Consulta: subjectFacets:"Biofilms"
Registros recuperados: 27
Data/hora: 01/06/2024 08:19:22

Formación de biopelículas y resistencia a desinfectantes en cepas de salmonella aisladas de nopal verdura y muestras de agua y suelo de uso agrícola.
Provedor de dados: 32
Autores: Santos Villamil, Alfricia Adriana de los.
La presencia de Salmonella en nopal verdura fresco (Opuntia ficus-indica L.) MILL se ha documentado recientemente (Hernández et al., 2009). La contaminación de alimentos es un problema de salud pública que afecta a las personas en todo el mundo. Una característica importante de Salmonella es su capacidad para formar biopelículas, las cuales son comunidades de células microbianas embebidas en una matriz de exopolímeros que ellas mismas producen en respuesta a factores como la temperatura, la humedad relativa y la composición del medio en que se encuentran (Annous et al., 2005; Stepanovic et al., 2000). Esta capacidad le permite a Salmonella adherirse de manera irreversible y de colonizar superficies bióticas y abióticas (Annous et al., 2005, Stepanovic et...
Palavras-chave: Biopelículas; Desinfectantes; Nopal; Salmonella; Biofilms; Desinfectants; Maestría; Fitopatología.
Ano: 2010
URL: http://hdl.handle.net/10521/220

Formación de biopelículas y resistencia a desinfectantes en cepas de salmonella aisladas de nopal verdura y muestras de agua y suelo de uso agrícola.
Provedor de dados: 32
Autores: Santos Villamil, Alfricia Adriana de los.
La presencia de Salmonella en nopal verdura fresco (Opuntia ficus-indica L.) MILL se ha documentado recientemente (Hernández et al., 2009). La contaminación de alimentos es un problema de salud pública que afecta a las personas en todo el mundo. Una característica importante de Salmonella es su capacidad para formar biopelículas, las cuales son comunidades de células microbianas embebidas en una matriz de exopolímeros que ellas mismas producen en respuesta a factores como la temperatura, la humedad relativa y la composición del medio en que se encuentran (Annous et al., 2005; Stepanovic et al., 2000). Esta capacidad le permite a Salmonella adherirse de manera irreversible y de colonizar superficies bióticas y abióticas (Annous et al., 2005, Stepanovic et...
Palavras-chave: Biopelículas; Desinfectantes; Nopal; Salmonella; Biofilms; Desinfectants; Maestría; Fitopatología.
Ano: 2010
URL: http://hdl.handle.net/10521/220

Computational analysis suggests that virulence of Chromobacterium violaceum might be linked to biofilm formation and poly-NAG biosynthesis
Provedor de dados: 74
Autores: Becker,Sidnei; Soares,Cíntia; Porto,Luismar Marques.
Groups of genes that produce exopolysaccharide with a N-acetyl-D-glucosamine monomer are in the genome of several pathogenic bacteria. Chromobacterium violaceum, an opportunistic pathogen, has the operon hmsHFR-CV2940, whose proteins can synthesize such polysaccharide. In this work, multiple alignments among proteins from bacteria that synthesize such polysaccharide were used to verify the existence of amino acids that might be critical for pathogen activity. Three-dimensional models were generated for spatial visualization of these amino acid residues. The analysis carried out showed that the protein HmsR preserves the amino acids D135, D228, Q264 and R267, considered critical for the formation of biofilms and, furthermore, that these amino acids are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Exopolysaccharide; Chromobacterium violaceum pathogenicity; Comparative genomics.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300031

Prevalence and Characterization of Shiga Toxin-Producing and Enteropathogenic Escherichia coli in Shellfish-Harvesting Areas and Their Watersheds
Provedor de dados: 5
Autores: Baliere, Charlotte; Rince, Alain; Blanco, Jorge; Dahbi, Ghizlane; Harei, Josee; Vogeleer, Philippe; Giard, Jean-christophe; Mariani-kurkdfian-, Patricia; Gourmelon, Michele.
Tipo: Text
Palavras-chave: STEC; EPEC; Shellfish; Water; MLST; PFGE; Biofilms.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00298/40904/39949.pdf

