Consulta: creatorFacets:"Verrez-bagnis, Veronique"
Registros recuperados: 32
Data/hora: 06/06/2024 15:53:03

Characterization of biofilm extracts from two marine bacteria
Provedor de dados: 5
Autores: Passerini, Delphine; Fecamp, Florian; Marchand, Laetitia; Kolypczuk, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Verrez-bagnis, Veronique; Hervio Heath, Dominique; Colliec Jouault, Sylvia; Delbarre Ladrat, Christine.
In the marine environment, biofilm formation is an important lifestyle for microorganisms. A biofilm is comprised of cells embedded in an extracellular matrix that holds them close together and keeps the biofilm attached to the colonized surface. This predominant lifestyle and its main regulation pathway, namely quorum-sensing (QS), have been shown to induce specific bioactive metabolites. In this study, we investigated the biofilm formation by two marine bacteria belonging to the Vibrio species to discover potentially innovative bioactive compounds. We proposed a protocol to isolate biofilm extracts, to analyze their biochemical composition, and to compare them to planktonic cell extracts. Cells were grown attached to a plastic surface; extracts were...
Tipo: Text
Palavras-chave: Vibrio; Biofilm; Polysaccharide; Quorum-sensing; Activity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00592/70381/68461.pdf

Surimi de chinchard ( Trachurus trachurus L. ) - Influence de conditions technologiques sur des caractéristiques de texture du produit après traitement thermique
Provedor de dados: 5
Autores: Verrez-bagnis, Veronique.
Surimi, washed fish mince after grinding with salt, has a firm and elastic gel texture or “ashi” after thermal processing. The major objectives of this study on horse mackerel (Trachurus trachurus) surimi are to optimize the technological conditions in the aim to obtain the best ashi, to determine the influence of frozen storage and of egg white and potata starch addition, and to establish the preventive effect of incorporation into surimi of different combinations of cryoprotectants and NaC1. Gel texture is, by order importance, dependent on cooking temperature, length of setting and cooking and length of grinding of surimi with texture additives after thawing. Changes of gelling properties after 6 months at - 30°C of frozen st orage are limited. The...
Tipo: Text
Palavras-chave: Fish; Horse mackerel (Trachurus trachurus L.); Surimi; Kamaboko; Myofibrillar proteins; Gelation; Thermal coagulation; Texture; Optimisation method; Technological treatments; Cryoprotectors; Texture ingredients; Microscopy; Water retention capacity.
Ano: 1987
URL: http://archimer.ifremer.fr/doc/00134/24507/22535.pdf

Calpain and cathepsin activities in post mortem fish and meat muscles
Provedor de dados: 5
Autores: Cheret, Romuald; Delbarre Ladrat, Christine; De Lamballerie Anton, Marie; Verrez-bagnis, Veronique.
Post mortem tenderization is one of the most unfavourable quality changes in fish muscle and this contrasts with muscle of mammalian meats. The tenderization can be partly attributed to the acid lysosomal cathepsins and cytosolic neutral calcium-activated calpains. In this study, these proteases from fish and bovine muscles were quantified and compared. The cathepsin B and L activities were in more important amounts in sea bass white muscle than in bovine muscle. On the other hand, cathepsin D activity was 1.4 times higher in meat that in fish muscle, while cathepsin H was negligible in both muscles. Calpain activities were similar in both types of muscle. Moreover, calpastatin (calpain endogenous inhibitor) level is 3.9 times higher in sea bass white...
Tipo: Text
Palavras-chave: Meat; Fish; Protease; Cathepsin; Calpain.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3646.pdf

Mining of the biosynthetic mechanisms of Vibrio spp. polysaccharides and potential role in biofilm formation
Provedor de dados: 5
Autores: Verrez-bagnis, Veronique; Soree, Marion; Passerini, Delphine; Kolypczuk, Laetitia; Marchand, Laetitia; Bonnetot, Sandrine; Fecamp, Florian; Lozach, Solen; Hervio Heath, Dominique; Delbarre Ladrat, Christine.
Vibrio spp. are ubiquitous marine bacteria that are ecologically and metabolically diverse members of planktonic and animal associated microbial communities. They encompass the ancient and well-studied human pathogen, Vibrio cholerae, and two other human pathogens, V. vulnificus and V. parahaemolyticus, as well as some less thoroughly characterized animal pathogens. Virulence is based on a wide diversity of mechanisms involved in motility and host colonization, in ability to persist and develop, and in damage generation. Polysaccharides may play major roles in virulence and are major components of extracellular polymeric matrix synthesized upon biofilm growth. They may also exhibit biological features, especially those similar to animal-derived...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00514/62605/66965.pdf

