Consulta: subjectFacets:"Antigenotoxicity"
Registros recuperados: 13
Data/hora: 07/06/2024 09:49:02

Chemical composition and antigenotoxic properties of Lippia alba essential oils
Provedor de dados: 74
Autores: López,Molkary Andrea; Stashenko,Elena E.; Fuentes,Jorge Luis.
The present work evaluated the chemical composition and the DNA protective effect of the essential oils (EOs) from Lippia alba against bleomycin-induced genotoxicity. EO constituents were determined by Gas Chromatography/Mass Spectrometric (GC-MS) analysis. The major compounds encountered being citral (33% geranial and 25% neral), geraniol (7%) and trans-β-caryophyllene (7%) for L. alba specimen COL512077, and carvone (38%), limonene (33%) and bicyclosesquiphellandrene (8%) for the other, COL512078. The genotoxicity and antigenotoxicity of EO and the compounds citral, carvone and limonene, were assayed using the SOS Chromotest in Escherichia coli. The EOs were not genotoxic in the SOS chromotest, but one of the major compound (limonene) showed genotoxicity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lippia alba; Essential oil; Antigenotoxicity; Bleomycin; SOS chromotest.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300019

The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest
Provedor de dados: 74
Autores: Quintero,Nathalia; Stashenko,Elena E.; Fuentes,Jorge Luis.
In this work, the toxicity and genotoxicity of organic solvents (acetone, carbon tetrachloride, dichloromethane, dimethylsulfoxide, ethanol, ether and methanol) were studied using the SOS chromotest. The influence of these solvents on the direct genotoxicity induced by the mutagens mitomycin C (MMC) and 4-nitroquinoline-1-oxide (4-NQO) were also investigated. None of the solvents were genotoxic in Escherichia coli PQ37. However, based on the inhibition of protein synthesis assessed by constitutive alkaline phosphatase activity, some solvents (carbon tetrachloride, dimethylsulfoxide, ethanol and ether) were toxic and incompatible with the SOS chromotest. Solvents that were neither toxic nor genotoxic to E. coli (acetone, dichloromethane and methanol)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxicity; Genotoxicity; Interference; Mitomycin C; 4-nitro-quinoline-1-oxide; SOS chromotest; Solvents; Vitamin E.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300018

Evaluation of the genotoxic and antigenotoxic potential of Melissa officinalis in mice
Provedor de dados: 74
Autores: Carvalho,Natália Cassettari de; Corrêa-Angeloni,Maria Júlia Frydberg; Leffa,Daniela Dimer; Moreira,Jeverson; Nicolau,Vanessa; Amaral,Patrícia de Aguiar; Rossatto,Ângela Erna; Andrade,Vanessa Moraes de.
Melissa officinalis (L.) (Lamiaceae), a plant known as the lemon balm, is native to the east Mediterranean region and west Asia. Also found in tropical countries, such as Brazil, where it is popularly known as "erva-cidreira" or "melissa", it is widely used in aqueous- or alcoholic-extract form in the treatment of various disorders. The aim was to investigate in vivo its antigenotoxicity and antimutagenicity, as well as its genotoxic/mutagenic potential through comet and micronucleus assaying. CF-1 male mice were treated with ethanolic (Mo-EE) (250 or 500 mg/kg) or aqueous (Mo-AE) (100 mg/kg) solutions of an M. officinalis extract for 2 weeks, prior to treatment with saline or Methyl methanesulfonate (MMS) doses by intraperitoneal injection. Irrespective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melissa officinalis; Comet assay; Micronucleus test; Genotoxicity; Antigenotoxicity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200021

In vivo evaluation of the antimutagenic and antigenotoxic effects of β-glucan extracted from Saccharomyces cerevisiae in acute treatment with multiple doses
Provedor de dados: 74
Autores: Oliveira,Rodrigo Juliano; Salles,Maria José Sparça; Silva,Ariane Fernanda da; Kanno,Tatiane Yumi Nakamura; Lourenço,Ana Carolina dos Santos; Leite,Véssia da Silva; Matiazi,Hevenilton José; Pesarini,João Renato; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
Ample evidence suggests that cancer is triggered by mutagenic damage and diets or supplements capable of reducing such incidences can be related to the prevention of neoplasy development or to an improvement in life quality of patients who undergo chemotherapy. This research aimed to evaluate the antimutagenic and antigenotoxic activity of β-glucan. We set up 8 experimental groups: control (Group 1), cyclophosphamide (Group 2), Groups 3-5 to assess the effect of β-glucan administration, and Groups 6-8 to evaluate the association between cyclophosphamide and β-glucan. The intraperitonial concentrations of β-glucan used were 100, 150 and 200 mg/kg. Micronucleus and comet assays showed that within the first week of treatment β-glucan presented a damage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-glucan; Cyclophosphamide; Antimutagenicity; Antigenotoxicity; Mice.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300017

Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assay
Provedor de dados: 74
Autores: Miyaji,CK; Jordão,BQ; Ribeiro,LR; Eira,AF; Cólus,IMS.
The mushroom shiitake (Lentinula edodes (Berkeley) Pegler) is been widely consumed in many countries, including Brazil, because of its pleasant flavor and reports of its therapeutic properties, although there is little available information on the genotoxicity and/or antigenotoxicity of this mushroom. We used the Comet assay and HEp-2 cells to evaluate the in vitro genotoxic and antigenotoxic activity of aqueous extracts of shiitake prepared in three different concentrations (0.5, 1.0 and 1.5 mg/mL) and three different temperatures (4, 22 and 60 °C), using methyl methanesulfonate (MMS) as a positive control and untreated cells as a negative control. Two concentrations (1.0 and 1.5 mg/mL) of extract prepared at 4 °C and all of the concentrations prepared at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shiitake; Comet assay; HEp-2 cells; Genotoxicity; Antigenotoxicity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100018

Protection by Panax ginseng C.A. Meyer against the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster
Provedor de dados: 74
Autores: Pereira,Denise G.; Antunes,Lusânia M.G.; Graf,Ulrich; Spanó,Mário A.
Panax ginseng is one of the most widely prescribed herbal medicines for the treatment of cancer, diabetes, chronic inflammation, and neurodegenerative and cardiovascular diseases. Since the use of alternative medicines in combination with conventional therapy may increase the risk of unwanted interactions, we investigated the possible genotoxicity of a water-soluble form of the dry root of P. ginseng (2.5, 5.0 or 10.0 mg/mL) and its ability to protect against the genotoxicity of doxorubicin (DOX; 0.125 mg/mL) by using the Drosophila melanogaster wing somatic mutation and recombination test (SMART) with standard and high-bioactivation crosses of flies. Panax ginseng was not genotoxic at the concentrations tested, whereas DOX-induced genotoxicity in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxicity; SMART; Wing spot test.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500024

Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects
Provedor de dados: 74
Autores: Mendonça,Leonardo Meneghin; Machado,Carla da Silva; Teixeira,Cristiane Cardoso Correia; Freitas,Luis Alexandre Pedro de; Bianchi,Maria Lourdes Pires; Antunes,Lusânia Maria Greggi.
Abstract Curcumin (CMN) is the principal active component derived from the rhizome of Curcuma longa (Curcuma longa L.). It is a liposoluble polyphenolic compound that possesses great therapeutic potential. Its clinical application is, however, limited by the low concentrations detected following oral administration. One key strategy for improving the solubility and bioavailability of poorly water-soluble drugs is solid dispersion, though it is not known whether this technique might influence the pharmacological effects of CMN. Thus, in this study, we aimed to evaluate the antioxidant and antigenotoxic effects of CMN formulated in a solid dispersion (CMN SD) compared to unmodified CMN delivered to Wistar rats. Cisplatin (cDDP) was used as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcuma longa; Antigenotoxicity; Micronucleus test; DNA damage; Comet assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400490

Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays
Provedor de dados: 74
Autores: Leite-Silva,Cleide; Gusmão,Cássia Lima Silva; Takahashi,Catarina Satie.
The brown seaweed Fucus vesiculosus (Fucales, Fucaceae) was screened for its protective activity using doxorubicin-induced DNA damage in human lymphocytes. In this study, we assessed the genotoxic and antigenotoxic potential of three different concentrations (0.25, 0.5 and 1.0 mg mL-1) of F. vesiculosus aqueous extract using the chromosome aberration and Comet assays. Treatment of human lymphocyte cultures with 0.25, 0.5 and 1.0 mg mL-1 F. vesiculosus aqueous extract had no effect on the chromosome aberration frequency or on the extent of DNA damage detected by the Comet assay. The antigenotoxic effects of the extract were tested in human lymphocyte cultures treated with 15 µg mL-1 of doxorubicin, either alone or combined with the different concentrations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxicity; Chromosomal aberrations; Comet assay; Doxorubicin; Fucus vesiculosus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100019

Antigenotoxic effects of Mandevilla velutina (Gentianales, Apocynaceae) crude extract on cyclophosphamide-induced micronuclei in Swiss mice and urethane-induced somatic mutation and recombination in Drosophila melanogaster
Provedor de dados: 74
Autores: Silva,Regildo Márcio Gonçalves da; Sousa,Neila Coelho de; Graf,Ulrich; Spanó,Mário Antônio.
A Mandevilla velutina crude extract was investigated using the mouse micronucleus test (MNT) and the Drosophila melanogaster somatic mutation and recombination test (SMART) using standard (ST) and high bioactivation (HB) crosses. The MNT used 10 mg, 20 mg or 40 mg per 100 g of body weight (bw) of extract with and without 0.2 mg per 100 g bw peritoneal cyclophosphamide. There was no genotoxicity in the negative control or extract only groups and, compared to the cyclophosphamide control, there was a significant reduction in micronucleated polychromatic erythrocytes in all the groups given extract plus cyclophosphamide. For SMART larvae were fed 5 or 10 mg mL-1 of extract for seven days with and without 0.89 mg mL-1 of urethane given on day seven. The ST and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxicity; Genotoxicity tests; Micronucleus assay; SMART; Wing spot test.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400023

