Consulta: subjectFacets:"Antibiotic resistance"
Registros recuperados: 46
Data/hora: 01/06/2024 08:38:39

Antibacterial activity of the Antarctic bacterium Janthinobacterium sp: SMN 33.6 against multi-resistant Gram-negative bacteria
Provedor de dados: 69
Autores: Asencio,Geraldine; Lavin,Paris; Alegría,Karen; Domínguez,Mariana; Bello,Helia; González-Rocha,Gerardo; González-Aravena,Marcelo.
Background The increment of resistant strains to commonly used antibiotics in clinical practices places in evidence the urgent need to search for new compounds with antibacterial activity. The adaptations that Antarctic microorganisms have developed, due to the extreme environment that they inhabit, promote them as a potential new source of active compounds for the control of microorganisms causing infections associated with health care. The aim of this study was to evaluate the antibacterial activity of an ethanol extract of the Antarctic bacterium Janthinobacterium sp., strain SMN 33.6, against nosocomial multi-resistant Gram-negative bacteria. Results Inhibitory activity against human Gram-negative bacterial pathogens, with concentrations that varied...
Tipo: Journal article
Palavras-chave: Antarctica; Antimicrobial; Antibiotic resistance; Bacterial pigment.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100001

Frequency of transferable multiple antibiotic resistance amongst coliform bacteria isolated from a treated sewage effluent in Antofagasta, Chile
Provedor de dados: 69
Autores: Silva,Juan; Castillo,Gabriela; Callejas,Lorena; López,Héctor; Olmos,Janet.
Antibiotic-resistant coliform bacteria from raw and treated water from activated sludge of a sewage treatment plant were isolated on eosin methylene blue agar (EMB) plates in the separate presence of ampicillin, tetracycline, chloramphenicol and streptomycin. Antibiotic resistant coliforms obtained from inflow and effluent waters were studied to determine influences of treatment on the species composition, resistance patterns and transferable resistance in these bacteria. The bacterial counts obtained showed a high proportion of antibiotic resistant coliforms in the treated effluent. The resistant coliform species were eliminated at different rates by the treatment. Escherichia coli decreased, whereas the proportion of Klebsiella sp increased. The...
Tipo: Journal article
Palavras-chave: Antibiotic resistance; Coliforms; Plasmid; Sewage treatment; Water pollution.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500008

Polymorphism of the rDNA and tDNA loci in clinical isolates of Pseudomonas aeruginosa: a perspective for molecular epidemiology surveillance
Provedor de dados: 74
Autores: Spacov,Isabel Cristina Guerra; Silva,Suzileyde Alberto Marques da; Morais Júnior,Marcos Antônio de; Morais,Márcia Maria Camargo de.
The Gram-negative bacterium Pseudomonas aeruginosa has a wide environmental and ecological distribution. It is an opportunistic pathogen that acquires resistance to multiple antimicrobial agents and can infect plants, animals and humans. We used rDNA and tDNA PCR markers to characterize the bacterial diversity of P. aeruginosa strains isolated at a Brazilian teaching hospital (Oswaldo Cruz University Hospital, Recife, Brazil) between March 2003 and February 2004. Clonal groups of P. aeruginosa clinical isolates were identified from different patients in different hospital units using either rDNA or tDNA markers, or a combination of both in a duplex PCR. These PCR-typing methods together with drug-resistance profiles were used to trace the distribution of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Molecular typing; Nosocomial infection; P. aeruginosa; RDNA-PCR; TDNA-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400023

Environmental and genetic factors affecting mutability to aminoglycoside antibiotics among Escherichia coli K12 strains
Provedor de dados: 74
Autores: Monteiro,A.C.M.; Ferreira,R.C.C; Padilla,G.; Ferreira,L.C.S.; Costa,S.O.P.
Environmental and genetic factors affecting the in vitro spontaneous mutation frequencies to aminoglycoside resistance in Escherichia coli K12 were investigated. Spontaneous mutation frequencies to kanamycin resistance were at least 100 fold higher on modified Luria agar (L2) plates, when compared to results obtained in experiments carried out with Nutrient agar (NA) plates. In contrast to rifampincin, the increased mutability to kanamycin resistance could not be attributed to a mutator phenotype expressed by DNA repair defective strains. Kanamycin mutant selection windows and mutant preventive concentrations on L2 plates were at least fourfold higher than on NA plates, further demonstrating the role of growth medium composition on the mutability to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Aminoglycosides; Escherichia coli K12; Mutator strains; Spontaneous mutantion frequencies.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200017

Bacterial Resistance and the Optimal Use of Antibiotics
Provedor de dados: 31
Autores: Laxminarayan,   Ramanan.
The increasing resistance of harmful biological organisms (bacteria, parasites, and pests) to selection pressure from the widespread use of control agents such as antibiotics, antimalarials, and pesticides is a serious problem in both medicine and agriculture. Modeling resistance-or, conversely, the effectiveness of these control agents as a biological resource-yields insights into how these agents should be optimally managed to maximize their economic benefit to society. This paper uses a model of evolution of bacterial resistance to antibiotics-in which resistance places an evolutionary disadvantage on the resistant organism-to develop a simple sequential algorithm of optimal antibiotic use. Although the solution to this problem follows the...
Tipo: Working or Discussion Paper
Palavras-chave: Antibiotic resistance; Natural resource; Optimization; Health Economics and Policy; I0; Q0.
Ano: 2001
URL: http://purl.umn.edu/10479

