Consulta: subjectFacets:"ARDRA"
Registros recuperados: 10
Data/hora: 13/06/2024 03:45:15

Endophytic bacterial diversity in banana 'Prata Anã' (Musa spp.) roots
Provedor de dados: 74
Autores: Souza,Suzane A.; Xavier,Adelica A.; Costa,Márcia R.; Cardoso,Acleide M.S.; Pereira,Marlon C.T.; Nietsche,Silvia.
The genetic diversity of endophytic bacteria in banana 'Prata Anã' roots was characterized. Two hundred and one endophytic bacteria were isolated, 151 of which were classified as Gram-positive and 50 as Gram-negative. No hypersensitivity response was observed in any of the isolates. The rep-PCR technique generated different molecular profiles for each primer set (REP, ERIC and BOX). Fifty readable loci were obtained and all of the fragments were polymorphic. Amplified ribosomal DNA restriction analysis (ARDRA) of the isolates based on cleavage with four restriction enzymes yielded 45 polymorphic bands and no monomorphic bands. PCR amplified the nifH gene in 24 isolates. 16S rDNA sequencing of the 201 bacterial isolates yielded 102 high-quality sequences....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ARDRA; Bacillus sp.; NifH gene; Rep-PCR; 16S rDNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200016

Produção e comportamento reológico de exopolissacarídeos sintetizados por rizóbios isolados de guandu
Provedor de dados: 86
Autores: Fernandes Júnior,Paulo Ivan; Almeida,João Pedro da Silva; Passos,Samuel Ribeiro; Oliveira,Paulo Jansen de; Rumjanek,Norma Gouvêa; Xavier,Gustavo Ribeiro.
O objetivo deste trabalho foi avaliar a produção e o comportamento reológico de exopolissacarídeos (EPS) produzidos por rizóbios isolados de nódulos de guandu [Cajanus cajan (L.) Millsp.], e a similaridade desses isolados, pela técnica de análise de restrição do DNA ribossomal amplificado. As bactérias foram cultivadas em meio YM líquido e os EPS foram obtidos por precipitação com etanol gelado a partir do caldo de cultivo. Em seguida, eles foram recuperados por centrifugação, secados a vácuo, pesados e ressuspensos em água para avaliações reológicas. Os três isolados avaliados apresentaram diferenças na produção e na eficiência relativa da produção de EPS, com destaque para o isolado 8.1c. Os EPS demonstraram comportamento não newtoniano e pseudoplástico,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cajanus cajan; ARDRA; Biopolímeros; Fixação biológica de nitrogênio; Inovação tecnológica; Reologia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001200018

Ocorrência e diversidade de bactérias diazotróficas associadas a gramíneas forrageiras do Pantanal Sul Matogrossense
Provedor de dados: 90
Autores: Brasil,Marivaine da Silva; Baldani,José Ivo; Baldani,Vera Lucia Divan.
Este trabalho teve por objetivo avaliar a ocorrência e a diversidade genética de bactérias fixadoras de N2 associadas às gramíneas nativas Elyonurus muticus (capim carona) e Axonopus purpusii (capim mimoso) e à gramínea exótica Brachiaria humidicola (braquiária) que formam as pastagens na região da Nhecolândia. As coletas das plantas e solo foram feitas nos períodos de seca e de cheia para determinar a população de bactérias diazotróficas. Identificaram-se Azospirillum brasilense, A. lipoferum, A. amazonense, Herbaspirillum spp., Burkholderia spp. em amostras de solo, raízes e folhas das três espécies forrageiras. As populações dessas bactérias foram menores na época da cheia em comparação com a época da seca. A diversidade genética das bactérias isoladas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fixação biológica de nitrogênio; Azospirillum; ARDRA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832005000200003

Biodiversity of rhizobia associated with cowpea cultivars in soils of the lower half of the São Francisco River Valley
Provedor de dados: 90
Autores: Leite,Jakson; Seido,Sirando Lima; Passos,Samuel Ribeiro; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa; Martins,Lindete Míria Vieira.
The biodiversity of rhizobium in soils of the São Francisco Valley is unknown and can be studied using cowpea as trap plants. The objective of this study was to verify the diversity of diazotrophic bacteria that nodulate cowpea in soils of the lower half of the São Francisco River Valley by morphological and genotypic characterization. Seven soil samples (A1, A2, A3, A4, C1, C2 and MC) were collected to capture bacteria associated to five cowpea cultivars (IPA 206, BRS Pujante, BRS Marataoã, Canapu Roxo, and Sempre Verde), in a 5x7 factorial design with three replications. Thirty days after plant emergence, the nodules were collected and the bacteria isolated and analyzed in relation to their growth characteristics in YMA medium. The 581 isolates were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ARDRA; Biological nitrogen fixation; Vigna unguiculata.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000500015

Ardra profiles of bacteria and archaea in mangrove sediments with different levels of contamination in the estuarine complex of Paranaguá, Brazil
Provedor de dados: 52
Autores: Ribeiro,Catherine Gérikas; Steffens,Maria Berenice Reynaud; Etto,Rafael Mazer; Galvão,Carolina Weigert; Martins,César de Castro; Pedrosa,Fábio de Oliveira; Kolm,Hedda Elisabeth.
The mangrove's sediments from the coastal areas under human activities may contain significant contaminations by hydrocarbons, even when there are no visual evidences of it. The microorganisms are essential to these ecosystems, especially in the control of their chemical environment. Sediment samples were collected in two regions under different environment conditions (pristine and contaminated) of the Paranaguá Estuarine Complex (Paranaguá Bay and Laranjeiras Bay), Brazil. Aliphatic hydrocarbons were determined by the GC-FID to assess the status of contamination of the studied areas. The total DNA was extracted from these samples. The 16S rRNA gene was amplified by the PCR reactions with the pair of primers 21F and 958R for the archaeal domain, and 27F...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diversity; Mangroves; Archaea; Bacteria; 16S rRNA; ARDRA; Aliphatic hydrocarbons.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200013

