Consulta: subjectFacets:"Aspergillus niger"
Registros recuperados: 53
Data/hora: 01/06/2024 02:40:01

Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261
Provedor de dados: 69
Autores: Cavalcanti Luna,Marcos A; Rodrigues Vieira,Edson; Okada,Kaoru; Campos-Takaki,Galba Maria; do Nascimento,Aline Elesbão.
Background The effects of exposure to copper, during growth, on the production of biomass, total protein, catalase, glutathione-S transferase, glutathione peroxidase, peroxidase, polyphosphate, acid and alkaline phosphatases, ultrastructure and the ability to remove this metal from Aspergillus niger, obtained from caatinga soil, were evaluated. Results All parameters tested were influenced by the concentration of metal in the culture medium. The presence of metal induced high levels of antioxidant enzymes, including lipid peroxidation, thereby revealing the appearance of an oxidative stress response. The variation in polyphosphate levels indicates the participation of the polymer in response to stress induced by copper. The activities of the phosphatases...
Tipo: Journal article
Palavras-chave: Aspergillus niger; Antioxidant enzyme; Copper removal; Polyphosphate; Ultrastructure.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600006

Bioconversion of apple pomace into a multienzyme bio-feed by two mixed strains of Aspergillus niger in solid state fermentation
Provedor de dados: 69
Autores: Zhong-Tao,Sun; Lin-Mao,Tian; Cheng,Liu; Jin-Hua,Du.
Apple pomace is a wasted resource produced in large quantities and its deposit has caused serious environmental problems, so it is significance to make the full utilization of the residues. The objectives of this work were to produce multienzyme bio-feed, biodegrade the anti-nutritional factors such as pectin and tannins in apple pomace, and obtain the nutritional enrichment of the fermented substrate. The mixture of apple pomace and cottonseed powder (1:1, w/w), supplemented with 1% (w/w) (NH4)2SO4 and 0.1% (w/w) KH2PO4, was proved to be the optimum medium for the mixed strains of Aspergillus niger M2 and M3 (2:1, w/w). The activities of pectinase, proteinase and cellulase achieved 21168 u/g, 3585 u/g and 1208u/g, and the biodegradation rates of pectin...
Tipo: Journal article
Palavras-chave: Apple pomace; Aspergillus niger; Multienzyme bio-feed; Pectin; Solid state fermentation; Tannins.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100002

Construction and expression of two-copy engineered yeast of feruloyl esterase
Provedor de dados: 69
Autores: Zhou,Min; Huang,Zhiqing; Zhou,Bo; Luo,Yanliu; Jia,Gang; Liu,Guangmang; Zhao,Hua; Chen,Xiaoling.
Background Aspergillus niger has the ability to secrete feruloyl esterase. However, for economically viable industrial applications, it is necessary to increase their catalytic activities and/or protein yields to satisfy the increasing needs for feruloyl esterases. Results The gene AnFaeA that encodes a type A feruloyl esterase was successfully expressed in Pichia pastoris by a two-copy engineered yeast. After a screen in shaker flask, a one-copy strain GSKFA3 having the highest feruloyl esterase activity of 2.4 U/mL was obtained. Then, the pPICZaA-AnFaeA plasmid was transformed into GSKFA3 and the transformants were grown on YPDS plates with antibiotic Zeocin. After cultivation, a two-copy strain GSKZaFA20 with the highest feruloyl esterase activity of...
Tipo: Journal article
Palavras-chave: Aspergillus niger; Feruloyl esterase; Pichia pastoris.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500002

A new approach to chemical modification protocols of Aspergillus niger and sorption of lead ion by fungal species
Provedor de dados: 69
Autores: Rafiu Awofolu,Omotayo; Okechukwu Okonkwo,Jonathan; Roux-Van Der Merwe,Renate; Badenhorst,Jackie; Jordaan,Erika.
Biomasses of five fungi species (Aspergillus niger, Penicillium austurianum, Saccharomyces cerevisiae, Mucor arcindloides and Trichoderma reesi were evaluated for their uptake of lead ion from aqueous solution using batch systems. Both dead and live fungal biomasses were comparatively studied for their adsorption efficiencies. The effect of pH as one of the primary factors that influences sorption efficiency of metal ions in solution was also studied. Modification of the fungal biomass with the least sorption capacity was carried out using a four-step procedure. Three different modifying agents under the optimised experimental conditions were used. The percentage uptake of lead ion by fungi species ranged from: Aspergillus niger: 6.71-64.95% and...
Tipo: Journal article
Palavras-chave: Aspergillus niger; Fungi species; Lead ion; Modification; Sorption.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400001

Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants
Provedor de dados: 69
Autores: De Nicolás-Santiago,Soledad; Regalado-González,Carlos; García-Almendárez,Blanca; Fernández,Francisco J; Téllez-Jurado,Alejandro; Huerta-Ochoa,Sergio.
Mutant strains from Aspergillus niger UAM-GS1 were produced by UV radiation to increase their hemicellulolytic and cellulolytic activity production. The mutant strains showing more enzymatic activity were those labelled GS1-S059 and GS1-S067. These strains also showed the largest relationship between diameter of hydrolysis zone and colony diameter. The mutant GS1-S067 showed a colony radial extension rate and a biomass growth rate g biomass/(cm² h), 1.17 times higher than that achieved by strain UAM-GS1. The high invasive capacity makes this mutant strain a promising alternative for its use in solid substrate fermentation (SSF). The morphological properties of the two mutant strains were evaluated by using scanning electron microscopy. The diameter of the...
Tipo: Journal article
Palavras-chave: Aspergillus niger; Mutation; Mannanase production.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100008

Use of Aspergillus niger in the bioleaching of colemanite for the production of boric acid
Provedor de dados: 69
Autores: Bayat,Oktay; Arslan,Volkan; Bayat,Belgin.
Colemanite is one of the most important underground riches of Turkey, having approximately 60% of the world boron deposits, and it has a large portion in the deposits. In this study, chemical leaching and biological leaching methods were used for production of boric acid from colemanite (2CaO Â· 3B3O3 Â· 5H2O) (Emet-KÃ¼tahya, Turkey). Oxalic acid concentration, temperature, stirring time and solid-to-liquid ratio were taken as parameters in the chemical leaching process. It was found that the dissolution rate increases with increasing oxalic acid concentration and temperature but it decreases at higher solid-to-liquid ratios in the chemical leaching process. Using optimum conditions (d100 = 0.075 mm; 5% solids by weight; 0.55 M oxalic acid; 80 Â± 2ÂºC...
Tipo: Journal article
Palavras-chave: Aspergillus niger; Bioleaching; Boric acid; Chemical leaching; Colemanite; Oxalic acid.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300007

