Consulta: subjectFacets:"Adhesion"
Registros recuperados: 53
Data/hora: 01/06/2024 12:16:14

Evaluating kinetic and physiological features of rCHO-K1 cells cultured on microcarriers for production of a recombinant metalloprotease/disintegrin
Provedor de dados: 69
Autores: Swiech,Kamilla; da Silva,Gracinda M.C; Zangirolami,Teresa C; Iemma,Mônica R.C; Selistre-de-Araújo,Heloísa S; Suazo,Cláudio A.T.
We present kinetic and physiological data regarding the culturing of rCHO-K1 cells on various microcarriers, to evaluate the potential of this culture strategy for mass production of these cells and expression of a recombinant disintegrin. Cultures were performed in 500 mL spinner flasks in DMEM culture medium with 10% v/v fetal calf serum, gently shaken at 37ºC, pH 7.4, in a 10% v/v CO2 atmosphere. The following values were obtained, respectively, for the adhesion time-constant Ka (h) and specific growth rate &amp;#956;max (d-1) on each microcarrier: Cytodex 1 (0.91, 0.45), Cultispher S (0.28, 0.34), Immobasil FS (0.85, 0.52) and Pronectin F (5.12, 0.67). Metabolic characteristics showed some variation among the cultures with the four microcarriers, the...
Tipo: Journal article
Palavras-chave: Adhesion; Animal cell; CHO-K1; Growth; Microcarrier; Recombinant disintegrin.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200004

Effects of extracellular products from the pathogenic Vibrio aestuarianus strain 01/32 on lethality and cellular immune responses of the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Labreuche, Yannick; Soudant, Philippe; Goncalves, Madeleine; Lambert, Christophe; Nicolas, Jean-louis.
Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced...
Tipo: Text
Palavras-chave: Vibrio aestuarianus; ECPs; Crassostrea gigas; ROS production; Adhesion; Phagocytosis; Flow cytometry; Bivalve immunity.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1035.pdf

Lutte contre les salissures marines : approche par procédés enzymatiques.
Provedor de dados: 5
Autores: Leroy, Celine.
Fouling on marine underwater surfaces causes critical and economic problems such as important material biodamages and industrial performances reduction. We chose to test antifouling potential of enzymatic commercial preparations like hydrolases (proteases, glycosidases and lipases) in order to inhibit the first fouling adhesion step: bacterial biofilm formation. An evaluation test of antifouling properties onto marine bacterial adhesion was designed using a mono-incubation of Pseudoalteromonas sp. D41 in microtiter plate and in sterile natural sea water. This test was adapted to screen agents for bacterial adhesion removal or inhibition activities and allowed to test enzymatic preparations toxicity on non adhered bacteria. Inhibition rates according to...
Tipo: Text
Palavras-chave: Fouling; Adhesion; Antifouling; Hydrolases; EPS; DAPI; Microtiter plate; Screening; Pseudoalteromonas sp.; Marine biofilm; Salissure marine; Adhésion; Antisalissure; Hydrolases; EPS; DAPI; Microplaque; Criblage; Pseudoalteromonas sp.; Biofilm marin.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/these-1099.pdf

Development of an improved adhesive test method for composite assembly design
Provedor de dados: 5
Autores: Cognard, Jean Yves; Davies, Peter; Gineste, B; Sohier, L.
This study is concerned with improving performance of composite structures through the use of adhesive bonding, particularly for marine and underwater applications. Some preliminary results from tests on simple composite/composite assemblies are given first. Difficulty in modelling the failure of even these simple joints highlighted the need for more reliable constituent input data. The first objective was to define an experimental methodology enabling the adhesives of interest to be characterised up to failure. A metal metal assembly was considered, in order to concentrate on the analysis of the behaviour of thin adhesive films. The aims were to characterise the adhesive, analyse its non-linear behaviour, and study the influence of parameters such as film...
Tipo: Text
Palavras-chave: Finite element analysis; Adhesion.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-765.pdf

Hemocyte characteristics in families of oysters, Crassostrea gigas, selected for differential survival during summer and reared in three sites
Provedor de dados: 5
Autores: Lambert, Christophe; Soudant, Phillippe; Degremont, Lionel; Delaporte, Maryse; Moal, Jeanne; Boudry, Pierre; Jean, Frédéric; Huvet, Arnaud; Samain, Jean-francois.
High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Riviere d'Auray (Brittany) and Ronce (Marennes-oleron Basin, Poitou Charentes).   Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered...
Tipo: Text
Palavras-chave: Genetic; Flow cytometry; Reactive oxygen species ROS; Adhesion; Hemocyte; Crassostrea gigas; Summer mortality.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3054.pdf

Development of alginate hydrogels active against adhesion of microalgae
Provedor de dados: 5
Autores: Nassif, L. Abi; Rioual, S.; Trepos, Rozenn; Fauchon, M.; Farah, W.; Hellio, C.; Abboud, M.; Lescop, B.
Microorganisms have the ability to settle on nearly all man-made surfaces in contact with seawater and subsequently to form biofilm. Biofilms control and removal is necessary in the sectors of maritime transport, energy... In this work, we present the development of new pure calcium, zinc or copper alginate, but also mixed Ca/Cu and Ca/Zn alginate hydrogels. These materials have been evaluated for their potential inhibition of adhesion of two key biofilm-forming microalgae Halamphora coffeaeformis and Cylindrotheca closterium). All the tested materials have presented high adhesion inhibition about 80%). Copper-base materials present a high toxicity against H. coffeaeformis. Pure zinc alginate is also toxic for this strain. However, the addition of calcium...
Tipo: Text
Palavras-chave: Biofilm; Adhesion; Alginate; Zinc; Biomaterials; Microalgae.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00605/71671/70168.pdf

Testing the effectiveness of tough resin systems for improving structural performance of joints in GRP ships.
Provedor de dados: 5
Autores: Norwood, L; Caulier, C.
A major concern in any composite structure is its ability to resist peel and shear loading; this becomes a more difficult problem where long delays in laminating have occurred or when dissimilar materials are joined together. In the case of mine countermeasure vessels, adhesion between stiffener and hull becomes critical when the ship is subjected to underwater shock loading. In order to reduce costs and improve the hull-stiffener bond, tough resin systems based on urethane acrylates have been developed to replace the mechanical crack-stopping system currently employed. This paper describes the benefits of urethane acrylates in the frame-hull application discussing the level of improvement over the mechanical system.
Tipo: Text
Palavras-chave: Ships; Adhesion; Joints; Shear; Ship technology; Glass reinforced plastics; Materials testing; Composite materials; Urethane acrylate; Raidisseur; Coque; Fixation; Joint.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1043.pdf

