Consulta: subjectFacets:"Atherosclerosis"
Registros recuperados: 52
Data/hora: 06/06/2024 03:35:29

Combined GSTM1 and GSTT1 null genotypes are strong risk factors for atherogenesis in a Serbian population
Provedor de dados: 74
Autores: Grubisa,Ivana; Otasevic,Petar; Vucinic,Nada; Milicic,Biljana; Jozic,Tanja; Krstic,Slobodan; Milasin,Jelena.
Abstract Oxidative stress (OS) plays an important role in atherogenesis and since glutathione S-transferases (GSTs) provide protection against OS, we have tested the hypothesis that deletion polymorphisms in two GSTs (GSTM1 and GSTT1) may affect the risk of developing atherosclerosis. A total of 382 individuals (200 patients with atherosclerosis and 182 healthy controls) were included in this association study. Genomic DNA was isolated from peripheral blood cells or from buccal epithelial cells and genotyping was performed using multiplex-PCR or real-time PCR methods. GSTM1 null genotype was significantly more frequent in atherosclerotic patients than in controls (52.0% vs 34.1%) and individuals with the GSTM1 null genotype had an approximately 2-fold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Gene polymorphisms; Glutathione S-transferases; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100035

Matrix metalloproteinase gene polymorphisms in patients with coronary artery disease
Provedor de dados: 74
Autores: Dalepiane,Vanessa L.N.; Silvello,Daiane N.; Paludo,Crislaine A.; Roisenberg,Israel; Simon,Daniel.
Matrix metalloproteinases (MMPs) play an important role in the pathogenesis of atherosclerosis, the pathology underlying the majority of coronary artery disease (CAD). In this study we tested the hypothesis that polymorphic variation in the MMP genes influences the risk of developing atherosclerosis. We analyzed functional polymorphisms in the promoter of the MMP-1, MMP-3, MMP-9 and MMP-12 genes in 183 Brazilian Caucasian individuals submitted to coronary angiography, of which 67 (37%) had normal coronary arteries (control group) and 116 (63%) had CAD (CAD patient group). The -1607 1G/2G MMP-1, -1171 5A/6A MMP-3, -1562 C/T MMP-9, -82 A/G MMP-12 polymorphisms were analyzed by PCR followed by restriction digestion. No significant differences were observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Coronary artery disease; Gene polymorphisms; Matrix metalloproteinases.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400001

Plants considered useful for hypoglycemic, antihypertensive or hypolipidemic treatments by patients with peripheral vascular diseases
Provedor de dados: 98
Autores: García-Lazo,Gertrudis; Lauzant-Diaz,Ela; Díaz Batista,Arquímedes; Garcia-Mesa,Milagros.
Tipo: Journal article
Palavras-chave: Medicinal plant; Herb medicine; Diabetes mellitus; Arterial hypertension; Dyslipidemia; Glucose; Cholesterol; Atherosclerosis; Atherothrombosis.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962015000100004

Action of calcium binders.: Formation of calcium salts in the intestinal tract and the blood circulatory system
Provedor de dados: 38
Autores: Martínez Yaquelo,César.
Calcium binders are water- soluble substances whose anionic part can possibly react with calcium cations present in any part of the human body. Some examples of calcium binders are phosphoric, oxalic and phytic acids and their alkaline salts present in many natural foods, in some foods containing additives, and in some medicines. There are also uric and lactic acids that are the result of normal metabolism. The first negative action of calcium binders takes place in the intestinal tract when they react with calcium of dairy food. The latter produces a combination of calcium binder anions with calcium cations, and then an insoluble salt that precipitates as small solid particles that cannot be assimilated and which are eliminated in the faeces. The second...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium binders; Osteopenia; Osteoporosis; Atherosclerosis; Nephrosclerosis; Urolithyasis; Calcinosis; Vascular calcification.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572006000200008

Atheroprotective action of a modified organoselenium compound:  in vitro evidence
Provedor de dados: 42
Autores: OLIVEIRA,JADE DE; STRALIOTTO,MARCOS R.; MANCINI,GIANNI; FIGUEIREDO,CLAUDIA P.; BRAGA,ANTÔNIO L.; TEIXEIRA,JOÃO B.R.; BEM,ANDREZA F.
ABSTRACT Oxidation of low-density lipoprotein (LDL) has been strongly suggested to play a significant role in the pathogenesis of atherosclerosis. Thus, reducing LDL oxidation is a potential approach to decrease the risk of the atherosclerosis. Organoselenium compounds have demonstrated promising atheroprotective properties in experimental models. Herein, we tested the in vitro atheroprotective capability of a modified organoselenium compound, Compound HBD, in protecting isolated LDL from oxidation as well as foam cells formation. Moreover, the glutathione peroxidase (GPx)-like activity of Compound HBD was analyzed in order to explore the mechanisms related to the above-mentioned protective effects. The Compound HBD in a concentration-dependent manner...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Compound HBD; Foam cell; GPx-like activity; LDL oxidation; Organoselenium compounds.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501953

The role of cyclooxygenase-2 on endurance exercise training in female LDL-receptor knockout ovariectomized mice
Provedor de dados: 42
Autores: OLIVEIRA,FLAVIA DE; MAIFRINO,LAURA B.M.; JESUS,GUSTAVO P.P. DE; CARVALHO,JULIANA G.; MARCHON,CLÁUDIA; RIBEIRO,DANIEL A.
Estrogen deprivation in postmenopausal women increases cardiovascular risk. Cardiovascular risk as a result of atherosclerosis is able to induce an inflammatory disease as far as cyclooxygenase-2 ( COX-2) expression. The purpose of the study was to investigate the role of COX-2 on exercise training in female mice low-density lipoprotein receptor knockout ( LDL-KO) with or without ovariectomy. A total of 15 female C57BL/6 mice and 15 female LDL-KO mice were distributed into 6 groups: sedentary control, sedentary control ovariectomized, trained control ovariectomized, LDL-KO sedentary, LDL-KO sedentary ovariectomized and LDL-KO trained ovariectomized. The ascending part of the aorta was stained with H&amp;E and COX-2 expression was assessed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Ciclooxygenase-2; Exercise; Menopause.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301157

Alcohol and atherosclerosis
Provedor de dados: 42
Autores: DA LUZ,PROTASIO L.; COIMBRA,SILMARA R.
Atherosclerosis is manifested as coronary artery disease (CAD), ischemic stroke and peripheral vascular disease. Moderate alcohol consumption has been associated with reduction of CAD complications. Apparently, red wine offers more benefits than any other kind of drinks, probably due to flavonoids. Alcohol alters lipoproteins and the coagulation system. The flavonoids induce vascular relaxation by mechanisms that are both dependent and independent of nitric oxide, inhibits many of the cellular reactions associated with atherosclerosis and inflammation, such as endothelial expression of vascular adhesion molecules and release of cytokines from polymorphonuclear leukocytes. Hypertension is also influenced by the alcohol intake. Thus, heavy alcohol intake is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcohol; Coronary heart disease; Atherosclerosis; Stroke; Wine.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000100006

