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Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent
Provedor de dados: 58
Autores: Hoshino-Shimizu,Sumie; Vaz-de-Lima,Lourdes Rehder de Andrade; Oliveira,Maria Isabel de; Pereira,Carlos Augusto; Moura,Anísio de; Mendonça,Ronaldo Zucatelli.
Recent measles outbreaks in different countries led to an increase of laboratory measles diagnosis. Thus, we developed the IgM-Measles ELISA IAL, using measles virus antigens obtained from cell cultured in microcarriers in order to supply reagent kits to Brazilian public health laboratories. A batch of antigenic reagent was produced and evaluated in the enzyme immunoassay in comparison with clinical diagnosis and with as reference assay (IgM Capture ELISA CDC) data. This study was performed in a positive panel with 70 serum samples from patients with measles, and a negative panel with 132 samples from patients with unrelated diseases and without recent measles or vaccination history. In relation to other diagnostic methods, the IgM ELISA IAL presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Measles; IgM-measles reagent; Reagent quality control; Serodiagnosis; Seroepidemiology.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100016