An alternative for antibiotic se in poultry: probiotics
Provedor de dados: 89
Autores: Edens,FW.
Over the past 50 years, there has been increasing amounts of antibiotics used prophylactically and as growth promoters. Today, there is a consumer and governmental outcry to eliminate that practice from poultry and livestock production. Evidence has been accumulated to show that there is a link between risk of zoonotic disease and growth promoting antibiotic usage in livestock and poultry. Therefore, alternatives to the use of growth promoting antibiotics must be found to promote growth or production at or near the genetic potential of the modern day broiler, turkey, and egg producer. The use of probiotics has many potential benefits and include modified host metabolism, immuno-stimulation, anti-inflammatory reactions, exclusion and killing of pathogens in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotics; Antibiotic resistance; Benefits; Characteristics; Chickens; Defined culture; Functions; History; Human health risk; Mechanism of action; Probiotics; Turkeys; Undefined culture; Zoonotic disease.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000200001

Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species
Provedor de dados: 63
Autores: Florentino,Ligiane Aparecida; Jaramillo,Paula Marcela Duque; Silva,Karina Barroso; Silva,Jacqueline Savana da; Oliveira,Silvia Maria de; Moreira,Fatima Maria de Souza.
Biological nitrogen fixation, performed by diazotrophic bacteria, plays an important role in the maintenance of agricultural systems, as it contributes with significant amounts of the nitrogen (N) needed for plant growth, totally or partially exempting the use of industrial N fertilisers. Twenty-five bacterial strains isolated from nodules of Leucaena leucocephala and Phaseolus vulgaris trap plants were studied. These nodules were formed after inoculation with suspensions of soil samples collected close to the root system of Sesbania virgata. In previous studies, these bacteria were identified as Cupriavidus necator. This study aimed to evaluate the ability of these strains to fix N2 in the free-living state and to use carbon (C) sources; their resistance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Symbiosis; Β-proteobacteria; Antibiotic resistance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400003

Bovine mastitis in the metropolitan area of Curitiba: antibiotic resistance and antimicrobial control of the infection
Provedor de dados: 52
Autores: Parada,José Luis; Gonçalves,Dicezar; Soccol,Vanete Tomaz; Lima,Marisa; Soccol,Carlos Ricardo.
A study from cows with mastitis was performed and Staphylococcus aureus was the predominant pathogen in 46.4 % among 153 studied strains from 276 milk samples of infected cows. Antibiotic resistance of 71 S. aureus isolates was determined in order to search resistant strains to antibiotics of clinical interest, as well as to determine their degree of multi-resistance. It was found that 60% of the S. aureus strains presented resistance to β-lactams, but none to oxacillin, teicoplamin or vancomycin. On the other hand, with the aim of reducing the use of current antibiotics and their associated resistance, a new formulation was introduced. The antimicrobial compounds (P22-P32), demonstrated to be effective in 55% of the 76 mastitis cases studied. The use of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Staphylococcus aureus; Antibiotic resistance; Infection reduction.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400009

Lactobacilli isolated from Algerian goat's milk as adjunct culture in dairy products
Provedor de dados: 52
Autores: Marroki,Ahmed; Bousmaha-Marroki,Leila.
In this study, nineteen Lactobacillus isolated from Algerian goat's milk, 13 belonging to L. plantarum, three to L. pentosus, two to L. rhamnosus and one to L. fermentum, were examined in vitro in order to be used as adjunct culture in dairy products. The strains were tested for their proteolytic activity, sensory and safety properties. Strains LbMS16 and LbMS21 L. plantarum and LbMF25 L. rhamnosus presented the highest proteolytic activity. All the tested lactobacilli were able to grow on MRS agar containing 0.5 and 1% (W/V) of oxgall, whereas none produced biogenic amine (BA) from the four tested amino acids and were resistant to pH 2.0 and 3.0, but some strains were able to grow at pH 3.5. None of examined strains were β-haemolytic when grown in hors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus; Goat's milk; Safety aspect; Adjunct culture; Sensory properties; Antibiotic resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300015

Overview of tigecycline and its role in the era of antibiotic resistance
Provedor de dados: 55
Autores: Rossi,Flávia; Andreazzi,Denise.
The increasing antimicrobial resistance found in the many clinically important species of bacteria that commonly cause serious and life-threatening diseases presents a difficult challenge for clinicians, especially when an appropriate initial therapy must be chosen. New antibiotics are urgently needed to address the formidable issues associated with infections caused by vancomycin-resistant enterococci (VRE), methicillin-resistant Staphylococcus aureus (MRSA), penicillin-resistant Streptococcus pneumoniae and multidrug-resistant Gram-negative bacteria. The need for new antibiotics that effectively resist antimicrobial mechanisms of resistance has become paramount. Tigecycline is a new antimicrobial agent; it is the first in a new class of antibiotics, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tigecycline; Antibiotic resistance; Antimicrobial mechanisms.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000300010

Vancomycin-resistant van a phenotype Enterococcus faecalis: first case in Minas Gerais state and epidemiological considerations
Provedor de dados: 55
Autores: Ribas,Rosineide Marques; Darini,Ana Lúcia da Costa; Moreira,Tomaz Aquino; Freitas,Claudete; Gontijo Filho,Paulo P.
We report the first case of vancomycin-resistant Enterococcus faecalis with VanA phenotype and vanA genotype isolated from a surgical patient in a general Hospital in Uberlândia, state of Minas Gerais, Brazil.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Nosocomial infections; Antibiotic resistance; VRE.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000400015

Enterobacteriaceae resistant to third generation cephalosporins upon hospital admission: risk factors and clinical outcomes
Provedor de dados: 55
Autores: Oliveira,Mirian Cristina; Oliveira,Clara Rodrigues Alves; Gonçalves,Karine Valéria; Santos,Marciléa Silva; Tardelli,Amanda Cristina Silva; Nobre Jr.,Vandack Alencar.
Objectives: Evaluate risk factors and clinical outcomes of infections caused by Enterobacteriaceae resistant to third-generation cephalosporins present in samples collected upon hospital admission.Methods: Risk factors were evaluated using a 1:2 ratio case-control study. Influence of resistance on the appropriateness of antibiotic therapy, length of stay, and hospital mortality were prospectively evaluated. Characteristics independently associated with the presence of resistant enterobacteria were assessed by logistic regression.Results: Enterobacteria resistant to third-generation cephalosporins were quite common (26.0%). Male gender (OR: 2.66; 95% CI, 1.17-5.06; p= 0.019), invasive prosthesis (OR: 3.79; 95% CI, 1.29-11.08; p= 0.015), previous use of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Third generation cephalosporins; Risk factors; Antibiotic resistance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000300239