Modulatory effects of rutin on biochemical and hematological parameters in hypercholesterolemic Golden Syrian hamsters
Provedor de dados: 42
Autores: Kanashiro,Alexandre; Andrade,Daiani C.O.; Kabeya,Luciana M.; Turato,Walter M.; Faccioli,Lucia H.; Uyemura,Sérgio A.; Lucisano-Valim,Yara M.
Flavonoids have been reported to exhibit several pharmacological properties, mainly in cardiovascular and inflammatory diseases. In the present study, we observed that rutin, a known glycosylated flavonoid isolated from Dimorphandra mollis, had a lowering effect on plasma triglyceride levels of diet-induced hypercholesterolemic Golden Syrian hamsters, but did not change total cholesterol and high-density lipoprotein cholesterol levels. Moreover, high-fat or rutin supplemented diets showed no immunotoxic effects, since no significant changes were observed on total white blood cells, granulocytes and mononuclear cells, as well as on the neutrophil apoptosis degree, when compared to untreated animals. Therefore, rutin seems to be a selective and non-toxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rutin; Golden Syrian hamster; Hypercholesterolemia; Atherosclerosis; Apoptosis; Dimorphandra mollis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000100009

Red Wine administration to Apolipoprotein E-deficient Mice reduces their Macrophage-derived Extracellular Matrix Atherogenic Properties
Provedor de dados: 48
Autores: KAPLAN,MARIELLE; AVIRAM,MICHAEL.
Proteoglycans (PGs) from the arterial extracellular matrix (ECM) contribute to the trapping of LDL and oxidized LDL (Ox-LDL) in the arterial wall, a phenomenon called "lipoprotein retention". Moreover, we have shown that subsequent to their binding to the matrix, LDL and Ox-LDL are taken up by macrophages. Oxidative stress significantly increases macrophage secretion of ECM-PGs, lipoprotein binding to the ECM and the uptake of ECM-retained lipoproteins by macrophages. The aim of the present study was to determine whether red wine administration to atherosclerotic mice would affect their peritoneal macrophage-derived extracellular matrix properties, such as the glycosaminoglycan content and the ability to bind LDL. In addition, we questioned the ability of...
Tipo: Journal article
Palavras-chave: Antioxidants; Atherosclerosis; Extracellular matrix; Macrophages; Polyphenols; Proteoglycans; Red wine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200010

Endothelial cell oxidative stress and signal transduction
Provedor de dados: 48
Autores: FONCEA,ROCIO; CARVAJAL,CRISTIAN; ALMARZA,CAROLINA; LEIGHTON,FEDERICO.
Endothelial dysfunction (ED) is an early event in atherosclerotic disease, preceding clinical manifestations and complications. Increased reactive oxygen species (ROS) have been implicated as important mechanisms that contribute to ED, and ROS’s may function as intracellular messengers that modulate signaling pathways. Several intracellular signal events stimulated by ROS have been defined, including the identification of two members of the mitogen activated protein kinase family (ERK1/2 and big MAP kinase, BMK1), tyrosine kinases (Src and Syk) and different isoenzymes of PKC as redox-sensitive kinases. ROS regulation of signal transduction components include the modification in the activity of transcriptional factors such as NFkB and others that result in...
Tipo: Journal article
Palavras-chave: Oxidative stress; Signal transduction; Gene expression; Endothelial cell; Atherosclerosis; Antioxidants.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200008

Lipid peroxidation and antioxidants in hyperlipidemia and hypertension
Provedor de dados: 48
Autores: MORIEL,PATRICIA; PLAVNIK,FRIDA L; ZANELLA,MARIA T; BERTOLAMI,MARCELO C; ABDALLA,DULCINEIA SP.
Lipid peroxidation and lipid-derived oxidized products have been implicated in the pathogenesis of a variety of human diseases. To clarify the role of oxidative stress in essential hypertension and hypercholesterolemia the in vitro oxidative susceptibility of LDL, the antioxidant status and the lipid peroxide content of blood plasma were examined in hypercholesterolemic (HC), hypertensive (H), hypercholesterolemic/hypertensive (HH) and normolipidemic/normotensive subjects (N). Plasma ascorbate and lipid-soluble antioxidants were lower, while LDL oxidizability, CE-OOH and TL-OOH were higher in H, HC, and HH groups than in the N group. No difference was observed among groups for PL-OOH and isoprostanes. In summary, the results show that: 1) lipid- and...
Tipo: Journal article
Palavras-chave: Lipoprotein oxidation; Lipid peroxides; Isoprostanes; Antioxidants; Atherosclerosis; Oxidative stress.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200010

MnSOD and GPx1 polymorphism relationship with coronary heart disease risk and severity
Provedor de dados: 48
Autores: Souiden,Yosra; Mallouli,Hela; Meskhi,Salah; Chaabouni,Yassine; Rebai,Ahmed; Chéour,Foued; Mahdouani,Kacem.
BACKGROUND: Disturbance of the equilibrium between reactive oxygen species (ROS) and anti-oxidants (AOX) has been implicated in various diseases, including atherosclerosis, the most common pathologic process underlying coronary heart disease (CHD). Thus, the defense systems against ROS are critical protecting blood vessel walls against oxidative damage. In this study, we investigate whether Ala16Val MnSOD and Pro198Leu GPx polymorphisms are associated with CHD susceptibility and/or severity METHODS: Both polymorphisms were genotyped in a sample of 203 controls and 164 patients. CHD risk and severity, antioxidant status (enzymatic and/or non enzymatic) and biochemical parameters were assessed and analysed by genotype RESULTS: A significant association of...
Tipo: Journal article
Palavras-chave: Genetic polymorphism; Coronary heart disease; SOD activity; GPx activity; Total antioxidant status; Atherosclerosis.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100022

Scavenger Receptors and Atherosclerosis
Provedor de dados: 48
Autores: RIGOTTI,ATTILIO.
Scavenger receptors were discovered as cell surface proteins capable of binding and internalization of modified lipoproteins. These receptors exhibit a broad ligand binding specificity including potential physiological and pathophysiological ligands other than modified lipoproteins. Different classes of scavenger receptors have been identified, and their relevance in normal and pathological conditions is under investigation. Recent in vitro and in vivo studies strongly support the role of class A and class B scavenger receptors in lipid transport atherogenesis
Tipo: Journal article
Palavras-chave: Atherosclerosis; Cholesterol metabolism; Lipoprotein; Scavenger receptor; Selective cholesterol uptake.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200009