Characterization of immobilized biomass by amplified rDNA restriction analysis (ARDRA) in an anaerobic sequencing-batch biofilm reactor (ASBBR) for the treatment of industrial wastewater
Provedor de dados: 52
Autores: Sarti,Arnaldo; Pozzi,Eloisa; Zaiat,Marcelo.
The performance of an anaerobic sequencing-batch biofilm reactor (ASBBR- laboratory scale- 14L )containing biomass immobilized on coal was evaluated for the removal of elevated concentrations of sulfate (between 200 and 3,000 mg SO4-2·L-1) from industrial wastewater effluents. The ASBBR was shown to be efficient for removal of organic material (between 90% and 45%) and sulfate (between 95% and 85%). The microbiota adhering to the support medium was analyzed by amplified ribosomal DNA restriction analysis (ARDRA). The ARDRA profiles for the Bacteria and Archaea domains proved to be sensitive for the determination of microbial diversity and were consistent with the physical-chemical monitoring analysis of the reactor. At 3,000 mg SO4-2·L-1, there was a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sulfate removal; Support medium; Endonucleases; ARDRA; Batch reactor; ASBBR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400019

Genetic diversity of siderophore-producing bacteria of tobacco rhizosphere
Provedor de dados: 58
Autores: Tian,Fang; Ding,Yanqin; Zhu,Hui; Yao,Liangtong; Du,Binghai.
The genetic diversity of siderophore-producing bacteria of tobacco rhizosphere was studied by amplified ribosomal DNA restriction analysis (ARDRA), 16S rRNA sequence homology and phylogenetics analysis methods. Studies demonstrated that 85% of the total 354 isolates produced siderophores in iron limited liquid medium. A total of 28 ARDRA patterns were identified among the 299 siderophore-producing bacterial isolates. The 28 ARDRA patterns represented bacteria of 14 different genera belonging to six bacterial divisions, namely ?-, ?-, ?-Proteobacteria, Sphingobacteria, Bacilli,and Actinobacteria. Especially, ?-Proteobacteria consisting of Pseudomonas, Enterobacter, Serratia, Pantoea, Erwinia and Stenotrophomonasgenus encountered 18 different ARDRA groups....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Siderophore; Tobacco rhizosphere; ARDRA; RRNA sequencing.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200013

Genetic diversity of Rhizobia isolates from Amazon soils using cowpea (Vigna unguiculata) as trap plant
Provedor de dados: 58
Autores: Silva,F.V.; Simões-Araújo,J.L.; Silva Júnior,J.P.; Xavier,G.R.; Rumjanek,N.G.
The aim of this work was to characterize rhizobia isolated from the root nodules of cowpea (Vigna unguiculata) plants cultivated in Amazon soils samples by means of ARDRA (Amplified rDNA Restriction Analysis) and sequencing analysis, to know their phylogenetic relationships. The 16S rRNA gene of rhizobia was amplified by PCR (polymerase chain reaction) using universal primers Y1 and Y3. The amplification products were analyzed by the restriction enzymes HinfI, MspI and DdeI and also sequenced with Y1, Y3 and six intermediate primers. The clustering analysis based on ARDRA profiles separated the Amazon isolates in three subgroups, which formed a group apart from the reference isolates of Bradyrhizobium japonicum and Bradyrhizobium elkanii. The clustering...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BNF; Bacteria; 16S rRNA; ARDRA; Sequencing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200033

Characterization of free nitrogen fixing bacteria of the genus Azotobacter in organic vegetable-grown Colombian soils
Provedor de dados: 58
Autores: Jiménez,Diego Javier; Montaña,José Salvador; Martínez,María Mercedes.
With the purpose of isolating and characterizing free nitrogen fixing bacteria (FNFB) of the genus Azotobacter, soil samples were collected randomly from different vegetable organic cultures with neutral pH in different zones of Boyacá-Colombia. Isolations were done in selective free nitrogen Ashby-Sucrose agar obtaining a recovery of 40%. Twenty four isolates were evaluated for colony and cellular morphology, pigment production and metabolic activities. Molecular characterization was carried out using amplified ribosomal DNA restriction analysis (ARDRA). After digestion of 16S rDNA Y1-Y3 PCR products (1487pb) with AluI, HpaII and RsaI endonucleases, a polymorphism of 16% was obtained. Cluster analysis showed three main groups based on DNA fingerprints....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ARDRA; Azotobacter; FNFB; UPGMA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300003

The genetic diversity of genus Bacillus and the related genera revealed by 16S rRNA gene sequences and ardra analyses isolated from geothermal regions of turkey
Provedor de dados: 58
Autores: Cihan,Arzu Coleri; Tekin,Nilgun; Ozcan,Birgul; Cokmus,Cumhur.
Previously isolated 115 endospore-forming bacilli were basically grouped according to their temperature requirements for growth: the thermophiles (74%), the facultative thermophiles (14%) and the mesophiles (12%). These isolates were taken into 16S rRNA gene sequence analyses, and they were clustered among the 7 genera: Anoxybacillus, Aeribacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus, and Thermoactinomycetes. Of these bacilli, only the thirty two isolates belonging to genera Bacillus (16), Brevibacillus (13), Paenibacillus (1) and Thermoactinomycetes (2) were selected and presented in this paper. The comparative sequence analyses revealed that the similarity values were ranged as 91.4-100 %, 91.8- 99.2 %, 92.6- 99.8 % and 90.7 - 99.8 %...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Temperature requirement; Endospore-forming bacilli; 16S rRNA gene; ARDRA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100037