Low exposure levels of urban metals induce heterotrophic community tolerance: a microcosm validation
Provedor de dados: 5
Autores: Fechner, Lise C.; Gourlay-france, Catherine; Tusseau-vuillemin, Marie-helene.
The biological response of periphyton chronically exposed to metals of urban origin (Cd, Ni and Zn) was investigated with a Pollution-Induced Community Tolerance (PICT) approach using a previously developed short-term toxicity test based on beta-glucosidase (heterotrophic) activity. Periphyton was grown on plastic membranes immersed in indoor aquaria contaminated with metals at realistic contamination levels (0.3, 3 mu g/l for Cd, 5, 50 mu g/l for Ni, 20, 200 mu g/l for Zn). After 3 weeks of exposure, biofilms' parameters (dry-weight, chlorophyll a concentration, heterotrophic activity) were analyzed and tolerance acquisition of the heterotrophic communities was assessed using the toxicity test. Modifications of bacterial and eukaryotic community structure...
Tipo: Text
Palavras-chave: Biofilms; Urban metal; Community tolerance; ARISA.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00036/14682/12028.pdf

Factors Involved in the Persistence of a Shiga Toxin-Producing Escherichia coil O157:H7 Strain in Bovine Feces and Gastro-Intestinal Content
Provedor de dados: 5
Autores: Segura, Audrey; Auffret, Pauline; Bibbal, Delphine; Bertoni, Marine; Durand, Alexandra; Jubelin, Gregory; Kerouredan, Monique; Brugere, Hubert; Bertin, Yolande; Forano, Evelyne.
Healthy cattle are the primary reservoir for O157:H7 Shiga toxin-producing E coil responsible for human food-borne infections. Because farm environment acts as a source of cattle contamination, it is important to better understand the factors controlling the persistence of E. coil O157:H7 outside the bovine gut. The E. coil O157:H7 strain MC2, identified as a persistent strain in French farms, possessed the characteristics required to cause human infections and genetic markers associated with clinical O157:H7 isolates. Therefore, the capacity of E. coil MC2 to survive during its transit through the bovine gastro-intestinal tract (GIT) and to respond to stresses potentially encountered in extra intestinal environments was analyzed. E. coil MC2 survived in...
Tipo: Text
Palavras-chave: Escherichia coli; STEC; EHEC; O157:H7; Biofilms; Stress response; Persistence.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00434/54588/55972.pdf

Extended Spectrum Beta-Lactamase Production and Biofilm Formation in Salmonella Serovars Resistant to Antimicrobial Agents
Provedor de dados: 89
Autores: Mandelli,JZA; Ehrhardt,A; Manto,L; Borges,KA; Furian,TQ; Weber,B; Rodrigues,LB; Santos,LR.
ABSTRACT Antimicrobial resistance is a serious public health problem and Salmonella spp. is highly resistant to antimicrobial agents. Biofilms are important in the food industry due to their formation on products, utensils, and surfaces and the difficulty in their removal. The objective of this study was to assess extended spectrum beta-lactamase (ESBL) production, antimicrobial resistance, and biofilm production of Salmonella isolated from poultry slaughterhouses. Antimicrobial susceptibility was assessed by the disk diffusion assay and ESBL by double diffusion disk assay using the beta-lactamase inhibitor (amoxicillin+clavulanate). The antimicrobials tested were: ampicillin, amoxicillin+clavulanate, aztreonam, ceftazidime, cefotaxime, chloramphenicol,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Drug resistance; ESBL; Salmonella.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200348