Antimutagenic and antirecombinagenic activities of noni fruit juice in somatic cells of Drosophila melanogaster
Provedor de dados: 42
Autores: FRANCHI,LEONARDO P.; GUIMARAES,NILZA N.; ANDRADE,LAISE R. DE; ANDRADE,HELOISA H.R. DE; LEHMANN,MAURICIO; DIHL,RAFAEL R.; CUNHA,KENYA S.
Noni, a Hawaiian name for the fruit of Morinda citrifolia L., is a traditional medicinal plant from Polynesia widely used for the treatment of many diseases including arthritis, diabetes, asthma, hypertension and cancer. Here, a commercial noni juice (TNJ) was evaluated for its protective activities against the lesions induced by mitomycin C (MMC) and doxorrubicin (DXR) using the Somatic Mutation and Recombination Test (SMART) in Drosophila melanogaster. Three-day-old larvae, trans-heterozygous for two genetic markers (mwh and flr3      ), were co-treated with TNJ plus MMC or DXR. We have observed a reduction in genotoxic effects of MMC and DXR caused by the juice. TNJ provoked a marked decrease in all kinds of MMC- and DXR-induced mutant spots, mainly due...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxicity; Doxorrubicin; Mitomycin C; SMART; Noni juice.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000200585

Protective effects of steroidal alkaloids isolated from Solanum paniculatum L. against mitomycin cytotoxic and genotoxic actions
Provedor de dados: 42
Autores: VIEIRA,PABLINE M.; MARINHO,LORENA P.M.; FERRI,SUZANA C.S.; CHEN-CHEN,LEE.
Solanum paniculatum L. is a plant species widespread throughout tropical America, especially in the Brazilian Cerrado region. It is used in Brazil for culinary purposes and in folk medicine to treat liver and gastric dysfunctions, as well as hangovers. Previous studies with S. paniculatum ethanolic leaf extract or ethanolic fruit extract demonstrated that they have no genotoxic activity neither in mice nor in bacterial strains, although their cytotoxicity and antigenotoxicity were demonstrated in higher doses. In order to assess the possible compounds responsible for the activities observed, we fractionated the ethanolic fruit extract of S. paniculatum, characterized by 1H and 13C NMR spectra, and evaluated two fractions containing steroidal alkaloids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticytotoxicity; Antigenotoxicity; Jurubeba; Micronuclei; Solanaceae.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000200553

Hibiscus acetosella extract protects against alkylating agent-induced DNA damage in mice
Provedor de dados: 42
Autores: VILELA,THAIS C.; LEFFA,DANIELA D.; DAMIANI,ADRIANI P.; DAMAZIO,DAIANE DAL COL; MANENTI,ALINE V.; CARVALHO,TIAGO JOSÉ G.; RAMLOV,FERNANDA; AMARAL,PATRICIA A.; ANDRADE,VANESSA M. DE.
Abstract Hibiscus acetosella was shown to exert beneficial effects in humans and animal models however, the effects of this plant on DNA are unknown. The aim of this study was to determine the antigenotoxic and antimutagenic effects of H. acetosella extracts on alkylating agent methyl methanesulfonate (MMS) in vivo in mice. Initially, we performed analysis of phenolic compounds in extracts of H. acetosella by high-performance liquid chromatography (HPLC). Next, mice were divided into 8 groups and treated with distilled water or plant extract (0.1 ml/10 g) by gavage for 15 days, followed by intraperitoneal (ip) administration of saline solution or MMS (40 mg/Kg b.w) on day 16. Caffeic acid, following by gallic acid, gallocatechin, coumaric acid, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hibiscus acetosella; Antigenotoxicity; Comet assay; Antimutagenicity; Micronucleus test.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603165

Brown Flaxseed Prevents DNA Damage Induced by 1,2-Dimethylhydrazine in a Pre-Clinical Model
Provedor de dados: 52
Autores: Pamplona-Silva,Maria Tereza; Morandi,Walter Vinicius; Bernardi,Luana; Tura,Bethânia Borges; Oliveira,Diego Duarte Marques de; Antoniolli-Silva,Andreia Conceição Milan Brochado; Mauro,Mariana de Oliveira; Oliveira,Rodrigo.
ABSTRACT The study evaluated the effects of brown flaxseed supplementation in natura on the prevention of DNA damage induced by 1,2-dimethylhydrazine (DMH) in vivo. The experimental groups were Negative and Positive Controls and the protocols of Pre-treatment, Simultaneous, Post-treatment, Pre+continuous in relation to the supplementation of brown flaxseed and administration with carcinogenic compound. The results showed that brown flaxseed supplementation does not cause genomic and genetic damage. In addition, brown flaxseed showed a chemopreventive food that reduced the damages assessed by the comet assay up to 94.07x and the damages assessed by the micronucleus assay up to 91.88x. Brown flaxseed supplementation also increased the frequency of monocytes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flaxseed; Antigenotoxicity; Chemoprevention; Mice.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100316