Consulta: subjectFacets:"454-pyrosequencing"
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Data/hora: 09/06/2024 15:12:09

Characterization of the complete mitochondrial genome and a set of polymorphic microsatellite markers through next-generation sequencing for the brown brocket deer Mazama gouazoubira
Provedor de dados: 74
Autores: Caparroz,Renato; Mantellatto,Aline M.B.; Bertioli,David J.; Figueiredo,Marina G.; Duarte,José Maurício B.
The complete mitochondrial genome of the brown brocket deer Mazama gouazoubira and a set of polymorphic microsatellite markers were identified by 454-pyrosequencing. De novo genome assembly recovered 98% of the mitochondrial genome with a mean coverage of 9-fold. The mitogenome consisted of 16,356 base pairs that included 13 protein-coding genes, two ribosomal subunit genes, 22 transfer RNAs and the control region, as found in other deer. The genetic divergence between the mitogenome described here and a previously published report was ∼0.5%, with the control region and ND5 gene showing the highest intraspecific variation. Seven polymorphic loci were characterized using 15 unrelated individuals; there was moderate genetic variation across most loci (mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Microsatellites; Mitogenome; Population genetics; 454-pyrosequencing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300338