Consulta: subjectFacets:"18S rRNA"
Registros recuperados: 4
Data/hora: 11/06/2024 07:04:06

Small-scale variability of protistan planktonic communities relative to environmental pressures and biotic interactions at two adjacent coastal stations
Provedor de dados: 5
Autores: Genitsaris, Savvas; Monchy, Sebastien; Breton, Elsa; Lecuyer, Eric; Christaki, Urania.
The aim of this study was to analyze planktonic protistan assemblages under different environmental pressures at 2 adjacent coastal stations (inshore and offshore; similar to 6.4 km apart) in the eastern English Channel (EEC). Samples were collected between March 2012 and June 2013, and analyzed using bioinformatic analysis and tag pyrosequencing of the V2-V3 hypervariable region of the 18S rRNA gene. In addition to the taxonomic composition of the protistan communities, the detected operational taxonomic units (OTUs) were sorted into 6 major functional groups based on their trophic roles in marine systems. Comparisons of 13 environmental factors, including physical and chemical variables, indicated significant differences between the 2 stations. However,...
Tipo: Text
Palavras-chave: Unicellular eukaryotes; Community organization; Environmental parameters; Biotic interactions; 18S rRNA; Pyrosequencing; Bioinformatics; Ecological tools.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00373/48450/48765.pdf

First detection of Babesia venatorum (EU1) in Ixodes persulcatus ticks in Mongolia
Provedor de dados: 19
Autores: Tuvshintulga, B.; Battsetseg, B.; Battur, B.; Myagmarsuren, P.; Narantsatsral, S.; Sivakumar, T.; Takemae, H.; Igarashi, I.; Inoue, N.; Yokoyama, N.; 五十嵐, 郁男; 井上, 昇; 横山, 直明.
Babesia venatorum, formerly known as Babesia sp. EU1, is a zoonotic hemoprotozoan parasites that commonly infects deer. In the present study, we investigated B. venatorum infection in Ixodes persulcatus, an important tick vector capable of transmitting several tick-borne pathogens that cause babesiosis, encephalitis, tularemia, and Lyme diseases. DNA samples extracted from questing I. persulcatus ticks (n=63) that had been collected in Selenge province of Mongolia in 2012 and 2013 were screened for B. venatorum using a nested PCR assay. The findings showed that two of 63 DNA samples were positive for B. venatorum. The 18S rRNA sequences amplified from B. venatorum-positive DNA samples shared high identity scores (96.1–99.9%) with known B. venatorum...
Palavras-chave: Babesia venatorum; 18S rRNA; Ixodes persulcatus; Mongolia.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4243

The PCR detection and phylogenetic characterization of Babesia microti in questing ticks in Mongolia
Provedor de dados: 19
Autores: Tuvshintulga, Bumduuren; Sivakumar, Thillaiampalam; Battsetseg, Badgar; Narantsatsaral, Sandag-ochir; Enkhtaivan, Batsaikhan; Battur, Banzragch; Hayashida, Kyoko; Okubo, Kazuhiro; Ishizaki, Takahiro; Inoue, Noboru; Igarashi, Ikuo; Yokoyama, Naoaki.
Babesia microti is a tick-transmitted zoonotic hemoprotozoan parasite. In the present study, we investigated B. microti infection in questing ticks in Mongolia. A total of 219 questing ticks were collected from three different Mongolian provinces (Bayan-Olgii, Khovsgol, and Selenge). Of these, 63 from Selenge were identified as Ixodes persulcatus, while the remaining 156 (from all three provinces) were identified as Dermacentor nuttalli. When the tick DNA samples were screened using a B. microti-specific nested PCR, 19 (30.2%) of the 63 I. persulcatus ticks were found to be B. microti-positive. The parasite was not detected in D. nuttalli. Subsequently, the 18S rRNA, cox1, and tufA sequences of B. microti were amplified, sequenced, and subjected to...
Palavras-chave: 18S rRNA; B. microti; Cox1; Mongolia; Ticks; TufA.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4554

Impact of Amazon land use on the community of soil fungi
Provedor de dados: 63
Autores: Fracetto,Giselle G. M.; Azevedo,Lucas C.B.; Fracetto,Felipe J. C.; Andreote,Fernando D.; Lambais,Marcio R.; Pfenning,Ludwig H.
Considered as one of the most biodiverse biomes, the Amazon has a featured role in the discovery of new species of plants, animals and microorganisms, which may be important for the functionality of different ecosystems. However, studies on the impacts resulted from changes in the Amazon land use on microbial communities and their functions are still limited. In this context, the soil fungal diversity can act as an important indicator of environmental stress caused by land use of the Amazon. This study describes changes in soil fungal communities caused by different systems of land use (primary forest, secondary forest, agroforestry, agriculture and pasture). Communities were observed in each of the areas using denaturing gradient gel electrophoresis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DGGE; 18S rRNA; Fungal diversity; Soil biology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000200001