Microbial contamination of stored hydrocarbon fuels and its control
Provedor de dados: 59
Autores: Gaylarde,Christine C.; Bento,Fátima M.; Kelley,Joan.
The major microbial problem in the petroleum refining industry is contamination of stored products, which can lead to loss of product quality, formation of sludge and deterioration of pipework and storage tanks, both in the refinery and at the end-user. Three major classes of fuel are discussed in this article - gasoline, aviation kerosene and diesel, corresponding to increasingly heavy petroleum fractions. The fuel that presents the most serious microbiological problems is diesel. The many microorganisms that have been isolated from hydrocarbon fuel systems are listed. The conditions required for microbial growth and the methods used to monitor and to control this activity are discussed. The effects of various fuel additives, including biocides, are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biocides; Biofilms; Hydrocarbon fuels; Microbial corrosion; Storage tanks.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100001

Detección de microorganismos potencialmente patógenos en hogares de Mar del Plata
Provedor de dados: 38
Autores: Gómez,Diana; Lavayén,Silvina; Nario,Flavia; Piquin,Andrea; Zotta,Claudio Marcelo.
La cocina y el baño son los lugares del hogar más susceptibles de contaminación microbiana. Estos microbios se depositan sobre las superficies formando biofilms y pueden constituirse en potenciales fuentes causales de enfermedades. El objetivo del trabajo fue detectar la presencia y la concentración de carga microbiana en los elementos y utensilios de los sitios más críticos de viviendas de la ciudad de Mar del Plata. Se recolectaron 478 muestras de elementos y utensilios de cocinas y baños provenientes de 36 hogares de familias de características socioeconómicas similares a los casos de Síndrome Urémico Hemolítico ocurridos en la misma ciudad. En cocinas el 52% superó los parámetros establecidos para el estudio detectándose &gt;100 UFC/muestra de aerobios...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carga microbiana; Hogar; Biofilms; Síndrome urémico hemolítico.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572011000300005

Bactericidia de hipoclorito de sodio sobre Staphylococcus cohnii productor de biofilm en una fabrica
Provedor de dados: 38
Autores: Porcel de Fernandez,Norma Dolores; Urueña,Raquel; Gaudioso de Allori,Maria Cristina; de Castillo,Marta Elena Cecilia.
En equipamientos industriales la persistencia de las bacterias se debe al desarrollo de biofilms. El hipoclorito de sodio (NaClO) es usado rutinariamente como desinfectante. El objetivo del trabajo fue determinar el efecto inhibitorio del NaClO sobre S. cohnii productoras y no productoras de biofilms aisladas de una fabrica de pastas a partir de maquinarias de un establecimiento de Tucuman, Argentina. Se estudio produccion de biofilm, concentracion inhibitoria minima (CIM), concentracion bactericida minima (CBM) y curva de tiempo de muerte con NaClO frente a S. cohnii productora y no productora de biofilm. Los resultados obtenidos demostraron que de los ocho cocos grampositivos aislados, cuatro correspondieron a S. cohnii. Los valores CIM y CBM para S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus cohnii; Biofilms; Hipoclorito de sodio; Actividad antimicrobiana.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572013000400007

Pseudomonas Aeruginosa: Virulence Factors and Antibiotic Resistance Genes
Provedor de dados: 52
Autores: Rocha,Antônio José; Barsottini,Mario Ramos de Oliveira; Rocha,Renan Rhonalty; Laurindo,Maria Vitória; Moraes,Francisco Leandro Laurindo de; Rocha,Soraya Lília da.
Abstract In this review, we explore some aspects of Pseudomonas aeruginosa virulence factors that are related to disease development in healthy organisms and resistance to antibiotics. This pathogen is one of the most clinically and epidemiologically important bacteria in Brazil, being the major cause of opportunistic infections. Among the virulence factors, biofilm formation acting of manner different in the organism. Furthermore, we review several P. aeruginosa genes that act in antimicrobial resistance, such as β-lactamases against β-lactamers. The resistance to pied-lactamases in P. aeruginosa is associated to resistance to the broad-spectrum cephalosporin. On the other hand, there is a group of synthetic broad-spectrum antibiotics acting on DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Virulence factors; Antimicrobial activities; Biofilms; Β-lactamase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100902