Ocorrência de fumonisinas em milho armazenado em propriedades familiares da região Central de Minas Gerais.
Provedor de dados: 14
Autores: QUEIROZ, V. A. V.; ALVES, G. L. de O.; CONCEIÇÃO, R. R. P. da; MENDES, S. M.; COSTA, R. V. da; RIBEIRO, P. E. de A.
bitstream/item/95159/1/circ-198.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Aspergillus niger; Fungo; Micotoxina; Fungi; Mycotoxins.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/975580

Detecção de fungos filamentosos produtores de ocratoxina utilizando a reação em cadeia DNA polimerase.
Provedor de dados: 14
Autores: OLIVEIRA, E. M. M.; FREITAS-SILVA, O.
Introdução. Ocratoxinas. Detecção de espécies fúngicas produtoras de ocratoxina A utilizando a reação em cadeia da DNA polimerase (PCR). Referências bibliográficas.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Polimerase; Fungo; Ocratoxina; Aspergillus niger.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/888519

EFFECTIVENESS OF THE APPLICATION OF ARBUSCULAR MYCORRHIZA FUNGI AND ORGANIC AMENDMENTS TO IMPROVE SOIL QUALITY AND PLANT PERFORMANCE UNDER STRESS CONDITIONS
Provedor de dados: 126
Autores: Medina,Almudena; Azcón,Rosario.
Plant growth is limited in arid and/or contaminated sites due to the adverse conditions coming from heavy metal (HM) contamination and/or water stress. Moreover, soils from these areas are generally characterised by poor soil structure, low water-holding capacity, lack of organic matter and nutrient deficiency. In order to carry out a successful re-afforestation, it is necessary to improve soil quality and the ability of plants species to resist this harsh environment. The symbiosis with arbuscular mycorrhizal (AM) fungi has been proposed as one of the mechanisms of plant heavy metal tolerance and water stress avoidance. On the other hand, addition of organic amendments to the soil can reverse degradation of soil properties. Agro-waste residues such as dry...
Tipo: Journal article
Palavras-chave: Arbuscular mycorrhiza (AM); Fungi; Organic amendment; Heavy metal (HM) contamination; Water stress; Drought; Organic matter; Rock phosphate; Revegetation; Bioremediation; Aspergillus niger; Phosphate solubilization.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162010000100009

Solubilização do fosfato de ferro em meio de cultura
Provedor de dados: 86
Autores: Barroso,Cinthya Babá; Nahas,Ely.
O objetivo deste trabalho foi avaliar a eficiência de solubilização de FePO4 por Aspergillus niger, em meio de cultura com diferentes fontes de carbono (C) e nitrogênio (N) e com concentrações crescentes de fosfato. A quantidade de fosfato solúvel, acidez e pH final foram determinados após o crescimento do fungo, em cultura estacionária a 30ºC. A eficiência de solubilização aumentou conforme o crescimento do fungo, atingiu o máximo no 11º dia (68%) e depois regrediu. Das fontes de C e de N testadas, as maiores eficiências de solubilização foram obtidas com manitol (21%) e ácido glutâmico (17%). Com o aumento da concentração de fosfato (0 a 1.330 µg mL-1), a máxima eficiência de solubilização (70%) foi obtida com 330 µg mL-1 de PO4(3-) e decresceu até 47%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Acidez titulável; Fontes de carbono; Fontes de nitrogênio; Fosfato solúvel.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000400012

The effects of inorganic salts on biosynthesis of pectinolytic enzymes by Aspergillus Niger
Provedor de dados: 31
Autores: Mojsov,   Kiro.
The paper examines effects of different inorganic salts in an apple pulp base on the production of pectinolytic enzymes with the aim of optimizing the medium for maximal enzyme production. The apple pulp  combined with corn flour and simple mineral salts was used as a nourishing base in submerged production of  pectinolytic enzymes by the  fungus Aspergillus niger MK-15. The growth of the microorganism (dry weight) on different sources of nitrogen showed  maximum dry weight with (NH4)2HPO4. The growth of the microorganism (dry weight) on different concentration of (NH4)2HPO4  (by 0.2% to 0.8%) provided maximal dry weight with 0.7% (NH4)2HPO4. The different inorganic salts (sources of nitrogen) on base  stimulated the production of pectinolytic enzymes and...
Tipo: Journal Article
Palavras-chave: Apple pulp; Fermentation; Inorganic salts; Pectinolytic enzymes; Aspergillus niger; Agribusiness; Research and Development/Tech Change/Emerging Technologies; L65.
Ano: 2010
URL: http://purl.umn.edu/94732

Caracterização da poligalacturonase produzida por fermentação semi-sólida utilizando-se resíduo do maracujá como substrato
Provedor de dados: 91
Autores: Souza,Rosane L. A. de; Oliveira,Líbia de S. C.; Silva,Flávio L. H. da; Amorim,Beatriz C.
As enzimas pectinolíticas ou pectinases são um grupo heterogêneo de enzimas que hidrolisam as substâncias pécticas presentes nas células vegetais. Essas enzimas estão sendo aplicadas em diversas áreas tornando-se importante conhecer suas características visando uma aplicação eficiente. Assim, este trabalho, objetivou-se caracterizar o resíduo seco da casca e do albedo do maracujá (Passiflora edulis) e posterior caracterização da enzima poligalacturonase, produzida através da fermentação em estado sólido e, como agente da fermentação, o fungo filamentoso Aspergillus niger. O resíduo do maracujá apresenta teor de pectina de 13,10%. A atividade de poligalacturonase alcançou um pico em 66 h de processo, para 40% de umidade inicial e 1% da concentração da fonte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora edulis; Pectinases; Aspergillus niger; Pectina.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662010000900011

Isotermas de adsorção do pedúnculo seco do caju
Provedor de dados: 91
Autores: Alcântara,Siumara R.; Almeida,Francisco de A. C.; Silva,Flávio L. H. da; Gomes,Josivanda P.
A atividade de água constitui um fator importante no processo de fermentação semi-sólida, haja vista sua relação com a quantidade de água disponível ao microrganismo responsável pelo metabolismo do produto, sendo necessário à obtenção de isotermas de sorção para caracterização do substrato. Ante o exposto, objetivou-se a construção das isotermas de adsorção do pedúnculo seco do caju (Anacardium occidentale L.) nas temperaturas usuais de fermentação (25, 30, 35 e 40 ºC). Ajustaram-se as isotermas com os modelos de BET, GAB, Oswin, Henderson e Smith. Observou-se que o modelo de GAB apresentou melhor ajuste, de vez que, na faixa de atividade de água que maximiza a biossíntese do microrganismo, para produção de pectinases por Aspergillus niger, a umidade do...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atividade de água; Anacardium occidentale L.; Fermentação semi-sólida; Pectinase; Aspergillus niger.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662009000100012

