Consulta: subjectFacets:"Animal models"
Registros recuperados: 13
Data/hora: 12/06/2024 08:33:51

Effects of gene therapy on cardiovascular symptoms of lysosomal storage diseases
Provedor de dados: 74
Autores: Poletto,Edina; Pasqualim,Gabriela; Giugliani,Roberto; Matte,Ursula; Baldo,Guilherme.
Abstract Lysosomal storage diseases (LSDs) are inherited conditions caused by impaired lysosomal function and consequent substrate storage, leading to a range of clinical manifestations, including cardiovascular disease. This may lead to significant symptoms and even cardiac failure, which is an important cause of death among patients. Currently available treatments do not completely correct cardiac involvement in the LSDs. Gene therapy has been tested as a therapeutic alternative with promising results for the heart disease. In this review, we present the results of different approaches of gene therapy for LSDs, mainly in animal models, and its effects in the heart, focusing on protocols with cardiac functional analysis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lysosomal storage disease; Gene therapy; Cardiovascular disease; Animal models; Heart.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200261

Animal Models of Nutritional Induction of Type 2 Diabetes Mellitus
Provedor de dados: 77
Autores: Barbosa-da-Silva,Sandra; Sarmento,Isabele Bringhenti; Bargut,Thereza C. Lonzetti; Souza-Mello,Vanessa; Aguila,Marcia Barbosa; Mandarim-de-Lacerda,Carlos A.
Type 2 diabetes mellitus (DM2) is the most common chronic metabolic disease, affecting approximately 6% of the adult population in the Western world. This condition is a major cause of cardiovascular disease, blindness, renal failure, and amputations, with increasing prevalence worldwide. The inuence of obesity on type 2 diabetes risk is determined by the degree of obesity and by body fat localization, with insulin resistance (IR) being the main link between these metabolic diseases. Experimental studies have shown that dietary factors, and particularly lipids, are strongly positively associated with body mass (BM) gain; IR; and, consequently, type 2 diabetes. Similarly, excessive consumption of energy-dense carbohydrate-rich foods can trigger the onset of...
Tipo: Journal article
Palavras-chave: Animal models; Nutritional induction; Metabolic disease; Type 2 diabetes mellitus.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100046

Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of rats
Provedor de dados: 56
Autores: Samano,M.N.; Pazetti,R.; Prado,C.M.; Tibério,I.C.; Saldiva,P.H.N.; Moreira,L.F.P.; Pêgo-Fernandes,P.M.; Jatene,F.B.; Das-Neves-Pereira,J.C.
Pneumonectomy is associated with high mortality and high rates of complications. Postpneumonectomy pulmonary edema is one of the leading causes of mortality. Little is known about its etiologic factors and its association with the inflammatory process. The purpose of the present study was to evaluate the role of pneumonectomy as a cause of pulmonary edema and its association with gas exchange, inflammation, nitric oxide synthase (NOS) expression and vasoconstriction. Forty-two non-specific pathogen-free Wistar rats were included in the study. Eleven animals died during or after the procedure, 21 were submitted to left pneumonectomy and 10 to sham operation. These animals were sacrificed after 48 or 72 h. Perivascular pulmonary edema was more intense in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pneumonectomy; Pulmonary edema; Nitric oxide synthase; Animal models; NOS.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100019

Effect of acute treatment with a water-alcohol extract of Erythrina mulungu on anxiety-related responses in rats
Provedor de dados: 56
Autores: Onusic,G.M.; Nogueira,R.L.; Pereira,A.M.S.; Viana,M.B.
We investigated the effect of acute oral treatment with a water-alcohol extract of the inflorescence of Erythrina mulungu (EM, Leguminosae-Papilionaceae) (100, 200 and 400 mg/kg) on rats submitted to different anxiety models: the elevated T-maze (for inhibitory avoidance and escape measurements), the light/dark transition, and the cat odor test. These models were selected for their presumed capacity to demonstrate specific subtypes of anxiety disorders as recognized in clinical practice. Treatment with 200 mg/kg EM impaired avoidance latencies (avoidance 1 - 200 mg/kg EM: 18 ± 7 s, control group: 40 ± 9 s; avoidance 2 - 200 mg/kg EM: 15 ± 4 s, control group: 110.33 ± 38 s) in a way similar to the reference drug diazepam (avoidance 1: 3 ± 0.79 s; avoidance...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Erythrina mulungu; Anxiety; Animal models.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400011