Influence of growth media and temperature on bacterial adhesion to polystyrene surfaces
Provedor de dados: 52
Autores: Zeraik,Ana Eliza; Nitschke,Marcia.
Bacterial adhesion to inert surfaces is a complex process influenced by environmental conditions. In this work, the influence of growth medium and temperature on the adhesion of Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Micrococcus luteus and Listeria monocytogenes to polystyrene surfaces was studied. Most bacteria demonstrated the highest adhesion when cultured in TSYEA, except S. marcescens, which showed to be positively influenced by the pigment production, favored in poor nutrient media (lactose and peptone agar). P. aeruginosa adhesion to polystyrene increased at low temperatures whatever the medium used. The culture medium influenced the surface properties of the bacteria as assessed by the MATS test.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial adhesion; Biofilms; Hydrophobicity; Polystyrene.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400012

Biofilms: microbes and disease
Provedor de dados: 55
Autores: Aparna,Madhu Sharma; Yadav,Sarita.
Bacteria that attach to surface aggregate in a hydrated polymeric matrix of their own synthesis to form biofilms. These represent microbial societies with their own defense and communication system. Transitioning from acute to chronic infection is frequently associated with biofilm formation.Bacteria in biofilms are innately more resistant to antimicrobial agents. The presence of indwelling medical devices increases the risk for biofilm formation and subsequent infection. The current antibiotic therapies are of limited effectiveness in resolving biofilms infection.This review attempts to discuss the stages in biofilm formation, their pathogenic mechanisms, effect of antimicrobial agents, detection and eradication of the biofilms.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Staphylococci; Pseudomonas; Candida.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600016

Evaluation of different detection methods of biofilm formation in the clinical isolates
Provedor de dados: 55
Autores: Hassan,Afreenish; Usman,Javaid; Kaleem,Fatima; Omair,Maria; Khalid,Ali; Iqbal,Muhammad.
BACKGROUND: Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. There are various methods to detect biofilm production like Tissue Culture Plate (TCP), Tube method (TM), Congo Red Agar method (CRA), bioluminescent assay, piezoelectric sensors, and fluorescent microscopic examination. OBJECTIVE: This study was conducted to compare three methods for the detection of biofilms. METHOD: The study was carried out at the Department of Microbiology, Army Medical College, National University of Sciences and Technology, Pakistan, from January 2010 to June 2010. A total of 110 clinical isolates were subjected to biofilm detection methods. Isolates were identified by standard...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Bacteria; Anti-bacterial agents.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400002

Biofilm formation by Staphylococcus aureus and Salmonella spp. under mono and dual-species conditions and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite
Provedor de dados: 58
Autores: Iñiguez-Moreno,Maricarmen; Gutiérrez-Lomelí,Melesio; Guerrero-Medina,Pedro Javier; Avila-Novoa,María Guadalupe.
Abstract The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37 °C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p &gt; 0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp. was favored, 7.61 ± 0.13 Log10 CFU/cm2, compared with monospecies biofilms onto the same surface, 5.91 ± 0.44 Log10 CFU/cm2 (p &lt; 0.05). The mono and dual-species biofilms were subjected to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Salmonella spp.; Staphylococcus aureus; Disinfectants; Sensitivity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200310