Effects of high pressure on texture and microstructure of sea bass (Dicentrarchus labrax L.) fillets
Provedor de dados: 5
Autores: Cheret, Romuald; Chapleau, Nicolas; Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; De Lamballerie, Marie.
High pressure is an innovative non-thermal food-preservation technology. We studied the effect of high-pressure treatment up-to 500 MPa for 5 min on physical characteristics of sea bass fillets after 0, 7, and 14 d of refrigerated storage.. Color results exhibited an increase of lightness and a slight change of hue, which might be imperceptible in cooked fish. High-pressure treatment induced a decrease of exudation and water-holding capacity Pressure treatment above 300 MPa provoked higher fish hardness after storage than in untreated sample, proving the ability of high pressure to improve textural, quality of chilled, stored fish fillet. These assessments were corroborated with microstructure observations. We showed that high-pressure treatment at 500 MPa...
Tipo: Text
Palavras-chave: Preservation; Microstructure; Texture profile analysis; High pressure; Fish.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-734.pdf

Proteolytic potential in white muscle of sea bass (Dicentrarchus labrax L.) during post mortem storage on ice: time-dependent changes in the activity of the components of the calpain system
Provedor de dados: 5
Autores: Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; Noel, Joelle; Fleurence, Joel.
The variations in the amounts of milli-calpain and its specific inhibitor in the white muscle of sea bass (Dicentrarchus labrax) during storage at 4 degreesC for up to 7 days were determined after separation by hydrophobic chromatography on a Phenyl Sepharose vel. There was a significant decline in post-slaughter m-calpain activity with an important inter-individual variability in the rate of decrease of the total activity. In contrast with the calpastatin of mammalian post mortem muscles, calpastatin remained constant within fish muscles after death. The initial levels of protease and inhibitor activities, and their behaviour through post mortem storage. are discussed and implications for the mechanism of tenderisation of fish muscle are suggested. (C)...
Tipo: Text
Palavras-chave: Proteolysis; Post mortem; Fish muscle; Calpain; Calpastatin; Neutral calcium dependent protease.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-1683.pdf

Proteins and proteolytic activity changes during refrigerated storage in sea bass (Dicentrarchus labrax L.) muscle after high-pressure treatment
Provedor de dados: 5
Autores: Cheret, Romuald; Hernandez Andres, Aránzazu; Delbarre Ladrat, Christine; De Lamballerie, Marie; Verrez-bagnis, Veronique.
Contrary to other preservation methods like thermal treatments, high pressure can destroy microorganisms without affecting the nutritional quality, color, or food texture. The firm texture of fish flesh is an important quality parameter. During the refrigerated storage, the tissue becomes softer and the muscle is deteriorated by different proteases. The aim of this study was to study the modification of the fish muscle proteins after high-pressure treatment during the refrigerated storage and to evaluate the effect of high-pressure treatment level on the post-mortem protein changes and enzyme activities. The calpain activity decreased with the high-pressure treatment and evolved differently during the refrigerated storage, depending on the level of...
Tipo: Text
Palavras-chave: Cathepsins; Calpains; Sarcoplasmic proteins; Myofibrillar proteins; Post mortem denaturation; High pressure; Fish.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1708.pdf