Risk factors for pan-resistant Pseudomonas aeruginosa bacteremia and the adequacy of antibiotic therapy
Provedor de dados: 55
Autores: Tuon,Felipe F.; Gortz,Lucas W.; Rocha,Jaime L.
INTRODUCTION: The aim of this study was to determine risk factors for acquiring carbapenemresistant Pseudomonas aeruginosa bacteremia (CR-PA) and factors associated with in-hospital mortality. METHODS: Seventy-seven cases of bacteremia caused by P. aeruginosa were evaluated in a hospital with high incidence of CR-PA. Clinical and laboratorial factors, and previous use of antibiotics were also evaluated. In one analysis, CR-PA and carbapenem-susceptible P. aeruginosa (CS-PA) bacteremia were compared. A second analysis compared patients who died with survivors. RESULTS: Among 77 P. aeruginosa bacteremia, 29 were caused by CR-PA. Admission to the intensive care unit, higher number of total leukocytes, and previous use of carbapenem were statistically...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Imipenem; Meropenem; Antibiotic resistance; Risk factor.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000400007

Increasing prevalence and dissemination of invasive nontyphoidal Salmonella serotype Typhimurium with multidrug resistance in hospitalized patients from southern Brazil
Provedor de dados: 55
Autores: Reis,Rafael Oliveira dos; Souza,Margarida Neves; Cecconi,Maria Cristina Piccoli; Timm,Loeci; Ikuta,Nilo; Simon,Daniel; Wolf,Jonas Michel; Lunge,Vagner Ricardo.
ABSTRACT  Introduction: Nontyphoidal Salmonella serotypes are the main cause of human food-borne infection, including several hospitalization cases in the developing countries.  Aim: To detect the main serotypes and to characterize the antibiotic resistance of human non-enteric and enteric nontyphoidal Salmonella from clinical isolates in Brazil.  Methods: Salmonella serotypes were identified by microbiological and molecular methods. Susceptibility testing to antibiotics was performed by agar disk diffusion. Real-time PCRs were carried out for the detection of the genus Salmonella as well as serotypes Typhimurium and Enteritidis.  Results: A total of 307 nontyphoidal Salmonella were isolated from 289 different patients in a reference laboratory (LACEN-RS)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Antibiotic resistance; Typhimurium; Enteritidis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500424

Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms
Provedor de dados: 55
Autores: Sun,Erlin; Liang,Gehong; Wang,Lining; Wei,Wenjie; Lei,Mingde; Song,Shiduo; Han,Ruifa; Wang,Yubao; Qi,Wei.
Abstract  Aims We sought to characterize the antibiotic susceptibility of strains of Stenotrophomonas maltophilia isolated from clinical samples, and the role of Stenotrophomonas maltophilia biofilm in antibiotic resistance.  Methods Fifty-one clinical Stenotrophomonas maltophilia isolates were obtained from patients with nosocomial infection in the surgical wards and ICUs of six general hospitals in Tianjin, China. In vitro models of Stenotrophomonas maltophilia biofilms were established and confirmed by scanning electron microscopy and fluorescence microscopy with silver staining. The minimal inhibitory concentrations and biofilm inhibitory concentrations of commonly used antibiotics were determined.  Results 47 of 51 strains were resistant to three or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stenotrophomonas maltophilia; Biofilm; Antibiotic resistance; Nosocomial.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400365

Ventilator-associated pneumonia in an adult clinical-surgical intensive care unit of a Brazilian university hospital: incidence, risk factors, etiology, and antibiotic resistance
Provedor de dados: 55
Autores: Rocha,Laura de Andrade da; Vilela,Carolina Assis Pereira; Cezário,Renata Cristina; Almeida,Alair Benedito; Gontijo Filho,Paulo.
The objective of this study was to evaluate clinical characteristics, etiology, and resistance to antimicrobial agents, among patients with ventilator-associated pneumonia (VAP). A case study vs. patients control under mechanical ventilation and hospitalized into clinical-surgical adults ICU of HC-UFU was performed from March/2005 to March/2006. Patients under ventilation for over 48 h were included in the study including 84 with diagnosis of VAP, and 191 without VAP (control group). Laboratory diagnosis was carried out through quantitative microbiological evaluation of tracheal aspirate. The identification of pathogens was performed by classical microbiological tests, and the antibiotics sensitivity spectrum was determined through the CLSI technique. VAP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ventilator-associated pneumonia; Intensive care unit (ICU); Antibiotic resistance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100017

Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem
Provedor de dados: 55
Autores: Freitas,Ana Lúcia Peixoto de; Barth,Afonso Luis.
Susceptibility tests by disk diffusion and by E-test and molecular typing by macrorestriction analysis were performed to determine the relatedness of Pseudomonas aeruginosa isolates from three distinct hospitals. The resistance profile of 124 isolates to 8 antimicrobial agents was determined in three different hospitals, in Porto Alegre, Brazil. Frequencies of susceptibility ranged from 43.9% for carbenicillin to 87.7% for ceftazidime. Cross-resistance data of imipenem-resistant isolates indicated that most (70%) were also resistant to carbenicillin, although 30% remained susceptible to ceftazidime and cefepime. In general, susceptibility profiles were not able to determine relatedness among isolates of P. aeruginosa. On the other hand, molecular typing by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Antibiotic resistance; Imipenem.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000100001