Long-term evaluation of the antimicrobial susceptibility and microbial profile of subgingival biofilms in individuals with aggressive periodontitis
Provedor de dados: 58
Autores: Lourenço,Talita Gomes Baêta; Heller,Débora; Souto,Renata Martins do; Silva-Senem,Mayra Xavier e; Varela,Victor Macedo; Torres,Maria Cynesia Barros; Feres-Filho,Eduardo Jorge; Colombo,Ana Paula Vieira.
This study evaluates the antimicrobial susceptibility and composition of subgingival biofilms in generalized aggressive periodontitis (GAP) patients treated using mechanical/antimicrobial therapies, including chlorhexidine (CHX), amoxicillin (AMX) and metronidazole (MET). GAP patients allocated to the placebo (C, n = 15) or test group (T, n = 16) received full-mouth disinfection with CHX, scaling and root planning, and systemic AMX (500 mg)/MET (250 mg) or placebos. Subgingival plaque samples were obtained at baseline, 3, 6, 9 and 12 months post-therapy from 3–4 periodontal pockets, and the samples were pooled and cultivated under anaerobic conditions. The minimum inhibitory concentrations (MICs) of AMX, MET and CHX were assessed using the microdilution...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aggressive periodontitis; Biofilms; Microbial sensitivity tests; DNA probes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200493

Effect of alcohols on filamentation, growth, viability and biofilm development in Candida albicans
Provedor de dados: 58
Autores: Chauhan,Nitin M; Shinde,Ravikumar B; Karuppayil,S. Mohan.
In this study we report the potential of alcohols as morphogenetic regulators in Candida albicans. All the alcohols tested influenced various modes of growth like planktonic as well as biofilm forms. Viability was affected at high concentrations. Among the alcohols, the response of C. albicans to amyl alcohol (pentanol) was noteworthy. Amyl alcohol at a concentration 0.5% which was not inhibitory to growth and viability specifically inhibited morphogenetic switching from yeast to hyphal forms. It also inhibited normal biofilm development favoring yeast dominated biofilms. Based on this study we hypothesize that alcohols produced under anaerobic conditions may not favor biofilm development and support dissemination of yeast cells. Since anaerobic conditions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcohols; Signalling; Morphogenesis; Biofilms; Yeast metabolism; Candida albicans.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400041

Isolation of Pseudomonas aeruginosa strains from dental office environments and units in Barretos, state of São Paulo, Brazil, and analysis of their susceptibility to antimicrobial drugs
Provedor de dados: 58
Autores: Oliveira,Ana Claudia de; Maluta,Renato Pariz; Stella,Ariel Eurides; Rigobelo,Everlon Cid; Marin,José Moacir; Ávila,Fernando Antonio de.
A wide variety of opportunistic pathogens has been detected in the tubing supplying water to odontological equipment, in special in the biofilm lining of these tubes. Among these pathogens, Pseudomonas aeruginosa, one of the leading causes of nosocomial infections, is frequently found in water lines supplying dental units. In the present work, 160 samples of water, and 200 fomite samples from forty dental units were collected in the city of Barretos, State of São Paulo, Brazil and evaluated between January and July, 2005. Seventy-six P. aeruginosa strains, isolated from the dental environment (5 strains) and water system (71 strains), were tested for susceptibility to six antimicrobial drugs most frequently used against P. aeruginosa infections....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Dental units; Water lines; Opportunistic pathogens; Antimicrobial resistance; Biofilms.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300032

Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces
Provedor de dados: 58
Autores: Reis-Teixeira,Fernanda Barbosa dos; Alves,Virgínia Farias; Martinis,Elaine Cristina Pereira de.
Abstract The pathogenic bacterium Listeria monocytogenes can persist in food processing plants for many years, even when appropriate hygienic measures are in place, with potential for contaminating ready-to-eat products and, its ability to form biofilms on abiotic surfaces certainly contributes for the environmental persistence. In this research, L. monocytogenes was grown in biofilms up 8 days attached to stainless steel and glass surfaces, contributing for advancing the knowledge on architecture of mature biofilms, since many literature studies carried out on this topic considered only early stages of cell adhesion. In this study, biofilm populations of two strains of L. monocytogenes (serotypes 1/2a and 4b) on stainless steel coupons and glass were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Abiotic surfaces; Persistence; Biofilms.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300587