Relative contribution of calpain and cathepsins to protein degradation in muscle of sea bass (Dicentrarchus labrax L.)
Provedor de dados: 5
Autores: Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; Noel, Joelle; Fleurence, Joel.
The effects of a milli(m)-calpain isolated from the white muscle of sea bass (Dicentrarchus labrax L) and commercial cathepsins B, D and L, used in combination on the myofibrillar and sarcoplasmic proteins were examined. Protein digestion was first performed by the endogenous m-calpain, (luring 2 h before the addition of a mixture of cathepsins B, D and L and a further incubation up to 22 h. Calpain degraded a 27 kDa sarcoplasmic component as well as myosin heavy chain, a-actinin, desmin and a 32 kDa component from the myofibrillar fraction. A 97 kDa component and the assumed creatine kinase-aldolase doublet were degraded during the incubation of sarcoplasmic proteins with the cathepsin mixture while, among the myofibrillar proteins, myosin, actin,...
Tipo: Text
Palavras-chave: Postmortem aging; Fish muscle; Proteolysis; Cathepsins; Neutral calcium dependent protease.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-1682.pdf

Gene-associated markers provide tools for tackling illegal fishing and false eco-certification
Provedor de dados: 5
Autores: Nielsen, Einar E.; Cariani, Alessia; Mac Aoidh, Eoin; Maes, Gregory E.; Milano, Ilaria; Ogden, Rob; Taylor, Martin; Hemmer-hansen, Jakob; Babbucci, Massimiliano; Bargelloni, Luca; Bekkevold, Dorte; Diopere, Eveline; Grenfell, Leonie; Helyar, Sarah; Limborg, Morten T.; Martinsohn, Jann T.; Mcewing, Ross; Panitz, Frank; Patarnello, Tomaso; Tinti, Fausto; Van Houdt, Jeroen K. J.; Volckaert, Filip A. M.; Waples, Robin S.; Carvalho, Gary; Albin, Jan Ej; Vieites Baptista, Juan M; Barmintsev, Vladimir; Bautista, Jose M.; Bendixen, Christian; Berge, Jean-pascal; Blohm, Dietmar; Cardazzo, Barbara; Diez, Amalia; Espineira, Montserrat; Geffen, Audrey J.; Gonzalez, Elena; Gonzalez-lavin, Nerea; Guaniero, Ilaria; Jerome, Marc; Kochzius, Marc; Krey, Grigorius; Mouchel, Olivier; Negrisolo, Enrico; Piccinetti, Corrado; Puyet, Antonio; Rastorguev, Sergey; Smith, Jane P; Trentini, Massimo; Verrez-bagnis, Veronique; Volkov, Alexander; Zanzi, Antonella.
Illegal, Unreported and Unregulated fishing has had a major role in the overexploitation of global fish populations. In response, international regulations have been imposed and many fisheries have been 'eco-certified' by consumer organizations, but methods for independent control of catch certificates and eco-labels are urgently needed. Here we show that, by using gene-associated single nucleotide polymorphisms, individual marine fish can be assigned back to population of origin with unprecedented high levels of precision. By applying high differentiation single nucleotide polymorphism assays, in four commercial marine fish, on a pan-European scale, we find 93-100% of individuals could be correctly assigned to origin in policy-driven case studies. We show...
Tipo: Text
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00090/20085/17722.pdf

In vitro proteolysis of myofibrillar and sarcoplasmic proteins of white muscle of sea bass (Dicentrarchus labrax L.): effects of cathepsins B, D and L
Provedor de dados: 5
Autores: Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; Noel, Joelle; Fleurence, Joel.
The purpose of this study was to obtain additional information regarding proteolysis mechanisms and disorganization of fish myofibrils resulting in a loss of flesh quality. The ability of cathepsins to degrade in vitro myofibrillar and sarcoplasmic proteins from fish muscle was investigated in order to explain their role in post mortem softening. This led to the identification of substrates of the enzymes. Cathepsins degraded myosin heavy chain and a-actinin. Tropomyosin and actin were only susceptible to the action of cathepsin L. Troponin T (assumed 32 kDa component) was resistant only to the action of cathepsin D. Desmin was degraded by cathepsins B and L. Slight changes of some other myofibrillar or cytosolic proteins were also observed (creatine...
Tipo: Text
Palavras-chave: Post mortem degradation; Fish muscle proteins; Cathepsins; In vitro proteolysis.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-1684.pdf

