Consulta: creatorFacets:"Zhang,Zhen"
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Data/hora: 15/06/2024 16:12:33

Evaluation of Reference Genes for Quantitative PCR in Four Tissues from Rabbits with Hypercholesterolaemia
Provedor de dados: 52
Autores: Zhang,Zhen; Wen,Bin; Xu,Yuan; Jiang,En-ze; Liu,Jia-yu; Zhu,Ke-li; Ning,Fang-yong; Du,Zhi-Heng; Bai,Xiu-Juan.
Abstract Rabbit with hypercholesterolaemia is an important model for studying cholesterol metabolism disease. This study aimed to evaluate the expression stability of nine reference genes for quantitative PCR (qPCR) analysis in adrenal gland, liver, spleen, and kidney tissue from rabbits with hypercholesterolaemia. In total, 30 male Harbin Large White (HLW) rabbits were fed a normal feed (n = 15) or a high cholesterol feed (n = 15) for 8 weeks to induce hypercholesterolaemia. Nine reference genes were verified by qPCR using cDNA extracted from rabbit tissue samples. For qPCR analysis, reference genes were evaluated using the RefFinder and GeNorm algorithms. Overall, seven rabbits with hypercholesterolaemia were identified based on body weight and total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Evaluation; Reference genes; QPCR; Rabbits; Hypercholesterolaemia.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100428

Mask-wearing and respiratory infection in healthcare workers in Beijing, China
Provedor de dados: 55
Autores: Yang,Peng; Seale,Holly; MacIntyre,C Raina; Zhang,Haiyan; Zhang,Zhen; Zhang,Yi; Wang,Xiaoli; Li,Xinyu; Pang,Xinghuo; Wang,Quanyi.
OBJECTIVES: The aim of the study was to determine rates of mask-wearing, of respiratory infection and the factors associated with mask-wearing and of respiratory infection in healthcare workers (HCWs) in Beijing during the winter of 2007/2008. METHODS: We conducted a survey of 400 HCWs working in eight hospitals in Beijing by face to face interview using a standardized questionnaire. RESULTS: We found that 280/400 (70.0%) of HCWs were compliant with mask-wearing while in contact with patients. Respiratory infection occurred in 238/400 (59.5%) subjects from November, 2007 through February, 2008. Respiratory infection was higher among females (odds ratio [OR], 2.00 [95% confidence interval {CI}, 1.16-3.49]) and staff working in larger hospitals (OR, 1.72...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Masks; Respiratory tract infections; Health personnel.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200002

A high-carbohydrate diet enhances the adverse effect of the S2 allele of APOC3 SstI polymorphism on the TG/HDL-C ratio only in young Chinese females
Provedor de dados: 56
Autores: Song,Yong Yan; Gong,Ren Rong; Zhang,Zhen; Li,Yuan Hao; Xiao,Li Ying; Zhou,Xue Dong; Fang,Ding Zhi.
Both genetic background and diet have profound effects on plasma lipid profiles. We hypothesized that a high-carbohydrate (high-CHO) diet may affect the ratios of serum lipids and apolipoproteins (apo) differently in subjects with different genotypes of the SstI polymorphism in the apoCIII gene (APOC3). Fifty-six healthy university students (27 males and 29 females, 22.89 ± 1.80 years) were given a washout diet of 54% carbohydrate for 7 days, followed by a high-CHO diet of 70% carbohydrate for 6 days without total energy restriction. Serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apoB100, apoAI, and the APOC3 SstI polymorphism were analyzed. The ratios of serum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-carbohydrate diet; Lipid ratio; Gene polymorphism; Human APOC3.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600005