Etude au microscope electronique a balayage des relations entre bacteries et matieres en suspension en milieu marin
Provedor de dados: 5
Autores: Cormier, M; Bassoulet, P; Pommepuy, Monique.
Near outfall areas numerous bacteria appear associated to colloidal organic matter present in littoral sea water. Arrangements into colonies are shown. Surface structures are observed which allow bacteria's attachment each to other and to organic matter.
Tipo: Text
Palavras-chave: Bacteria; Outfalls; Electron microscopy; Flocculation; Colloids; Adhesion; Suspended particulate matter.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-966.pdf

Effects of commercial enzymes on the adhesion of a marine biofilm-forming bacterium
Provedor de dados: 5
Autores: Leroy, C.; Delbarre Ladrat, Christine; Ghillebaert, F.; Compere, Chantal; Combes, D.
The antifouling potential of commercial hydrolases, four proteases, seven glycosidases and one lipase was evaluated on the adhesion of marine Pseudoalteromonas sp. D41. The experimental method, adapted to screen antifouling agents, was based on bacterial adhesion in natural sterile sea water in a microtiter plate and on total biomass quantification by the fluorescent dye DAPI (4[prime]6-diamidino-2-phenylindole). Savinase (subtilisin) was the most effective hydrolase in both the prevention of bacterial adhesion and the removal of adhered bacteria. However, some enzymatic preparations tested such as Amano protease were not only ineffective but also increased the number of adhered bacterial cells. Enumeration using epifluorescence microscopy of CTC...
Tipo: Text
Palavras-chave: Marine biofilm; Antifouling; Pseudoalteromonas; Enzymes; Adhesion.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-6140.pdf

Recherche de defauts de collage dans les structures sandwich
Provedor de dados: 5
Autores: Darold, L; Blanco, E.
One of the major defects that can be found on composite sandwich structures is poor bonding of the core on the exterior skins, particularly with the technique of bonding under vacuum. Therefore, we have looked at all the possible means of detecting these bonding defects, with two objectives: to be able to control integrally a piece of large dimensions, and to be able to control the piece in the course of manufacturing, that is to say after the bonding on the first skin. All of the non-destructive test methods now known have therefore been tested, and in particularly infrared thermography, ultrasonic control, and acoustic methods. This study, carried out in collaboration with the CETIM, the STCAN, Aerospatiale and other laboratories and companies without...
Tipo: Text
Palavras-chave: Adhesion; Ship technology; Composite materials; Contrôle non destructif; Structure sandwich.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1049.pdf

A study of the non-linear behaviour of adhesively-bonded composite assemblies
Provedor de dados: 5
Autores: Cognard, Jean Yves; Davies, Peter; Sohier, S; Creac' Hcadec, R.
The objective of this study is to define a reliable tool for dimensioning of adhesively bonded assemblies, particularly for marine and underwater applications. This paper presents experimental and numerical results, which describe the non-linear behaviour of an adhesive in a bonded assembly for various loadings. A modified Arcan fixture, well-suited for the study of the behaviour of bonded metal-metal assemblies, was developed in order to focus on the analysis of the behaviour of the adhesive in thin films. The edge effects are minimised by using an appropriate design of the substrates so that experimental results give reliable data. To analyze the kinematics of the deformation of the adhesive joint non-contact extensometry techniques were employed. Thus,...
Tipo: Text
Palavras-chave: Non linear behaviour; Finite element; Arcan fixture; Marine structures; Composite; Adhesion.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2079.pdf

Exploring Antifouling Activity of Biosurfactants Producing Marine Bacteria Isolated from Gulf of California
Provedor de dados: 5
Autores: Alemán-vega, Monserrat; Sánchez-lozano, Ilse; Hernández-guerrero, Claudia J.; Hellio, Claire; Quintana, Erika T.
Biofouling causes major problems and economic losses to marine and shipping industries. In the search for new antifouling agents, marine bacteria with biosurfactants production capability can be an excellent option, due to the amphipathic surface-active characteristic that confers antimicrobial and antibiofilm activities. The aim of this study was to evaluate the antifouling activity of biosurfactants producing marine bacteria from the Gulf of California. The cell free culture supernatant (CFCS) of Bacillus niabensis (S-69), Ralstonia sp. (S-74) (isolated from marine sediment) and of B. niabensis (My-30) (bacteria associated to the sponge Mycale ramulosa) were screened for production of biosurfactants (using hemolysis and drop collapse test, oil...
Tipo: Text
Palavras-chave: Adhesion; Biofilm; Biosurfactants; Environmentally friendly antifouling; Field assays; Bacillus niabensis; Ralstoniasp.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00646/75804/76733.pdf

Applications marines des composites a matrice metallique d'aluminium assembles par collage (MMC)
Provedor de dados: 5
Autores: Davies, R; Ritchie, M; Trumper, R.
Metal matrix composites (MMC) offer a unique combination of properties that make this class of material ideally suited to the fabrication of components subjected to complex loads, such as marine structures. In many marine applications the dominant requirement is for a reduction in structural weight, and where failure occurs by elastic buckling stiffness is critical. MMC's encompass a very broad class of materials which may be subdivided into three main groups: particulate, whisker and continuous fibre reinforced metals. These materials offer improvements in stiffness and the strength to weight ratio over conventional alloys. However, the ability to construct efficient marine structures is constrained by the ability to join these materials both to...
Tipo: Text
Palavras-chave: Adhesion; Adhesives; Ship technology; Composite materials; Traitement de surface; Collage; Structures marines; Composites à matrice métallique.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1079.pdf

