Consulta: subjectFacets:"AgNOR"
Registros recuperados: 12
Data/hora: 06/06/2024 01:11:02

Karyotypic conservatism in five species of Prochilodus (Characiformes, Prochilodontidae) disclosed by cytogenetic markers
Provedor de dados: 74
Autores: Voltolin,Tatiana Aparecida; Penitente,Manolo; Mendonça,Bruna Bueno; Senhorini,José Augusto; Foresti,Fausto; Porto-Foresti,Fábio.
The family Prochilodontidae is considered a group with well conserved chromosomes characterized by their number, morphology and banding patterns. Thence, our study aimed at accomplishing a cytogenetic analysis with conventional methods (Giemsa staining, silver staining of the nucleolus organizer regions-AgNOR, and C-banding) and fluorescence in situ hybridization (FISH) with 18S and 5S ribosomal DNA probes in five species of the Prochilodus genus (Prochilodus argenteus, Prochilodus brevis, Prochilodus costatus, Prochilodus lineatus and Prochilodus nigricans) collected from different Brazilian hydrographic basins. The results revealed conservatism in chromosome number, morphology, AgNORs 18S and 5S rDNAs location and constitutive heterochromatin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgNOR; C-banding; Hydrographic basins; Conserved karyotype; FISH.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300008

Cytogenetic characterization of a population of Bryconamericus aff. iheringii (Characidae,Tetragonopterinae)
Provedor de dados: 74
Autores: Paintner-Marques,Tânia Regina; Giuliano-Caetano,Lucia; Dias,Ana Lúcia.
A cytogenetic analysis of thirteen specimens of Bryconamericus aff. Iheringii revealed a diploid number of 52 chromosomes and a karyotype of 8M+22SM+10ST+12A with a fundamental number (FN) of 92. The nucleolus organizer regions (NORs) were studied by means of AgNO3 and CMA3 staining as well as by FISH using an 18S rDNA probe. NORs were found to be located at the terminal position on the short arm of the submetacentric chromosome pair. C-banding showed strong telomeric and centromeric staining in the majority of the chromosomes, and a similar pattern was observed after treatment with AluI restriction enzyme
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgNOR; Alu I; Bryconamericus; C-banding; CMA3; RDNA 18S.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200007

Comparative cytogenetics in different populations of the cavernicolous diplopod Pseudonannolene strinatii (Diplopoda, Pseudonannolenidae)
Provedor de dados: 76
Autores: Campos,Kleber A.; Fontanetti,Carmem S.
Different populations of Pseudonannolene strinatii Mauriès, 1974 collected from three caves in Iporanga, state of São Paulo, were cytogenetically compared using techniques of conventional coloration, C-banding and silver nitrate impregnation. Specimens were morphologically similar and small cytogenetic differences were observed between the populations with relation to the distribution of constitutive heterochromatin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Millipedes; C-banding; Karyotype; AgNOR; Chromosomes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212013000100006

Diversity and chromosomal evolution in the genusAncistrusKner, 1854 (Loricariidae: Ancistrini) from three hydrographic basins of Mato Grosso State, Brazil
Provedor de dados: 83
Autores: Mariotto,Sandra; Centofante,Liano; Moreira-Filho,Orlando.
Cytogenetic analyses were carried out in 117 specimens of seven species of the genus Ancistrus from three hydrographic in Mato Grosso State: Paraguay, Araguaia-Tocantins and Amazon basins. Conventional cytogenetic techniques were used to obtain mitotic chromosomes. C-banding was performed to detect heterochromatic regions and silver nitrate staining was used to identify nucleolar organizer regions (Ag-NORs). The counted and paired chromosomes revealed diploid numbers ranging from 2n = 40 to 2n = 54 with karyotype formulae varying from FN = 80 to FN = 86. Single marks in distinct chromosomes identified the nucleolar organizer regions. The constitutive heterochromatin was scarce in the diploid number from 2n = 50 to 2n = 54 and conspicuous blocks were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgNOR; Cytogenetic; Diploid number; Heterochromatin.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100125

Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus
Provedor de dados: 42
Autores: GOSSLER,VANESSA S.A.; SANTOS,FRANCISLAINE A.G.; AZEVEDO,ALINE R. DE; GONÇALVES,PRISCILA C.; RIGOLO,HUGO A.; TREVISAN,CLEONICE; MASSENO,ANA PAULA B.; CARDOSO,ANA PAULA M.M.; PAPA,PAULA C.; CASTILHO,CALIÊ; GIOMETTI,INES C.
ABSTRACT This study aimed to determine the histological features of the endometrium of bitches, as well as the cell proliferation at specific moments of diestrus, 10, 20, 30, 40, 50 and 60 days post ovulation, correlating the endometrial thickness with the uterine cell proliferation and the metabolic state (weight, blood glucose and plasma cholesterol) of the animals. Therefore, the right and left uterine horns of 26 clinically healthy bitches submitted to ovariohysterectomy were histologically analyzed 10, 20, 30, 40, 50 and 60 days post ovulation. The hematoxylin-eosin and AgNOR staining techniques were performed. All parameters were evaluated by ANOVA and post-hoc Tukey test (p&lt;0.05). The correlation between endometrial thickness and uterine cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgNOR; Dogs; Hematoxylin-eosin; Uterus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401719

Mastocitoma cutâneo canino: estudo de 45 casos
Provedor de dados: 45
Autores: Rech,R.R.; Graça,D.L.; Kommers,G.D.; Sallis,E.S.V.; Raffi,M.B.; Garmatz,S.L.
Quarenta e cinco mastocitomas cutâneos caninos foram graduados histologicamente com o uso de hematoxilina-eosina. Foram empregados os métodos azul de toluidina e região organizadora nucleolar argirofílica (AgNOR) para, respectivamente, evidenciar os grânulos citoplasmáticos e avaliar o índice de proliferação celular. Diversas características histológicas foram observadas, como distribuição das células na pele, tamanho, forma, aspecto de citoplasma e núcleo, quantidade de estroma, presença de eosinófilos e alterações associadas. Com base nessas caracteríscas, 37,8% dos mastocitomas foram classificados como grau I, 51,1% como grau II e 11,1% como grau III. A média geral de AgNOR nos mastocitomas foi de 1,9 (1,2 a 4,3) e as médias para os graus I, II e III...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cão; Mastocitoma; AgNOR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000400004

Association of nucleolar organizing regions and Ki-67 expression with recurrence rate of hair follicle tumor in dogs
Provedor de dados: 45
Autores: Souza,P.C.; Ocarino,N.M.; Tavares,W.L.F.; Boeloni,J.N.; Cassali,G.D.; Serakides,R.
Mitotic index, nuclear diameter, number of nucleolar organizing regions, and Ki-67 expression, in hair follicle tumors of 82 dogs were evaluated. Tissue specimens were used to prepare sections for histological staining for number of nucleolar organizing region and immunohistochemical staining for Ki-67. Tumors were classified as trichoblastoma (n=32), benign trichoepithelioma (n=30), pilomatricoma (n=7), malignant trichoepithelioma (n=6), infundibular keratinizing acanthoma (n=5), and tricholemmoma (n=2). Head, dorsum, and limbs were the most affected sites. Malignant trichoepithelioma presented significantly higher mitotic index, number of nucleolar organizing regions and Ki-67 expression. Regarding benign neoplasms, trichoblastoma presented significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Neoplasm; AgNOR; Hair follicle; Ki-67.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000500006

Cell proliferation markers in the transplanted canine transmissible venereal tumor
Provedor de dados: 45
Autores: Santos,F.G.A.; Moro,L.; Cassali,G.D.; Paixão,T.A.; Campos,P.P.; Silva,S.S.; Vasconcelos,A.C.
Adult male mongrel dogs were subcutaneously transplanted with the canine transmissible venereal tumor (TVT) on the hypogastric region. Twelve specimens of tumors were collected, half during the proliferative phase and the other half during the regressive phase. Fragments of the tumor were fixed in 10% buffered formalin and routinely processed for light microscopy. Sections of 4µm were stained by Schorr or AgNOR or either immunostained for MIB1 (Ki67). Schorr stain, AgNOR and MIB1 showed an increased proliferative activity through mitotic index, nuclear argyrophilic protein stain and cycling tumoral cells in the growing tumors, respectively. All of the three cell proliferation markers were able to distinguish the TVT in both evolution phases. MIB1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Venereal tumors; MIB1; AgNOR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600010

