Consulta: subjectFacets:"16S"
Registros recuperados: 9
Data/hora: 01/06/2024 18:07:59

Leaf-associated bacterial microbiota of coffee and its correlation with manganese and calcium levels on leaves
Provedor de dados: 74
Autores: Sousa,Leandro Pio de; Silva,Marcio José da; Costa Mondego,Jorge Maurício.
Abstract Coffee is one of the most valuable agricultural commodities and the plants’ leaves are the primary site of infection for most coffee diseases, such as the devastating coffee leaf rust. Therefore, the use of bacterial microbiota that inhabits coffee leaves to fight infections could be an alternative agricultural method to protect against coffee diseases. Here, we report the leaf-associated bacteria in three coffee genotypes over the course of a year, with the aim to determine the diversity of bacterial microbiota. The results indicate a prevalence of Enterobacteriales in Coffea canephora, Pseudomonadales in C. arabica ‘Obatã’, and an intriguing lack of bacterial dominance in C. arabica ‘Catuaí’. Using PERMANOVA analyses, we assessed the association...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Bacteria; 16S; Leaf; Manganese; Calcium.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300455

Presence of two mitochondrial genomes in the mytilid Perumytilus purpuratus: Phylogenetic evidence for doubly uniparental inheritance
Provedor de dados: 74
Autores: Vargas,Jaime; Pérez,Montse; Toro,Jorge; Astorga,Marcela P.
This study presents evidence, using sequences of ribosomal 16S and COI mtDNA, for the presence of two mitochondrial genomes in Perumytilus purpuratus. This may be considered evidence of doubly uniparental mtDNA inheritance. The presence of the two types of mitochondrial genomes differentiates females from males. The F genome was found in the somatic and gonadal tissues of females and in the somatic tissues of males; the M genome was found in the gonads and mantle of males only. For the mitochondrial 16S region, ten haplotypes were found for the F genome (nucleotide diversity 0.004), and 7 haplotypes for the M genome (nucleotide diversity 0.001), with a distance Dxy of 0.125 and divergence Kxy of 60.33%. For the COI gene 17 haplotypes were found for the F...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mytilidae; 16S; COI; Perumytilus; DUI.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200173

Identification and characterisation of winter spawning ground in the English Channel and Southern North Sea
Provedor de dados: 5
Autores: Lelievre, Stephanie.
A better knowledge and monitoring of principal commercial fish spawning grounds have become necessary in the North Sea. The efficiency of CUFES was proved by sampling pelagic fish eggs in winter in Eastern Channel and Southern North Sea. Fish egg taxonomic identification based on visual criteria cannot always be carried out effectively. In particular, cod (Gadus morhua), and whiting (Merlangius merlangus) or flounder (Platichthys flesus) and dab (Limanda limanda) have the same range of egg diameter and similar morphologies. Alternative identification methods using molecular techniques were developed to improve the accuracy of egg taxonomic identification. First, PCR-RFLP method, then, in order to accelerate egg identification, the use of a new laboratory...
Tipo: Text
Palavras-chave: Oeufs de poissons; CUFES; VET; PCR-RFLP; Cyt b; 16S; ZooScan; Analyse d’images; Analyses géostatistiques; Interpolation; Modélisation d’habitat; GLM; RQ; Fish eggs; CUFES; VET; Image analysis; ZooScan; PCR-RFLP; Geostatistical analyses; Interpolated map; Habitat modelling; GLM; RQ.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00043/15398/12757.pdf

Selective elimination of chloroplastidial DNA for metagenomics of bacteria associated with the green alga Caulerpa Taxifolia (bryopsidophyceae)
Provedor de dados: 5
Autores: Aires, Tania; Marba, Nuria; Serrao, Ester A.; Duarte, Carlos M.; Arnaud-haond, Sophie.
Molecular analyses of bacteria associated with photosynthetic organisms are often confounded by coamplification of the chloroplastidial 16S rDNA with the targeted bacterial 16S rDNA. This major problem has hampered progress in the characterization of bacterial communities associated to photosynthetic organisms and has limited the full realization of the potential offered by the last generation of metagenomics approaches. A simple and inexpensive method is presented, based on ethanol and bleach treatments prior to extraction, to efficiently discard a great part of chloroplastidial DNA without affecting the characterization of bacterial communities through pyrosequencing. Its effectiveness for the description of bacterial lineages associated to the green...
Tipo: Text
Palavras-chave: 16S; Chloroplasts; Elimination; Green algae; Metagenomics.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00076/18739/16464.pdf

Molecular species delimitation methods and population genetics data reveal extensive lineage diversity and cryptic species in Aglaopheniidae (Hydrozoa)
Provedor de dados: 5
Autores: Postaire, Bautisse; Magalon, Helene; Bourmaud, Chloe A. -f.; Bruggemann, J. Henrich.
A comprehensive inventory of global biodiversity would be greatly improved by automating methods for species delimitation. The Automatic Barcode Gap Discovery method, the Poisson tree processes algorithm and the Generalized mixed Yule-coalescent model have been proposed as means of increasing the rate of biodiversity description using single locus data. We applied these methods to explore the diversity within the Aglaopheniidae, a hydrozoan family with many species widely distributed across tropical and temperate oceans. Our analyses revealed widespread cryptic diversity in this family, almost half of the morpho-species presenting several independent evolutionary lineages, as well as support for cases of synonymy. For two common species of this family,...
Tipo: Text
Palavras-chave: Automatic Barcode Gap Discovery; Generalized mixed Yule-coalescent model; Poisson tree processes; 16S; Microsatellites; Cnidarians.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00348/45907/45590.pdf