Effect of high pressure on the calpain-calpastatin system in fish muscle
Provedor de dados: 5
Autores: Cheret, Romuald; Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; De Lamballerie, Marie.
Calpains (calcium-activated neutral proteases) of sea bass (Dicentrarchus labrax L.) muscle may participate in the degradation of muscle tissue during postmortem storage. These enzymes are regulated by calpastatin, their endogenous specific inhibitor. The objective of this study was to evaluate the changes encountered by the calpain system during the postmortem storage of fish muscle after high-pressure treatment. From 100 MPa, high-pressure treatment of purified calpains results in a loss of their activity as well as in the dissociation of the heterodimeric form. In muscle, the high-pressure processing decreases the initial activity of calpain. This loss in activity may be due to an inactivation by a change of structure. Initial calpastatin activity is...
Tipo: Text
Palavras-chave: Postmortem denaturation; High pressure; Fish; Calpain system.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2786.pdf

Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France
Provedor de dados: 5
Autores: Certad, Gabriela; Follet, Jérôme; Gantois, Nausicaa; Hammouma-ghelboun, Ourida; Guyot, Karine; Benamrouz-vanneste, Sadia; Fréalle, Emilie; Seesao, Yuwalee; Delaire, Baptiste; Creusy, Colette; Even, Gaël; Verrez-bagnis, Veronique; Ryan, Una; Gay, Mélanie; Aliouat-denis, Cécile; Viscogliosi, Eric.
Cryptosporidium, a zoonotic pathogen, is able to infect a wide range of hosts including wild and domestic animals, and humans. Although it is well known that some parasites are both fish pathogens and recognized agents of zoonosis with a public health impact, little information is available concerning the prevalence of Cryptosporidium in wild aquatic environments. To evaluate the prevalence of Cryptosporidium spp. in commercially important edible marine fish in different European seas (English channel, North sea, Bay of Biscay, Celtic sea and Mediterranean sea), 1,853 specimens were collected as part of two surveys. Nested PCR followed by sequence analysis at the 18S rRNA gene locus was used to identify Cryptosporidium spp. The overall prevalence of...
Tipo: Text
Palavras-chave: Cryptosporidium; Edible marine fish; 18S rRNA gene; Gp60; Molecular epidemiology; Phylogeny; Novel genotypes; European seas.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00497/60864/64198.pdf

Evaluation, signalling and willingness to pay for traceability. A cross-national comparison
Provedor de dados: 5
Autores: Calvo Dopico, D.; Mendes, R.; Silva, H.a.; Verrez-bagnis, Veronique; Pérez-martín, R.; Sotelo, C.g.
This study aims to investigate the benefits associated with traceability and its relationship to quality. Market research has been conducted in Spain, Portugal, France, UK and Germany. The results show that the expected benefits for consumers related to traceability focus on food safety and quality. Relevant information about traceability should focus on the intrinsic quality attributes highly valued by consumers and food safety. Among them, it highlights the origin, the value of which has been highly appreciated by most consumers in different countries. As regards signalling, consumers rate labelling very favourably, which would have to offer all relevant information on the intrinsic quality attributes. The implementation of technology (e.g. QR) could...
Tipo: Text
Palavras-chave: Labelling; Signal; Consumer behaviour; Traceability; Quality.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00349/46017/46901.pdf

Development of a qPCR Method for the Identification and Quantification of Two Closely Related Tuna Species, Bigeye Tuna (     Thunnus obesus     ) and Yellowfin Tuna (     Thunnus albacares     ), in Canned Tuna
Provedor de dados: 5
Autores: Bojolly, Daline; Doyen, Perine; Le Fur, Bruno; Christaki, Urania; Verrez-bagnis, Veronique; Grard, Thierry.
Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one...
Tipo: Text
Palavras-chave: Tuna; Authentication; Quantification; Canned products; TaqMan; QPCR; Species identification; Bigeye tuna (Thunnus obesus); Yellowfin tuna (Thunnus albacares).
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00371/48217/48403.pdf

