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In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens
Provedor de dados: 89
Autores: Zimmermann,CEP; Machado,AK; Cadoná,FC; Jaques,JAS; Schlemmer,KB; Lautert,C; Cruz,IBM; Zanette,RA; Leal,DBR; Santurio,JM.
The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p &lt; 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p &lt; 0.01 and p &lt; 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p &lt; 0.001). ROS formation significantly increased at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxin B1; Broilers; Lymphocytes; PicoGreen; Reactive oxygen species.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300012