Consulta: creatorFacets:"Tovar, Ana Maria Freire"
Registros recuperados: 4
Data/hora: 02/06/2024 16:07:40

Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds
Provedor de dados: 3
Autores: Rodrigues, José Ariévilo Gurgel; Barcellos, Priscila Gomes; Glauser, Bianca Fernandes; Quinderé, Ana Luíza Gomes; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de Souza.
The biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and C. racemosa) were analyzed and compared for their abundance, physical-chemical characteristics and in vitro anticoagulant assays of activated partial thromboplastin time-APTT, prothrombin time-PT and TG. Papain extraction yielded (p &lt; 0.001) from 0.66±0.03% (C. racemosa) to 41.60±1.10% (Halymenia sp.) of crude SPs  varying sulfate (8.41-42.60%) and total sugars (47.80-70.53%). Crude SPs showed difference in mobility and resolution pattern by agarose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine algae; Polyglycans; Coagulation tests; Thrombin.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463

Molecular characteristics of polysulfated fractions from the green seaweed Caulerpa cupressoides and actions on thrombin generation in vitro
Provedor de dados: 3
Autores: Rodrigues, José Ariévilo Gurgel; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de Souza.
Caulerpa cupressoides produces sulfated polysaccharides (Cc-SPs) with serpin-dependent anticoagulant effect, but their actions on thrombin generation (TG) are unknown. This study aimed to partially characterize Cc-SPs and examine their potential as modulators of TG. Infrared analysis characterized extract containing three ulvan fractions (Cc-SP1, -SP2 and -SP3) separated by DEAE-cellulose chromatography, with differences in the relative proportions of sulfate (10.99-18.38%) and total sugars (46.59-51.12%), without presenting proteins. Charge density patterns and nonSPs varying from 8 to &gt; 100 kDa on agarose and polyacrylamide gel electrophoresis by sequential staining with toluidine blue and stains-all were also confirmed by gel permeation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caulerpaceae; Marine alga; Sulfated glycans; Thrombosis.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/35655

Mild-acid hydrolysis of a native polysulfated fraction from Acanthophora muscoides generates sulfated oligosaccharides displaying in vitro thrombin generation inhibition
Provedor de dados: 3
Autores: Rodrigues, José Ariévilo Gurgel; Queiroz, Ismael Nilo Lino de; Quinderé, Ana Luíza Gomes; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de Souza.
Acanthophora muscoides (Rhodophyta) has three polysulfated fractions    (Am-1, Am-2 and Am-3). Am-2 displayed anti-inflammation and serpin-independent anticoagulation; however, no effect of oligomers on thrombin-generation (TG) assay has been demonstrated. This study employed mild-acid hydrolysis to obtain low-molecular-size derivatives from Am-2 and compared in vitro inhibitory effects between intact Am-2 and its hydrolysates on a TG assay. The enzymatic extract was fractionated by DEAE-cellulose that revealed Am-2 eluted with 0.75-M NaCl containing sulfate (23%), hexoses (51%) and devoid of proteins, and indicating, by one-dimension nuclear magnetic resonance, structure of galactan similar to that of extract. The depolymerization with HCl (0.02 or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ciências Biológicas e Ambientais Rhodophyceae; Poli-aniônicos; Depolimerização; Trombina. Química de macromoléculas Rhodophyceae; Polyanionics; Depolymerization; Thrombin.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28257

In vitro inactivation of thrombin generation by polysulfated fractions isolated from the tropical coenocytic green seaweed Caulerpa racemosa (Caulerpaceae, Bryopsidales)
Provedor de dados: 3
Autores: Rodrigues, José Ariévilo Gurgel; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de Souza.
 Pharmacological efficacy of Caulerpa racemosa (Chlorophyta) sulfated polysaccharidic (SPs) fractions (F I→III) on models of coagulation and inflammation has been demonstrated, but not their effects on thrombin generation (TG). This study examined fractions for composition and physical-chemical characteristics and in vitro inactivation of TG by F I and F II in 60-fold diluted human plasma using continuous method. Papain-extraction yield of 0.7% revealed F I→III by DEAE-cellulose chromatography, with differences among the relative proportions of sulfate (17.37-24.00%), total sugars (30.03-48.34%) and absence of proteins. Charge density patterns and molecular sizes &gt; 100 kDa of the fractions were verified by both agarose/polyacrylamide analyses,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorophyta; Complex glycans; Sulfation pattern; Clot.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/32095