Consulta: creatorFacets:"Walia,Abhishek"
Registros recuperados: 2
Data/hora: 05/06/2024 07:56:06

Molecular Cloning and Sequencing of AlkalophilicCellulosimicrobium cellulans CKMX1 Xylanase Gene Isolated from Mushroom Compost and Characterization of the Gene Product
Provedor de dados: 52
Autores: Walia,Abhishek; Mehta,Preeti; Guleria,Shiwani; Chauhan,Anjali; Shirkot,C.K.
ABSTRACT A xylanolytic bacterium was isolated from mushroom compost by using enrichment technique. Results from the metabolic fingerprinting, whole-cell fatty acids methyl ester analysis and 16S rDNA sequencing suggested the bacterium to be Cellulosimicrobium cellulans CKMX1. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanase gene were attempted. A distinct fragment of about 1671 bp was successfully amplified using PCR and cloned into Escherichia coli DH5α. A BLAST search confirmed that the DNA sequence from the amplified fragment was endo-1, 4-beta-xylanase, which was a member of glycoside hydrolase family 11. It showed 98% homology withCellulosimicrobium sp. xylanase gene (Accession no. FJ859907.1) reported...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellulase free xylanase; Cellulosimicrobium cellulans; E.coli; Cloning; Gene.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600913

Tricalcium phosphate solubilization and nitrogen fixation by newly isolated Aneurinibacillus aneurinilyticus CKMV1 from rhizosphere of Valeriana jatamansi and its growth promotional effect
Provedor de dados: 58
Autores: Chauhan,Anjali; Guleria,Shiwani; Balgir,Praveen P.; Walia,Abhishek; Mahajan,Rishi; Mehta,Preeti; Shirkot,Chand Karan.
Abstract Aneurinibacillus aneurinilyticus strain CKMV1 was isolated from rhizosphere of Valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260 mg/L), nitrogen fixation (202.91 nmol ethylene mL-1 h-1), indole-3-acetic acid (IAA) (8.1 µg/mL), siderophores (61.60%), HCN (hydrogen cyanide) production and antifungal activity. We investigated the ability of isolate CKMV1 to solubilize insoluble P via mechanism of organic acid production. High-performance liquid chromatography (HPLC) study showed that isolate CKMV1 produced mainly gluconic (1.34%) and oxalic acids. However, genetic evidences for nitrogen fixation and phosphate solubilization by organic acid production have been reported first time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Valeriana jatamansi; Phosphate solubilization; Nitrogen fixation; Glucose dehydrogenase; Aneurinibacillus aneurinilyticus; PGPR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200294