Lixiviação de poligalacturonases obtidas pela fermentação semissólida da casca e albedo do maracujá-amarelo
Provedor de dados: 91
Autores: Sousa,Carlos A. B. de; Silva,Flávio L. H. da; Conrado,Líbia de S.
Obtiveram-se poligalacturonases por fermentação semissólida usando-se, como substrato, o resíduo agroindustrial do maracujá-amarelo (Passiflora edulis flavicarpa) e, como agente da fermentação, o fungo filamentoso Aspergillus niger mutante CCT 0916. Para otimizar a etapa de recuperação das enzimas os parâmetros da lixiviação: agitação, tempo de contato e relação solvente/substrato, foram investigados em um planejamento experimental fatorial 2³. Agitação de 50 rpm, 45 min e relação 10:1 proporcionaram a melhor recuperação de enzimas em que os resultados avaliados por meio da metodologia de superfície de resposta, mostraram que o resíduo do maracujá é um substrato adequado para a produção de poligalacturonases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recuperação; Extração; Aspergillus niger; Resíduo agroindustrial.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662012000700013

Production and quantitation of cellulases by solid state fermentation residues of agroindustrial
Provedor de dados: 22
Autores: Santos, Tamires Carvalho dos; Abreu Filho, George; Rocha, Thiago José Honório; Ferreira, Adriana Nascimento; Diniz, Gleiza Alves; Franco, Marcelo.
The objective of this study was to analyze and quantify the activity of enzymes endoglucanase and total cellulases produced by solid state fermentation of  waste from processing of cocoa, mango and  hog plum, used  the fungus Aspergillus niger as inoculant. To determine the effect of  enzyme production, an experiment was conducted in a factorial 3 x 5, in which three levels of water content (40, 50 and  60%) and five levels for the fermentation time (24, 48, 72 , 96 and 120 h). Fermentation was performed  at  30 °C in a bacteriological incubator refrigerated. The industrial residues shown to be highly effective in the production of enzymes from the solid-state fermentation, especially production of  endoglucanse residue from the sleeve has reached  the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Biotransformação; Modelos matemáticos significativos.
Ano: 2013
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/6902

Production and action pattern of inulinase from Aspergillus Niger-245: hydrolysis of inulin from several sources
Provedor de dados: 59
Autores: Cruz,Vinícius D’Arcadia; Belote,Juliana Gisele; Belline,Márcia Zilioli; Cruz,Rubens.
A strain of Aspergillus niger isolated from soil samples showed great capacity to produce extracellular inulinase. Although the enzyme has been synthesized in presence of monosaccharides, sucrose and sugar cane molasse, the productivity was significantly higher (p&amp;lt;0.05) when the microorganism was inoculated in media formulated with dahlia extract and pure inulin, as carbon sources. With regard to the nitrogen source, the best results were obtained with casein and other sources of proteic nitrogen, comparatively to the mineral nitrogen. However, statistic significance (p&amp;lt;0.01) only was found between the productivity obtained in the medium prepared with casein and ammonium sulphate. The optimum pH of the purified enzyme for inulin hydrolysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inulinase; Aspergillus niger; Fructose syrup; Inulin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400013

Intra and extracellular nuclease production by Aspergillus niger and Aspergillus nidulans
Provedor de dados: 59
Autores: Ferreira,Adlane V. B.
Intra and extracellular nuclease production by strains of Aspergillus niger and Aspergillus nidulans was estimated using a modified DNAse test agar and cell-free extract assays. Differences in the production of nucleases by A. niger and A. nidulans were observed. These observations suggest that the DNAse test agar can be helpful for a quick screening for some types of nucleases in filamentous fungi. The assays using cell-free extracts can also be useful for initial characterization of other types of nucleases.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nucleases; Aspergillus niger; Aspergillus nidulans; Filamentous fungi.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400009

Resposta à inoculação de fungo solubilizador de fósforo em milho
Provedor de dados: 63
Autores: Nahas,E.; Fornasieri,D. J.; Assis,L. C.
Este trabalho procurou verificar o efeito da adição de duas fontes de fosfato prontamente disponíveis, superfosfato triplo e fosfato solubilizado obtido por via microbiológica e uma fonte não prontamente disponível, apatita de Araxá, na cultura do milho. Em adição, foi verificado o efeito da inoculação do fungo Aspergillus niger, solubilizador de fosfato de rocha e da adição de matéria orgânica. Nos tratamentos em que se usou matéria orgânica, houve um aumento de população microbiana total do solo, mas nenhum efeito foi observado na produção e absorção de fósforo pela cultura do milho. Tanto o superfosfato triplo como a apatita de Araxá permitiram resultados mais favoráveis em termos de produção de massa seca e absorção de fósforo pelo milho em relação ao...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solubilização de fosfato de rocha; Aspergillus niger; Milho.
Ano: 1994
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161994000300014

Manipueira como substrato na biossíntese de ácido cítrico por Aspergillus niger
Provedor de dados: 63
Autores: Leonel,M.; Cerada,M.P.
O trabalho teve por objetivo avaliar a viabilidade do uso da manipueira, residuo líquido resultante da prensagem da massa ralada de mandioca, como substrato na biossíntese de ácido cítrico por Aspergillus niger. Os meios de manipueira foram comparados nas mesmas condições de temperatura, a meios sintéticos, utilizados tradicionalmente. Em se tratando de proposta de um novo substrato, foi estudado o armazenamento do resíduo a temperatura ambiente por 72 horas, e realizada a caracterização físico-química da manipueira e dos meios elaborados com esse substrato. Foi avaliada a produção de ácido cítrico nos meios sintéticos e de manipueira. Verificou-se que a produção de ácido cítrico não diferiu quanto ao meio. Não foi observado crescimento do microrganismo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ácido cítrico; Aspergillus niger; Substrato; Manipueira; Resíduo de mandioca.
Ano: 1995
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000200016