Phylogeny and phylogeography of Atlantic oyster species: evolutionary history, limited genetic connectivity and isolation by distance
Provedor de dados: 5
Autores: Lazoski, C.; Gusmao, J.; Boudry, Pierre; Sole-cava, A. M.
The phylogenetic relationships between naturally occurring Atlantic Crassostrea oyster species were inferred through analyses of mitochondrial (cytochrome oxidase subunit I and 16S) and nuclear (second internal transcribed spacer) sequences. We also scored 15 allozyme loci on 422 oysters to study population structuring of C. rhizophorae and C. brasiliana along 9000 km of the Western Atlantic coastline. Despite morphological similarities, C. virginica was genetically more closely related to C. rhizophorae than to C. brasiliana. In contrast, C. paraibanensis was genetically indistinguishable from C. brasiliana, which is probably a junior synonym of the African C. gasar. Significant genetic differentiation between populations of C. rhizophorae and C. gasar...
Tipo: Text
Palavras-chave: Population genetics; Biogeography; Allozymes; Cytochrome c oxidase subunit I; COI; 16S; Internal transcribed spacer 2; ITS-2.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14464/11765.pdf

Co-occurring nematodes and bacteria in submarine canyon sediments
Provedor de dados: 5
Autores: Rzeznik-orignac, Jadwiga; Puisay, Antoine; Derelle, Evelyne; Peru, Erwan; Le Bris, Nadine; Galand, Pierre E.
In submarine canyon sediments, bacteria and nematodes dominate the benthic biomass and play a key role in nutrient cycling and energy transfer. The diversity of these communities remains, however, poorly studied. This work aims at describing the composition of bacteria and nematode communities in the Lacaze-Duthiers submarine canyon in the north-western Mediterranean Sea. We targeted three sediment depths for two consecutive years and investigated the communities using nuclear markers (18S rRNA and 16S rRNA genes). High throughput sequencing combined to maximal information coefficient (MIC) statistical analysis allowed us to identify, for the first time, at the same small scale, the community structures and the co-occurrence of nematodes and bacteria...
Tipo: Text
Palavras-chave: Bacteria; Meiofauna; Canyon Lacaze-Duthiers; Metabarcording; Molecular approach; Mediterranean Sea; Nematodes; Marine biodiversity; 18S; 16S.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00454/56586/76447.pdf

Historical biogeography of Western Palaearctic pelobatid and pelodytid frogs: a molecular phylogenetic perspective
Provedor de dados: 16
Autores: Veith, M.; Fromhage, L.; Kosuch, J.; Vences, M.
Spadefoot toads (Pelobates) and Parsley frogs (Pelodytes) are an enigmatic group of Western Palaearctic anurans. In the genus Pelobates, a fossorial lifestyle has enforced a conserved bauplan that masks their intraspecific evolutionary history. We used partial sequences of the mitochondrial 16S and 12S rRNA genes to infer a paleobiogeographic scenario of speciation events in these two anuran genera. Based on two alternative, mutually exclusive calibrations of the Iberian-African split within Pelobates (Pb. cultripes and Pb. varaldii), the disjunction of the Betic Cordillera ca. 14-16 million years ago (mya), and the end of the Messinian Salinity crisis 5.33 mya, we inferred alternative scenarios for species evolution within both genera applying...
Tipo: Article / Letter to the editor
Palavras-chave: Phylogeny; Paleobiogeography; Speciation; Regression-based molecular clock; Bayesian molecular dating; Messinian salinity crisis; 16S; 12S; 42.82.
Ano: 2006
URL: http://www.repository.naturalis.nl/record/214531

First case of Nocardia nova spinal abscess in an immunocompetent patient
Provedor de dados: 55
Autores: Hong,Seung Bok; Han,Kyudong; Son,Bo Ra; Shin,Kyeong Seob; Rim,Byeong Cheol.
Nocardia are a group of aerobic actinomycetes that are filamentous gram-positive, weakly acid-fast, and cause opportunistic infection in immunocompromised patients. Primary Nocardia infection mostly involves lung, skin and less commonly, the central nervous system (CNS). Among Nocardia CNS infections, spinal infection is extremely rare. We describe the first case of a spinal abscess caused by Nocardia nova in an immunocompetent patient who experienced a penetrating facial injury six months earlier. Nocardia species were isolated from intradural spinal abscesses and identified by 16S rRNA, hsp65 and secA1 sequence analyses. Surgical excision and treatment with amikacin, cefotaxime, and oral erythromycin was successful.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Nocardia Abscess Spine RNA; Ribosomal; 16S.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200017