Consulta: subjectFacets:"Activated charcoal"
Registros recuperados: 10
Data/hora: 11/06/2024 12:45:35

In vitro shoot induction and multiplication from nodal segments of adult Ginkgo biloba plants
Provedor de dados: 75
Autores: Mantovani,Nilton César; Grando,Magali F; Xavier,Aloisio; Otoni,Wagner C.
The in vitro performance of herbaceous and woody nodal segments from adult plants and the effect of hydrolyzed casein (HC 500 mg L-1), kinetin (KIN; 6-furfurylaminopurine 0.46 and 4.65 µM) and activated charcoal (AC 1.5 g L-1) were evaluated upon new shoots induction and development, and to establish a system of in vitro propagation from adult plants of Ginkgo biloba. Woody nodal segments did not produce axillary shoots and presented 100% of bacterial and fungal contamination in culture. However, nodal segments from herbaceous shoots were successfully disinfected and displayed high in vitro morphogenic capacity. The HC was essential for the axillary shoots induction and further multiplication, stimulating shoot formation in 85% of the cultured nodal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrolyzed casein; Activated charcoal; Kinetin; Multiple shoots; Herbaceous and woody branches.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200003

Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi:  10.4025/actasciagron.v34i2.12257
Provedor de dados: 1
Autores: Prizão, Eliane Cristina; Universidade Estadual de Maringá; Gonçalves, Letícia de Menezes; Universidade Estadual de Maringá; Gutierre, Maria Auxiliadora; Universidade Estadual de Maringá; Mangolin, Claudete Aparecida; Universidade Estadual de Maringá; Machado, Maria de Fátima Pires da Silva; Universidade Estadual de Maringá.
No previous studies have investigated the use of graphite instead of activated charcoal in orchids. In this work, different concentrations of activated charcoal or graphite were added to KC medium to darken the culture medium and stimulate the in vitro propagation of Cattleya bicolor and of a double hybrid orchid (‘BLC Pastoral Innocence’). The seedlings were inoculated on growth regulator-free KC medium; the effects of activated charcoal (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) and graphite (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) concentrations were tested separately. Each flask contained fifteen seedlings, and the experiments had a randomised, two-way factorial design. The two tested variables were culture medium and illumination levels; eleven culture...
Tipo: Laboratory analysis
Palavras-chave: Activated charcoal; Graphite; Orchids; Micropropagation.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257

Assessment of DNA extraction methods for detection of arbuscular mycorrhizal fungi in plant roots by nested-PCR
Provedor de dados: 3
Autores: Diédhiou, Abdala Gamby; Borges, Wardsson Lustrino; Sadio, Oumar; de Faria, Sergio Miana.
DNA extraction methods were evaluated for the yield and purity of DNA recovered from mycorrhized roots and whether the recovered DNA is suitable for amplification of arbuscular mycorrhizal (AM) fungal SSU rDNA. The DNeasy Plant Mini Kit and three extraction buffers were used alone or in combination with either polyvinylpyrrolidone (PVP), polyvinylpolypyrrolidone (PVPP) and/or activated charcoal (AC). Among the extraction methods tested, those based on the CTAB buffers yielded more DNA than those based on the TE buffer and the DNeasy Plant Mini Kit. Moreover, the use of AC alone or in combination with PVPP reduced DNA yield, while it significantly improved the purity of recovered DNA, whatever the extraction buffer. On the other hand, the success of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Activated charcoal; TE buffer; DNA quality.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/21689

Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
Provedor de dados: 47
Autores: Surenciski,Mauro Rodrigo; Flachsland,Eduardo Alberto; Terada,Graciela; Mroginski,Luis Amado; Rey,Hebe Yolanda.
The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums (0.75 M sucrose (24 h), 0.5 M...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryoconservation; Liquid nitrogen; Calcium alginate; Activated charcoal; Sucrose.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100002

Evaluation of nutrient supplementation to charcoal-treated and untreated rice straw hydrolysate for xylitol production by Candida guilliermondii
Provedor de dados: 52
Autores: Mussatto,Solange Inês; Roberto,Inês Conceição.
Xylitol was produced by Candida guilliermondii from charcoal-treated and untreated rice straw hemicellulosic hydrolysate with or without nutrients (ammonium sulphate, calcium chloride, rice bran extract). Both, xylitol yield and volumetric productivity decreased significantly when the nutrients were added to treated and untreated hydrolysates. In the treated hydrolysate, the efficiency of xylose conversion to xylitol was 79% when the nutrients were omitted. The results demonstrated that rice straw hemicellulosic hydrolysate treated with activated charcoal was a cheap source of xylose and other nutrients for xylitol production by C. guilliermondii. The non-necessity of adding nutrients to the hydrolysate media would be very advantageous since the process...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rice straw hydrolysate; Nutrient; Activated charcoal; Candida guilliermondii; Xylitol.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300020