Efecto del fósforo y del potasio en la producción de ácido cítrico utilizando una cepa de Aspergillus niger: Effect of P and K in the citric acid production using an Aspergillus niger strain
Provedor de dados: 109
Autores: Gómez Schouben,Ana Lucía; Sánchez de Prager,Marina; Muñoz,Jaime Eduardo; Valencia,Isabel Cristina.
En la investigación se evaluaron el rendimiento (%) y la productividad (g/lt por hora) de la fermentación sumergida de ácido cítrico con el uso de una cepa de Aspergillus niger mejorada por exposición a luz UV, cuando se incrementa el nivel de potasio (K) y reduce el nivel de fósforo (P) en una formulación de base que contiene sacarosa, micro- y macronutrientes. Se evaluaron cinco tratamientos en un diseño completamente al azar con tres repeticiones en una estructura multifactorial completa aumentada 2² + punto central. Los mejores resultados de rendimiento y productividad se obtuvieron con 329 mg/ lt de P y 271 mg/lt de K (tratamiento 2), equivalentes a 63.2% de rendimiento y 1.20 g/lt por hora de productividad. Se encontró que sólo el potasio es...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Ácido cítrico; Fermentación; Macronutrientes; Fósforo; Potasio.
Ano: 2014
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122014000300004

In vitro DISSOLUTION OF ACIDULATED ROCK PHOSPHATE BY PHOSPHATE SOLUBILIZING MICROORGANISMS
Provedor de dados: 37
Autores: MORENO QUEVEDO,Ángela Patricia; OSORIO VEGA,Nelson Walter; GONZÁLEZ MURILLO,Octavio Augusto.
The low availability of phosphorus (P) in the soil and the high cost of P fertilization are factors that limit agricultural productivity. A biotechnological alternative for to handle this problem is to use soil microorganisms capable of dissolving rock phosphate (RP), thus improving its effectiveness as a P fertilizer. This study was carried out with the objective of determining the effectiveness of Aspergillus niger -As-, Penicillium sp. -Pn-, Bacillus sp -B-. and an unidentified actinomycete -At- in the in vitro dissolution of two partially acidulated rock phosphates. The treatments consisted of 2x16 factorial arrangement [2 levels of RP: either Boyaca RP or Norte de Santander RP; 16 levels of inoculum: an uninoculated control, individual inoculations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Bacillus sp; Biofertilizer; Phosphate; Penicillium sp.
Ano: 2015
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000200007

Reuse of wastewater from pulp industry for the optimization of fungal xylanase production
Provedor de dados: 3
Autores: Queiroz-Fernandes, Geisiany Maria de; Martins, Bruna Letícia; Rustiguel, Cynthia Barbosa.
The production of enzymes using agro-industrial waste is a low cost alternative for the reuse of byproducts, with the subsequent impact decrease on the environment. Current analysis produced xylanase using fungus Aspergillus niger, with two types of wastewater generated during the pulp chemical bleaching phase as inducers. Xylanase was produced by submerged liquid fermentation and factorial design optimized parameters that influence production (concentration of wastewater and production period). Initial culture conditions (pH, temperature and agitation) were optimized independently. Alkaline wastewater was more effective than acidic wastewater for the induction of xylanase in optimized conditions: 50% of culture medium, 7-day production, 30°C, pH 6.0 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 90400003 enzyme; Aspergillus niger; Factorial design; Agro-industrial waste; Xylanase. Biotecnologia.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34459

Production and characterization of endo-polygalacturonase from Aspergillus niger in solid-state fermentation in double-surface bioreactor
Provedor de dados: 52
Autores: Hendges,Diogo Henrique; Montanari,Queli; Malvessi,Eloane; Silveira,Mauricio Moura da.
Endo-polygalacturonase (endo-PG) production by Aspergillus niger T0005/007-2 in solid medium with 170 mm of height was evaluated in a cylindrical double surface bioreactor in 96-h experiments. Cell concentration close to 92 mg.g -¹ dm (mg per g of dry medium) in the standard condition (static) was achieved, whereas in tests under forced aeration of 1.4 and 2.8 L.min-1. Kg-1 mm (L of air per minute per Kg of moist medium) and with the central shaft fungal biomass attained approximately 100 mg.g-1 dm. Superior endo-PG activity was obtained with the central-shaft system, 78 U.g-1 dm (units per g of dry medium). Forced aeration and pressure pulse showed no positive effect on the production of endo-PG, 45 U.g-1 dm and 28 U.g-1 dm, respectively. None of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Double-surface bioreactor; Endo-polygalacturonase; Solid-state fermentation; Enzyme stability.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200005

Influence of agitation speeds and aeration rates on the Xylanase activity of Aspergillus niger SS7
Provedor de dados: 52
Autores: Bakri,Yasser; Mekaeel,Alaa; Koreih,Amal.
In this study, the effect of agitation and aeration rates on xylanase activity of Aspergillus niger SS7 in 3-litre stirred tank bioreactor was investigated. The agitation rates tested were 100, 200 and 300 rpm at each airflow rates of 0.5, 1.0 and 1.5 vvm. The maximum xylanase activity in mono- agitator system was at the agitation speed of 200 rpm and aeration rate of 1.0 vvm. In bi-agitator system, at low agitation speed (100 rpm), the xylanase activity was enhanced by 13% compared to mono- agitator system for an aeration rate of 1.0 vvm. Xylanase productivity in continuous culture was higher by approximately 3.5 times than in batch culture.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Agitation; Aeration; Xylanase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400003

Microbial production of citric acid
Provedor de dados: 52
Autores: Vandenberghe,Luciana P. S; Soccol,Carlos R; Pandey,Ashok; Lebeault,Jean-Michel.
Citric acid is the most important organic acid produced in tonnage and is extensively used in food and pharmaceutical industries. It is produced mainly by submerged fermentation using Aspergillus niger or Candida sp. from different sources of carbohydrates, such as molasses and starch based media. However, other fermentation techniques, e.g. solid state fermentation and surface fermentation, and alternative sources of carbon such as agro-industrial residues have been intensively studied showing great perspective to its production. This paper reviews recent developments on citric acid production by presenting a brief summary of the subject, describing micro-organisms, production techniques, and substrates, etc.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citric acid; Aspergillus niger; Submerged fermentation; Solid state fermentation; Substrates.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300001

Improving Aspergillus niger tannase yield by N+ ion beam implantation
Provedor de dados: 52
Autores: Jin,Wei; Nie,Guangjun; Liu,Hui; Xiaoran,Yang; Gong,Guohong; Wang,Li; Zheng,Zhiming.
This work aimed to improve tannase yield of Aspergillus niger through N+ ion beam implantation in submerged fermentation. The energy and dose of N+ ion beam implantation were investigated. The results indicated that an excellent mutant was obtained through nine successive implantations under the conditions of 10 keV and 30-40 (×2.6×10(13)) ions/cm², and its tannase yield reached 38.5 U/mL, which was about five-time higher than the original strain. The study on the genetic stability of the mutant showed that its promising performance in tannase production could be stable. The studies of metal ions and surfactants affecting tannase yield indicated that manganese ions, stannum ions, xylene and SDS contained in the culture medium had positive effects on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; N+ ion implantation; Tannase.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100018