A marine bacterial adhesion microplate test using the DAPI fluorescent dye: a new method to screen antifouling agents
Provedor de dados: 5
Autores: Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Rochet, Marie-joelle; Compere, Chantal; Combes, D.
Aims: To develop a method to screen antifouling agents against marine bacterial adhesion as a sensitive, rapid, quantitative microplate fluorescent test.  Methods and Results: Our experimental method is based on a natural biofilm formed by mono-incubation of the marine bacterium Pseudoalteromonas sp. D41 in sterile natural sea water in a 96 wells polystyrene microplate. The 4'6-diamidino-2-phenylindole (DAPI) dye was used to quantify adhered bacteria in each well. Total measured fluorescence in wells was correlated with the amount of bacteria showing a detection limit of one bacterium per 5 µm2 and quantifying 2 x 107 to 2 x 108 bacteria adhered per cm2. The antifouling properties of three commercial surface active agents and chlorine were tested by this...
Tipo: Text
Palavras-chave: Antifouling; Pseudoalteromonas; Screening; Microtiter plate; Adhesion; DAPI; Marine biofilm.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2006/publication-2017.pdf

Effects of environmental conditions on the sessile existence of an estuarine sediment bacterium.
Provedor de dados: 5
Autores: Geesey, G; Salas, S; Mittelman, M.
A submerged glass coverslip technique was developed to determine dissolved organic nutrient uptake by adherent cells of a sediment isolate of Enterobacter cloacae . Cells which colonized the coverslips in swirling culture flasks during anaerobic growth remained firmly attached during manipulations employed to determine uptake of radiolabeled glucose by the adherent population. The attached cells were capable of a more rapid rate of glucose uptake than free cells. Lineweaver-Burk plots demonstrated different glucose uptake kinetics for the 2 cell populations. The data suggest that physiological changes occur in cells soon after they become attached to surfaces. These changes appear to enhance the metabolic activity of the adherent population.
Tipo: Text
Palavras-chave: Enterobacter cloacae; Adhesion; Glucose; Environmental effects; Bacteria; Sediments; Estuaries; Sessile species; Culture media; Metabolism; Dissolved oxygen; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-967.pdf

Etude experimentale de la colonisation de surfaces par les bacteries, en eau de mer circulante
Provedor de dados: 5
Autores: Fera, P; Prieur, D.
Before the building of an (OTEC) power plant, and experimental station was built near the entrance of the Bay of Brest in order to do preliminary studies. Using these facilities, the bacterial colonization of three substrata (stainless steel UB6, aluminium AG4, polycarbonate filters) was studied in an open seawater system, with a speed of 0,7 m s super(-1). The study was performed between May 1983 and May 1984, including seven experiments of 15 days each. Bacteria colonizing the metallic surfaces were counted by scanning electron microscopy, and bacteria colonizing the polycarbonate filters counted by epifluorescence microscopy. On the three substrata, bacterial colonization occurred in three successive stages: initial colonization, sometimes unsteady...
Tipo: Text
Palavras-chave: Fouling; OTEC plants; Bacteria; Colonization; Adhesion; Microbiological analysis; Surface films.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-964.pdf

A modified Arcan test to analyze the behavior of composites and their assemblies under out-of-plane loadings
Provedor de dados: 5
Autores: Cognard, J. Y.; Sohier, L.; Davies, Peter.
Composite materials are a key element in weight reduction strategies, so the analysis of the mechanical behavior of assemblies of composite modules is of great importance. Failure initiation in bonded assemblies involving composites is often associated with crack initiation in the adhesive or delamination of the composite plies close to the adhesive joint, caused by interlaminar or through-thickness stresses. Thus, the analysis of the behavior of composites and their assemblies under out-of-plane loadings is necessary in order to optimize such structures. However, few experimental devices are proposed in the literature and they often require thick composite specimens which are not representative of most industrial applications. This paper describes the use...
Tipo: Text
Palavras-chave: Laminates; Adhesion; Stress concentrations; Mechanical testing.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00026/13740/11039.pdf

Biomimetic Approaches for the Development of New Antifouling Solutions: Study of Incorporation of Macroalgae and Sponge Extracts for the Development of New Environmentally-Friendly Coatings
Provedor de dados: 5
Autores: Sánchez-lozano, Ilse; Hernández-guerrero, Claudia Judith; Muñoz-ochoa, Mauricio; Hellio, Claire.
Biofouling causes major economic losses in the maritime industry. In our site study, the Bay of La Paz (Gulf of California), biofouling on immersed structures is a major problem and is treated mostly with copper-based antifouling paints. Due to the known environmental effect of such treatments, the search for environmentally friendly alternatives in this zone of high biodiversity is a priority to ensure the conservation and protection of species. The aim of this work was to link chemical ecology to marine biotechnology: indeed, the natural defense of macroalgae and sponge was evaluated against biofoulers (biofilm and macrofoulers) from the same geographical zone, and some coatings formulation was done for field assays. Our approach combines in vitro and...
Tipo: Text
Palavras-chave: Adhesion; Algae; Antifouling; Biofilm; Extracts; Field assays; Sponges; Haliclona caerulea; Laurencia gardneri; Sargassum horridum; Ircinia sp.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00589/70074/68047.pdf

Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells
Provedor de dados: 77
Autores: Chiapparrone,María L; Soto,Pedro; Catena,María.
Campylobacter fetus is extracellular bacteria of the genital tract of cattle. They cause infertility and abortion, but there is no documented information on the susceptibility of bovine sperm cells to this bacteria. The aim of this present work was to study the effects provoked by Campylobacter fetus subsp. venerealis when in interaction with bovine sperm cells. The bovine spermatozoa were obtained frozen bovine semen pooled from uninfected bulls, and were exposed to living campylobacter over different periods of time. Light microscopy and scanning electron microscopy first revealed a tropism, then a close proximity followed by tight adhesion between these two different cells. A decrease in the spermatozoa motility was observed. Motile bacteria were...
Tipo: Journal article
Palavras-chave: Adhesion; Campylobacter fetus subsp. venerealis; Sperm cells.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400040