In vitro proteolysis of myofibrillar and sarcoplasmic proteins of European sea bass (Dicentrarchus Labrax L) by an endogenous m-calpain
Provedor de dados: 5
Autores: Verrez-bagnis, Veronique; Delbarre Ladrat, Christine; Noel, Joelle; Fleurence, Joel.
The effects of m-calpain isolated from the skeletal muscle of sea bass on sarcoplasmic and myofibrillar proteins isolated from the same tissue were examined in vitro. Incubation of sarcoplasmic proteins with m-calpain resulted in only a slight decrease (0.7 kDa) in the molecular weight (MW) of a 26.5 kDa protein. Degradation of myofibrils, monitored by quantification of TCA-soluble peptides generated, resulted in the maximum amount of peptides being generated after 1 h of incubation at 25degreesC. Noticeable modifications in the SDS-PAGE profile of digested myofibrils were observed, including partial denaturation of myosin heavy chain and the release of tropomyosin, similar to69 and similar to27 kDa doublet bands and a few polypeptides of MW lower than 20...
Tipo: Text
Palavras-chave: Dicentrarchus labrax L; Proteolysis; Sarcoplasmic protein; Calpain; Myofibrillar protein.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-1108.pdf

Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes
Provedor de dados: 5
Autores: Seesao, Y.; Audebert, C.; Verrez-bagnis, Veronique; Merlin, S.; Jerome, Marc; Viscogliosi, E.; Dei-cas, E.; Aliouat-denis, C. M.; Gay, Melanie.
Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions.
Tipo: Text
Palavras-chave: Anisakidae; High-throughput sequencing; Nematode; Pooled DNA extraction; Marine fish; COX2.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00189/30020/28607.pdf

Identification of skipjack tuna juveniles based on DNA control region sequences and potential spawning area around reunion island
Provedor de dados: 5
Autores: Nikolic, Natacha; Jerome, Marc; Fonteneau, Alain; Evano, Hugues; Verrez-bagnis, Veronique.
In this study, we confirmed that DNA control region can be used to identify potential spawning area from pelagic juveniles species. We used the mitochondrial DNA control region to identify tuna juveniles predated by common dolphinfish and sampled in surface waters around Reunion Island. These were skipjack juveniles at an early stage (small length and young age). The biological results confirmed the species identification based on DNA analysis. Catch data by longline reveal a higher number of skipjack from October to December around Reunion Island corresponding to the period of our skipjack juvenile sampling
Tipo: Text
Palavras-chave: Skipjack tuna; Reunion island; DNA; Age; Juvenile.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00299/40993/42657.pdf

Genetic catalogue, biological reference collections and online database of European marine fishes
Provedor de dados: 5
Autores: Bautista, José M.; Verrez-bagnis, Veronique; Kourti, Naouma.
Taxonomy and systematics of most animal species have been described in sufficient detail to permit the classification of practically any organism and, in particular, may be of use in the identification of the species and subspecies for the fish trade and ecological concerns regarding fisheries. However, before FishTrace started there was a lack of fast reference tools that hindered the efficient identification and differentiation of teleosts required in fisheries management, biological and ecological research, and human consumption. This problem could affect several of the socio-economic activities related to Fisheries Policies and therefore, the constitution of a database for identification of fish species of commercial, ecological and zoological interest...
Tipo: Text
Palavras-chave: Database; Fish; Genetics.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/rapport-6595.pdf

Maîtrise de la texture des produits à base de surimi
Provedor de dados: 5
Autores: Han Ching, Lucay; Verrez-bagnis, Veronique.
Le surimi est une fraction protéique stabilisée, obtenue a partir de muscles de poissons. II peut être fabriqué dans des usines à bord des bateaux ou à terre.
Tipo: Text
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00057/16809/14272.pdf

Essai de certaines combinaisons de cryoprotecteurs additionnées à la pulpe de cabillaud congelé, après récupération mécanique à partir des chutes de filetage
Provedor de dados: 5
Autores: Verrez-bagnis, Veronique.
La récupération mécanique de pulpe de poisson à partir des résidus de filetage permet d’augmenter de 6 à 10 % le rendement en chair utilisable du cabillaud Cadus morhua. Mais pendant l'entreposage congelé, les proteines de la pulpe se dénaturent très rapidement, aussi l'emploi de substances (qu'on appelle cryoprotecteurs) qui ralentiraient, retarderaient ou inhiberaient partiellement la dénaturation des protéines a-t-il été envisagé. Afin de profiter des divers mécanismes d'action de chaque agent, il a paru intéressant de tester des mélanges de substances cryoprotectrices.
Tipo: Text
Ano: 1984
URL: http://archimer.ifremer.fr/doc/00137/24868/22956.pdf

