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Influence of the pH of glutaraldehyde and the use of dextran aldehyde on the preparation of cross-linked enzyme aggregates (CLEAs) of lipase from Burkholderia cepacia
Provedor de dados: 69
Autores: Caballero Valdés,Eduardo; Wilson Soto,Lorena; Aroca Arcaya,Germán.
The preparation of cross-linked enzyme aggregates (CLEAs) of lipase has been a challenge due the low amount of lysine residues that lipases have on their surface. The results show that CLEAs prepared using dextran aldehyde (100-200KDa) have a higher hydrolysis activity and particle size (activities between 3186 Â± 21 U/g of CLEA and 4800 Â± 30 U/g of CLEA and particle sizes between 52.6 Â± 18.7 Âµm and 126.2 Â± 53.5 Âµm) than CLEAs prepared with glutaraldehyde (0.1 KDa) (activities between 894 Â± 16 U/g of CLEA and 2874 Â± 20 U/g of CLEA and particle sizes between 21.2 Â± 5.1 Âµm and 83.4 Â± 24.9 Âµm); Thermal stability assays of bioctalysts at 60ÂºC at pH 7.0 using phosphate buffer 25 mM showed that CLEAs prepared with dextran aldehyde have lower residual...
Tipo: Journal article
Palavras-chave: Albumin; Dextran aldehyde; Glutaraldehyde; Immobilisation; Lipase.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300010