Comparison of apolipoprotein E polymorphism and other atherosclerotic risk factors in two portuguese populations: the influence of insularity
Provedor de dados: 52
Autores: Lopes,Paula Alexandra; Gaspar,Gisela; Napoleão,Patrícia; Santos,Maria Cristina; Pavão,Maria Leonor; Viegas-Crespo,Ana Maria.
The main objective of this study was to investigate the apolipoprotein (apo) E genotypes in Portuguese populations from mainland (Lisbon city) and from San Miguel Island, Azores' Archipelago (Ponta Delgada city) and to look for differences between these particular sites in apparently healthy subjects. Also, subjects with clinical diagnosis of atherosclerotic disease were investigated in San Miguel Island. In Lisbon, the genotypes distribution was: epsilon3/epsilon 3 &amp;gt; epsilon 3/epsilon 4 &amp;gt; epsilon 2/epsilon 3 &amp;gt; epsilon 4/epsilon 4 while that, for Ponta Delgada and regardless the health condition, was: epsilon 3/epsilon 3 &amp;gt; epsilon 2/epsilon 3 &amp;gt; epsilon 3/epsilon 4. Within Ponta Delgada control group, females and males had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E polymorphism; Cardiovascular risk factors; Atherosclerosis; Insularity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500008

Lipid accumulation product index in HIV-infected patients: a marker of cardiovascular risk
Provedor de dados: 55
Autores: Guimarães,Milena Maria Moreira; Greco,Dirceu Bartolomeu; Moreira,Allyson Nogueira; Guimarães,Nathalia Sernizon; Freire,Cláudia Maria Vilas; Rohlfs,Bruna Guimarães; Machado,Lucas José de Campos.
ABSTRACT The lipid accumulation product (LAP) index is an emerging cardiovascular risk marker. We aimed to assess the accuracy of this index as a marker of cardiovascular risk in HIV-infected patients. A cross-sectional study of 133 HIV-infected patients on antiretroviral drugs and 20 non-infected controls was conducted at the outpatient clinic of a referral center of infectious and parasitic diseases. Evaluations included LAP index, homeostasis model assessment (HOMA) index, anthropometric measurements, blood pressure, glucose tolerance test, and cholesterol and triglyceride levels. Body mass index (BMI) was similar in both groups; however, waist circumference was greater in the HIV-infected patients. Triglyceride levels were significantly higher (p &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Hyperlipidemia; Atherosclerosis; Lipid accumulation product index; Cardiovascular risk; Insulin resistance.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300171

Endothelial function and cardiovascular diseases in HIV infected patient
Provedor de dados: 55
Autores: Andrade,Ana Cristina O.; Cotter,Bruno R.
The HIV epidemic has dramatically changed the paradigm for the development of drug therapy in the last 15 years. The goal is now not only to provide an effective reduction of plasma viremia , but also to reconstitute the immune deficiency due to the progression of the disease. Significant problems with the metabolism of sugars and lipids lead to the appearance of well-documented disorders such as insulin resistance, abnormalities in lipid metabolism and lipodystrophy in those patients on prolonged therapy with antiretrovirals. The question of whether or not HAART-associated lipid disorders contribute to the premature development of coronary artery disease is of major importance for the HIV community. Endothelial injury is associated with disease-related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human immunodeficiency virus; Endothelial dysfunction; Endothelial adhesion molecules; Antiretroviral therapy; Cardiovascular risk factors; Atherosclerosis; Coronary heart disease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000200012

Hyperhomocystinemia in patients with coronary artery disease
Provedor de dados: 56
Autores: Faria-Neto,J.R.; Chagas,A.C.P.; Bydlowski,S.P.; Lemos Neto,P.A.; Chamone,D.A.; Ramirez,J.A.F.; da Luz,P.L.
Hyperhomocystinemia has been related to an increased risk of cardiovascular disease in several studies. The C677T polymorphism for the gene that encodes the methylenetetrahydrofolate reductase enzyme (MTHFR) and low plasma folate levels are common causes of hyperhomocystinemia. Due to differences in nutritional patterns and genetic background among different countries, we evaluated the role of hyperhomocystinemia as a coronary artery disease (CAD) risk factor in a Brazilian population. The relation between homocysteine (Hcy) and the extent of CAD, measured by an angiographic score, was determined. A total of 236 patients referred for coronary angiography for clinical reasons were included. CAD was found in 148 (62.7%) patients and 88 subjects had normal or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperhomocystinemia; Homocysteine; Methylenetetrahydrofolate reductase; Atherosclerosis; Folic acid deficiency.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400005

Plasma clearance and biodistribution of oxidatively modified 99mTc-ß-VLDL in rabbits
Provedor de dados: 56
The biodistribution and removal from plasma (measured as fractional clearance rate, FCR, per hour) of native and oxidatively modified 99mtechnetium-labeled ß-very low density lipoprotein (99mTc-ß-VLDL) were investigated in hypercholesterolemic (HC) and control (C) three-month old New Zealand rabbits. The intracellular accumulation of ß-VLDL labeled with 99mTc was studied in vitro in THP-1 cells and monocyte-derived macrophages isolated from rabbits. After intravenous injection into C rabbits, copper-oxidized ß-VLDL (99mTc-ox-ß-VLDL) was cleared from the circulation faster (0.362 ± 0.070/h) than native ß-VLDL (99mTc-nat-ß-VLDL, 0.241 ± 0.070/h). In contrast, the FCR of 99mTc-ox-ß-VLDL in HC rabbits was lower (0.100 ± 0.048/h) than that of 99mTc-nat-ß-VLDL...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: SS-very low density lipoprotein; Copper-mediated oxidation; Biodistribution; Fractional clearance rate; Atherosclerosis; Hypercholesterolemia; Cholesterol feeding; Rabbit.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600002

Effect of Lactobacillus delbrueckii on cholesterol metabolism in germ-free mice and on atherogenesis in apolipoprotein E knock-out mice
Provedor de dados: 56
Autores: Portugal,L.R.; Gonçalves,J.L.; Fernandes,L.R.; Silva,H.P.S.; Arantes,R.M.E.; Nicoli,J.R.; Vieira,L.Q.; Alvarez-Leite,J.I.
Elevated blood cholesterol is an important risk factor associated with atherosclerosis and coronary heart disease. Several studies have reported a decrease in serum cholesterol during the consumption of large doses of fermented dairy products or lactobacillus strains. The proposed mechanism for this effect is the removal or assimilation of intestinal cholesterol by the bacteria, reducing cholesterol absorption. Although this effect was demonstrated in vitro, its relevance in vivo is still controversial. Furthermore, few studies have investigated the role of lactobacilli in atherogenesis. The aim of the present study was to determine the effect of Lactobacillus delbrueckii on cholesterol metabolism in germ-free mice and the possible hypocholesterolemic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus; Atherosclerosis; Apolipoprotein E; Cholesterol; Germ-free mice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500010