In-vitro Comparative Study of the use of 2 % Chlorhexidine on Microtensile Bond Strength of Different Dentin Adhesives: A 6 Months Evaluation
Provedor de dados: 77
Autores: Bravo,Cristián; Sampaio,Camila S; Hirata,Ronaldo; Puppin-Rontani,Regina M; Mayoral,Juan Ricardo; Giner,Lluis.
The aim of this study was to evaluate the effect of chlorhexidine (CHX) dentin treatment on microtensile bond strength (µTBS) of adhesive systems in different storage times. Occlusal enamel was removed from ninety third-molars and flat surfaces of middle dentin were exposed. Teeth were randomly divided in 6 groups according to adhesive system (etch-and-rinse : Adper Scotchbond 1XT - ASB ; self-etch: Adper Prompt L-Pop ­ APP; and universal: Single Bond Universal - SBU) and chlorhexidine (CHX) dentin treatment (2 % CHX application for 20 s prior Primer). After resin composite build up, teeth were sectioned to obtain beam specimens and divided in 3 subgroups (n=5): 72h, 3 and 6 months storage times. After the storage times, teeth were tested in tension until...
Tipo: Journal article
Palavras-chave: Microtensile bond strength; Dentin; Chlorhexidine; Dentin adhesives; Adhesion.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300016

Effect of 2 % Chlorhexidine on Dentin Shear Bond Strength of Different Adhesive Systems: A 6 Months Evaluation
Provedor de dados: 77
Autores: Bravo,Cristián; Sampaio,Camila S; Hirata,Ronaldo; Puppin-Rontani,Regina M; Mayoral,Juan Ricardo; Giner,Lluis.
This study evaluated the effect of chlorhexidine dentin treatment on shear bond strength (SBS) of adhesive systems after different storages. The work included 144 third molars that had their dentin exposed and were divided in 6 groups: G1 (ASB+CHX: Adper Scotchbond 1XT + chlorhexidine 2 % prior Primer); G2(ASB); G3 (APP+CHX: Adper Prompt L-Pop + CHX); G4(APP); G5 (SBU+CHX: Single Bond Universal + CHX); and G6(SBU). Resin build-up was performed and teeth were subdivided regarding storage times (n=8): 72 h, 3 and 6 months. Next, SBS test was performed. At 72 hours, all equivalent groups (same adhesive system, different dentin treatment) showed no significant difference in SBS (P.05). Self-etch adhesive groups (with or without CHX) presented lower SBS...
Tipo: Journal article
Palavras-chave: Shear bond strength; Dentin bond strength; Chlorhexidine; Adhesive systems; Adhesion.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300052

Gross and Histologic Evaluation of Abdominal Adhesions Associated with Chromic Catgut and Polypropylene Sutured Enteropexies in Dog
Provedor de dados: 77
Autores: Akinrinmade,J. F; Lawal,A. O.
The gross and histologic patterns of abdominal adhesions formed by the use of chromic catgut and polypropylene suture materials were evaluated in dogs. Gross and histologic examinations did not reveal significant difference (p&gt;0.05) in the degree of adhesions observed at enteropexy sites in both suture materials. The use of polypropylene with superior suture qualities should be considered instead of chromic catgut for procedures in which adhesion is desired in the dog.
Tipo: Journal article
Palavras-chave: Adhesion; Chromic catgut; Polypropylene; Eneropexy.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400037

In-Vitro Adhesion and Invasion Properties of Salmonella Typhimurium Competing with Bacteriophage in Epithelial Cells and Chicken Macrophages
Provedor de dados: 89
Autores: Lee,HY; Biswas,D; Ahn,J.
ABSTRACT This study was designed to assess the role of bacteriophage P22 in the adhesion, invasion, intracellular survival of, and cellular immune response to Salmonella Typhimurium in intestinal epithelial INT-407 and chicken macrophage-like HD11 cells. The ability of S. Typhimurium to adhere, invade, and survive to INT-407 and HD11cells was evaluated under Salmonella infection alone (control), phage treatment followed by Salmonella infection (PS), Salmonella infection followed by phage treatment (SP), and a combination treatment with Salmonella and phage (S+P). The number of S. Typhimurium associated on INT-407 cells was reduced from 4.2 to 2.7 log cfu/cm2 by phage treatment (SP). The number of intracellular S. Typhimurium within INT-407 cells was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Bacteriophage; Invasion; Salmonella; Toll-like receptor.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400427

Handling sticky resin by stingless bees (Hymenoptera, Apidae)
Provedor de dados: 92
Autores: Gastauer,Markus; Campos,Lucio A. O.; Wittmann,Dieter.
For their nest defense, stingless bees (Meliponini) collect plant resins which they stick on intruders like ants or cleptobiotic robber bees causing their immobilization. The aim of this article is to identify all parts of stingless bee workers contacting these sticky resins. Of special interest are those body parts with anti-adhesive properties to resin, where it can be removed without residues. For that, extensive behavioral observations during foraging flight, handling and application of the resin have been carried out. When handling the resin, all tarsi touch the resin while walking above it. For transportation from plants to the nest during foraging flight, the resin is packed to the corbicula via tarsi and basitarsi of front and middle legs. Once...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Meliponini; Nest defense; Surface properties.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262011000200014

Flocculation of fine fluorite particles with Corynebacterium xerosis
Provedor de dados: 59
Autores: Haas,Sandra R.; Nascimento,Fábio R.; Schneider,Ivo André H.; Gaylarde,Christine.
The treatment of fine particles dispersed in liquids is common in several industries and especially important in mineral processing. The efficiency of settling operations can be substantially increased by flocculation. The aim of this work was to study the flocculation of fine fluorite particles by the bacterium Corynebacterium xerosis. Flocculation tests, microelectrophoresis measurements and optical microscopy were used to evaluate flocculation. The results showed that C. xerosis cells adhere to the fluorite surfaces promoting the aggregation of the particles. High quality flocs can be obtained rapidly at pH 7.0 using a cell concentration of 40 mg/l, considerably lower than previously reported in the literature. The results are discussed with reference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Corynebacterium xerosis; Flocculation; Fluorite.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300007

The ubiquitous gp63-like metalloprotease from lower trypanosomatids: in the search for a function
Provedor de dados: 42
Autores: Santos,André L.S.; Branquinha,Marta H.; D'Avila-Levy,Claudia M.
Plant and insect trypanosomatids constitute the "lower trypanosomatids", which have been used routinely as laboratory models for biochemical and molecular studies because they are easily cultured under axenic conditions, and they contain homologues of virulence factors from the classic human trypanosomatid pathogens. Among the molecular factors that contribute to Leishmania spp. virulence and pathogenesis, the major surface protease, alternatively called MSP, PSP, leishmanolysin, EC 3.4.24.36 and gp63, is the most abundant surface protein of Leishmania promastigotes. A myriad of functions have been described for the gp63 from Leishmania spp. when the metacyclic promastigote is inside the mammalian host. However, less is known about the functions performed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosomatidae; Lower trypanosomatids; Gp63; Invertebrate host; Adhesion; Nutrition; Protease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400006

