Consulta: creatorFacets:"Womack,J.E."
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A methodological approach for the construction of a radiation hybrid map of bovine chromosome 5
Provedor de dados: 74
Autores: De Donato,M.; Brenneman,R.A.; Stelly,D.M.; Womack,J.E.; Taylor,J.F.
A bovine 5,000 rad WG-RH panel was used to construct an RH map of bovine chromosome 5 (BTA5). Twenty-one microsatellites and thirteen genes were scored in the panel using PAGE and radioactive labeling. Marker retention ranged from 8.9%-25.8% and averaged 17.8%. Pairwise locus analysis placed all markers in a single syntenic group with a LOD support of 4.0. At a LOD support of 8.0, a centromeric group of 23 syntenic markers was formed. Telomeric groups of 11 and 9 markers were assembled with a LOD support of 6.0 and 8.0, respectively. All markers were ordered by maximum likelihood methods using the program RHMAP. Only 13 markers were ordered with a LOD support of at least 3.0, while 25 and 29 markers were ordered with a support of at least 2.0 and 1.0,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RH map; Bovine; Gene mapping.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100005