Consulta: creatorFacets:"Wang,Xingjun"
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Data/hora: 02/06/2024 14:39:53

Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene
Provedor de dados: 69
Autores: Zhang,Yue; Xia,Han; Yuan,Mei; Zhao,Chuanzhi; Li,Aiqin; Wang,Xingjun.
Chalcone isomerase (CHI) is the key enzyme that catalyzes chalcone into (2S)-flavanol or (2S)-5-desoxidation flavanol. The full length cDNA (1050 bp) of AhCHI (Arachis hypogaea CHI gene) was cloned by large scale EST sequencing using a peanut immature seed cDNA library. Sequence analysis results indicated that it was a type I CHI gene (with the accession number JN660794). The ORF of AhCHI was 768 bp, encoding a peptide of 255 amino acids with a pI of 5.189. Sequence alignment showed that the coding region of AhCHI gene is highly conserved to compare with CHI genes from other plant species. Peanut cDNA microarray and semi-quantitative RT-PCR analysis indicated that AhCHI was highly expressed in pegs. The expression level in flower and root was higher than...
Tipo: Journal article
Palavras-chave: Arachis hypogaea L.; CDNA library; Chalcone isomerase; Expression analysis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100005