Experimental models in vaccine research: malaria and leishmaniasis
Provedor de dados: 56
Autores: Teixeira,C.; Gomes,R.
Animal models have a long history of being useful tools, not only to test and select vaccines, but also to help understand the elaborate details of the immune response that follows infection. Different models have been extensively used to investigate putative immunological correlates of protection against parasitic diseases that are important to reach a successful vaccine. The greatest challenge has been the improvement and adaptation of these models to reflect the reality of human disease and the screening of vaccine candidates capable of overcoming the challenge of natural transmission. This review will discuss the advantages and challenges of using experimental animal models for vaccine development and how the knowledge achieved can be extrapolated to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human vaccines; Animal models; Malaria; Leishmaniasis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200109

Animal models of prenatal immune challenge and their contribution to the study of schizophrenia: a systematic review
Provedor de dados: 56
Autores: Macêdo,D.S.; Araújo,D.P.; Sampaio,L.R.L.; Vasconcelos,S.M.M.; Sales,P.M.G.; Sousa,F.C.F.; Hallak,J.E.; Crippa,J.A.; Carvalho,A.F.
Prenatal immune challenge (PIC) in pregnant rodents produces offspring with abnormalities in behavior, histology, and gene expression that are reminiscent of schizophrenia and autism. Based on this, the goal of this article was to review the main contributions of PIC models, especially the one using the viral-mimetic particle polyriboinosinic-polyribocytidylic acid (poly-I:C), to the understanding of the etiology, biological basis and treatment of schizophrenia. This systematic review consisted of a search of available web databases (PubMed, SciELO, LILACS, PsycINFO, and ISI Web of Knowledge) for original studies published in the last 10 years (May 2001 to October 2011) concerning animal models of PIC, focusing on those using poly-I:C. The results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schizophrenia; Animal models; Neurodevelopment; Prenatal immune activation; Poly-I:C.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300001

Transgenic animal models for the functional analysis of vasoactive peptides
Provedor de dados: 56
Autores: Bader,M.
The interplay of vasoactive peptide systems is an essential determinant of blood pressure regulation in mammals. While the endothelin and the renin-angiotensin systems raise blood pressure by inducing vasoconstriction and sodium retention, the kallikrein-kinin and the natriuretic-peptide systems reduce arterial pressure by eliciting vasodilatation and natriuresis. Transgenic technology has proven to be very useful for the functional analysis of vasoactive peptide systems. As an outstanding example, transgenic rats overexpressing the mouse Ren-2 renin gene in several tissues become extremely hypertensive. Several other transgenic rat and mouse strains with genetic modifications of components of the renin-angiotensin system have been developed in the past...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin-angiotensin system; Transgenic; Animal models; Knockout.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900011

An update on the tools for creating transgenic animal models of human diseases – focus on atherosclerosis
Provedor de dados: 56
Autores: Volobueva,A.S.; Orekhov,A.N.; Deykin,A.V.
Animal models of diseases are invaluable tools of modern medicine. More than forty years have passed since the first successful experiments and the spectrum of available models, as well as the list of methods for creating them, have expanded dramatically. The major step forward in creating specific disease models was the development of gene editing techniques, which allowed for targeted modification of the animal's genome. In this review, we discuss the available tools for creating transgenic animal models, such as transgenesis methods, recombinases, and nucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and CRISPR/Cas9 systems. We then focus specifically on the models of atherosclerosis, especially...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal models; Gene editing; Atherosclerosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500301

Pamidronate for the treatment of osteoporosis secondary to chronic cholestatic liver disease in Wistar rats
Provedor de dados: 56
Autores: Pereira,F.A.; Mattar,R.; Facincani,I.; Defino,H.L.A.; Ramalho,L.N.Z.; Jorgetti,V.; Volpon,J.B.; Paula,F.J.A. de.
Osteoporosis is a major complication of chronic cholestatic liver disease (CCLD). We evaluated the efficacy of using disodium pamidronate (1.0 mg/kg body weight) for the prevention (Pr) or treatment (Tr) of cholestasis-induced osteoporosis in male Wistar rats: sham-operated (Sham = 12); bile duct-ligated (Bi = 15); bile duct-ligated animals previously treated with pamidronate before and 1 month after surgery (Pr = 9); bile duct-ligated animals treated with pamidronate 1 month after surgery (Tr = 9). Rats were sacrificed 8 weeks after surgery. Immunohistochemical expression of IGF-I and GH receptor was determined in the proximal growth plate cartilage of the left tibia. Histomorphometric analysis was performed in the right tibia and the right femur was used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic osteodystrophy; Osteoporosis pathogenesis; Growth factors; Biomechanics; Animal models; Pamidronate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200021

