arnaudin, Ingrid; Saulnier, Denis.
Nine dominant bacterial isolates were obtained from different batches of Crassostrea gigas spat experiencing high mortality rates in a French experimental hatchery/nursery in 2007. Using phenotypic analysis combined with multilocus sequence analysis, the isolates were shown to be genetically close to the Vibrio tubiashii type strain. Based on (1) analyses of the recA gene sequences; (2) the results of DNA–DNA hybridization assays between 07/118 T2 (LMG 27884 = CECT 8426), which is a representative strain, and the V. tubiashii type strain (69%); and (3) phenotypic traits, the bacteria were classified in a group close to American V. tubiashii strain. Its virulence (70% of mortalities) and the toxicity of the extracellular products of 07/118 T2 was...
Tipo: Text
Palavras-chave: Crassostrea gigas Pathogenicity Extracellular products Polyphasic approach; Real time PCR.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00189/30028/28743.pdf

Niveau de ploïdie des naissains d'huître creuse captés dans les bassins de Marennes Oléron et d'Arcachon. Réseau Biovigilance, campagne 2009
Provedor de dados: 5
Autores: Benabdelmouna, Abdellah; Tourbiez, Delphine; D'Amico, Florence; Cantin, Christian; Grizon, James; Seugnet, Jean-luc.
En 2009, La caractérisation du niveau de ploïdie des naissains captés en 2008 dans les deux principaux bassins de captage que sont Marennes Oléron et Arcachon a été réalisée dans le cadre du réseau biovigilance. Les échantillons des naissains sauvages issus de ce captage naturel ont été prélevés sur 3 sites dans chaque bassin et pour un total analysé de 1121 animaux. En tenant compte de notre échantillonnage, nos résultats de suivi de ploïdie concluent à l’absence d’animaux polyploïdes, triploïdes ou tétraploïdes, captés dans les deux bassins prospectés. Cependant, les deux bassins de captage rospectés sont caractérisés par la présence accrue de naissains affectés de diverses anomalies génomiques (cassures d’ADN et diminution de la taille du génome,...
Tipo: Text
Palavras-chave: Huître creuse; Crassostrea gigas; Génétique; Ploïdie; Naissain; Bassin Marennes Oléron; Charente-Maritime; Bassin d'Arcachon; Gironde.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00032/14282/11559.pdf

Première évaluation de la diversité des communautés microbiennes planctoniques au cours d'épisodes de mortalité des moules bleues en France : utilisation de l'approche de métabarcoding pour l'analyse de l'ADN environnemental
Provedor de dados: 5
Autores: Pepin, Jean-francois; Bouget, Jean-francois; Chabirand, Jean-michel; Chasselin, Leo; Costes, Louis; Grizon, James; Lejolivet, Aurore; Le Noc, Sandrine; Palvadeau, Hubert; Schmitt, Anne; Seugnet, Jean-luc; Soletchnik, Patrick; Travers, Marie-agnes; Tourbiez, Delphine; Guesdon, Stephane.
Since 2014 in France, in Charente maritime and Vendée, farmed and stocks of blue mussels experienced strong mortality events. In this context, the « MORBLEU » research project (DPMA-Ifremer agreement) was initiated to explore potentially aggravating factors, associated or correlated with mortality of mussels. Here, the work is to study biotic factors in mussels environment, looking for describe diversity and evolution of microbial communities (water column) before, during and after mortality events using NGS technology (metabarcoding) for environmental DNA analysis. We present, sampling strategy used from 2015 to 2018, Material &amp; Methods and some preliminary results.  
Tipo: Text
Palavras-chave: MORBLEU; Moules; Mortalité; ADN environnemental; EDNA; Metabarcoding; Communautés microbiennes.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00505/61664/65579.pdf