Different methods to quantify Listeria monocytogenesbiofilms cells showed different profile in their viability
Provedor de dados: 58
Autores: Winkelströter,Lizziane Kretli; Martinis,Elaine C.P. De.
Listeria monocytogenes is a foodborne pathogen able to adhere and to form biofilms in several materials commonly present in food processing plants. The aim of this study was to evaluate the resistance of Listeria monocytogenes attached to abiotic surface, after treatment with sanitizers, by culture method, microscopy and Quantitative Real Time Polymerase Chain Reaction (qPCR). Biofilms of L. monocytogenes were obtained in stainless steel coupons immersed in Brain Heart Infusion Broth, under agitation at 37 °C for 24 h. The methods selected for this study were based on plate count, microscopic count with the aid of viability dyes (CTC-DAPI), and qPCR. Results of culture method showed that peroxyacetic acid was efficient to kill sessile L. monocytogenes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; L. monocytogenes; QPCR; Peroxyacetic acid; Sodium hypochlorite.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100231

Newly-synthesized chalcones-inhibition of adherence and biofilm formation of methicillin-resistant Staphylococcus aureus
Provedor de dados: 58
Autores: Bozic,Dragana D.; Milenkovic,Marina; Ivkovic,Branka; Cirkovic,Ivana.
Biofilm formation and adherence of bacteria to host tissue are one of the most important virulence factors of methicillin-resistant strains of Staphylococcus aureus (MRSA). The number of resistant strains is seriously increasing during the past years and bacteria have become resistant, not only to methicillin, but also to other commonly used antistaphylococcal antibiotics. There is a great need for discovering a novel antimicrobial agent for the treatment of staphylococcal infections. One of the most promising groups of compounds appears to be chalcones. In present study we evaluated the in vitro effect of three newly synthesized chalcones: 1,3-Bis-(2-hydroxy-phenyl)-propenone, 3-(3Hydroxy-phenyl)-1-(2-hydroxy-phenyl)-propenone and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chalcones; Glycocalyx; Biofilms; MRSA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100038

Architecture and relevance of several strongly adhered biofilms over a polyester imide (PEI) surface
Provedor de dados: 58
Autores: Soncini-Júnior,Giancarlo; Franchetti,Sandra Mara Martins; Marconato,José Carlos.
A microscopical study was undertaken to analyze the eventual fungi adhesion over a polyester imide surface in enameled copper wires. Scanning electron microscopy allowed to observe in these adhered biofilms, a high amount of pigments, hyphae and its enzymatic arsenal probably acting in the surface of the polymer. Due to the highly aromatic nature of this polyester and the residual phenol derivates still present in the reticulated polymer, an antifungal activity could be expected, but no significant changes were noted in fungal growing and in the adhesion process. Additionally, the wires showed completely failure in all insulation properties. The studies aimed to understand and evaluate the great fungi potential that could be explored in biodeterioration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungi; Biofilms; Hyphae; Pigments; Adhesion; Scanning electron microscopy; Polymeric surface; Polyester-imide; Enameled copper wires.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500036

Effects of Myrcia ovata Cambess. essential oil on planktonic growth of gastrointestinal microorganisms and biofilm formation of Enterococcus faecalis
Provedor de dados: 58
Autores: Cândido,Cinthya S.; Portella,Cadmo Silton A.; Laranjeira,Bruno J.; Silva,Sérgio S. da; Arriaga,Angela M.C.; Santiago,Gilvandete M. P.; Gomes,Geovany A.; Almeida,Paulo César; Carvalho,Cibele B. M.
The essential oil from the leaves of Myrcia ovata Cambess., commonly used in Brazil for the treatment of gastric illnesses, was screened for antimicrobial activity and action in the formation of microbial biofilms by Enterococcus faecalis. The oil was obtained by hydrodistillation using a clevenger-type system. Its chemical composition was analyzed using GC and GC-MS. Both MIC and MBC of the essential oil were determined by broth microdilution techniques and agar dilution method. The essential oil showed antimicrobial activity against E. faecalis, Escherichia coli, Pseudomonas aeruginosa, Salmonella choleraesuis, Staphylococcus aureus, Streptococcus pneumoniae and Candida parapsilosis. The results showed that the essential oil of M. ovata Cambess. was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential oil; Myrcia ovata Cambess.; Antimicrobial activity; Biofilms.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300012

Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis
Provedor de dados: 58
Autores: Melo,Poliana de Castro; Ferreira,Luciano Menezes; Nader Filho,Antônio; Zafalon,Luiz Francisco; Vicente,Hinig Isa Godoy; Souza,Viviane de.
Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Bovine mastitis; Phenotypic and molecular analysis; Staphylococcus aureus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100017

Antibiofilm activity of Cobetia marina filtrate upon Staphylococcus epidermidis catheter-related isolates
Provedor de dados: 58
Autores: Trentin,D.S; Gorziza,D. F; Abraham,W.R; Antunes,A.L.S; Lerner,C; Mothes,B; Termignoni,C; Macedo,A.J.
We report the antibiofilm activity by the sponge-associated bacterium Cobetia marina upon Staphylococcus epidermidis clinical isolates obtained from central venous catheters. Antibiofilm activity/antimicrobial susceptibility correlation might predict the action of the metabolite(s) upon Staphylococcus epidermidis in the clinic, making it a possible adjuvant in therapies against biofilm-associated infections.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Antibiofilm activity; Staphylococcus epidermidis; Cobetia marina; Darwinella sp.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400013

Expression of virulence genes by Listeria monocytogenes J0161 in natural environment
Provedor de dados: 58
Autores: Tirumalai,Prem Saran; Prakash,Soam.
Majority of studies concerning the gene expression of Listeria monocytogenes have been done on pure culture states. Our objective was to study L.monocytogenes in a co-cultured state and to understand if microbes in their natural state of existence are different in their expression than that of the purely cultured lab grown forms. For a long period discussions have been on the expression of prfA, (which is a virulence gene regulator) in a mammalian host and its role in causing the switch from a saprophytic to pathogenic form of L.monocytogenes. We, in this paper for the first time report the expression of prfA and other virulence genes by L.monocytogenes under different extracellular conditions, and also as a pure culture biofilms, that is different from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; PrfA; Virulence; Microarray; Biofilms; Co-culture; Gene Expression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200050

Salmonella Enteritidis forms biofilm under low temperatures on different food industry surfaces
Provedor de dados: 65
Autores: Webber,Bruna; Oliveira,Amauri Picollo de; Pottker,Emanuele Serro; Daroit,Luciane; Levandowski,Rafael; Santos,Luciana Ruschel dos; Nascimento,Vladimir Pinheiro do; Rodrigues,Laura Beatriz.
ABSTRACT:  We evaluated the influence of temperature on the ability of Salmonella Enteritidis (SE) to form biofilms on stainless steel, polyethylene, and polyurethane surfaces under different hygiene procedures. These materials were placed on SE culture and incubated at 42±1 ºC, 36±1 ºC, 25±1 ºC, 9±1 ºC, and 3±1 ºC for 4, 8, 12, and 24 h. Hot water at 45 ºC and 85 ºC, 0.5% peracetic acid solution, and 1% quaternary ammonia were used for hygienization. Biofilm formation occurred at all temperatures evaluated, highlighting at 3 ºC which has not been reported as an ideal temperature for the adhesion of SE to these materials. The SE adhered more often to polyethylene surfaces than to polyurethane and stainless steel surfaces (P&lt;0.05). Peracetic acid and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella Enteritidis; Biofilms; Surfaces; Hygiene procedures; Food microbiology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700452