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A new, harmless, high-throughput endosperm-based DNA extraction method for wheat
Provedor de dados: 65
Autores: Ma,Zhihui; Wang,Yuquan; Wei,Wenhui; Ru,Zhengang.
ABSTRACT:  In this study, a non-destructive, high-throughput, endosperm-based DNA extraction method was developed. To verify the non-destructive nature of this method, a germination test was performed on 288 seeds after sampling their endosperm, which gave a seedling emergence rate that was higher (97.6%) than that of the control group (92%). To confirm the feasibility of the new method, DNA was extracted from plants of a BC1F2 population by two different methods, namely, from endosperm using our rapid, high-throughput method (ER-DNA) and from young leaves emerging from the same sampled seed using the CTAB method (LC-DNA). The ER-DNA was undetectable by agarose gel electrophoresis, but was found to be an adequate replacement for LC-DNA for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Triticum aestivum; SSR; Segregation population.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000900200