Studies on production of single cell protein by Aspergillus niger in solid state fermentation of rice bran
Provedor de dados: 52
Autores: Anupama,; Ravindra,Pogaku.
An attempt was made to apply the solid state fermentation (SSF) for the production of single cell protein (SCP) using oil free rice bran waste as substrate. A local isolate of Aspergillus niger, was used as protein source for the studies. Total proteins were extracted to estimate the mycelial biomass from the moldy bran. Carbonate-bicarbonate extraction buffer and a pH 10 was found to be most efficient among the buffers used for the extraction of the proteins from the organism. The effect of supplementation by various sources of nitrogen and mineral solution on the final biomass yield was compared. The influence of C/N ratio on the protein yield was also studied. Sodium nitrate at C/N ratio of 1.387 was found to be an effective nitrogen-supplementing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single cell protein; Solid State Fermentation; Aspergillus niger; Rice bran; C/N ratio.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100011

Evaluation of Antimicrobial Activity of Glucose Oxidase from Aspergillus niger EBL-A and Penicillium notatum
Provedor de dados: 52
Autores: Zia,Muhammad Anjum; Riaz,Ayesha; Rasul,Samreen; Abbas,Rao Zahid.
This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and Penicillium notatum using corn steep liquor as the substrate and evaluate its antimicrobial activity for use in pharmaceutical and food industries. The enzyme was purified by ammonium sulfate precipitation (60-85%), DEAE-cellulose ion exchange and Sephadex G-200 size exclusion chromatography. The crude enzyme extracts of A. niger and P. notatum showed 2.32 and 5.53 U mg-1 specific activities, respectively, which after desalting was 15.52 and 12.05 U mg-1, and after ion exchange and gel filtration chromatography was 29.09 - 62 and 25.72 - 59.37 U mg-1 for A. niger and P. notatum, respectively. The antimicrobial activity was determined by disc diffusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose oxidase; Antimicrobial; Aspergillus niger; Penicillium notatum.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600010

Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
Provedor de dados: 52
Autores: Singh,Jagdish; Verma,Neelam.
The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Glucose oxidase; Aqueous two phase.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500007

Production of pectinases by A. niger: influence of fermentation conditions
Provedor de dados: 52
Autores: Martos,María A.; Martinez Vazquez,Francisco; Benassi,Fernando O.; Hours,Roque A.
Response surface methodology was used for optimization of polygalacturonase (PG) and pectinesterase (PE) production in submerged fermentation by A.niger. A Central Composite Experimental Design was applied, consisting of 22 experiments, including eight central points. Variables studied were: fermentation time (24 to 120 h), pH (3.5 to 6.5) and initial concentration of pectin (5 to 20 g/l). Maximum PE production was 220 U/l, after 74 h of culture, in a medium containing 20 g/l of pectin (pH 6.5). The optimal conditions for PG production were pH: 4.1, 20 g/l of pectin and 94 h of fermentation with a maximum value of 1032 U/l. Under these conditions, the PE production was low (15 U/l). A liquid extract with high PG activity and low PE activity could be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectinases; Polygalacturonase; Pectinesterase; Aspergillus niger; Response surface methodology; Citrus pectin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300007

Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation
Provedor de dados: 52
Autores: Slivinski,Christiane Trevisan; Machado,Alex Vinicius Lopes; Iulek,Jorge; Ayub,Ricardo Antônio; Almeida,Mareci Mendes de.
In this work, glucoamylase was produced by Aspergillus niger in solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and ion exchange and gel filtration chromatographies. Its molecular mass was estimated as 118.17 kDa by electrophoresis. The partially purified enzyme had an optimum pH range of 4.5-5.0 and an optimum temperature of 60 °C, with average activity 152.85 U mL-1. Thermal and pH stability assays with the crude extract showed that more than 60 % of the activity remained at pH 4.6 and 60 °C, even after an exposition to these conditions longer than 24 h. Yet, after purification, the enzyme was stable at these for at least 4 h, which indicated that its purification for use in starch saccharification was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucoamylase; Aspergillus niger; Solid-state fermentation; Biochemical characterization; Purification.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300018

Purification and characterization of β-Fructosidase with inulinase activity from Aspergillus niger - 245
Provedor de dados: 52
Autores: Cruz,Vinícius D'Arcadia; Belote,Juliana Gisele; Dorta,Claudia; Santos,Luíza Helena Oliveira dos; Andriolo,Cláudia Regina; Khenayfes,Marcelo de Oliveira; Cruz,Rubens.
Aspergillus niger - 245, a strain isolated from soil samples showed good β-fructosidase activity when inoculated in medium formulated with dahlia extract tubers. The enzyme was purified by precipitation in ammonium sulphate and percolated in DEAE-Sephadex A-50 and CM-cellulose columns, witch showed a single peack in all the purification steps, maintaining the I/S ratio between 0.32 to, 0.39. Optimum pH for inulinase activity (I) was between 4.0 - 4.5 and for invertase activity (S) between 2.5 and 5.0. The optimum temperature was 60O.C for both activities and no loss in activity was observed when it was maintained at this temperature for 30 min. The Km value was 1.44 and 5.0, respectively, for I and S and Vm value 10.48 and 30.55, respectively. The I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inulinase; Invertase; Β-fructosidase; Inulin; Fructose syrup; Aspergillus niger.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300003

Citric acid production from orange peel wastes by solid-state fermentation
Provedor de dados: 58
Autores: Torrado,Ana María; Cortés,Sandra; Salgado,José Manuel; Max,Belén; Rodríguez,Noelia; Bibbins,Belinda P; Converti,Attilio; Domínguez,José Manuel.
Valencia orange (Citrus sinensis) peel was employed in this work as raw material for the production of citric acid (CA) by solid-state fermentation (SSF) of Aspergillus niger CECT-2090 (ATCC 9142, NRRL 599) in Erlenmeyer flasks. To investigate the effects of the main operating variables, the inoculum concentration was varied in the range 0.5·10³ to 0.7·10(8) spores/g dry orange peel, the bed loading from 1.0 to 4.8 g of dry orange peel (corresponding to 35-80 % of the total volume), and the moisture content between 50 and 100 % of the maximum water retention capacity (MWRC) of the material. Moreover, additional experiments were done adding methanol or water in different proportions and ways. The optimal conditions for CA production revealed to be an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orange peel; Citric acid; Aspergillus niger; Solid-state fermentation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100049