The relationship between anxiety and depression in animal models: a study using the forced swimming test and elevated plus-maze
Provedor de dados: 56
Autores: Andreatini,R.; Bacellar,L.F.S.
The present study evaluated the correlation between the behavior of mice in the forced swimming test (FST) and in the elevated plus-maze (PM). The effect of the order of the experiments, i.e., the influence of the first test (FST or PM) on mouse behavior in the second test (PM or FST, respectively) was compared to handled animals (HAND). The execution of FST one week before the plus-maze (FST-PM, N = 10), in comparison to mice that were only handled (HAND-PM, N = 10) in week 1, decreased % open entries (HAND-PM: 33.6 ± 2.9; FST-PM: 20.0 ± 3.9; mean ± SEM; P&amp;lt;0.02) and % open time (HAND-PM: 18.9 ± 3.3; FST-PM: 9.0 ± 1.9; P&amp;lt;0.03), suggesting an anxiogenic effect. No significant effect was seen in the number of closed arm entries (FST-PM: 9.5...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anxiety; Depression; Comorbidity; Animal models.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900011

Pitfalls in the assessment of murine atherosclerosis
Provedor de dados: 56
Autores: Catanozi,S.; Rocha,J.C.; Passarelli,M.; Chiquito,F.C.; Quintão,E.C.R.; Nakandakare,E.R.
This review provides examples of the fact that different procedures for the measurement of atherosclerosis in mice may lead to interpretation of the extent of atherosclerosis having markedly different biological and clinical significance for humans: 1) aortic cholesterol measurement is highly sensitive for the detection of early and advanced atherosclerosis lesions, but misses the identification of the location and complexity of these lesions that are so critical for humans; 2) the histological analysis of the aortic root lesions in simvastatin-treated and control mice reveals similar lesion morphology in spite of the remarkable simvastatin-induced reduction of the aortic cholesteryl ester content; 3) in histological analyses, chemical fixation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Animal models; Disease models; Aorta; Pathology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600001

The brain decade in debate: II. Panic or anxiety? From animal models to a neurobiological basis
Provedor de dados: 56
Autores: Andreatini,R.; Blanchard,C.; Blanchard,R.; Brandão,M.L.; Carobrez,A.P.; Griebel,G.; Guimarães,F.S.; Handley,S.L.; Jenck,F.; Leite,J.R.; Rodgers,J.; Schenberg,L.C.; Da Cunha,C.; Graeff,F.G.
This article is a transcription of an electronic symposium sponsored by the Brazilian Society of Neuroscience and Behavior (SBNeC). Invited researchers from the European Union, North America and Brazil discussed two issues on anxiety, namely whether panic is a very intense anxiety or something else, and what aspects of clinical anxiety are reproduced by animal models. Concerning the first issue, most participants agreed that generalized anxiety and panic disorder are different on the basis of clinical manifestations, drug response and animal models. Also, underlying brain structures, neurotransmitter modulation and hormonal changes seem to involve important differences. It is also common knowledge that existing animal models generate different types of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panic; Anxiety; Fear; Anxiolytics; 5-HT; Benzodiazepines; Serotonin; Amygdala; Periaqueductal gray matter; Animal models.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200001

Experimental model of intervertebral disc degeneration by needle puncture in Wistar rats
Provedor de dados: 56
Autores: Issy,A.C.; Castania,V.; Castania,M.; Salmon,C.E.G.; Nogueira-Barbosa,M.H.; Bel,E. Del; Defino,H.L.A.
Animal models of intervertebral disc degeneration play an important role in clarifying the physiopathological mechanisms and testing novel therapeutic strategies. The objective of the present study is to describe a simple animal model of disc degeneration involving Wistar rats to be used for research studies. Disc degeneration was confirmed and classified by radiography, magnetic resonance and histological evaluation. Adult male Wistar rats were anesthetized and submitted to percutaneous disc puncture with a 20-gauge needle on levels 6-7 and 8-9 of the coccygeal vertebrae. The needle was inserted into the discs guided by fluoroscopy and its tip was positioned crossing the nucleus pulposus up to the contralateral annulus fibrosus, rotated 360° twice, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intervertebral disc degeneration; Animal models; Histology; Wistar rats.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300235