The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1
Provedor de dados: 42
Autores: DRAKULIC,DANIJELA; VICENTIC,JELENA MARJANOVIC; SCHWIRTLICH,MARIJA; TOSIC,JELENA; KRSTIC,ALEKSANDAR; KLAJN,ANDRIJANA; STEVANOVIC,MILENA.
The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; HDM2; Migration; P53; SOX2; Wound healing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100389

Association of number of tandem repeats in two important adhesins in Mycoplasma hyopneumoniae
Provedor de dados: 45
Autores: Santos,L. F. dos; Alvarez,J.; Perez,A.; Moreira,M. A. S.; Pieters,M.
RESUMODiversidade genética de Mycoplasma hyopneumoniae tem sido relatada em análise múltipla de repetições em tandem em número variável (MLVA). O objetivo deste estudo foi descrever a distribuição espacial e a heterogeneidade genética de tipos de M. hyopneumoniae no Brasil, bem como investigar a correlação entre regiões de repetição 1 (RR1) e 3 (RR3) de duas adesinas importantes (P97 e P146). Foram identificados 39 tipos de MLVA baseados no número de repetições em tandem em P97 RR1 e RR3 P146. A correlação negativa significativa (Spearman's rho = -0,26; P = 0,022) entre P97 RR1 e RR3 P146 foi observada, o que sugere um possível mecanismo compensatório que permitiria a bactéria manter a sua capacidade de adesão. Os resultados contribuem para compreender a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Adhesion; VNTR; MLVA; Swine; Enzootic pneumonia.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000501461

Regulation of Rho Family GTPases by Cell-Cell and Cell-Matrix Adhesion
Provedor de dados: 48
Autores: ARTHUR,WILLIAM T.; NOREN,NICOLE K.; BURRIDGE,KEITH.
Integrins and cadherins are transmembrane adhesion receptors that are necessary for cells to interact with the extracellular matrix or adjacent cells, respectively. Integrins and cadherins initiate signaling pathways that modulate the activity of Rho family GTPases. The Rho proteins Cdc42, Rac1, and RhoA regulate the actin cytoskeleton. Cdc42 and Rac1 are primarily involved in the formation of protrusive structures, while RhoA generates myosin-based contractility. Here we examine the differential regulation of RhoA, Cdc42, and Rac1 by integrin and cadherin signaling. Integrin and cadherin signaling leads to a decrease in RhoA activity and activation of Cdc42 and Rac1. When the normal RhoA suppression is antagonized or RhoA signaling is increased, cells...
Tipo: Journal article
Palavras-chave: Integrins; Cadherins; Adhesion; Rho; Rac; Cdc42.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200016

The expression of heterologous MAM-7 in Lactobacillus rhamnosus reduces its intrinsic capacity to inhibit colonization of pathogen Vibrio parahaemolyticus in vitro
Provedor de dados: 48
Autores: Beltran,Sebastian; Munoz-Bergmann,Cristian A; Elola-Lopez,Ana; Quintana,Javiera; Segovia,Cristopher; Trombert,Annette N.
BACKGROUND: Vibrio parahaemolyticus (V. parahaemolyticus) is a Gram-negative, halophilic bacterium recognized as one of the most important foodborne pathogen. When ingested, V. parahaemolyticus causes a self-limiting illness (Vibriosis), characterized mainly by watery diarrhoea. Treatment is usually oral rehydration and/or antibiotics in complicated cases. Since 1996, the pathogenic and pandemic V. parahaemolyticus O3:K6 serotype has spread worldwide, increasing the reported number of vibriosis cases. Thus, the design of new strategies for pathogen control and illness prevention is necessary. Lactobacillus sp. grouped Gram positive innocuous bacteria, part of normal intestinal microbiota and usually used as oral vaccines for several diarrheic diseases....
Tipo: Journal article
Palavras-chave: Lactobacillus; V. parahaemolyticus; Adhesion; Recombinants probiotics.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100002

In vitro evaluation of adhesion and aggregation abilities of four potential probiotic strains isolated from guppy (Poecilia reticulata)
Provedor de dados: 52
Autores: Balakrishna,Aparna.
The aim of this work was to study the probiotic-related characteristics of four strains of bacteria isolated from the normal flora of the guppy, Poecilia reticulata. In vitro results showed that the strains, namely, MBTU_PB1, MBTU_PB2, MBTU_PB3 and MBTU_PB4 had higher adhesion abilities than the tested indicator strains. However, an association between the cell-surface hydrophobicity and the ability to adhere to the intestinal mucus was not observed for these strains. Further, the selected strains were strongly autoaggregating (autoaggregation percentage ≥ 80) and also showed strain-specific coaggregation abilities with the tested indicator strains.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probiotics; Adhesion; Aggreggation; Hydrophobicity; Poecilia reticulata.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500010

Xylitol inhibits J774A.1 macrophage adhesion in vitro
Provedor de dados: 52
Autores: Ferreira,Aline Siqueira; Souza,Maria Aparecida de; Raposo,Nádia Rezende Barbosa; Ferreira,Ana Paula; Silva,Silvio Silvério da.
The aim of this work was to evaluate the effect of xylitol on J774A.1 macrophage adhesion. Adhesion consisted of a three-hour interval, at room temperature, followed by washing and cell incubation at 37ºC/5% CO2/ 48h. Xylitol was used to treat the cells either before (for 24h) or after the cell incubation (for 48h) at 5% as final concentration in both the situations. It was found that xylitol was effective in preventing the adhesion in both the conditions in spite of the former being 100-fold greater and significant (p &lt; 0.001). The results pointed to an important xylitol action on macrophage adhesion, which should be further investigated as an inflammatory control.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; J774A; 1 macrophages; Xylitol.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600017