Expression of the marA, soxS, acrB and ramA genes related to the AcrAB/TolC efflux pump in Salmonella entérica strains with and without quinolone resistance-determining regions gyrA gene mutations
Provedor de dados: 55
Autores: Ferrari,Rafaela Gomes; Galiana,Antonio; Cremades,Rosa; Rodríguez,Juan Carlos; Magnani,Marciane; Tognim,Maria Cristina Bronharo; Oliveira,Tereza C.R.M.; Royo,Gloria.
Several studies have been conducted in recent years to elucidate the structure, function and significance of AcrB, MarA, SoxS and RamA in Salmonella enterica. In this study, the relative quantification of acrB, soxS, marA and ramA genes expression was evaluated in 14 strains of S. enterica, with or without accompanying mutations in the quinolone resistance-determining regions of the gyrA gene, that were exposed to ciprofloxacin during the exponential growth phase. The presence of ciprofloxacin during the log phase of bacterial growth activated the genes marA, soxS, ramA and acrB in all S. enterica strains analyzed in this study. The highest expression levels for acrB were observed in strains with gyrA mutation, and marA showed the highest expression in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella sp.; Fluoroquinolone; Efflux pump; Antibiotic resistance.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200002

Clinical and microbiological observational study on AmpC β-lactamase-producing Enterobacteriaceae in a hospital of Nepal
Provedor de dados: 55
Autores: Baral,Pankaj; Neupanea,Sanjiv; Shresthac,Basudha; Ghimirea,Kashi Ram; Marasinia,Bishnu Prasad; Lekhaka,Binod.
Limited information is available regarding AmpC β-lactamase (ABL)-producing Enterobacteriaceae compared to extended-spectrum β-lactamase-producing enterobacteria. Since ABL-producing organisms are often resistant to multiple antimicrobial agents, therapeutic options against these pathogens are limited. Among 230 clinical Enterobacteriaceae isolates, 64 (27.8%) were found to produce ABL in our study. Escherichia coli (83.9%) was a predominant pathogen, followed by Citrobacter freundii (5.2%). A significant proportion of ABL-producing isolates (81.3%) were found to be multidrug resistant against commonly used antibiotics. Univariate analysis showed that prior history of taking antibiotics (odds ratio [OR], 5.278; confidence interval [CI], 2.838-9.817; p &lt;...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: AmpC β-lactamase; Enterobacteriaceae; Antibiotic resistance.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200022

Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV and V isolated from healthy children attending public daycares in northeastern Brazil
Provedor de dados: 55
Autores: Carvalho,Suzi P. de; Almeida,Jéssica B. de; Andrade,Yasmin M.F.S.; Silva,Lucas S.C. da; Oliveira,Arianne C. de; Nascimento,Flávia S.; Campos,Guilherme B.; Oliveira,Márcio V.; Timenetsky,Jorge; Marques,Lucas M.
Abstract Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) have increasingly been reported in healthy communities. This study aimed to assess the rate of S. aureus in general and MRSA in particular from nasal secretion of children in daycare centers in Vitória da Conquista, Brazil. The isolates were identified based on morphology, biochemical tests and by PCR. Detection of virulence genes, biofilm production, and susceptibility test by disk diffusion agar were performed. MRSA isolates were characterized by spa, SCCmec, and multilocus sequence typing (MLST). S. aureus were recovered from 70 (47.3%) of 148 children. Among the 11 MRSA strains (15.7%), two SCCmec types (IV and V) were detected. MLST identified four STs related to three...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CA-MRSA; Antibiotic resistance; SCCmec; MLST.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400464

Factors associated with bacteremia due to multidrug-resistant Gram-negative bacilli in hematopoietic stem cell transplant recipients
Provedor de dados: 56
Autores: Garnica,M.; Maiolino,A.; Nucci,M.
The epidemiology of bacteremia developing during neutropenia has changed in the past decade, with the re-emergence of Gram-negative (GN) bacteria and the development of multidrug resistance (MDR) among GN bacteria. We conducted a case-control study in order to identify factors associated with bacteremia due to multidrug-resistant Gram-negative (MDRGN) isolates in hematopoietic stem cell transplant recipients. Ten patients with MDRGN bacteremia were compared with 44 patients with GN bacteremia without MDR. Bacteremia due to Burkholderia or Stenotrophomonas sp was excluded from analysis (3 cases), because the possibility of intrinsical resistance. Infection due to MDRGN bacteria occurred in 2.9% of 342 hematopoietic stem cell transplant recipients....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hematopoietic stem cell transplant; Neutropenia; Gram-negative bacteria; Antibiotic resistance; Risk factors.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300010

BCG lymphadenopathy detected in a BCG-vaccinated infant
Provedor de dados: 56
Autores: Barouni,A.S.; Augusto,C.; Queiroz,M.V.N.P.; Lopes,M.T.P.; Zanini,M.S.; Salas,C.E.
Large-scale vaccination with BCG, the live attenuated strain of Mycobacterium bovis, is being adopted around the world, although sporadic complications have occurred after the procedure. Lymphadenopathy is not uncommon especially in babies under one year (0.73% of vaccinated infants), but the swelling subsides within 2 months in most cases, with no medical or surgical treatment. Brazil adopted BCG vaccination program earlier in the seventies and by 1995 more than 96% of the infant population received this immunization. We report here the occurrence of lymphadenopathy in a two-year-old child vaccinated with the Brazilian BCG strain. The diagnosis was made using a lymph node biopsy and intestinal aspirates that yielded a positive mycobacterial culture. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bacille Calmette-Guérin (BCG); Vaccination; Polymerase chain reaction; Lymphadenopathy; Antibiotic resistance.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500011

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil
Provedor de dados: 58
Autores: Rodolpho,Daniela; Marin,José Moacir.
Shiga toxigenic Escherichia coli (STEC) has been implicated as the cause of several human diseases. Samples (ground beef, grinding-machines and human hands) from 23 butcheries were assayed for E. coli using standard microbiological methods, and 287 isolates were submitted to polymerase chain reaction for the detection of stx 1, stx 2 and eae genes. Four STEC isolates were recovered, two from ground beef and two from grinding-machines; all harbored the stx 2 gene and were negative for the eae gene. All E. coli isolates including the four STEC were screened for antibiotic resistance. High levels of resistance against several antimicrobial agents were detected; those most commonly observed were to tetracycline (76.6%), amoxicillin (64.1%) and cephalothin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; STEC; Stx 2; Antibiotic resistance; Ground beef; Cattle.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400004