Correlation between serum 25-hydroxyvitamin D levels and carotid intima-media thickness in a Brazilian population descended from African slaves
Provedor de dados: 56
Autores: Monteiro Júnior,F.C.; Mandarino,N.R.; Santos,E.M.; Santos,A.M.; Salgado,J.V.; Brito,D.J.A.; Salgado,B.J.L.; Lages,J.S.; Castelo Branco,G.; Salgado Filho,N.
Hypovitaminosis D has been identified as a possible new cardiovascular risk factor. However, the results of studies correlating serum vitamin D levels with markers of subclinical atherosclerosis have been conflicting. The aim of this study was to correlate serum levels of 25-hydroxyvitamin D [25(OH)D] with carotid intima-media thickness (C-IMT) and conventional cardiovascular risk factors in Afro-descendants. A cross-sectional analysis was performed on a sample of 382 individuals from a cohort of descendants of African slaves, inhabitants of “Quilombola” communities, with a mean age of 57.79 ±15.3 years, 54.5% of whom were women. Socio-demographic and clinical data were collected and biochemical tests were performed, including serum levels of 25(OH)D by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamin D deficiency; Carotid intima-media thickness (C-IMT); Atherosclerosis; African continental ancestry group.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400606

Side effects of oxysterols: cytotoxicity, oxidation, inflammation, and phospholipidosis
Provedor de dados: 56
Autores: Vejux,A.; Malvitte,L.; Lizard,G.
Oxysterols are 27-carbon atom molecules resulting from autoxidation or enzymatic oxidation of cholesterol. They are present in numerous foodstuffs and have been demonstrated to be present at increased levels in the plasma of patients with cardiovascular diseases and in atherosclerotic lesions. Thus, their role in lipid disorders is widely suspected, and they might also be involved in important degenerative diseases such as Alzheimer's disease, osteoporosis, and age-related macular degeneration. Since atherosclerosis is associated with the presence of apoptotic cells and with oxidative and inflammatory processes, the ability of some oxysterols, especially 7-ketocholesterol and 7β-hydroxycholesterol, to trigger cell death, activate inflammation, and modulate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Atherosclerosis; Inflammation; Oxysterols; Phospholipidosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700001

Structural basis for the pathophysiology of lipoprotein(a) in the athero-thrombotic process
Provedor de dados: 56
Autores: Anglés-Cano,E.
Lipoprotein Lp(a) is a major and independent genetic risk factor for atherosclerosis and cardiovascular disease. The essential difference between Lp(a) and low density lipoproteins (LDL) is apolipoprotein apo(a), a glycoprotein structurally similar to plasminogen, the precursor of plasmin, the fibrinolytic enzyme. This structural homology endows Lp(a) with the capacity to bind to fibrin and to membrane proteins of endothelial cells and monocytes, and thereby to inhibit plasminogen binding and plasmin generation. The inhibition of plasmin generation and the accumulation of Lp(a) on the surface of fibrin and cell membranes favor fibrin and cholesterol deposition at sites of vascular injury. Moreover, insufficient activation of TGF-ß due to low plasmin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Apolipoprotein(a); Thrombosis; Atheroma; Plasminogen; Lipoprotein; Fibrin; Fibrinolysis.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100002

Neuronal and endothelial nitric oxide synthase gene knockout mice
Provedor de dados: 56
Autores: Huang,P.L.
Targeted disruption of the neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS) genes has led to knockout mice that lack these isoforms. These animal models have been useful to study the roles of nitric oxide (NO) in physiologic processes. nNOS knockout mice have enlarged stomachs and defects in the inhibitory junction potential involved in gastrointestinal motility. eNOS knockout mice are hypertensive and lack endothelium-derived relaxing factor activity. When these animals are subjected to models of focal ischemia, the nNOS mutant mice develop smaller infarcts, consistent with a role for nNOS in neurotoxicity following cerebral ischemia. In contrast, eNOS mutant mice develop larger infarcts, and show a more pronounced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Targeted disruption; Gene knockout; Endothelium; Stroke; Atherosclerosis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100005

Drugs and lifestyle for the treatment and prevention of coronary artery disease: comparative analysis of the scientific basis
Provedor de dados: 56
Autores: da Luz,P.L.; Nishiyama,M.; Chagas,A.C.P.
In this article, we compare two strategies for atherosclerosis treatment: drugs and healthy lifestyle. Statins are the principal drugs used for the treatment of atherosclerosis. Several secondary prevention studies have demonstrated that statins can significantly reduce cardiovascular events including coronary death, the need for surgical revascularization, stroke, total mortality, as well as fatal and non-fatal myocardial infarction. These results were observed in both men and women, the elderly, smokers and non-smokers, diabetics and hypertensives. Primary prevention studies yielded similar results, although total mortality was not affected. Statins also induce atheroma regression and do not cause cancer. However, many unresolved issues remain, such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Statins; Lifestyle; Coronary disease; Prevention.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000002

Atherosclerosis in aged mice over-expressing the reverse cholesterol transport genes
Provedor de dados: 56
Autores: Berti,J.A.; de Faria,E.C.; Oliveira,H.C.F.
We determined whether over-expression of one of the three genes involved in reverse cholesterol transport, apolipoprotein (apo) AI, lecithin-cholesterol acyl transferase (LCAT) and cholesteryl ester transfer protein (CETP), or of their combinations influenced the development of diet-induced atherosclerosis. Eight genotypic groups of mice were studied (AI, LCAT, CETP, LCAT/AI, CETP/AI, LCAT/CETP, LCAT/AI/CETP, and non-transgenic) after four months on an atherogenic diet. The extent of atherosclerosis was assessed by morphometric analysis of lipid-stained areas in the aortic roots. The relative influence (R²) of genotype, sex, total cholesterol, and its main sub-fraction levels on atherosclerotic lesion size was determined by multiple linear regression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein AI; Cholesteryl ester transfer protein; Lecithin-cholesterol acyl transferase; Atherosclerosis; Transgenic mice.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300010

Polymorphisms of the low-density lipoprotein receptor gene in Brazilian individuals with heterozygous familial hypercholesterolemia
Provedor de dados: 56
Autores: Salazar,L.A.; Cavalli,S.A.; Hirata,M.H.; Diament,J.; Forti,N.; Giannini,S.D.; Nakandakare,E.R.; Bertolami,M.C.; Hirata,R.D.C.
Familial hypercholesterolemia (FH) is a metabolic disorder inherited as an autosomal dominant trait characterized by an increased plasma low-density lipoprotein (LDL) level. The disease is caused by several different mutations in the LDL receptor gene. Although early identification of individuals carrying the defective gene could be useful in reducing the risk of atherosclerosis and myocardial infarction, the techniques available for determining the number of the functional LDL receptor molecules are difficult to carry out and expensive. Polymorphisms associated with this gene may be used for unequivocal diagnosis of FH in several populations. The aim of our study was to evaluate the genotype distribution and relative allele frequencies of three...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Familial hypercholesterolemia; DNA polymorphism; Atherosclerosis; Genetics; LDL receptor.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100006

