Consulta: subjectFacets:"Bacteroidales"
Registros recuperados: 6
Data/hora: 05/06/2024 02:29:23

Development of microbial and chemical MST tools to identify the origin of the faecal pollution in bathing and shellfish harvesting waters in France
Provedor de dados: 5
Autores: Gourmelon, Michele; Caprais, Marie-paule; Mieszkin, Sophie; Marti, Romain; Wery, Nathalie; Jarde, E.; Derrien, Marc; Jadas-hecart, A.; Communal, P. Y.; Jaffrezic, A.; Pourcher, A. M.
The microbiological quality of coastal or river waters can be affected by faecal pollution from human or animal sources. An efficient MST (Microbial Source Tracking) toolbox consisting of several host-specific markers would therefore be valuable for identifying the origin of the faecal pollution in the environment and thus for effective resource management and remediation. In this multidisciplinary study, after having tested some MST markers on faecal samples, we compared a selection of 17 parameters corresponding to chemical (steroid ratios, caffeine, and synthetic compounds), bacterial (host-specific Bacteroidales, Lactobacillus amylovorus and Bifidobacterium adolescentis) and viral (genotypes I-IV of F-specific bacteriophages, FRNAPH) markers on...
Tipo: Text
Palavras-chave: Microbial source tracking; Faecal pollution; Bacteroidales; Bifidobacterium adolescentis; Lactobacillus; FRNA bacteriophages; Steroids; Caffeine.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00016/12710/9690.pdf

Identification de l'origine de la contamination observée en baie de La Baule : Première partie de l'étude
Provedor de dados: 5
Autores: Caprais, Marie-paule; Gourmelon, Michele; Le Mennec, Cecile; Joubrel, Rozenn.
Afin d'améliorer la qualité sanitaire des coquillages du secteur La Baule - Le Pouliguen, la Communauté d'Agglomération CAP Atlantique a mis en place un programme d'étude pour identifier les sources de pollution sur ce secteur et initier des actions permettant de limiter les apports d'origine fécale aux coquillages. Dans le cadre de cette présente étude, de juin 2006 à décembre 2007, deux méthodes d'identification de l'origine de la contamination fécale en cours de développement au laboratoire de microbiologie d'Ifremer de Brest, ont été appliquées sur des lots de coquillages (n=87 ; 6 sites) et des échantillons d'eaux (n=40 ; 7 localisations). La première méthode consistait à rechercher par PCR conventionnelle les gènes codant les ARN ribosomiques (ARNr)...
Tipo: Text
Palavras-chave: Bacteroidales; Bactériophages F +ARN spécifiques; Marqueurs de contamination fécale; E. coli; Génotypage; PCR conventionnelle; Coquillage; Littoral.
Ano: 2008
URL: https://archimer.ifremer.fr/doc/00622/73452/72691.pdf

Recent innovation in microbial source tracking using bacterial real-time PCR markers in shellfish
Provedor de dados: 5
Autores: Mauffret, Aourell; Mieszkin, Sophie; Morizur, Mael; Alfiansah, Yustian Rovi; Lozach, Solen; Gourmelon, Michele.
We assessed the capacity of real-time PCR markers to identify the origin of contamination in shellfish. Oyster, cockles or clams were either contaminated with fecal materials and host-associated markers designed from Bacteroidales or Catellicoccus marimammalium 16S RNA genes were extracted from their intravalvular liquid, digestive tissues or shellfish flesh. Extraction of bacterial DNA from the oyster intravalvular liquid with FastDNA spin kit for soil enabled the selected markers to be quantified in 100% of artificially contaminated samples, and the source of contamination to be identified in 13 out of 38 naturally contaminated batches from European Class B and Class C areas. However, this protocol did not enable the origin of the contamination to be...
Tipo: Text
Palavras-chave: Microbial source tracking; Shellfish; Bacteroidales; Real-time PCR; Intravalvular liquid; Digestive tissues.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24776/25188.pdf

Phylogenetic analysis of Bacteroidales 16S rRNA gene sequences from human and animal effluents and assessment of ruminant faecal pollution by real-time PCR
Provedor de dados: 5
Autores: Mieszkin, Sophie; Yala, Jean Fabrice; Joubrel, Rozenn; Gourmelon, Michele.
Aims: The aims of this study were to evaluate the host-specific distribution of Bacteroidales 16S rRNA gene sequences from human- and animal-related effluents and faeces, and to define a ruminant-specific marker. Methods and Results: Bacteroidales 16S rRNA gene clone libraries were constructed from samples of effluent (sewage, bovine manure and pig slurry) and faeces (human, bovine, pig and wild bird), using PCR primers targeting order Bacteroidales. The phylogenetic analysis revealed six main distinct human-, bovine-, pig- and wild bird-specific clusters. From the bovine-specific cluster II, we designed a ruminant-specific marker, Rum-2-Bac, and this showed 97% sensitivity (n = 30) and 100% specificity (n = 40) when tested by TaqMan (R) real-time PCR....
Tipo: Text
Palavras-chave: 16S rRNA gene; Bacteroidales; Faecal contamination; Microbial Source Tracking; Sewage and manure.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00000/11156/8235.pdf

Diagnostic moléculaire de l'origine des contaminations fécales dans l'environnement littoral - Développement de marqueurs Bacteroidales spécifiques de l'hôte
Provedor de dados: 5
Autores: Mieszkin, Sophie.
Human and animal faecal pollution affects environmental water in inland and coastal areas, with negative implications for recreational uses, public safety and shellfish sanitary status due to the presence of enteric pathogens. Starting from 2011, the revised Bathing Water European Directive (2006/7/CE) requires the establishment of bathing water profiles with an inventory and study of the pollution sources likely to affect water quality. The faecal microbiological indicators used in these regulations, Escherichia coli and enteroccoci, cannot distinguish between human and animal faecal contamination. Thus, alternative methods, that focus on target microorganisms such as bacteria belonging to the Bacteroidales order were developed or are being developed to...
Tipo: Text
Palavras-chave: Contaminations fécales humaines; Porcines et ruminants; Bacteroidales; Gènes codant les ARNr 16S; PCR quantitative en temps réel; Eaux; Persistance; Huîtres; Human; Porcine and ruminant faecal contaminations; Bacteroidales; 16S rRNA genes; Quantitative real-time PCR; Water; Persistence; Oyster.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00021/13206/10258.pdf

Application of library-independent microbial source tracking methods for identifying the sources of faecal contamination in coastal areas
Provedor de dados: 5
Autores: Gourmelon, Michele; Caprais, Marie-paule; Le Mennec, Cecile; Mieszkin, Sophie; Ponthoreau, C.; Gendronneau, M.
Faecal contamination sources were identified in coastal areas around the Guerande-Atlantique peninsula using two microbial source tracking (MST) methods: (i) Bacteroidales host-specific 16S rRNA gene markers measured by real-time PCR and (ii) F-specific bacteriophage (FRNAPH) genotyping. Both methods were used on 63 water samples from 7 water courses. HF183 marker and bacteriophage genogroup II (FRNAPH II) were detected in all water samples and in the majority of water samples, respectively, from La Torre stream (W5), Piriac (W2), R2000 (W3) and Mazy (W7) rain water drains, and also detected, less frequently, in Le Nau drain (W4), suggesting contamination by human faecal sources at these sites. These human markers were weakly detected in Pouliguen channel...
Tipo: Text
Palavras-chave: Bacteroidales; F-specific RNA bacteriophages; Faecal bacterial indicators; Microbial source tracking; Water.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11384/10304.pdf