Antibiotic resistance in Salmonella Enteritidis isolated from broiler carcasses
Provedor de dados: 58
Autores: Cardoso,Martha Oliveira; Ribeiro,Aldemir Reginato; Santos,Luciana Ruschel dos; Pilotto,Fernando; Moraes,Hamilton L.S. de; Salle,Carlos Tadeu Pippi; Rocha,Silvio Luís da Silveira; Nascimento,Vladimir Pinheiro do.
Eighty Salmonella Enteritidis strains isolated from broiler carcasses between May 1995 and April 1996 in the State of Rio Grande do Sul, Brazil, were tested for antibiotic susceptibility using the disk diffusion method. Resistance to colistin, novobiocin, erythromycin and tetracycline was observed in 100% of the isolates. The strains showed intermediate resistance at different levels to kanamycin (1.25%), enrofloxacin (3.75%), neomycin (3.75%), fosfomycin (20%), sulphonamides (86.25%) and nitrofurantoin (90%). Resistance to ciprofloxacin, norfloxacin, gentamicin, polymyxin B, sulphametrim and sulphazotrim was not found. Since resistance to antibiotics especially those introduced in the last decades, was detected, it is recommended that their use must be...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antibiotic resistance; Broiler carcasses; Salmonella Enteritidis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300030

Isolation of Dickeya dadantii strains from potato disease and biocontrol by their bacteriophages
Provedor de dados: 58
Autores: Soleimani-Delfan,Abbas; Etemadifar,Zahra; Emtiazi,Giti; Bouzari,Majid.
One of the most economically important bacterial pathogens of plants and plant products is Dickeya dadantii. This bacterium causes soft rot disease in tubers and other parts of the potato and other plants of the Solanaceae family. The application of restricted host range bacteriophages as biocontrol agents has recently gained widespread interest. This study purposed to isolate the infectious agent of the potato and evaluate its biocontrol by bacteriophages. Two phytopathogenic strains were isolated from infected potatoes, identified based on biochemical and 16S rRNA gene sequencing, and submitted to GenBank as D. dadantii strain pis3 (accession no. HQ423668) and D. dadantii strain sip4 (accession no. HQ423669). Their bacteriophages were isolated from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dickeya dadantii; Soft rot; Bacteriophage; Antibiotic resistance; Biocontrol.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300791

Primary antibiotic resistance and its relationship with cagA and vacA genes in Helicobacter pylori isolates from Algerian patients
Provedor de dados: 58
Autores: Bachir,Meryem; Allem,Rachida; Tifrit,Abedelkarim; Medjekane,Meriem; Drici,Amine El-Mokhtar; Diaf,Mustafa; Douidi,Kara Turki.
Abstract The epidemiology of Helicobacter pylori resistance to antibiotics is poorly documented in Africa and especially in Algeria. The aim of our study was to determine the antibiotic resistance rates, as well as its possible relationship with VacA and CagA virulence markers of isolates from Algerian patients. One hundred and fifty one H. pylori isolate were obtained between 2012 and 2015 from 200 patients with upper abdominal pain. Antimicrobial susceptibility testing was performed for amoxicillin, clarithromycin, metronidazole, ciprofloxacin, rifampicin and tetracycline. Molecular identification of H. pylori and the detection of vacA and cagA genes were performed using specific primers. We found that H. pylori was present in 83.5% of collected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Antibiotic resistance; Vaca; Caga; Algeria.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300544

Safety, beneficial and technological properties of Enterococcus faecium isolated from Brazilian cheeses
Provedor de dados: 58
Autores: Santos,Karina Maria Olbrich dos; Vieira,Antônio Diogo Silva; Salles,Hévila Oliveira; Oliveira,Jacqueline da Silva; Rocha,Cíntia Renata Costa; Borges,Maria de Fátima; Bruno,Laura Maria; Franco,Bernadette Dora Gombossy de Melo; Todorov,Svetoslav Dimitrov.
This study aimed to characterize the safety and technological properties of Enterococcus faecium strains isolated from Brazilian Coalho cheeses. High levels of co-aggregation were observed between Enterococcus faecium strains EM485 and EM925 and both Escherichia coli and Clostridium perfringens. Both strains presented low levels of hydrophobicity. E. faecium EM485 and EM925 were both able to grow in the presence of 0.5% of the sodium salts of taurocholic acid (TC), taurodeoxycholic acid (TDC), glycocholic acid (GC), and glycodeoxycholic acid (GDC), although they showed the ability to deconjugate only GDC and TDC. Both strains showed good survival when exposed to conditions simulating the gastro intestinal tract (GIT). When tested for the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterococcus faecium; Probiotic properties; Technological properties; Virulence factors; Antibiotic resistance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100237