Trends in postmortem aging in fish: Understanding of proteolysis and disorganization of the myofibrillar structure
Provedor de dados: 5
Autores: Delbarre Ladrat, Christine; Cheret, Romuald; Taylor, Romuald; Verrez-bagnis, Veronique.
Introduction :  Quality attributes of fish flesh, including food safety, organoleptic features, nutritional quality and aptitude to industrial transformation, influence consumption and acceptability of fish as food. Fish sensorial changes and texture properties are closely linked to freshness. Along with ante mortem muscle biochemistry, postmortem biochemical processes are directly linked to final quality attributes. The understanding of postmortem mechanisms is a prerequisite for an accurate control of the quality of commercialized fish by the identification of objective markers or indicators. Postmortem tenderization is one of the most important quality attributes of fish muscle. Loss of freshness is due to a complex combination of biochemical, chemical...
Tipo: Text
Palavras-chave: Indicator; Quality; Proteolysis; Postmortem; Fish muscle.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1810.pdf

Current Methods for Seafood Authenticity Testing in Europe: is there a Need for Harmonisation?
Provedor de dados: 5
Autores: Griffiths, Andrew M.; Sotelo, Carmen G.; Mendes, Rogerio; Perez-martin, Ricardo I.; Schroeder, Ute; Shorten, Marc; Silva, Helena A.; Verrez-bagnis, Veronique; Mariani, Stefano.
Mislabelling of food products has recently received a great deal of public scrutiny, but it remains unclear exactly what methods are being utilised in laboratories testing the authenticity of foods. In order to gain insight into the specific area of the analysis of seafood, a questionnaire focusing on the taxonomic groups typically analysed and the techniques utilised was sent to over one hundred accredited laboratories across the UK, Ireland, Spain, Portugal, France and Germany. Forty-five responded positively, demonstrating significant differences in both the species analysed and methods utilised among the countries included in the survey. Indeed, a diversity of methods was employed across laboratories and efforts to harmonise and/or standardise testing...
Tipo: Text
Palavras-chave: Food testing; Forensically informative nucleotide sequencing; Species identification; DNA barcoding; Fisheries.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00188/29918/28383.pdf

Tuna labels matter in Europe: Mislabelling rates in different tuna products
Provedor de dados: 5
Autores: Sotelo, Carmen G.; Velasco, Amaya; Perez-martin, Ricardo, I; Kappel, Kristina; Schroeder, Ute; Verrez-bagnis, Veronique; Jerome, Marc; Mendes, Rogerio; Silva, Helena; Mariani, Stefano; Griffiths, Andrew.
Tuna fisheries and processing represent economic activities of paramount importance around the world. Most of these products are traded for human consumption and in general are highly demanded commodities. However, not all tuna products achieve the same market price, some consumers are willing to pay a huge amount of money for certain species (i.e. Japanese market for Bluefin tuna) while other species are rather affordable (i.e. Skipjack tuna), therefore mislabelling has been observed frequently. We collected and analysed 545 tuna samples in six European countries, including fresh, frozen and canned products, and we have investigated whether or not these products were correctly labelled under European and national legislations. We found an overall...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00440/55137/56604.pdf

Restriction fragment length analysis of the cytochrome b gene and muscle fatty acid composition differentiate the cryptic flatfish species Solea solea and Solea aegyptiaca
Provedor de dados: 5
Autores: Boukouvala, Evridiki; Cariani, Alessia; Maes, Gregory E.; Sevilla, Rafael G.; Verrez-bagnis, Veronique; Jerome, Marc; Guarniero, Ilaria; Monios, Georgios; Tinti, Fausto; Volckaert, Filip A.m.; Bautista, José M.; Krey, Grigorios.
Overlapping external morphometric characters easily confound the flatfishes Solea aegyptiaca and Solea solea (Soleidae) in areas of the Mediterranean Sea where both species live in sympatry. This leads to uncertainties in the fisheries and marketing of the species, in addition to misinterpretations in biogeography and conservation studies. Here we describe a simple restriction fragment length-based diagnostic test, which differentiates S. solea from S. aegyptiaca, as well as from other species of the Soleidae family. Furthermore, we show that the two species living in sympatry in the Gulf of Kavala (North Aegean Sea, Greece) present significant qualitative differences in muscle fatty acid composition, a property which can also be used to distinguish the...
Tipo: Text
Palavras-chave: Solea solea; Solea aegyptiaca; Species diagnosis; Cytochrome b gene; EcoR V restriction fragment length; White muscle fatty acid composition.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00088/19974/17641.pdf

Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae
Provedor de dados: 5
Autores: Lebellenger, Lou; Verrez-bagnis, Veronique; Passerini, Delphine; Delbarre Ladrat, Christine.
Objectives The eps locus in Vibrio diabolicus is involved in the production of the biotechnologically valuable HE800 EPS. In this study, the distribution and diversity of similar eps gene clusters across Vibrionaceae and its variability in relation to phylogenetic relationship were investigated. The aim was to provide a better knowledge of the eps gene cluster importance and to facilitate discovery of new EPS with potent interesting bioactivities. Results Seventy percent of the 103 genome sequences examined display such an eps locus with a high level of synteny. However, genetic divergence was found inside some monophyletic clades or even between some strains of the same species. It includes gene insertions, truncations, and deletions. Comparative analysis...
Tipo: Text
Palavras-chave: Vibrionaceae; Exopolysaccharide; Genetic biodiversity; Biosynthesis; Vibrio diabolicus; HE800 EPS.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00424/53536/54392.pdf

Low mislabeling rates indicate marked improvements in European seafood market operations
Provedor de dados: 5
Autores: Mariani, Stefano; Griffiths, Andrew M.; Velasco, Amaya; Kappel, Kristina; Jerome, Marc; Perez-martin, Ricardo I.; Schroeder, Ute; Verrez-bagnis, Veronique; Silva, Helena; Vandamme, Sara G.; Boufana, Belgees; Mendes, Rogerio; Shorten, Marc; Smith, Cat; Hankard, Elizabeth; Hook, Samantha A.; Weymer, Alice S.; Gunning, Daryl; Sotelo, Carmen G.
Over the span of a decade, genetic identification methods have progressively exposed the inadequacies of the seafood supply chain, revealing previously unrecognized levels of seafood fraud, raising awareness among the public, and serving as a warning to industry that malpractice will be detected. Here we present the outcome of the latest and largest multi-species, transnational survey of fish labeling accuracy to date, which demonstrates an apparent sudden reduction of seafood mislabeling in Europe. We argue that recent efforts in legislation, governance, and outreach have had a positive impact on industry regulation. Coordinated, technology-based, policy-oriented actions can play a pivotal role in shaping a transparent, sustainable global seafood market...
Tipo: Text
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00302/41323/40517.pdf

Propriétés gélifiantes des protéines myofibrillaires du poisson (surimi). L'exemple du Tacaud (Trisopterus luscus)
Provedor de dados: 5
Autores: Verrez-bagnis, Veronique; Chopin, Christine; Han Ching, Lucay.
Surimi is a japanese term refering to minced and thoroughly washed fish meat. Quality of surimi is determined by its ability to form a firm and elastic gel after heat treatment. The parameters affecting this property are : fish species, storage before further processing (refrigeration, icing, freezing), addition of cryoprotectors limiting the protein denatura during freeze storage, addition of texturing agent such as starch and egg white. The effects of some of these parameters have been studied on Whiting-pout surimi (Trisoptesus Luscus). Water content of surimi has a great influence on kamaloko quality. Increase ,in starch and egg white concentration does not improve gel quality in terms of rigidity and cohesiveness. A ten day storage of whiting-pout on...
Tipo: Text
Ano: 1988
URL: http://archimer.ifremer.fr/doc/00057/16808/14268.pdf

Petromyzon marinus (Petromyzontidae), an unusual host for helminth parasites in western Europe
Provedor de dados: 5
Autores: Gerard, Claudia; Verrez-bagnis, Veronique; Jerome, Marc; Lasne, Emilien.
The sea lamprey Petromyzon marinus, which is among the most phylogenetically ancient vertebrates, is a hematophagous ectoparasite that feeds on vertebrates and is considered vulnerable in Europe but is a pest in the North American Great Lakes. We conducted a literature review of helminth parasites of Petromyzon marinus and investigated postmetamorphic lampreys sampled in rivers and northeast Atlantic coastal waters (western France) during spawning migration. Based on the literature review, 16 helminth taxa have been recorded in Petromyzon marinus, among them 14 in North America but only 2 in Europe, with no species in common between these areas. Specific parasites are lacking, and helminth parasites recorded in Petromyzon marinus are mostly opportunistic...
Tipo: Text
Palavras-chave: Sea lamprey; Helminth parasites; Unusual host; Accidental ingestion; Anisakis simplex sensu strict; Nematode.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00268/37964/36040.pdf