Influences of alpha-tocopherol on cholesterol metabolism and fatty streak development in apolipoprotein E-deficient mice fed an atherogenic diet
Provedor de dados: 56
Autores: Peluzio,M.C.G.; Homem,A.P.P.; Cesar,G.C.; Azevedo,G.S.; Amorim,R.; Cara,D.C.; Saliba,H.; Vieira,E.C.; Arantes,R.E.; Alvarez-Leite,J.
Although the role of oxidized lipoproteins is well known in atherogenesis, the role of vitamin E supplementation is still controversial. There is also little information about cholesterol metabolism (hepatic concentration and fecal excretion) in the new models of atherosclerosis. In the present study, we evaluated the effect of moderate vitamin E supplementation on cholesterol metabolism and atherogenesis in apolipoprotein E (apo E)-deficient mice. Apo E-deficient mice were fed an atherogenic diet containing 40 or 400 mg/kg of alpha-tocopherol acetate for 6 weeks. Total cholesterol in serum and liver and 3-OH-alpha-sterols in feces, and fecal excretion of bile acids were determined and histological analyses of aortic lesion were performed. A vitamin E-rich...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Atherosclerosis; Apo E; Mice; Alpha-tocopherol; Cholesterol.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200005

Zymosan phagocytosis by mouse peritoneal macrophages is increased by apoHDL- and not by intact HDL-covered particles
Provedor de dados: 56
Autores: Carvalho,M.D.T.; Tobias,V.E.; Vendrame,C.M.V.; Shimabukuro,A.F.M.; Gidlund,M.; Quintão,E.C.R.
The uptake of lipids and lipoprotein particles by macrophages undergoes phagocytic activation and the formation of foam cells are key events in atherosclerosis. In this study we determined how intact high density lipoproteins (HDL) and apolipoproteins-HDL (removal of the lipid component from HDL, i.e., apoHDL) influence the phagocytosis of zymosan by mouse peritoneal macrophages. Zymosan particles preincubated together with lipoproteins or alone (control) were incubated with the macrophages. Phagocytosis activity was reported as the percent of macrophages that internalized three or more zymosan particles. HDL co-incubated with zymosan did not influence the over-all uptake of zymosan particles compared to apoHDL, which greatly enhanced the ability of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HDL; ApoHDL; Zymosan particles; Atherosclerosis; Phagocytosis; Macrophages.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300009

Coronary artery plaque burden and calcium scores in healthy men adhering to long-term wine drinking or alcohol abstinence
Provedor de dados: 56
Autores: da Luz,P.L.; Coimbra,S.; Favarato,D.; Albuquerque,C.; Mochiduky,R.I.; Rochitte,C.E.; Hojaij,E.; Gonsalves,C.R.L.; Laurindo,F.R.
Observational studies suggest there are clinical benefits to moderate red wine (RW) consumption. However, the effects on coronary vasculature and overall lifestyle are unclear. We investigated whether a lifestyle of regular long-term RW consumption is associated with changes in coronary plaque burden, calcium score, carotid intima/media thickness, endothelial function, and metabolic variables, compared with alcohol abstinence. Healthy volunteers were evaluated by coronary computed tomography angiography (CTA) as well as carotid and brachial artery ultrasound. Nutritional status, psychological status, and metabolic variables were assessed. The study included 101 drinkers [aged 58.9±7.3 years (means±SD)], from wine brotherhoods, and 104 abstainers, from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Red wine; Lifestyle; Computer tomography angiography; Coronary calcium; Carotid plaques.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800697

Bone metabolism and vascular calcification
Provedor de dados: 56
Autores: Danilevicius,C.F.; Lopes,J.B.; Pereira,R.M.R.
Osteoporosis and atherosclerosis are chronic degenerative diseases which have been considered to be independent and whose common characteristic is increasing incidence with age. At present, growing evidence indicates the existence of a correlation between cardiovascular disease and osteoporosis, irrespective of age. The morbidity and mortality of osteoporosis is mainly related to the occurrence of fractures. Atherosclerosis shows a high rate of morbidity and especially mortality because of its clinical repercussions such as angina pectoris, acute myocardial infarction, stroke, and peripheral vascular insufficiency. Atherosclerotic disease is characterized by the accumulation of lipid material in the arterial wall resulting from autoimmune and inflammatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Vascular calcification; Atherosclerosis; Low-bone mineral density; Arterial calcification.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400001

Lipoic acid, but not tempol, preserves vascular compliance and decreases medial calcification in a model of elastocalcinosis
Provedor de dados: 56
Autores: Bassi,E.; Liberman,M.; Martinatti,M.K.; Bortolotto,L.A.; Laurindo,F.R.M.
Vascular calcification decreases compliance and increases morbidity. Mechanisms of this process are unclear. The role of oxidative stress and effects of antioxidants have been poorly explored. We investigated effects of the antioxidants lipoic acid (LA) and tempol in a model of atherosclerosis associated with elastocalcinosis. Male New Zealand white rabbits (2.5-3.0 kg) were fed regular chow (controls) or a 0.5% cholesterol (chol) diet+104 IU/day vitamin D2 (vitD) for 12 weeks, and assigned to treatment with water (vehicle, n=20), 0.12 mmol·kg-1·day-1 LA (n=11) or 0.1 mmol·kg-1·day-1 tempol (n=15). Chol+vitD-fed rabbits developed atherosclerotic plaques associated with expansive remodeling, elastic fiber disruption, medial calcification, and increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Vascular calcification; Atherosclerosis; Oxidative stress; Lipoic acid.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200119

Lack of a relationship between serum ferritin levels and coronary atherosclerosis evaluated by coronary arteriography
Provedor de dados: 56
Autores: Manfroi,W.C.; Zago,A.J.; Cruz,R.; Oliveira,J.; Kirschnick,L.S.; Ordovás,K.; Candiago,R.H.; Souza,J.; Ribeiro,L.W.; Leitão,C.; Brizolara,M.L.
Many clinical and epidemiological studies have demonstrated the relationship between serum ferritin and ischemic heart disease. In the present study we evaluated the relationship between coronary heart disease (CHD) and serum ferritin levels in patients submitted to coronary arteriography. We evaluated 307 patients (210 (68.7%) males; median age: 60 years) who were submitted to coronary angiography, measurement of serum ferritin and identification of clinical events of ischemic heart disease. Serum ferritin is reported as quartiles. Ninety-six patients (31.27%) had normal coronary angiography (group 1) and 211 (68.73%) had coronary heart disease (group 2). Of the patients with CHD, 61 (28.9%) had serum ferritin levels higher than 194 ng/ml (4th quartile),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ferritin; Coronary heart disease; Atherosclerosis; Risk factors; Coronary arteriography.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300009

