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Comparative study of sulfated polysaccharides from Caulerpa spp. (Chlorophyceae). Biotechnological tool for species identification? - doi: 10.4025/actascibiolsci.v34i4.8976
Provedor de dados: 3
Autores: Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia, Universidade Estadual do Ceará; Quinderé, Ana Luíza Gomes; Universidade Federal do Ceará; Queiroz, Ismael Nilo Lino de; Universidade Federal do Ceará; Coura, Chistiane Oliveira; Universidade Federal do Ceará; Benevides, Norma Maria Barros; Universidade Federal do Ceará.
Studies on macromolecules isolated from marine algae suggested sulfated polysaccharides (SPs) as possible molecular markers for species. We evaluated isolated and fractionated SPs from the green marine algae Caulerpa cupressoides, C. prolifera and C. racemosa collected at Pacheco  Beach, as possible taxonomic molecular indicators. Total SPs were extracted with papain in 100 mM sodium acetate buffer (pH 5.0) containing cysteine and EDTA (both 5 mM), followed by ion-exchange chromatography on DEAE-cellulose using a NaCl gradient. The obtained fractions were analyzed by 0.5% agarose gel electrophoresis. Anticoagulant assays employing normal human plasma and standard heparin (193 IU mg-1) by the activated partial thromboplastin time (APTT) test were also...
Tipo: Bioquímica comparativa de algas
Palavras-chave: 2.08.01.00-9 chlorophyta; Sulfated polysaccharides; Molecular markers; APTT test 2.08.01.00-9.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8976