Influence of gastrointestinal system conditions on adhesion of exopolysaccharide-producing Lactobacillus delbrueckii subsp. bulgaricus strains to caco-2 cells
Provedor de dados: 52
Autores: Darilmaz,Derya Onal; Aslım,Belma; Suludere,Zekiye; Akca,Gülcin.
This study aimed to assess the transit tolerance of potential probiotic dairy Lactobacillus strains in human uppergastrointestinal tract in vitro, and to evaluate the effect of EPS production on the viability and adhesion of these strains. Survival and adhesion of two exopolysaccharide (EPS)-producing L. delbrueckii subsp. bulgaricus strains (B3 and B2) and E. coli ATCC11229 were assessed after the exposure of different pH (gastric juice) and gastric plus pancreatic juice challenges. In the artificial gastric juice (pH 2), both the viability of the strain B3 and B2 was decreased. Artificial juice treatments significantly reduced the adhesion to caco-2 cells (P&lt; 0.05). High EPS-producing B3 survived better in the adverse gastrointestinal conditions and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Exopolysaccharide; Caco-2 Cells; Gastric-pancreatic juices; Lactobacillus delbrueckii subsp. bulgaricus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500009

Notch ligand Delta-like 1 promotes the metastasis of melanoma by enhancing tumor adhesion
Provedor de dados: 56
Autores: Zhang,J.P.; Li,N.; Bai,W.Z.; Qiu,X.C.; Ma,B.A.; Zhou,Y.; Fan,Q.Y.; Shan,L.Q.
Notch signaling plays a vital role in tumorigenicity and tumor progression by regulating proliferation, invasion, and the tumor microenvironment. Previous research by our group indicated that Notch ligand Delta-like 1 (Dll1) is involved in angiogenesis in melanoma, and we noticed that it took a longer time to trypsinize Dll1-expressing B16 melanoma cells than the control cells. In this article, we extended our study to investigate the effects of Dll1 on tumor cell adhesion and metastasis. Dll1 overexpression activated Notch signaling in B16 tumor cells and significantly enhanced the adhering capacity of B16 tumor cells both in vitro and in vivo. B16-Dll1 cells also had a higher metastatic potential than their counterpart in the mouse model of lung...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Delta-like 1; Notch signaling; Melanoma; Metastasis; Adhesion; N-cadherin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400299

Galectins: a key intersection between glycobiology and immunology
Provedor de dados: 56
Autores: Rabinovich,G.A.; Riera,C.M.; Landa,C.A.; Sotomayor,C.E.
Galectins are a family of evolutionarily conserved animal lectins, widely distributed from lower invertebrates to mammals. They share sequence and structure similarities in the carbohydrate recognition domain and specificity for polylactosamine-enriched glycoconjugates. In the last few years significant experimental data have been accumulated concerning their participation in different biological processes requiring carbohydrate recognition such as cell adhesion, cell growth regulation, inflammation, immunomodulation, apoptosis and metastasis. In the present review we will discuss some exciting questions and advances in galectin research, highlighting the significance of these proteins in immunological processes and their implications in biomedical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galectins; Immunomodulation; Apoptosis; Adhesion; Embryogenesis; Metastasis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400002

Human sepsis-associated Escherichia coli (SEPEC) is able to adhere to and invade kidney epithelial cells in culture
Provedor de dados: 56
Autores: Conceição,R.A.; Ludovico,M.S.; Andrade,C.G.T.J.; Yano,T.
The adhesins of extraintestinal pathogenic Escherichia coli are essential for mediating direct interactions between the microbes and the host cell surfaces that they infect. Using fluorescence microscopy and gentamycin protection assays, we observed that 49 sepsis-associated E. coli (SEPEC) strains isolated from human adults adhered to and invaded Vero cells in the presence of D-mannose (100%). In addition, bacteria concentrations of approximately 2 x 10(7) CFU/mL were recovered from Vero cells following an invasion assay. Furthermore, PCR analysis of adhesin genes showed that 98.0% of these SEPEC strains tested positive for fimH, 69.4% for flu, 53.1% for csgA, 38.8% for mat, and 32.7% for iha. Analysis of the invasin genes showed that 16.3% of the SEPEC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Invasiveness; Escherichia coli; Sepsis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500007

Effect of photodynamic therapy on the extracellular matrix and associated components
Provedor de dados: 56
Autores: Pazos,M.d.C.; Nader,H.B.
In many countries, photodynamic therapy (PDT) has been recognized as a standard treatment for malignant conditions (for example, esophageal and lung cancers) and non-malignant ones such as age-related macular degeneration and actinic keratoses. The administration of a non-toxic photosensitizer, its selective retention in highly proliferating cells and the later activation of this molecule by light to form reactive oxygen species that cause cell death is the principle of PDT. Three important mechanisms are responsible for the PDT effectiveness: a) direct tumor cell kill; b) damage of the tumor vasculature; c) post-treatment immunological response associated with the leukocyte stimulation and release of many inflammatory mediators like cytokines, growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; Extracellular matrix; Adhesion; Chemical mediators; Matrix metalloproteinases.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800002

CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
Provedor de dados: 56
Autores: Aguiar,D.P.; Coelho-Aguiar,J.M.; Abreu,J.G.
Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teratocarcinoma; Adhesion; Condensation; CCN family; CCN2/CTGF; Extracellular matrix.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004

Further biochemical characterization of Mycobacterium leprae laminin-binding proteins
Provedor de dados: 56
Autores: Marques,M.A.M.; Mahapatra,S.; Sarno,E.N.; Santos,S.; Spencer,J.S.; Brennan,P.J.; Pessolani,M.C.V.
It has been demonstrated that the alpha2 chain of laminin-2 present on the surface of Schwann cells is involved in the process of attachment of Mycobacterium leprae to these cells. Searching for M. leprae laminin-binding molecules, in a previous study we isolated and characterized the cationic proteins histone-like protein (Hlp) and ribosomal proteins S4 and S5 as potential adhesins involved in M. leprae-Schwann cell interaction. Hlp was shown to bind alpha2-laminins and to greatly enhance the attachment of mycobacteria to ST88-14 Schwann cells. In the present study, we investigated the laminin-binding capacity of the ribosomal proteins S4 and S5. The genes coding for these proteins were PCR amplified and their recombinant products were shown to bind...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium leprae; Laminin; Adhesion; Schwann cell; Ribosomal protein; Histone.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400004

