Consulta: subjectFacets:"Artificial seawater"
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Data/hora: 03/06/2024 06:28:12

Detection of cytotoxin-hemolysin mRNA in nonculturable populations of environmental and clinical Vibrio vulnificus strains in artificial seawater
Provedor de dados: 5
Autores: Fischer Le Saux, Marion; Hervio Heath, Dominique; Loaec, Solen; Colwell, Rita; Pommepuy, Monique.
The objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of Vibrio vulnificus. For that purpose, we applied seminested reverse transcription-PCR (RT-PCR) to viable but nonculturable (VBNC) populations of V. vulnificus and targeted the cytotoxin-hemolysin virulence gene vvhA. Three strains, two environmental, IF Vv10 and IF Vv18, and one clinical, C7184, were used in this study. Artificial seawater, inoculated with mid-log-phase cells, was maintained at 4degreesC. VBNC cells resulted after 3, 6, and 14 days for C7184, IF Vv18, and IF Vv10, respectively. Our data indicate that seminested RT-PCR is sensitive for the detection of vvhA mRNA in artificial seawater when...
Tipo: Text
Palavras-chave: Artificial seawater; Reverse transcription PCR; Cytotoxin Hemolysin; Molecular detection method; Vibrio vulnificus.
Ano: 2002
URL: https://archimer.ifremer.fr/doc/2002/publication-1269.pdf