PCR-RFLP analyses of formalin-fixed fish eggs for the mapping of spawning areas in the Eastern Channel and Southern North Sea
Provedor de dados: 5
Autores: Lelievre, Stephanie; Verrez-bagnis, Veronique; Jerome, Marc; Vaz, Sandrine.
A better knowledge on spawning grounds of principal commercial fishes from North Sea is necessary for their monitoring. Fish egg taxonomic identification based on morphological characters cannot always be carried out effectively. This is particularly the case for a number of Gadidae fish species eggs (i.e. cod, haddock and whiting, and three rockling species) which have the same range of size and for which molecular-based techniques can help improving the accuracy of egg taxonomic identification, and hence the reliability of studies on spawning areas. In this study, formalin fixation, egg conservation and DNA extraction were optimized in order to develop a specific PCR-RFLP method. Based on the sequence of the cytochrome b gene of reference specimens, a...
Tipo: Text
Palavras-chave: Fish eggs; PCR-RFLP; Cytochrome b gene; Geostatistical analyses; Interpolated map.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00005/11613/8259.pdf

Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds
Provedor de dados: 5
Autores: Lelievre, Stephanie; Jerome, Marc; Maes, Gregory E.; Vaz, Sandrine; Calaivany, Sachidhanandam; Verrez-bagnis, Veronique.
Maps of the spawning grounds of commercially important fishes are necessary when assessing the level of connectivity between life stages of fishes and for identifying ecologically valuable marine areas. A first step toward mapping the spawning grounds is a reliable and rapid species identification of pelagic fish eggs to assess the spatio-temporal distribution of spawning aggregations. As many species have similar egg sizes and morphology, the molecular validation of visually identified eggs is often essential for the use of such data in fisheries management. In the present study, we developed a rapid 16S rRNA PCR-restriction fragment length polymorphism (RFLP) assay to distinguish between formalin-fixed fish eggs of dab Limanda limanda, flounder...
Tipo: Text
Palavras-chave: Fish eggs; PCR-RFLP; 16S rRNA; Geostatistical analyses; Distribution; Spawning grounds.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00059/16999/14536.pdf

Suite de l'étude entreprise sur la texture du surimi et gel de surimi (Kamaboko) de chinchard (Trachurus trachurus)
Provedor de dados: 5
Autores: Verrez-bagnis, Veronique.
A la question : Qu'est-ce que le surimi ?, l'introduction du rapport annuel d'activité de juin 1985 "Essais préliminaires et premleres études de l'influence des paramètres technologiques sur la texture du surimi et gel de surimi de chinchard (Trachurus trachurus)" a tenté d'apporter une réponse.  Après le détail de la fabrication du surimi employée ainsi que les résultats de quelques essais préliminaires permettant de choisir les méthodes de mesures adéquates, nous avions abordé la premiere grande partie du travail - l'optimisation des conditions technologiques - et indiqué les prémices d'une étude sur la microstructure du surimi et du kamaboko.  Actuellement la première partie de l'étude est terminée et deux articles "Texture du kamaboko de chinchard...
Tipo: Text
Ano: 1986
URL: http://archimer.ifremer.fr/doc/00125/23671/21520.pdf

Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes
Provedor de dados: 5
Autores: Sevilla, Rafael G.; Diez, Amalia; Noren, Michael; Mouchel, Olivier; Jerome, Marc; Verrez-bagnis, Veronique; Van Pelt, Hilde; Favre Krey, Laurence; Krey, Grigorios; Bautista, José M.
This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.
Tipo: Text
Palavras-chave: Teleost; Rhodopsin; PCR primers; Mitochondrial cytochrome b; Fish; DNA barcoding.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3034.pdf