Characterization And Application Of Tannase Produced By Aspergillus Niger ITCC 6514.07 On Pomegranate Rind
Provedor de dados: 58
Autores: Srivastava,Anita; Kar,Rita.
Extracellular tannase and gallic acid were produced optimally under submerged fermentation at 37 0C, 72 h, pH 5.0, 10 %(v/v) inoculum and 4 %(w/v) of the agroresidue pomegranate rind (PR) powder by an Aspergillus niger isolate. Tannic acid (1 %) stimulated the enzyme production by 245.9 % while with 0.5 % glucose, increase was marginal. Tannase production was inhibited by gallic acid and nitrogen sources such as NH4NO3, NH4Cl, KNO3, asparatic acid, urea and EDTA. The partially purified enzyme showed temperature and pH optima of 35 0C and 6.2 respectively which shifted to 40 0C and 5.8 on immobilization in alginate beads. Activity of the enzyme was inhibited by Zn+2, Ca+, Mn+2, Mg+2, Ba+2and Ag+. The immobilized enzyme removed 68.8 % tannin from juice of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tannase; Pomegranate rind; Myrobalan; Aspergillus niger.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400008

Selection of 5-fluorocytosine-resistant mutants from an Aspergillus niger citric acid-producing strain
Provedor de dados: 58
Autores: Conte,Ana Paula de F.; Marin,José M.
Mutants of Aspergillus niger N402, induced by UV mutagenesis, were selected and tested for resistance or sensitivity to 5-fluorocytosine. Some mutants showed increased citric acid production, which did not correlate with the intracellular amount of protein or ammonium ion. The resistance to 5-fluorocytosine proved to be a rational approach for isolation of new mutants with improved production of citric acid. The best mutant (FR13) accumulated double the amount of citric acid produced by the parental strain.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Citric acid; 5-fluorocytosine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100001

A biodegradation study of forest biomass by Aspergillus niger F7: correlation between enzymatic activity, hydrolytic percentage and biodegradation index
Provedor de dados: 58
Autores: Sharma,Nivedita; Kaushal,Richa; Gupta,Rakesh; Kumar,Sanjeev.
Aspergillus niger F7 isolated from soil was found to be the potent producer of cellulase and xylanase. The residue of forest species Toona ciliata, Celtris australis, Cedrus deodara and Pinus roxburghii was selected as substrate for biodegradation study due to its easy availability and wide use in industry. It was subjected to alkali (sodium hydroxide) treatment for enhancing its degradation. Biodegradation of forest waste by hydrolytic enzymes (cellulase and xylanase) secreted by A. niger under solid state fermentation (SSF) was explored. SSF of pretreated forest biomass was found to be superior over untreated forest biomass. Highest extracellular enzyme activity of 2201±23.91 U/g by A. niger was shown in pretreated C. australis wood resulting in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; SSF; Enzyme activity; Percent hydrolysis; Biodegradation index.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200006

Hyperexpression of two Aspergillus niger xylanase genes in Escherichia coli and characterization of the gene products
Provedor de dados: 58
Autores: Yi,Xiuli; Shi,Yan; Xu,Hui; Li,Wei; Xie,Jie; Yu,Rongqing; Zhu,Jun; Cao,Yi; Qiao,Dairong.
The analysis of individual gene product should enable to clarify the role of a particular enzyme in a complex xylanase system of A. niger. The two genes encoding precursors of co-produced endo-1,4-β-D-xylanases, xynA1 and xynB, were isolated from Aspergillus niger SCTCC 400264 (SCTCC, China) by using RT-PCR technique and then successfully expressed in Escherichia coli BL21. The nucleotide sequences of the xynA1 and xynB genes revealed that they were only 52.5% homology to each other. Characterization of the recombinant enzymes revealed the different properties: the specific activity of recombinant XYNA1 was 16.58 U/mg compared to 1201.7 U/mg for recombinant XYNB; The optimum temperature and pH of the recombinant XYNA1 were 35 ºC and 3.0, respectively,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Xylanase; Prokaryotic expression; Enzymatic characterization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300030

Microorganisms as tools in modern chemistry: the biotransformation of 3-indolylacetonitrile and tryptamine by fungi
Provedor de dados: 58
Autores: Boaventura,Maria Amélia D.; Lopes,Rosiane F.A.P.; Takahashi,Jacqueline A.
Chemical reactions performed by microorganisms have been used as a modern tool in chemistry. In this work, the ability of the fungi Beauveria bassiana and Aspergillus niger to modify the chemical structure of indole compounds was studied. Beauveria bassiana was able to transform 3-indolylacetonitrile into 3-methylindole while Aspergillus niger transformed tryptamine into 5-hydroxyindole-3-acetamide. Interestingly, these fungi were able to perform both reduction and oxidation of the indole compounds fed, the oxidation occurring with improved levels of oxygen uptake. The results prove once more the versatility of microorganisms to perform a wide range of industrially attractive chemical reactions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotransformations; Beauveria bassiana; Aspergillus niger; 3-indolylacetonitrile; Tryptamine.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300014

Isolation and growth characterization of chlorate and/or bromate resistant mutants generated by spontaneous and induced foreword mutations at several gene loci in Aspergillus niger
Provedor de dados: 58
Autores: Kanan,Ghassan J. M.; Al-Najjar,Heyam E.
We aimed her mainly to evaluate the contribution of newly employed bromate selection system, in obtaining new Aspergillus niger nitrate/nitrite assimilation defective mutants, through Ultraviolet treatment (UV), 1, 2, 7, 8-Diepoxyoctane (DEO), phenols mixture (Phx)) and spontaneous treatments. The newly employed bromate selection system was able to specify only two putative novel mutant types designated brn (bromate resistant but chlorate sensitive (RS) strain, which may specify nitrite specific transporter) and cbrn mutants (bromate resistant and chlorate resistant strain, which may specify nitrate/nitrite bispecific system). The most relevant and innovative findings of this research work involve the isolation of the RR (cbrn) mutants (a new type of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Chlorate; Bromate; Mutagenesis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400032

Biotechnological production of citric acid
Provedor de dados: 58
Autores: Max,Belén; Salgado,José Manuel; Rodríguez,Noelia; Cortés,Sandra; Converti,Attilio; Domínguez,José Manuel.
This work provides a review about the biotechnological production of citric acid starting from the physicochemical properties and industrial applications, mainly in the food and pharmaceutical sectors. Several factors affecting citric acid fermentation are discussed, including carbon source, nitrogen and phosphate limitations, pH of culture medium, aeration, trace elements and morphology of the fungus. Special attention is paid to the fundamentals of biochemistry and accumulation of citric acid. Technologies employed at industrial scale such as surface or submerged cultures, mainly employing Aspergillus niger, and processes carried out with Yarrowia lipolytica, as well as the technology for recovering the product are also described. Finally, this review...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citric acid; Fermentation; Review; Aspergillus niger.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400005