Marcadores de proliferação celular na avaliação do crescimento do tumor sólido e ascítico de Ehrlich
Provedor de dados: 45
Autores: Silva,A.E.; Santos,F.G.A.; Cassali,G.D.
Twenty BALB/c mice were inoculated with cell suspension of Ehrlich tumor. Ten mice were inoculated in the cushion plant (solid form) and the other 10 in the peritoneum (ascitic form). Animals were euthanized on different times (7 and 14 days). Cytological and histological slides, immunohistochemical (PCNA) analysis and NORs silver impregnation technique were performed. The results showed more proliferation on the 7th day in the ascitic form and on 14th day in the solid form, using both analyses (PCNA and AgNORs). The alterations observed in the Ehrlich tumor’s proliferation activity suggested that the growth curve is different between ascitic and solid forms. In the first one, the proliferation peak occurs on the seventh day and in the solid tumor the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Camundongo; Tumor de Ehrlich; AgNOR; PCNA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400030

Tratamento de feridas excisionais de coelhos com extrato de barbatimão associado a células mononucleares autólogas da medula óssea
Provedor de dados: 45
Autores: Rodrigues,D.F.; Mendes,F.F.; Menezes,L.B.; Carvalho,W.L.; Sá,S.; Silva,J.A.; Souza,L.A.; Silva,L.A.F.
RESUMO Objetivou-se com este estudo avaliar o processo de cicatrização de feridas de coelhos tratadas com extrato de barbatimão (S. adstringens) associado a células mononucleares autólogas da medula óssea (CMMO). Utilizaram-se 20 coelhos, distribuídos em quatro grupos: B, extrato de barbatimão; CB, CMMO com extrato de barbatimão; CS, CMMO com solução fisiológica; S, solução fisiológica. Foi avaliada a presença de crosta, hiperemia, secreção, hemorragia, reepitelização, área da ferida e tempo de cicatrização. No terceiro, sétimo, 14º e 21º dias pós-operatório, realizou-se a biópsia das feridas e avaliaram-se os indicadores dos processos de inflamação e de reparo, com destaque para o colágeno, na coloração picrosírius, bem como de proliferação celular, na...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgNOR; Cicatrização; Colágeno; Stryphnodendron adstringens; Terapia celular.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501243

Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver
Provedor de dados: 47
Autores: Martín,Carlos A.; Surur,José Miguel; García,Marcela N.; Corrons,Félix; Badrán,Amado F.
Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 ± 16.1 SE; AgNORs 77.3 ± 3.4 SE) located at 16:00/54 Time of Day / Hours Post- Hepatectomy (TD/HPH), which were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver regeneration; Sinusoid littoral cell; Bromodeoxyuridine; AgNOR; Immunohistochemistry.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100005

Interindividual size heteromorphism of NOR and chromosomal location of 5S rRNA genes in Iheringichthys labrosus
Provedor de dados: 52
Autores: Carvalho,Rafael Augusto de; Dias,Ana Lúcia.
Twenty-five specimens of Iheringichthys labrosus from the Capivara Reservoir were analysed cytogenetically. AgNORs were detected in a pair of ST chromosomes, in the telomeric region of the long arm. Some individuals showed size heteromorphism of this region between homologous chromosomes. Treatment with CMA3 displayed GC-rich regions corresponding to the AgNOR pair, plus other fluorescent staining. In situ hybridization by fluorescence (FISH) with the 18S rDNA probe showed only one pair of stained chromosomes, confirming the heteromorphism observed with AgNO3 and CMA3 in some individuals. The 5S rDNA probe revealed telomeric staining on the long arm of a pair of chromosomes of the ST-A group, probably different from the NOR pair.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish chromosome; AgNOR; In situ hibridization; Chromomycin A3; Heteromorphism.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100017