Consulta: subjectFacets:"Alanine"
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Data/hora: 03/06/2024 11:09:10

Effect of sediment nearness on the metabolic enzyme activity and energy state of the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Le Moullac, Gilles; Fleury, Pierre-gildas; Le Coz, Jean-rene; Moal, Jeanne; Samain, Jean-francois.
This study was designed to assess in situ the effects of sediment nearness on Crassostrea gigas metabolism. One year-old oysters were reared for 5 months (April-August) in plastic bags on metallic frames at 10 and 60 cm off the sediment at the beginning of April. The management of the energetic resources (storage, consumption) was estimated with respect to biochemical changes of proteins, lipids and carbohydrates. In order to evaluate the  effect of the sediment nearness at cellular level, the metabolic rate was evaluated by assessing pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK) enzyme activities and electron transport system (ETS) activity. The metabolic pathways were assessed by measuring ATP and intermediary metabolites such as...
Tipo: Text
Palavras-chave: Aspartate; Succinate; Alanine; ETS; PEPCK; PK; Carbohydrate; Lipids; Proteins; Sediment; Oyster; Aspartate; Succinate; Alanine; ETS; PEPCK; PK; Glucides; Lipides; Proteines; Sédiment; Huître.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2803.pdf

Ecophysiological and metabolic adaptations to sulphide exposure of the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Le Moullac, Gilles; Cheize, Marie; Gastineau, Olivier; Daniel, Jean-yves; Le Coz, Jean-rene; Huvet, Arnaud; Moal, Jeanne; Pouvreau, Stephane; Van Wormhoudt, A; Samain, Jean-francois.
The purpose of this study is to examine the response of the oyster Crassostrea gigas to sulphide at different biological scales. A first experiment was designed to measure for 20 h the clearance (CR) and oxygen consumption (OC) rates of oysters exposed at different concentrations of sulphide (0, 3, 6, 12, 20, and 40 mu M). The second experiment was carried out to evaluate the metabolic adaptations to chronic sulphide exposure for 10 days by measuring PK and PEPCK enzyme activities, succinate and alanine content, the adenylate energy charge (AEC), and the activity of the electron transport system (ETS)in whole oysters at above 20 mu M. PK and PEPCK mRNAs and enzyme activities have been measured in adductor muscle. When exposed to sulphide above 20 mu M, CR...
Tipo: Text
Palavras-chave: ATP; AEC; Electron transport system; Succcinate; Alanine; Phosphoenolpyruvate carboxykinase; Pyruvate kinase; Oxygen consumption; Clearance rate; Crassostrea gigas; Sulphide; Oyster.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3924.pdf

Metabolic adjustments in the oyster Crassostrea gigas according to oxygen level and temperature
Provedor de dados: 5
Autores: Le Moullac, Gilles; Queau, Isabelle; Le Souchu, Pierrick; Pouvreau, Stephane; Moal, Jeanne; Le Coz, Jean-rene; Samain, Jean-francois.
The purpose of this study was to examine the responses of the oyster C. gigas to oxygen levels at subcellular and whole-organism levels. Two experiments were carried out. The first experiment was designed to measure at 15°C, 20°C and 25°C for 20 hours the clearance (CR) and oxygen consumption (OC) rates of oysters exposed at different concentrations of oxygen. The goal of this first part was to estimate the hypoxic threshold for oysters below which their metabolism shifts toward anaerobiosis, by estimating the oxygen critical point (PcO2) at 15, 20 and 25°C. The second experiment was carried out to evaluate the metabolic adaptations to hypoxia for 20 days at three temperatures: 12°C, 15°C and 20°C. The metabolic pathways were characterized by the...
Tipo: Text
Palavras-chave: Succinate; Alanine; Phosphoenolpyruvate carboxykinase; Pyruvate kinase; Temperature; Hypoxia; Oysters.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2693.pdf

Differences in ureide and amino acid content of water stressed soybean inoculated with Bradyrhizobium japonicum and B. elkanii
Provedor de dados: 86
Autores: Ramos,Maria Lucrecia Gerosa; Parsons,Richard; Sprent,Janet Irene.
The objective of this work was to study the response to water stress of a drought sensitive soybean cultivar inoculated with Bradyrhizobium japonicum (strain CB1809, Semia 586) and B. elkanii (strain 29W, Semia 5019). CB1809 nodulated plants produced a significantly higher root fraction (19%) than 29W (14.6%). Plants inoculated with CB1809 produced less nodules and accumulated more nitrogen than those inoculated with 29W. In general, low amounts of ureides in nodules were found in watered plants inoculated with either CB1809 or 29W strains, but those levels were five-fold increased in stressed plants inoculated with CB1809. Nodules formed by strain CB1809 had aspartate and glutamate as major amino acids, while those formed by 29W had glutamate, asparagine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Alanine; Asparagine; Aspartate; Glutamate; Nitrogen fixation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000500005

Effect of salt on the growth and metabolism of Glycine max
Provedor de dados: 52
Autores: Queiroz,Helena Müller; Sodek,Ladaslav; Haddad,Claudia Regina Baptista.
Soybean plants cultivated with 50, 100 and 200 mM of NaCl, revealed that root growth was less affected by salinity than shoots. Salinity led to a reduction in leaf area and an increase in water content of the roots. These factors could contribute to the adaptation of the plant, improving its hydration. Although nitrate and free amino acid levels were reduced by salt treatment in roots, protein content of leaves was not altered. Salinity led to alterations in xylem amino acid composition, with increases in Ser, Ala, Gaba and Pro and a decrease in Asn. Similar changes were seen for Asn and Ser in roots together with a much stronger increase in Gaba. It is suggested that the decline in Asn reflects its conversion to Ala and Gaba (via Glu) in the roots while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Stress; Amino acid transport; Asparagine; Alanine; Γ-aminobutyrate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600002

Amino acid and ureide transport in the xylem of symbiotic soybean plants during short-term flooding of the root system in the presence of different sources of nitrogen
Provedor de dados: 61
Autores: Thomas,André Luís; Sodek,Ladaslav.
The transport of organic N compounds to the shoot in the xylem sap of nodulated soybean plants was investigated in an attempt to better understand the changes in N metabolism under root hypoxia (first 5 days of flooding), with different sources of N in the medium. NO3- is beneficial for tolerance of plants to waterlogging, whereas other N sources such as NH4+ and NH4NO3, are not. Nevertheless, in the presence of NH4+ high levels of amino acids were transported in the xylem, consistent with its assimilation. Some increase in the transport of amino acids was also seen with NO3- nutrition during waterlogging, but not with N-free medium. Ureide transport in the xylem was severely reduced during waterlogging, consistent with impaired N2 fixation under these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Alanine; Hypoxia; N metabolism.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200010