Role of chemokines in promoting instability of coronary atherosclerotic plaques and the underlying molecular mechanism
Provedor de dados: 56
Autores: Zhong,Z.X.; Li,B.; Li,C.R.; Zhang,Q.F.; Liu,Z.D.; Zhang,P.F.; Gu,X.F.; Luo,H.; Li,M.J.; Luo,H.S.; Ye,G.H.; Wen,F.L.
Our aim was to investigate the role of chemokines in promoting instability of coronary atherosclerotic plaques and the underlying molecular mechanism. Coronary angiography and intravascular ultrasound (IVUS) were performed in 60 stable angina pectoris (SAP) patients and 60 unstable angina pectoris (UAP) patients. The chemotactic activity of monocytes in the 2 groups of patients was examined in Transwell chambers. High-sensitivity C-reactive protein (hs-CRP), monocyte chemoattractant protein-1 (MCP-1), regulated on activation in normal T-cell expressed and secreted (RANTES), and fractalkine in serum were examined with ELISA kits, and expression of MCP-1, RANTES, and fractalkine mRNA was examined with real-time PCR. In the SAP group, 92 plaques were detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Chemokine; Plaque; MCP-1; RANTES.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200161

Th1 polarized response induced by intramuscular DNA-HSP65 immunization is preserved in experimental atherosclerosis
Provedor de dados: 56
Autores: Fonseca,D.M.; Bonato,V.L.D.; Silva,C.L.; Sartori,A.
We previously reported that a DNA vaccine constructed with the heat shock protein (HSP65) gene from Mycobacterium leprae (DNA-HSP65) was protective and also therapeutic in experimental tuberculosis. By the intramuscular route, this vaccine elicited a predominant Th1 response that was consistent with its protective efficacy against tuberculosis. It has been suggested that the immune response to Hsp60/65 may be the link between exposure to microorganisms and increased cardiovascular risk. Additionally, the high cholesterol levels found in atherosclerosis could modulate host immunity. In this context, we evaluated if an atherogenic diet could modulate the immune response induced by the DNA-HSP65 vaccine. C57BL/6 mice (4-6 animals per group) were initially...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA-HSP65; Genetic vaccine; Atherosclerosis; Tuberculosis; Th1; Cholesterol.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100010

Circulating levels of adiponectin and extent of coronary artery disease in patients undergoing elective coronary angiography
Provedor de dados: 56
Autores: Souza,R.A.; Alves,C.M.R.; de Oliveira,C.S.V.; Reis,A.F.; Carvalho,A.C.
Adiponectin (APN), an adipose tissue-released adipokine with demonstrated anti-inflammatory and anti-atherogenic properties, is encoded by a gene whose polymorphisms are associated with presence of coronary artery disease (CAD). Serum APN levels are inversely related with presence and complexity of CAD. Within this context, we sought to compare levels of total APN and its high molecular weight form (HMW APN) according to clinical presentation and extent of CAD in patients undergoing elective cardiac catheterization. From March 2008 to June 2010, clinical data and blood samples for APN and HMW APN measurements were collected from 415 subjects undergoing cardiac catheterization at two tertiary centers. CAD extent was estimated by the number of coronary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipose tissue; Adipokines; Adiponectin; Coronary artery disease; Atherosclerosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200604

Apolipoprotein E polymorphism in Brazilian dyslipidemic individuals: Ouro Preto study
Provedor de dados: 56
Autores: Mendes-Lana,A.; Pena,G.G.; Freitas,S.N.; Lima,A.A.; Nicolato,R.L.C.; Nascimento-Neto,R.M.; Machado-Coelho,G.L.L.; Freitas,R.N.
The influence of apolipoprotein E alleles and genotypes on plasma lipid levels was determined in 185 individuals of mixed ethnicity living in Ouro Preto, Brazil. DNA was obtained from blood samples and the genotypes were determined by an RFLP-PCR procedure. The *3 allele was the most frequent (72%), followed by *4 (20%) and *2 (8%); *4 frequency was higher and *2 frequency was lower in the dyslipidemic group than in the normal control group. The *2 carriers presented lower LDL and total cholesterol levels compared to the *3 and *4 carriers. All six expected genotypes were observed in the individuals genotyped: E2/2 (2.1%), E4/4 (2.7%), E2/4 (3.7%), E2/3 (8.0%), E3/3 (53.3%), E3/4 (29.9%); no difference in genotype frequencies was found between the normal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E polymorphism; Lipids; Dyslipidemia; Brazilian population; Atherosclerosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100007

Oral rapamycin attenuates atherosclerosis without affecting the arterial responsiveness of resistance vessels in apolipoprotein E-deficient mice
Provedor de dados: 56
Autores: Gadioli,A.L.N.; Nogueira,B.V.; Arruda,R.M.P.; Pereira,R.B.; Meyrelles,S.S.; Arruda,J.A.; Vasquez,E.C.
The objective of the present study was to assess the effects of the immunosuppressant rapamycin (Rapamune®, Sirolimus) on both resistance vessel responsiveness and atherosclerosis in apolipoprotein E-deficient 8-week-old male mice fed a normal rodent diet. Norepinephrine (NE)-induced vasoconstriction, acetylcholine (ACh)- and sodium nitroprusside (SNP)-induced vasorelaxation of isolated mesenteric bed, and atherosclerotic lesions were evaluated. After 12 weeks of orally administered rapamycin (5 mg·kg-1·day-1, N = 9) and compared with untreated (control, N = 9) animals, rapamycin treatment did not modify either NE-induced vasoconstriction (maximal response: 114 ± 4 vs 124 ± 10 mmHg, respectively) or ACh- (maximal response: 51 ± 8 vs 53 ± 5%, respectively)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rapamycin; Sirolimus; Atherosclerosis; Vascular responsiveness; Apolipoprotein E; Mice.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200012

Up-regulated miR-106b inhibits ox-LDL-induced endothelial cell apoptosis in atherosclerosis
Provedor de dados: 56
Autores: Zhang,Yunqing; Wang,Li; Xu,Jie; Kong,Xiaomei; Zou,Lin.
This research aimed to explore the molecular mechanism of microRNA (miR)-106b in cell apoptosis of atherosclerosis (AS). Human aortic endothelial cells (HAECs) were divided into control group, oxidized-low-density lipoproteins (ox-LDL) group, miR-106b NC+ox-LDL group, miR-106b mimics+ox-LDL group, miR-106b mimics+PTEN+ox-LDL group, and miR-106b mimics+empty+ox-LDL group. Real-time fluorescence quantitative polymerase chain reaction, cholecystokinin, TdT-mediated biotinylated nick end-labeling assay, luciferase reporter gene assay, and flow cytometry analysis were performed to determine the morphology, proliferation, and apoptosis in HSECs. Moreover, the levels of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), Bcl-2, p-P13K, and p-AKT in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Ox-LDL; MiR-106b; PTEN; P13K/AKT; Endothelial cell apoptosis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300610