Technology and safety assessment for lactic acid bacteria isolated from traditional Bulgarian fermented meat product "lukanka"
Provedor de dados: 58
Autores: Todorov,Svetoslav Dimitrov; Stojanovski,Saso; Iliev,Ilia; Moncheva,Penka; Nero,Luis Augusto; Ivanova,Iskra Vitanova.
Abstract The present work discusses the technological and new selection criteria that should be included for selecting lactic acid bacteria for production of fermented meat. Lactic acid bacteria isolated from Bulgarian traditional fermented "lulanka" salami was studied regarding some positive technological parameters (growth at different temperature, pH, and proteolytic activity). The presence of genes related to the virulence factors, production of biogenic amines, and vancomycin resistance were presented in low frequency in the studied lactic acid bacteria. On the other hand, production of antimicrobial peptides and high spread of bacteriocin genes were broadly presented. Very strong activity against L. monocytogenes was detected in some of the studied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lukanka; Antibiotic resistance; Virulence factors; Lactic acid bacteria; Bacteriocins.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300576

pMEX01, a 70 kb plasmid isolated from Escherichia coli that confers resistance to multiple β-lactam antibiotics
Provedor de dados: 58
Autores: Hernandez-Flores,Jose Luis; Pérez,Juan Caballero; Gutiérrez,Carlos Saldaña; Hernández,Andrés Cruz; Alonso,Gerardo Soto; Hernández,Sergio Pacheco; Gómez,Sergio Romero; Fernández,Francisco; Loske,Achim M.; Guillén,Juan Campos.
Abstract Multidrug-resistant microorganisms are of great concern to public health. Genetic mobile elements, such as plasmids, are among the most relevant mechanisms by which bacteria achieve this resistance. We obtained an Escherichia coli strain CM6, isolated from cattle presenting severe diarrheic symptoms in the State of Querétaro, Mexico. It was found to contain a 70 kb plasmid (pMEX01) with a high similarity to the pHK01-like plasmids that were previously identified and described in Hong Kong. Analysis of the pMEX01 sequence revealed the presence of a blaCTX-M-14 gene, which is responsible for conferring resistance to multiple β-lactam antibiotics. Several genes putatively involved in the conjugative transfer were also identified on the plasmid. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Plasmid; Antibiotic resistance; BlaCTX-M-14 gene.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300569

Phenotypic and genotypic diversity of Pseudomonas aeruginosa strains isolated from hospitals in siedlce (Poland)
Provedor de dados: 58
Autores: Wolska,Katarzyna; Kot,Barbara; Jakubczak,Antoni.
A total of 62 Pseudomonas aeruginosa strains isolated from two hospitals in Siedlce (Poland) were studied by repetitive element based PCR (rep-PCR) using BOX primer. BOX-PCR results revealed the presence of 7 numerous genotypes and 31 unique patterns among isolates. Generally, the strains of P. aeruginosa were characterized by resistance to many antibiotics tested and by differences in serogroups and types of growth on cetrimide agar medium. However, the P. aeruginosa strains isolated from faeces showed much lower phenotypic and genotypic variations in comparison with strains obtained from other clinical specimens. It was observed that genetic techniques supported by phenotypic tests have enabled to conduct a detailed characterization of P. aeruginosa...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; BOX-PCR; Antibiotic resistance; Serotyping; Cetrimide agar.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100032

Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater
Provedor de dados: 58
Autores: Alam,Mohammad Zubair; Aqil,Farrukh; Ahmad,Iqbal; Ahmad,Shamim.
This study reports the occurrence of antibiotic resistance and production of β-lactamases including extended spectrum beta-lactamases (ESβL) in enteric bacteria isolated from hospital wastewater. Among sixty-nine isolates, tested for antibiotic sensitivity, 73.9% strains were resistant to ampicillin followed by nalidixic acid (72.5%), penicillin (63.8%), co-trimoxazole (55.1%), norfloxacin (53.6%), methicillin (52.7%), cefuroxime (39.1%), cefotaxime (23.2%) and cefixime (20.3%). Resistance to streptomycin, chloramphenicol, nitrofurantoin, tetracycline, and doxycycline was recorded in less than 13% of the strains. The minimum inhibitory concentration (MIC) showed a high level of resistance (800-1600 µg/mL) to one or more antibiotics. Sixty three (91%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta-lactamase; ESβL; Antibiotic resistance; Enteric bacteria; Plasmid transfer.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300021

Characterization of the variable region in the class 1 integron of antimicrobial-resistant Escherichia coli isolated from surface water
Provedor de dados: 58
Autores: Canal,Natália; Meneghetti,Karine Lena; Almeida,Clara Ponzi de; Bastos,Marina da Rosa; Otton,Letícia Muner; Corção,Gertrudes.
Abstract Fecal bacteria are considered to be a potential reservoir of antimicrobial resistance genes in the aquatic environment and could horizontally transfer these genes to autochthonous bacteria when carried on transferable and/or mobile genetic elements. Such circulation of resistance genes constitutes a latent public health hazard. The aim of this study was to characterize the variable region of the class 1 integron and relate its genetic content to resistance patterns observed in antimicrobial-resistant Escherichia coli isolated from the surface waters of Patos Lagoon, Southern Brazil. Genetic diversity of the isolates and presence of the qacEΔ1 gene, which confers resistance to quaternary ammonium compounds, were also investigated. A total of 27...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Class 1 integron; Superficial water; Escherichia coli.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200337

Prevalence, serotyping and antimicrobials resistance mechanism of Salmonella enterica isolated from clinical and environmental samples in Saudi Arabia
Provedor de dados: 58
Autores: El-Tayeb,Mohamed A.; Ibrahim,Abdelnasser S.S.; Al-Salamah,Ali A.; Almaary,Khalid S.; Elbadawi,Yahya B.
Abstract Salmonella is recognized as a common foodborne pathogen, causing major health problems in Saudi Arabia. Herein, we report epidemiology, antimicrobial susceptibility and the genetic basis of resistance among S. enterica strains isolated in Saudi Arabia. Isolation of Salmonella spp. from clinical and environmental samples resulted in isolation of 33 strains identified as S. enterica based on their biochemical characteristics and 16S-rDNA sequences. S. enterica serovar Enteritidis showed highest prevalence (39.4%), followed by S. Paratyphi (21.2%), S. Typhimurium (15.2%), S. Typhi and S. Arizona (12.1%), respectively. Most isolates were resistant to 1st and 2nd generation cephalosporin; and aminoglycosides. Moreover, several S. enterica isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Serotyping; Antibiotic resistance; Antimicrobials resistance determinants.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300499

Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil
Provedor de dados: 58
Autores: Lemes-Marques,Eneida Gonçalves; Cruz,Cristina Durante; Destro,Maria Teresa.
Considering the lack of information available in Brazil concerning listeriosis, the present study aimed to analyze phenotypic and genotypically Listeria monocytogenes clinical isolates from the Southwestern region of the State of São Paulo, Brazil. From January 1995 to May 2005, thirteen isolates of L. monocytogenes from twelve patients with listeriosis were sent to the Adolfo Lutz Institute (Campinas Regional Laboratory, São Paulo, Brazil). The antimicrobial susceptibility of the isolates was evaluated by the microdilution broth method for ampicillin, gentamicin, trimethoprim, sulfamethoxazole and vancomycin. They were also serotyped (Denka Seiken, Japan), and sub-typed by PFGE employing ApaI and AscI and the American CDC protocol. None of the isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Serotypes; Antibiotic resistance; Genotypic characterization; Listeriosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200019

Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
Provedor de dados: 58
Autores: Martins,Katheryne Benini; Faccioli-Martins,Patricia Yoshida; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli; Fernandes,Simone; Oliveira,Aline A.; Dantas,Ariane; Zafalon,Luiz Francisco; Cunha,Maria de Lourdes Ribeiro de Souza da.
The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Sheep; Staphylococcus aureus; Antibiotic resistance; Virulence factors.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535

Complete genome sequence of human pathogen Kosakonia cowanii type strain 888-76T
Provedor de dados: 58
Autores: Yang,Xue-Jing; Wang,Sai; Cao,Jun-Min; Hou,Jia-Hui.
ABSTRACT Kosakonia cowanii type strain 888-76T is a human pathogen which was originally isolated from blood as NIH group 42. In this study, we report the complete genome sequence of K. cowanii 888-76T. 888-76T has 1 chromosome and 2 plasmids with a total genome size of 4,857,567 bp and C+G 56.15%. This genome sequence will not only help us to understand the virulence features of K. cowanii 888-76T but also provide us the useful information for the study of evolution of Kosakonia genus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kosakonia cowanii; Genome sequence; Antibiotic resistance.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100016

Multiresistance and endemic status of acinetobacter baumannii associated with nosocomial infections in a tunisian hospital: a critical situation in the intensive care units
Provedor de dados: 58
Autores: Ben Othman,A.; Zribi,M.; Masmoudi,A.; Abdellatif,S.; Ben Lakhal,S.; Fendri,C.
Acinetobacter baumannii is often implicated in hospital outbreaks in Tunisia. It's a significant opportunistic pathogen associated with serious underlying diseases such as pneumoniae, meningitis and urinary tract infections. The aim of our study was to evaluate its degree of endemicity and its antibiotic resistance evolution essentially in the unit care where its isolation was predominant (57%). This study used 3 methods: antibiotyping, RAPD using 2 primers VIL 1, VIL5 and PFGE with ApaI restriction enzyme. The presence of integron1 and 2 was also studied. Antibiotyping showed that 92% of patients were resistant of all ß- lactams (except Imipenem) and that the resistance to Imipenem occurred in 47% of cases. RAPD profiles obtained with the 2 arbitrarily...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: A. baumannii; Epidemiology; Antibiotic resistance; RAPD; PFGE.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200001

Antibiotic susceptibility profile of bacilli isolated from the skin of healthy humans
Provedor de dados: 58
Autores: Tarale,Prashant; Gawande,Sonali; Jambhulkar,Vinay.
Abstract In the present work, twelve bacilli were isolated from four different regions of human skin from Bela population of Nagpur district, India. The isolated bacilli were identified by their morphological, cultural and biochemical characteristics. Seven isolates were Gram negative rods, out of which five were belong to genus Pseudomonas. Three among the five Gram positive isolates were identified as Dermabactor and the remaining two Bacillus. Their antimicrobial susceptibility profile was determined by Kirby-Bauer disc diffusion method. The isolates showed resistance to several currently used broad-spectrum antibiotics. The Dermabactor genus was resistant to vancomycin, although it was earlier reported to be susceptible. Imipenem was found to be the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Identification; Microflora; Nosocomial; Opportunistic.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401111

Multiple drug resistant Staphylococcus aureus strains isolated from a fish market and from fish handlers
Provedor de dados: 58
Autores: Albuquerque,W.F.; Macrae,A.; Sousa,O.V.; Vieira,G.H.F.; Vieira,R.H.S.F.
The aim of this study was to investigate the presence of antibiotic resistant Staphylococcus aureus strains in fish stalls and in hands and nasal and oral cavities of fish handlers of the Mucuripe Fish Market, Fortaleza, Ceará, Brazil. All S. aureus isolates were resistant to Ampicillin and 44 % were multi-drug resistant.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish products; Fish handlers; Antibiotic resistance; S. aureus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100027

Study of pandrug and heavy metal resistance among E. coli from anthropogenically influenced Delhi stretch of river Yamuna
Provedor de dados: 58
Autores: Azam,Mudsser; Jan,Arif Tasleem; Kumar,Ashutosh; Siddiqui,Kehkashan; Mondal,Aftab Hossain; Haq,Qazi Mohd. Rizwanul.
Abstract Escalating burden of antibiotic resistance that has reached new heights present a grave concern to mankind. As the problem is no longer confined to clinics, we hereby report identification of a pandrug resistant Escherichia coli isolate from heavily polluted Delhi stretch of river Yamuna, India. E. coli MRC11 was found sensitive only to tobramycin against 21 antibiotics tested, with minimum inhibitory concentration values &gt;256 µg/mL for amoxicillin, carbenicillin, aztreonam, ceftazidime and cefotaxime. Addition of certain heavy metals at higher concentrations were ineffective in increasing susceptibility of E. coli MRC11 to antibiotics. Withstanding sub-optimal concentration of cefotaxime (10 µg/mL) and mercuric chloride (2 µg/mL), and also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; ESBLs; Heavy metal resistance; Polluted environment; Resistance genes.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300471