Adhesion of the human pathogen Sporothrix schenckii to several extracellular matrix proteins
Provedor de dados: 56
Autores: Lima,O.C.; Figueiredo,C.C.; Pereira,B.A.S.; Coelho,M.G.P.; Morandi,V.; Lopes-Bezerra,L.M.
The pathogenic fungus Sporothrix schenckii is the causative agent of sporotrichosis. This subcutaneous mycosis may disseminate in immunocompromised individuals and also affect several internal organs and tissues, most commonly the bone, joints and lung. Since adhesion is the first step involved with the dissemination of pathogens in the host, we have studied the interaction between S. schenckii and several extracellular matrix (ECM) proteins. The binding of two morphological phases of S. schenckii, yeast cells and conidia, to immobilized type II collagen, laminin, fibronectin, fibrinogen and thrombospondin was investigated. Poly (2-hydroxyethyl methacrylate) (poly-HEMA) was used as the negative control. Cell adhesion was assessed by ELISA with a rabbit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sporothrix schenckii; Fibronectin; Laminin; Type II collagen; Adhesion.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500020

Genomics and X-ray microanalysis indicate that Ca2+ and thiols mediate the aggregation and adhesion of Xylella fastidiosa
Provedor de dados: 56
Autores: Leite,B.; Ishida,M.L.; Alves,E.; Carrer,H.; Pascholati,S.F.; Kitajima,E.W.
The availability of the genome sequence of the bacterial plant pathogen Xylella fastidiosa, the causal agent of citrus variegated chlorosis, is accelerating important investigations concerning its pathogenicity. Plant vessel occlusion is critical for symptom development. The objective of the present study was to search for information that would help to explain the adhesion of X. fastidiosa cells to the xylem. Scanning electron microscopy revealed that adhesion may occur without the fastidium gum, an exopolysaccharide produced by X. fastidiosa, and X-ray microanalysis demonstrated the presence of elemental sulfur both in cells grown in vitro and in cells found inside plant vessels, indicating that the sulfur signal is generated by the pathogen surface....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Xylella fastidiosa; Aggregation; Adhesion; Methionine sulfoxide reductase; Biofilm.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600003

Alternagin-C, a disintegrin-like protein from the venom of Bothrops alternatus, modulates alpha2ß1 integrin-mediated cell adhesion, migration and proliferation
Provedor de dados: 56
Autores: Selistre-de-Araujo,H.S.; Cominetti,M.R.; Terruggi,C.H.B.; Mariano-Oliveira,A.; De Freitas,M.S.; Crepin,M.; Figueiredo,C.C.; Morandi,V.
The alpha2ß1 integrin is a major collagen receptor that plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. Alternagin-C (ALT-C), a disintegrin-like protein purified from the venom of the Brazilian snake Bothrops alternatus, competitively interacts with the alpha2ß1 integrin, thereby inhibiting collagen binding. When immobilized in plate wells, ALT-C supports the adhesion of fibroblasts as well as of human vein endothelial cells (HUVEC) and does not detach cells previously bound to collagen I. ALT-C is a strong inducer of HUVEC proliferation in vitro. Gene expression analysis was done using an Affimetrix HU-95A probe array with probe sets of ~10,000 human genes. In human fibroblasts growing on collagen-coated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha2ß1 integrin; Disintegrin; Snake venom; Adhesion; Gene expression; Extracellular matrix.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000007

Leukocyte adhesion - a fundamental process in leukocyte physiology
Provedor de dados: 56
Autores: Gahmberg,C.G.; Valmu,L.; Tian,L.; Kotovuori,P.; Fagerholm,S.; Kotovuori,A.; Kantor,C.; Hilden,T.
Leukocyte adhesion is of pivotal functional importance. The adhesion involves several different adhesion molecules, the most important of which are the leukocyte ß2-integrins (CD11/CD18), the intercellular adhesion molecules, and the selectins. We and others have extensively studied the specificity and binding sites in the integrins and the intercellular adhesion molecules for their receptors and ligands. The integrins have to become activated to exert their functions but the possible mechanisms of activation remain poorly understood. Importantly, a few novel intercellular adhesion molecules have been recently described, which seem to function only in specific tissues. Furthermore, it is becoming increasingly apparent that changes in integrins and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukocyte; Adhesion; Integrin; ICAM; Membrane; Glycoprotein.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500003

Growth kinetics of Escherichia coli O157:H7 on the epicarp of fresh vegetables and fruits
Provedor de dados: 58
Autores: Gullian-Klanian,Mariel; Sánchez-Solis,Maria José.
ABSTRACT Despite the increasing reports on the incidence of fresh vegetables and fruits as a possible vehicle for human pathogens, there is currently limited knowledge on the growth potential of Escherichia coli O157:H7 on different plant substrates. This study analyzed the selective adhesion and growth of E. coli O157:H7 on chili habanero (Capsicum chinense L.), cucumber (Cucumis sativus), radish (Raphanus sativus), tomato (Lycopersicon esculentum), beet (Beta vulgaris subsp. vulgaris), and onion (Allium cepa L.) under laboratory conditions. The Gompertz parameters were used to determine the growth kinetics. Scanning electron microscopy was used to visualize the adhesion of E. coli O157:H7 on the epicarp of the samples. Predictive models were constructed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E. coli O157:H7; Adhesion; Predictive growth; Low selectivity; Fruit and vegetables.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100104

Effects of growth temperature on polystyrene adhesion of Pseudomonas aeruginosa ATCC 27853
Provedor de dados: 58
Autores: Cappello,Simone; Guglielmino,Salvatore P.P.
The importance of avoiding bacterial adhesion to plastic medical devices is recognized. To better understand the interaction between bacteria and surface, we studied the influence of different growth temperatures (15, 30 and 47ºC) on the polystyrene adhesion capacity of Pseudomonas aeruginosa ATCC 27853.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pseudomonas aeruginosa; Temperature; Hydrophobicity; Adhesion.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300001

Use of Pseudomonas stutzeri and Candida utilis in the improvement of the conditions of Artemia culture and protection against pathogens
Provedor de dados: 58
Autores: Abdelkarim,Mahdhi; Kamel,Chaieb; Fathi,Kammoun; Amina,Bakhrouf.
To evaluate the effect of two bacterial strains isolated from Artemia cysts and yeast (Candida utilis) on the survival, growth and total biomass production of its larvae, challenge tests were performed with Candida utilis, Pseudomonas stutzeri and Pasteurella haemolityca. In addition, a pathogenic strain of Vibrio alginolyticus was tested for comparative purposes. Pseudomonas stutzeri and Candida utilis have no impact on survival, but enhance growth and total biomass production of the larvae. However, we noted that Pasteurella haemolityca affect negatively Artemia larvae. The adhesion and antagonism assay demonstrates that Candida utilis and Pseudomonas stutzeri are fairly adherent and play an important role in the enhancement of the protection of Artemia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemia; Probiotics; Adhesion; Yeast; Bacteria.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100017