Different methods of calculating ankle-brachial index in mid-elderly men and women: the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)
Provedor de dados: 56
Autores: Miname,M.; Bensenor,I.M.; Lotufo,P.A.
The ankle-brachial index (ABI) is a marker of subclinical atherosclerosis related to health-adverse outcomes. ABI is inexpensive compared to other indexes, such as coronary calcium score and determination of carotid artery intima-media thickness (IMT). Our objective was to identify how the ABI can be applied to primary care. Three different methods of calculating the ABI were compared among 13,921 men and women aged 35 to 74 years who were free of cardiovascular diseases and enrolled in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). The ABI ratio had the same denominator for the three categories created (the highest value for arm systolic blood pressure), and the numerator was based on the four readings for leg systolic blood pressure: the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peripheral artery disease; Risk factors; Atherosclerosis; Cardiovascular risk; Primary care.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200701

Elevated levels of plasma osteoprotegerin are associated with all-cause mortality risk and atherosclerosis in patients with stages 3 to 5 chronic kidney disease
Provedor de dados: 56
Autores: Nascimento,M.M.; Hayashi,S.Y.; Riella,M.C.; Lindholm,B.
Osteoprotegerin (OPG) regulates bone mass by inhibiting osteoclast differentiation and activation, and plays a role in vascular calcification. We evaluated the relationship between osteoprotegerin levels and inflammatory markers, atherosclerosis, and mortality in patients with stages 3-5 chronic kidney disease. A total of 145 subjects (median age 61 years, 61% men; 36 patients on hemodialysis, 55 patients on peritoneal dialysis, and 54 patients with stages 3-5 chronic kidney disease) were studied. Clinical characteristics, markers of mineral metabolism (including fibroblast growth factor-23 [FGF-23]) and inflammation (high-sensitivity C-reactive protein [hsCRP] and interleukin-6 [IL-6]), and the intima-media thickness (IMT) in the common carotid arteries...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Chronic kidney disease; Inflammation; Mortality; Osteoprotegerin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100995

An update on the tools for creating transgenic animal models of human diseases – focus on atherosclerosis
Provedor de dados: 56
Autores: Volobueva,A.S.; Orekhov,A.N.; Deykin,A.V.
Animal models of diseases are invaluable tools of modern medicine. More than forty years have passed since the first successful experiments and the spectrum of available models, as well as the list of methods for creating them, have expanded dramatically. The major step forward in creating specific disease models was the development of gene editing techniques, which allowed for targeted modification of the animal's genome. In this review, we discuss the available tools for creating transgenic animal models, such as transgenesis methods, recombinases, and nucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and CRISPR/Cas9 systems. We then focus specifically on the models of atherosclerosis, especially...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal models; Gene editing; Atherosclerosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500301

HFE gene mutations in coronary atherothrombotic disease
Provedor de dados: 56
Autores: Calado,R.T.; Franco,R.F.; Pazin-Filho,A.; Simões,M.V.; Marin-Neto,J.A.; Zago,M.A.
Although iron can catalyze the production of free radicals involved in LDL lipid peroxidation, the contribution of iron overload to atherosclerosis remains controversial. The description of two mutations in the HFE gene (Cys282Tyr and His63Asp) related to hereditary hemochromatosis provides an opportunity to address the question of the association between iron overload and atherosclerosis. We investigated the prevalence of HFE mutations in 160 survivors of myocardial infarction with angiographically demonstrated severe coronary atherosclerotic disease, and in 160 age-, gender- and race-matched healthy control subjects. PCR amplification of genomic DNA followed by RsaI and BclI restriction enzyme digestion was used to determine the genotypes. The frequency...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemochromatosis; HFE gene; Atherosclerosis; Myocardial infarction; Risk factor.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300007

Lipid core nanoparticles resembling low-density lipoprotein and regression of atherosclerotic lesions: effects of particle size
Provedor de dados: 56
Autores: Freitas,S.C.M.P.; Tavares,E.R.; Silva,B.M.O.; Meneghini,B.C.; Kalil-Filho,R.; Maranhão,R.C.
Particles are usually polydispersed and size is an important feature for lipid-based drug delivery systems in order to optimize cell-particle interactions as to pharmacologic action and toxicity. Lipid nanoparticles (LDE) with composition similar to that of low-density lipoprotein carrying paclitaxel were shown to markedly reduce atherosclerosis lesions induced in rabbits by cholesterol feeding. The aim of this study was to test whether two LDE fractions, one with small (20–60 nm) and the other with large (60–100 nm) particles, had different actions on the atherosclerotic lesions. The two LDE-paclitaxel fractions, prepared by microfluidization, were separated by density gradient ultracentrifugation and injected (4 mg/body weight, intravenously once a week)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solid lipid nanoparticles; Drug targeting; Paclitaxel; Atherosclerosis; Particle size.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300612

Effect of sexual steroids on the calcium content of aortic atherosclerotic plaque of oophorectomized rabbits
Provedor de dados: 56
Autores: Aldrighi,J.M.; Lanz,J.R.; Mansur,A.P.; Martinez,T.L.R.; Ramires,J.A.F.
We determined the effect of conjugated equine estrogen plus medroxyprogesterone acetate on calcium content of aortic atherosclerotic lesions in oophorectomized adult New Zealand rabbits submitted to a cholesterol rich diet. Five groups of 10 animals each were studied: G1 = control, G2 = cholesterol diet only, G3 = diet plus conjugated equine estrogen (0.625 mg/day); G4 and G5 = diet, conjugated equine estrogen (0.625 mg/day) plus medroxyprogesterone acetate (5 and 10 mg/day, respectively). Mean weight varied from 2.7 ± 0.27 to 3.1 ± 0.20 kg (P = 0.38) between groups at the beginning and 3.1 ± 0.27 to 3.5 ± 0.20 kg (P = 0.35) at the end of the experiment. Cholesterol and triglyceride levels were determined at the time of oophorectomy, 21 days after surgery...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estrogens; Progestagens; Calcium; Atherosclerosis; Oophorectomy; Aortic plaque area.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500007

Ox-LDL increases OX40L in endothelial cells through a LOX-1-dependent mechanism
Provedor de dados: 56
Autores: Dong,Q.; Xiang,R.; Zhang,D.Y.; Qin,S.
Oxidative low-density lipoprotein (Ox-LDL) is a key risk factor for the development of atherosclerosis, and it can stimulate the expression of a variety of inflammatory signals. As a new and highly sensitive inflammation index, OX40L may be a key to understanding the mechanisms that regulate interactions between cells within the vessel wall and inflammatory mediators during the development of atherosclerosis. To investigate whether Ox-LDL regulates OX40L expression through an oxidized LDL-1 receptor (LOX-1)-mediated mechanism, we investigated the effect of different concentrations of Ox-LDL (50, 100, 150 µg/mL) on endothelial cell proliferation and apoptosis. Stimulation with Ox-LDL increased OX40L protein 1.44-fold and mRNA 4.0-fold in endothelial cells,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Ox-LDL; LOX-1; OX40/OX40L; Polyinosinic acid.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900765