Biosynthesis, purification and characterization of endoglucanase from a xylanase producing strain Aspergillus niger B03
Provedor de dados: 58
Autores: Dobrev,Georgi Todorov; Zhekova,Boriana Yordanova.
An extracellular endoglucanase was isolated from the culture liquid of xylanase producing strain Aspergillus niger B03. The enzyme was purified to a homogenous form, using consecutive ultrafiltration, anion exchange chromatography, and gel filtration. Endoglucanase was a monomer protein with a molecular weight of 26,900 Da determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 28,800 Da determined by gel filtration. The optimal pH and temperature values for the enzyme action were 3.5 and 65ºC respectively. Endoglucanase was stable at 40ºC, pH 3.0 for 210 min. The substrate specificity of the enzyme was determined with carboxymethyl cellulose, filter paper, and different glycosides. Endoglucanase displayed maximum activity in the case...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endoglucanase; Purification; Characterization; Aspergillus niger; Endoxylanase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100008

Growth of Aspergillus ochraceus, A. carbonarius and A. niger on culture media at different water activities and temperatures
Provedor de dados: 58
Autores: Palacios-Cabrera,Héctor; Taniwaki,Marta Hiromi; Hashimoto,Jorge Minoru; Menezes,Hilary Castle de.
The objective of this paper was to determine the influence of three culture media with different water activities, times of incubation and temperatures on the growth of A. ochraceus, A. carbonarius and A. niger. Spores of A. ochraceus, A. carbonarius and A. niger were inoculated onto three culture media: Czapeck Yeast extract Agar (CYA), Dichloran 18% Glycerol Agar (DG18) and Malt Yeast extract 40% Glucose Agar (MY40G). The plates were incubated at five different temperatures (8, 25, 30, 35 and 41ºC). The growth of fungi was evaluated every 24h, measuring the colony diameter (mm). None of the species grew at 8ºC in any culture media. For A. carbonarius, 30ºC was the best temperature for growth while for A. niger temperatures above 30ºC were better in all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus ochraceus; Aspergillus carbonarius; Aspergillus niger; Growth measurement; Water activity; Temperature.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100005

Novel agents for enzymatic and fungal hydrolysis of stevioside
Provedor de dados: 58
Autores: Milagre,H.M.S.; Martins,L.R.; Takahashi,J.A.
A comparative study on the potential of some biological agents to perform the hydrolysis of stevioside was carried out, aiming at establishing an alternative methodology to achieve the aglycon steviol or its rearranged derivative isosteviol, in high yields to be used in the preparation of novel bioactive compounds. Hydrolysis reactions were performed by using filamentous fungi (Aspergillus niger, Rhizopus stolonifer and Rhizopus arrhizus), a yeast (Saccharomyces cerevisiae)andenzymes (pancreatin and lipases PL250 and VFL 8000). Pancreatin showed the best hydrolytic activity, furnishing isosteviol at 93.9% of yield, at pH 4.0, using toluene as a co-solvent. Steviol was produced using both pancreatin at pH 7.0 (20.2% yield) and A. niger at pH 7 (20.8% yield).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stevioside; Hydrolysis; Aspergillus niger; Lipase; Pancreatin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200029

Purification and characterisation of an extracellular phytase from Aspergillus niger 11T53A9
Provedor de dados: 58
Autores: Greiner,Ralf; Silva,Lucineia Gomes da; Couri,Sonia.
An extracellular phytase from Aspergillus niger 11T53A9 was purified about 51-fold to apparent homogeneity with a recovery of 20.3% referred to the phytase activity in the crude extract. Purification was achieved by ammonium sulphate precipitation, ion chromataography and gel filtration. The purified enzyme behaved as a monomeric protein with a molecular mass of about 85 kDa and exhibited maximal phytate-degrading activity at pH 5.0. Optimum temperature for the degradation of phytate was 55°C. The kinetic parameters for the hydrolysis of sodium phytate were determined to be K M = 54 µmol l-1 and k cat = 190 sec-1 at pH 5.0 and 37°C. The purified enzyme was rather specific for phytate dephosphorylation. It was shown that the phytase preferably...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Phytate-degrading enzyme; Phytate; Phytase.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400010

Production of lytic enzymes by Trichoderma isolates during in vitro antagonism with Aspergillus niger, the causal agent of collar rot of peanut
Provedor de dados: 58
Autores: Gajera,H. P.; Vakharia,D. N.
Twelve isolates of Trichoderma (six of T. harzianum, five of T. viride, one of T. virens), which reduced variably the incidence of collar rot disease caused in peanut by Aspergillus niger Van Tieghem, were evaluated for their potential to produce lytic enzymes during in vitro antagonism. T. viride 60 inhibited highest (86.2%) growth of test fungus followed by T. harzianum 2J (80.4%) at 6 days after inoculation (DAI) on PDA media. The specific activities of chitinase, β-1,3-glucanase and protease were 11, 3.46 and 9 folds higher in T6 antagonist (T. viride 60 and A. niger interactions) followed by 8.72, 2.85 and 9 folds in T8 antagonist (T. harzianum 2J and A. niger interactions), respectively, compared to the activity produced by control petri plate T13...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichoderma isolates; Aspergillus niger; Lytic enzymes; Antagonistic interaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100005

Production of cellulase by Aspergillus niger under submerged and solid state fermentation using coir waste as a substrate
Provedor de dados: 58
Autores: Mrudula,Soma; Murugammal,Rangasamy.
Aspergillus niger was used for cellulase production in submerged (SmF) and solid state fermentation (SSF). The maximum production of cellulase was obtained after 72 h of incubation in SSF and 96 h in Smf. The CMCase and FPase activities recorded in SSF were 8.89 and 3.56 U per g of dry mycelial bran (DBM), respectively. Where as in Smf the CMase &amp;amp; FPase activities were found to be 3.29 and 2.3 U per ml culture broth, respectively. The productivity of extracellular cellulase in SSF was 14.6 fold higher than in SmF. The physical and nutritional parameters of fermentation like pH, temperature, substrate, carbon and nitrogen sources were optimized. The optimal conditions for maximum biosynthesis of cellulase by A. niger were shown to be at pH 6,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Coir waste; Cellulase; Submerged fermentation; Solid-state fermentation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300033

Selection of tannase-producing Aspergillus niger strains
Provedor de dados: 58
Autores: Pinto,Gustavo A.S.; Leite,Selma G.F.; Terzi,Selma C.; Couri,Sonia.
The aim of this work was to select strains of Aspergillus niger for tannase production. Growth of colonies in plates with tannic acid-containing medium indicated their ability to synthesize tannase. Tannase activity was also measured in solid-state fermentation. A. niger 11T25A5 was the best tannase producer (67.5 U.g-1/72 hours of fermentation).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tannase; Aspergillus niger; Screening solid-state; Fermentation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100006