Bacteriocins produced by L. fermentum and L .acidophilus can inhibit cephalosporin resistant E .coli.
Provedor de dados: 58
Autores: Riaz,Saba; Nawaz,Syed Kashif; Hasnain,Shahida.
Reemerging infections occur due to resistant bacteria. Such infections create restrictions for clinicians and microbiologists in drug selection. Such problems demand new strategies for solution. Use of bacteriocins for this purpose may be fruitful. In the present research work, the inhibitory effects of bactericins on cephalosporin resistant Escherichia coli are used as model system for the control of antibiotic resistant pathogenic bacteria. Cephalosporin resistant Escherichia coli strain was isolated from pus by using conventional methodology. For bacteriocin production, Lactobacilli strains were selected by using selective media. Out of seventy two strains isolated from yogurt, fecal materials of human, chick, parrot and cat, only two strains (strain 45...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cephalosporin resistant Escherichia coli; Bacteriocin; Antibiotic resistance; Lactobacillus fermentum; Lactobacillus acidophilus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300015

Pathogenic factors and antimicrobial resistance of Staphylococcus epidermidis associated with nosocomial infections occurring in intensive care units
Provedor de dados: 58
Autores: Michelim,Lessandra; Lahude,Michele; Araújo,Patricia R.; Giovanaz,Dirce S. H.; Müller,Gabriela; Delamare,Ana P.L.; Costa,Sérgio Olavo Pinto da; Echeverrigaray,Sérgio.
Nosocomial infections constitute an important problem in hospitals, intensive care units (ICU) having the highest incidence of this type of infection. Staphylococci, especially Staphylococcus epidermidis and Staphylococcus aureus, are among the most important microorganisms associated with nosocomial infections. S. epidermidis is a common skin resident, and can be introduced into the clinical environment by patients and hospital staff. The situation in hospitals is aggravated by the emergence of multiresistant strains. We evaluated 98 hospital S. epidermidis isolates collected at neonatal, pediatric and adult ICUs and 20 S. epidermidis control skin resident isolates from healthy volunteers, for resistance to ten antibiotics and chemotherapeutic agents, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus epidermidis; Pathogenicity factors; Antibiotic resistance; Hospital infection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100004

Bactericidal antibiotic-phytochemical combinations against methicillin resistant Staphylococcus aureus
Provedor de dados: 58
Autores: Kyaw,Bhone Myint; arora,Shuchi; Lim,Chu Sing.
Methicillin resistant Staphylococcus aureus (MRSA) infection is a global concern nowadays. Due to its multi-drug resistant nature, treatment with conventional antibiotics does not assure desired clinical outcomes. Therefore, there is a need to find new compounds and/or alternative methods to get arsenal against the pathogen. Combination therapies using conventional antibiotics and phytochemicals fulfill both requirements. In this study, the efficacy of different phytochemicals in combination with selected antibiotics was tested against 12 strains of S. aureus (ATCC MRSA 43300, ATCC methicillin sensitive S. aureus or MSSA 29213 and 10 MRSA clinical strains collected from National University Hospital, Singapore). Out of the six phytochemicals used, tannic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phytochemicals; Antibiotic combinations; Synergism; Antibiotic resistance; Methicillin-resistance Staphylococcus aureus (MRSA).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300013

Molecular characterization of vanA-containing Enterococcus from migratory birds: song thrush (Turdus philomelos)
Provedor de dados: 58
Autores: Silva,Nuno; Igrejas,Gilberto; Felgar,Ana; Gonçalves,Alexandre; Pacheco,Rui; Poeta,Patrícia.
Vancomycin-resistant enterococci (VRE) were detected in two faecal samples (1.3%) of song thrush in Portugal. vanA isolates showed high level vancomycin/teicoplanin resistance, as well as resistance to ciprofloxacin, quinupristin-dalfopristin and cloranfenicol. Thrush can be a reservoir of VRE and transmit these resistant bacteria to other animals including humans.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Migratory birds; VanA-containing enterococci; Antibiotic resistance; Portugal.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300026

Frequency of zoonotic bacteria among illegally traded wild birds in Rio de Janeiro
Provedor de dados: 58
Autores: Matias,Carlos Alexandre Rey; Pereira,Ingrid Annes; Reis,Eliane Moura Falavina dos; Rodrigues,Dália dos Prazeres; Siciliano,Salvatore.
Abstract The illegal wildlife trade may increase the risk of infectious disease transmission, and it may not only cause disease outbreaks in humans but also threaten livestock, native wild populations, and ecosystems' health. Bird species may act as carriers in the transmission of enteric pathogens. However, epidemiological studies on zoonotic bacteria in wild birds are rare in Brazil. From March 2011 to March 2012, we investigated the frequency of Enterobacteriaceae in cloacal swab samples from 109 birds of the passerine and Psittacidae families. These birds were recovered from illegal trade in Rio de Janeiro, Brazil, and sent to a rehabilitation center. Gram-negative bacteria were isolated from 86 wild birds (78.9%). A mean (±SD) of 1.68 (±1.30)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Enterobacteriaceae; Public health; Salmonella; Wild birds.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400882

Positive, negative and marker-free strategies for transgenic plant selection
Provedor de dados: 61
Autores: Aragão,Francisco José Lima; Brasileiro,Ana Cristina Miranda.
In this review, the use of the most common selection marker genes on plant transformation and the effects of their respective selective agents are discussed. These genes could be divided in two categories according their mode of action: genes for positive and negative selection. The retention of the marker gene flow through chloroplast transformation is also discussed. Further, strategies to recover marker-free transgenic plants, involving multi-auto-transformation (MAT), co-transformation, site-specific recombination and intragenomic relocation of transgenes through transposable elements are reviewed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Herbicide resistance; Marker genes; Plant transformation; Selection; Transgenic plant.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000100001