Six years of treatment with the HELP system of a patient with familial hypercholesterolemia
Provedor de dados: 56
Autores: Nascimento,M.M.; Pasqual,D.D.; Santos,J.E. dos; Riella,M.C.
The purpose of the present report is to demonstrate the long-term efficacy and safety of heparin-induced extracorporeal lipoprotein precipitation (HELP) of LDL-c and fibrinogen in the management of familial hypercholesterolemia. From June 1992 to June 1998 a 22-year-old young male patient with familial hypercholesterolemia (double heterozygote for C660X and S305C) resistant to medication and diet and with symptomatic coronary artery disease (angina) was treated weekly with 90-min sessions of the HELP system. The patient had also been previously submitted to right coronary artery angioplasty. The efficacy of the method was evaluated by comparing the reduction of total cholesterol, LDL-c and fibrinogen before and after the sessions and before and after...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Atherosclerosis; Apheresis; Coronary artery disease.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700003

Detection of Mycoplasma pneumoniae and Chlamydia pneumoniae in ruptured atherosclerotic plaques
Provedor de dados: 56
Autores: Higuchi,M.L.; Sambiase,N.; Palomino,S.; Gutierrez,P.; Demarchi,L.M.; Aiello,V.D.; Ramires,J.A.F.
This paper reports what is apparently the first observation of Mycoplasma pneumoniae in association with Chlamydia pneumoniae in thrombosed ruptured atheromas. We performed electron microscopy and in situ hybridization in specimens from three patients who died of acute myocardial infarction. These patients had typical symptoms of acute ischemic syndrome. Mycoplasmas were present mainly in the lipid core of the ruptured thrombosed plaque. Vulnerable atheromas are rich in cholesterol and may favor the growth of mycoplasmas, the only microorganisms that require cholesterol for survival. We suggest that the association of Mycoplasma pneumoniae and Chlamydia pneumoniae may increase the virulence of these microorganisms, favoring proliferation, plaque...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mycoplasma pneumoniae; Chlamydia pneumoniae; Atherosclerosis; Vulnerable plaque; Inflammation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900005

The action of red wine and purple grape juice on vascular reactivity is independent of plasma lipids in hypercholesterolemic patients
Provedor de dados: 56
Autores: Coimbra,S.R.; Lage,S.H.; Brandizzi,L.; Yoshida,V.; da Luz,P.L.
Although red wine (RW) reduces cardiovascular risk, the mechanisms underlying the effect have not been identified. Correction of endothelial dysfunction by RW flavonoids could be one mechanism. We measured brachial artery reactivity by high-resolution ultrasonography, plasma lipids, glucose, adhesion molecules (ICAM-1 and VCAM), and platelet function in 16 hypercholesterolemic individuals (8 men and 8 women; mean age 51.6 ± 8.1 years) without other risk factors. Twenty-four normal subjects were used as controls for vascular reactivity. Subjects randomly received RW, 250 ml/day, or purple grape juice (GJ), 500 ml/day, for 14 days with an equal wash-out period. At baseline, all 16 subjects were hypercholesterolemic (mean LDL = 181.0 ± 28.7 mg/dl) but HDL,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Endothelial function; Flavonoids; Alcohol; Red wine and grape juice; Brachial artery dilation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900008

Pitfalls in the assessment of murine atherosclerosis
Provedor de dados: 56
Autores: Catanozi,S.; Rocha,J.C.; Passarelli,M.; Chiquito,F.C.; Quintão,E.C.R.; Nakandakare,E.R.
This review provides examples of the fact that different procedures for the measurement of atherosclerosis in mice may lead to interpretation of the extent of atherosclerosis having markedly different biological and clinical significance for humans: 1) aortic cholesterol measurement is highly sensitive for the detection of early and advanced atherosclerosis lesions, but misses the identification of the location and complexity of these lesions that are so critical for humans; 2) the histological analysis of the aortic root lesions in simvastatin-treated and control mice reveals similar lesion morphology in spite of the remarkable simvastatin-induced reduction of the aortic cholesteryl ester content; 3) in histological analyses, chemical fixation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Animal models; Disease models; Aorta; Pathology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600001

Paradoxical effect of a pequi oil-rich diet on the development of atherosclerosis: balance between antioxidant and hyperlipidemic properties
Provedor de dados: 56
Autores: Aguilar,E.C.; Jascolka,T.L.; Teixeira,L.G.; Lages,P.C.; Ribeiro,A.C.C.; Vieira,E.L.M.; Peluzio,M.C.G.; Alvarez-Leite,J.I.
Pequi is the fruit of Caryocar brasiliense and its oil has a high concentration of monounsaturated and saturated fatty acids, which are anti- and pro-atherogenic agents, respectively, and of carotenoids, which give it antioxidant properties. Our objective was to study the effect of the intake of a cholesterol-rich diet supplemented with pequi oil, compared to the same diet containing soybean oil, on atherosclerosis development, and oxidative stress in atherosclerosis-susceptible LDL receptor-deficient mice (LDLr-/-, C57BL/6-background). Female mice were fed a cholesterol-rich diet containing 7% soybean oil (Soybean group, N = 12) or 7% pequi oil (Pequi group, N = 12) for 6 weeks. The Pequi group presented a more atherogenic lipid profile and more advanced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pequi oil; Fatty acid; Lipids; Atherosclerosis; Oxidative stress; Cholesterol.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700006

Allantoin as an independent marker associated with carotid intima-media thickness in subclinical atherosclerosis
Provedor de dados: 56
Autores: Santana,M.S.; Nascimento,K.P.; Lotufo,P.A.; Benseñor,I.M.; Meotti,F.C.
Allantoin is the main product of uric acid oxidation and was found to be augmented in atherosclerotic plaque in human autopsy and in animal models of atherosclerosis. Uric acid is abundant in human plasma and is prone to oxidation in inflammatory conditions such as atherosclerosis. In this study, we found a significant increase in plasma uric acid (P=0.002) and allantoin (P=0.025) in participants of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) that presented common carotid intima-media thickness (c-IMT) within the 75th percentile (c-IMT≥P75). Multiple linear regression showed an association of c-IMT with uric acid (β=0.0004, P=0.014) and allantoin (β=0.018, P=0.008). This association was independent of age, the traditional risk factor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allantoin; Uric acid; Common carotid intima-media thickness; Atherosclerosis; Oxidation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800613

Detection of human cytomegalovirus and Epstein-Barr virus in coronary atherosclerotic tissue
Provedor de dados: 58
Autores: Imbronito,Ana Vitória; Marcelino,Silvia Linardi; Grande,Sabrina Rosa; Nunes,Fabio Daumas; Romito,Giuseppe Alexandre.
Previous studies indicated that patients with atherosclerosis are predominantly infected by human cytomegalovirus (HCMV), but rarely infected by type 1 Epstein-Barr virus (EBV-1). In this study, atheromas of 30 patients who underwent aortocoronary bypass surgery with coronary endartherectomy were tested for the presence of these two viruses. HCMV occurred in 93.3% of the samples and EBV-1 was present in 50% of them. Concurrent presence of both pathogens was detected in 43.3% of the samples.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Cytomegalovirus; Epstein-Barr virus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300004