Architecture and relevance of several strongly adhered biofilms over a polyester imide (PEI) surface
Provedor de dados: 58
Autores: Soncini-Júnior,Giancarlo; Franchetti,Sandra Mara Martins; Marconato,José Carlos.
A microscopical study was undertaken to analyze the eventual fungi adhesion over a polyester imide surface in enameled copper wires. Scanning electron microscopy allowed to observe in these adhered biofilms, a high amount of pigments, hyphae and its enzymatic arsenal probably acting in the surface of the polymer. Due to the highly aromatic nature of this polyester and the residual phenol derivates still present in the reticulated polymer, an antifungal activity could be expected, but no significant changes were noted in fungal growing and in the adhesion process. Additionally, the wires showed completely failure in all insulation properties. The studies aimed to understand and evaluate the great fungi potential that could be explored in biodeterioration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungi; Biofilms; Hyphae; Pigments; Adhesion; Scanning electron microscopy; Polymeric surface; Polyester-imide; Enameled copper wires.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500036

Adhesion of Salmonella Enteritidis to stainless steel surfaces
Provedor de dados: 58
Autores: Oliveira,Kelly; Oliveira,Tereza; Teixeira,Pilar; Azeredo,Joana; Oliveira,Rosário.
Adhesion of microorganisms to food processing surfaces and the problems it causes are a matter of strong concern to the food industry. Contaminated food processing surfaces may act as potential sources of transmission of pathogens in food industry, catering and in the domestic environments. Several studies have shown that adhesion of bacteria to surfaces partly depends upon the nature of the inert surfaces and partly upon the bacterial surface properties. The aim of this study was to compare the adhesion of four different strains of Salmonella Enteritidis to stainless steel 304 (SS 304). The effect of surface hydrophobicity and surface elemental composition on the adhesion process was also analysed. Hydrophobicity was evaluated through contact angle...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Salmonella Enteritidis; Hydrophobicity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200026

Curli fimbria: an Escherichia coli adhesin associated with human cystitis
Provedor de dados: 58
Autores: Cordeiro,Melina Aparecida; Werle,Catierine Hirsch; Milanez,Guilherme Paier; Yano,Tomomasa.
Abstract Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curli fimbria; Adhesion; Escherichia coli; UPEC.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200414

Assessment of hydrophobicity and roughness of stainless steel adhered by an isolate of Bacillus cereus from a dairy plant
Provedor de dados: 58
Autores: Bernardes,Patrícia Campos; Andrade,Nélio José de; Ferreira,Sukarno Olavo; Sá,João Paulo Natalino de; Araújo,Emiliane Andrade; Delatorre,Deyse Maria Zanom; Luiz,Lívia Maria Pinheiro.
The interaction between the surface of stainless steel and Bacillus cereus was studied in terms of the characteristics of interfacial interaction determined from the measurement of the contact angle of the surface of B. cereus and stainless steel in the presence or absence of B. cereus adherence. The microtopographies and the roughness of the surface of stainless steel and stainless steel adhered by B. cereus were evaluated with the help of atomic force microscopy and perfilometry. The strain of B. cereus studied was considered hydrophilic, whereas the stainless steel was considered hydrophobic. The adhesion was not thermodynamically favorable (ΔGadhesion &gt; 0) between the stainless steel and the strain of B. cereus studied. Thus, the interaction between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrophobicity; Roughness; Bacillus cereus; Dairy plant; Adhesion.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400017

Adhesion and biocides inactivation of Salmonella on stainless steel and polyethylene
Provedor de dados: 58
Autores: Tondo,Eduardo Cesar; Machado,Taís Raquel Marcon; Malheiros,Patrícia da Silva; Padrão,Débora Kruger; Carvalho,Ana Lyl de; Brandelli,Adriano.
The adhesion of Salmonella (S.) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis, S. Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA), sodium hypochlorite (NaOCl), and quaternary ammonium (Quat) sanitizers. Hydrophobicity of the surfaces was evaluated by contact angle measurements using the sessile drop method and bacterial adhesion was accompanied through bacterial counts and scanning electron microscopy (SEM). Results indicated that the three serovars of Salmonella presented similar adhesion to both materials...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Adhesion; Biocides; Stainless steel; Polyethylene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400022

Adhesion and invasion of Clostridium perfringens type A into epithelial cells
Provedor de dados: 58
Autores: Llanco,Luis A.; Nakano,Viviane; Moraes,Claudia T.P. de; Piazza,Roxane M.F.; Avila-Campos,Mario J.
ABSTRACT Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and α- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated. Six (27.3%) of the 22 C. perfringens type A strains were harboring the tpeL gene and produced morphological alterations in Vero cells after 6 h of incubation. Strains tpeL (-) induced strong cell rounding after 6 h of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Invasion; Clostridium perfringens type A; Necrotic enteritis; TpeL gene.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400764

Hyaluronic acid and synovial fluid in preventing adhesion formation after tenorrhaphy: An in vivo study on rabbit Achilles tendon
Provedor de dados: 65
Autores: Kurt,Başak; Ozaydin,Isa; Sozmen,Mahmut; Bilge,Ali; Cihan,Mete; Hamamci,Emre; Aydin,Ugur.
ABSTRACT:  Peritendinous adhesions are a significant problem in tendon surgery. One of the most preferred methods for preventing adhesion formation is anti-adhesive applications. This study aimed to investigate the efficacy of natural synovial fluid as an alternative viscoelastic agent for preventing adhesion in the healing period compared to hyaluronic acid. Thirty adult New Zealand rabbits were used in the study to form three experimental models. In all groups, a rupture was induced in the Achilles tendons. Following tenorrhaphy, hyaluronic acid was applied in one group and synovial fluid in the other group for anti-adhesive purposes. In the control group, no viscoelastic application was made. Bandage was applied to the operated extremities for three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Achilles tendon; Adhesion; Synovial fluid; Hyaluronic acid; Rabbit.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700601