Reversion by calcium of a yeast-like development to the original filamentous form, of the 10V10 5-fluorocytosine-sensitive mutant of Aspergillus niger
Provedor de dados: 58
Autores: Goulart,Rosangela de Carvalho; Marin,José Moacir.
Some filamentous fungi present the phenomenon of dimorphism, their morphological structure alterations being capable of inducing metabolism changes. The Aspergillus niger strain 10v10, a producer of citric acid, was submitted to the mutagenic action of ultraviolet irradiation which respectively selects mutants sensitive or resistant to the antifungi agent 5 fluorocytosine (5-FC). 5-FC sensitive mutants presented a morphological alteration to a yeast-like form. The effects of pH changes, addition of salts (KH2PO4, NH4NO3, MgSO4 and MgCl2), the presence of osmotic stabilizers, as well as of calcium chloride, on morphological reversal and acid production were studied. Morphological reversal to the filamentous form was observed only in the presence of CaCl2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; 5-fluorocytosine; Yeast-like; Calcium; Acid production; Dimorphism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300018

Optimizing culture conditions for production of intra and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 by response surface methodology (RSM)
Provedor de dados: 58
Autores: Dinarvand,Mojdeh; Rezaee,Malahat; Foroughi,Majid.
Abstract The aim of this study was obtain a model that maximizes growth and production of inulinase and invertase by Aspergillus niger ATCC 20611, employing response surface methodology (RSM). The RSM with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. Results showed that the experimental data could be appropriately fitted into a second-order polynomial model with a coefficient of determination (R2) more than 0.90 for all responses. This model adequately explained the data variation and represented the actual relationships between the parameters and responses. The pH and temperature value of the cultivation medium were the most significant variables and the effects of inoculum size and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intra-extracellular inulinase production; Intra-extracellular invertase production; Aspergillus niger; Optimization; Response surface method (RSM).
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300427

Improvement in nutritional quality of fibrous food via in vitro digestion by Aspergillus niger
Provedor de dados: 118
Autores: Velásquez,Alejandro; Marnet,Pierre-Guy; Arias,Rodrigo.
In a first experiment, the effect of in vitro incubation with Aspergillus niger (An) on the chemical composition of different fibrous substrates was studied. In a second experiment, the effect of incubation time (0,72 and 144 h) on in vitro digestion with An of dry matter (IVDMD), neutral detergent fiber (DVNDF) and acid detergent fiber (DVADF) was evaluated for different substrates. Wheat straw, WS; barley straw, BS; oat hulls, OH; dehydrated alfalfa, DA; and dehydrated ryegrass, DB substrates were evaluated. In both experiments, incubations were performed in 250 mL Erlenmeyer flasks; 2 g of substrate was added to a culture medium (pH = 6), and incubated at 28 °C with constant ventilation. The An dose consisted of 3 mL of a solution of 5.3 &amp;#967;...
Tipo: Journal article
Palavras-chave: Aspergillus niger; Fibrous substrates; In vitro digestion; Nutritional quality.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000100005

Activity of fungal phytases stored in two ways in response to the period of storage at room temperature
Provedor de dados: 65
Autores: Naves,Luciana de Paula; Corrêa,Angelita Duarte; Bertechini,Antônio Gilberto; Gomide,Elisangela Minati; Abreu,Celeste Maria Patto de.
Two distinct experiments were conducted simultaneously with phytases of Aspergillus oryzae and A. niger for determining enzyme activity in response to storage period (up to 180 days at room temperature - RT). In the first experiment, enzymes were stored as they were acquired (pure form) and the activity was measured periodically during 180 days of storage at RT. In the second experiment, the phytases were incorporated to a supplement containing vitamins, minerals, and amino acids; and then this supplement was stored at RT up to 180 days, so that every 30 days of storage was collected one aliquot from each replicate for determining enzymatic activity. In conclusion, the phytase activity is affected by storage duration. To ensure 80% of the initial activity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Aspergillus oryzae; Enzyme; Phytate; Phytic acid.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000100029

Aproveitamento de resíduos da agroindústria do óleo de dendê para a produção de lipase por Aspergillus Níger
Provedor de dados: 65
Autores: Penha,Edmar das Mercês; Viana,Ludmila de Araújo Nunes; Gottschalk,Leda Maria Fortes; Terzi,Selma da Costa; Souza,Erika Fraga de; Freitas,Sidinéa Cordeiro de; Santos,Juliana de Oliveira; Salum,Thaís Fabiana Chan.
RESUMO: Neste trabalho, foi avaliado o desempenho de duas linhagens de Aspergillus niger (mutante 11T53A14 e selvagem C) previamente selecionadas como promissoras para a produção de lipases, utilizando meios de cultivo formulados a partir da torta de dendê (palmiste) e da borra alcalina do refino do óleo de dendê (borra de dendê), resíduos provenientes da agroindústria do óleo de dendê (palma) por fermentação em estado sólido (FES). Os experimentos de produção da enzima em FES foram conduzidos em colunas aeradas, incubadas em banho-maria a 32ºC com entrada controlada de ar não umedecido de 1,0vvm. Os melhores resultados foram obtidos com a cepa mutante A. niger 11T53A14 em meio contendo torta de dendê umedecida com solução sulfato de amônio (1,2%) e com a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipase; Resíduo agroindustrial; Aspergillus niger.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400755

Optimization of productions of cellulolytic enzymes by Aspergillus niger using residue of mango a substrate
Provedor de dados: 65
Autores: Santos,Tamires Carvalho dos; Cavalcanti,Ingrid Souza; Bonomo,Renata Cristina Ferreira; Santana,Nivio Batista; Franco,Marcelo.
The present paper analyses the effects of water activity (0.88, 0.94 and 0.97) and of fermentation time (24, 48, 72, 96 and 120 hours) on the kinetic activity of enzymes cellulolytic, produced during the solid state fermentation of waste from the improvement of mango, with the aid of fungus species Aspergillus niger. Solid state fermentation was carried out at 35°C inside a bacteriological incubator. The statistical results indicated that the best activity for enzyme CMCase was 7.26U g-1 after 74.51 hours of fermentation, whereas for enzyme FPase was 2.55U g-1 after 98.52 hours, both presenting best results in approximately 0.928 of water activity. Pareto charts have showed that fermentation time has greater effect over the activity of enzyme CMCase, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Pareto charts; Solid state fermentation; Mangifera indica. L.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001200027