Micropropagation of Alibertia edulis Rich
Provedor de dados: 52
Autores: Silva,Fernanda Augusta Boato da; Pereira,Luiz Alfredo Rodrigues; Silveira,Conceição Eneida dos Santos.
The aim of this work was to develop a micropropagation protocol for Alibertia edulis, a medicinal Brazilian Cerrado plant. A. edulis seeds were sterilized and germinated in water-agar. Fifteen millimeter long explants from the seedlings cultivated for 50 days were used for the multiplication in three consecutive subcultures. The cytokinin benzyladenine (BA) alone or combined with indole-3-butyric acid (IBA) was added to ¼ MS medium. The treatments containing combinations of the BA + IBA induced more shoot proliferation than the BA alone, with the optimum concentration of 0.5 mg.L-1 for both growth regulators. A. edulis shoots elongated easily and the presence of growth regulators did have a positive effect on the shoot elongation. Consecutive subcultures...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; Alibertia edulis; Rooting; Germination; Activated charcoal.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600004

Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitis
Provedor de dados: 52
Autores: Chaud,Luciana Cristina Silveira; Silva,Débora Danielle Virgínio da; Mattos,Rafael Taino de; Felipe,Maria das Graças de Almeida.
Oat hull hemicellulosic hydrolysate obtained by diluted acid hydrolysis was employed as fermentation medium for Pichia stipitis cultivation. A comparison between the use of treated hydrolysate with 1% activated charcoal to reduce the toxic compounds generated during the hydrolysis process and untreated hydrolysate as a control was conducted. In the cultures using treated hydrolysate the total consumption of glucose, low xylose consumption and ethanol and glycerol formation were observed. The medium formulated with untreated hydrolysate showed morphological cell modifications with consequently cell death, no ethanol formation and formation of glycerol as byproduct of fermentative process, probably as a response to stressful conditions to yeast due to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pichia stipitis; Hemicellulosic hydrolysate; Oat hull; Ethanol; Activated charcoal.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500017

Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification
Provedor de dados: 58
Autores: Dey,Tapati Bhanja; Banerjee,Rintu.
Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by Aspergillus awamori Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (Citrus sinensis var mosambi) peels as solid substrate whereas, α-amylase was produced from A. oryzae (IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charcoal powder in a single step with 65.1%, 69.8% and 60% recoveries, respectively. Apple juice was clarified by these decolourized and partially purified enzymes. In presence of 1% polygalacturonase from mosambi peels (9.87 U/mL) and 0.4% α-amylase (899 U/mL), maximum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polygalacturonase; Α-amylase; Activated charcoal; Decolourization; Apple juice clarification.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100014

Simple and robust DNA extraction method for the large-scale analysis of genotypes containing high polyphenolic content, such as landracesof Solanum tuberosum and Zea mays
Provedor de dados: 118
Autores: Barra,Macarena; Salazar,Erika; Beltrán,María; Sagredo,Boris.
The extraction of high-quality DNA is essential for studies conducted at the molecular level in species with an abundance of contaminants in their tissues, such as some landraces of potato and maize, in which it is difficult to extract good-quality genomic DNA. Compounds such as polyphenols interfere with the amplification of DNA during polymerase chain reaction (PCR), which makes it difficult to conduct PCR-based studies ofmolecular markers. This article describes a simple and robust protocol for DNA isolation that was applied to potato and maize landraces and resulted in a high DNA concentration with excellent purity and quality. This method is based on the method of Krizman et al. (2006) with some modifications and was tested on potato leaves and young...
Tipo: Journal article
Palavras-chave: Activated charcoal; DNA extraction; Genomic DNA; Polyphenols.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300019

In vitro behaviour of Aspasia variegata, an epiphytic orchid from the Brazilian Cerrado
Provedor de dados: 65
Autores: Paiva Neto,Vespasiano Borges de; Campos,Guilherme de Oliveira; Boaretto,Amanda Galdi; Zuffo,Monica Cristina Rezende; Torrezan,Mateus de Aguiar; Benetão,Jamile.
Aspasia variegata occurs naturally in the savanna of the Mato Grosso do Sul State, Brazil and it has been widely collected for its beautiful flowers. Additionally, its habitat has been greatly reduced and little or no investigation of its spread has been performed. Aiming to establish a protocol to obtain seedlings of the orchid A. variegata, different compositions of culture medium were tested to identify which one provided better in vitro growth and development and to assess the influence of these media in seedling acclimatisation. Thus, seeds obtained from mature capsules were inoculated in Knudson culture medium for 120 days until the protocorm stage. They were transferred to different culture media formulations, including MS and Knudson with half or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orchidaceae; Activated charcoal; Chlorophyll content; Acclimatisation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001200010